Acute direct adenoviral vector cytotoxicity and chronic, but not acute, inflammatory responses correlate with decreased vector-mediated transgene expression in the brain

Molecular Therapy

Volumn 3, Issue 1, 2001, Pages 36-46

  Thomas, Clare E ^a   Birkett, Darren ^a   Anozie, Ijeoma ^a   Castro, Maria G ^a   Lowenstein, Pedro R ^a  

^a UNIVERSITY OF MANCHESTER   (United Kingdom)

Author keywords

Acute inflammation; Adenovirus; Chronic inflammation; Dose response; Gene transfer; Vector mediated direct cytotoxicity

Indexed keywords

BETA GALACTOSIDASE; HALOTHANE; HYPOXANTHINE PHOSPHORIBOSYLTRANSFERASE; VIRUS VECTOR;

ADENOVIRUS; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; BRAIN; CELL LOSS; CHRONIC DISEASE; CHRONIC INFLAMMATION; CONTROLLED STUDY; CYTOTOXICITY; DISEASE SEVERITY; DOSE RESPONSE; EVALUATION; GENE EXPRESSION; GENETIC TRANSDUCTION; GLIA CELL; IMMUNOCOMPETENCE; IMMUNOHISTOCHEMISTRY; INFLAMMATION; INJECTION; NERVE CELL; NEUROLOGIC DISEASE; NONHUMAN; RAT; TIME; TRANSGENE;

ADENOVIRIDAE; ANIMALIA;

EID: 0034986065     PISSN: 15250016     EISSN: None     Source Type: Journal    
DOI: 10.1006/mthe.2000.0224     Document Type: Article

References (26)

1. Chronic brain inflammation and persistent herpes simplex virus 1 thymidine kinase expression in survivors of syngenic glioma treated by adenovirus-mediated gene therapy: Implications for clinical trials
2. Peripheral infection with adenovirus causes unexpected long-term brain inflammation in animals injected intracranially with first-generation, but not with high-capacity adenovirus vectors: Towards realistic gene therapy for chronic diseases
3. Phase I study of adenoviral delivery of the HSV-tk gene and ganciclovir administration in patients with recurrent malignant brain tumors
4. Adenovirus gene transfer causes inflammation in the brain
5. Interleukin-1 mediates a rapid inflammatory response after injection of adenoviral vectors into the brain
6. Immune responses to reporter proteins and high viral dose limit duration of expression with adenoviral vectors: Comparison of E2a wild type and E2a deleted vectors
7. A model for long-term transgene expression in spinal cord regeneration studies
8. Intracranial administration of adenovirus expressing HSV-YK in combination with ganciclovir produces a dose-dependent, self-limiting inflammatory response
9. Induction of apoptosis and G2/M arrest by infection with replication-deficient adenovirus at high multiplicity of infection
10. Analysis of events leading to neuronal death after infection with E1-deficient adenovirus vectors
11. Central nervous system toxicity of two adenoviral vectors encoding variants of the herpes simplex virus type 1 thymidine kinase: Reduced cytotoxicity of a truncated HSV1-TK
12. Adenoviral vector-mediated gene expression in the nervous system of immunocompetent Wistar and T cell-deficient nude rats: Preferential survival of transduced astroglial cells in nude rats
13. Adenoviral vectors for in vivo gene delivery to oligodendrocytes: Transgene expression and cytopathic consequences
14. Adenovirus-mediated transgene expression in nonhuman primates
15. Suppression of inflammation by dexamethasone prolongs adenoviral-mediated transgene expression in the facial nucleus of the rat
16. Inflammatory responses and their impact on β-galactosidase transgene expression following adenovirus vector delivery to the primate caudate nucleus
17. Cell type-specific expression in brain cell cultures from a short human cytomegalovirus major intermediate early promoter depends on whether it is inserted into herpesvirus or adenovirus vectors
18. Adenoviruses encoding HPRT correct biochemical abnormalities of HPRT-deficient cells and allow their survival in negative selection medium
19. Constitutive and enhanced expression from the CMV major IE promoter in a defective adenovirus vector
20. Gene transfer into neural cells in vitro using adenoviral vectors
21. Evaluation of the concentration and bioactivity of adenovirus vectors for gene therapy
22. Supernatant rescue assay vs. polymerase chain reaction for detection of wild-type adenovirus-contaminating recombinant adenovirus stocks
23. The immunosuppressant FK506 prolongs transgene expression in brain following adenovirus-mediated gene transfer
24. Strong promoters are the key to highly efficient, noninflammatory and noncytotoxic adenoviral-mediated transgene delivery in the brain in vivo
25. Extensive β-glucuronidase activity in murine central nervous system after adenovirus-mediated gene transfer to the brain
26. High doses of a helper-dependent adenoviral vector yield supraphysiological levels of α1-antitrypsin with negligible toxicity