Rac mediates cytoskeletal rearrangements and increased cell motility induced by urokinase-type plasminogen activator receptor binding to vitronectin

Journal of Cell Biology

Volumn 152, Issue 6, 2001, Pages 1145-1157

  Kjøller, Lars ^a   Hall, Alan ^a  

^a UNIVERSITY COLLEGE LONDON   (United Kingdom)

Author keywords

Cell migration; Cytoskeleton; Rho family GTPases; Urokinase type plasminogen activator receptor; Vitronectin

Indexed keywords

ACTIN; RAC PROTEIN; RHO GUANINE NUCLEOTIDE BINDING PROTEIN; UROKINASE RECEPTOR; VITRONECTIN;

ANIMAL CELL; ARTICLE; CANCER; CELL ADHESION; CELL MOTILITY; CELL STRUCTURE; CONTROLLED STUDY; CYTOSKELETON; FIBROBLAST; IMMUNOCYTOCHEMISTRY; NONHUMAN; PRIORITY JOURNAL; RECEPTOR BINDING;

3T3 CELLS; ACTINS; ANIMALS; CELL MOVEMENT; CELL SIZE; CELL SURFACE EXTENSIONS; COS CELLS; CULTURE MEDIA, SERUM-FREE; CYTOSKELETON; HUMANS; MICE; MICROINJECTIONS; MICROSCOPY, FLUORESCENCE; PLASMIDS; PLASMINOGEN ACTIVATORS; PROTEIN BINDING; RAC GTP-BINDING PROTEINS; RECEPTORS, CELL SURFACE; RHO GTP-BINDING PROTEINS; SIGNAL TRANSDUCTION; VINCULIN; VITRONECTIN;

ANIMALIA; MURINAE;

EID: 0035911966     PISSN: 00219525     EISSN: None     Source Type: Journal    
DOI: 10.1083/jcb.152.6.1145     Document Type: Article

References (86)

1. Tumor dormancy induced by downregulation of urokinase receptor in human carcinoma involves integrin and MAPK signaling
2. A role for Cdc42 in macrophage chemotaxis
3. The urokinase-type plasminogen activator system in cancer metastasis: A review
4. The receptor-binding sequence of urokinase. A biological function for the growth-factor module of proteases
5. Motility and invasion are differentially modulated by Rho family GTPases
6. The structure and function of the urokinase receptor, a membrane protein governing plasminogen activation on the cell surface
7. Domain interplay in the urokinase receptor. Requirement for the third domain in high affinity ligand binding and demonstration of ligand contact sites in distinct receptor domains
8. A urokinase receptor-associated protein with specific collagen binding properties
9. The urokinase receptor. A cell surface, regulated chemokine
10. Urokinase plasminogen activator receptor, beta 2-integrins, and Src-kinases within a single receptor complex of human monocytes
11. Induction of cell migration by pro-urokinase binding to its receptor: Possible mechanism for signal transduction in human epithelial cells
12. Identification of two distinct mechanisms of phagocytosis controlled by different Rho GTPases
13. Urokinase receptor interacts with alpha(v)beta5 vitronectin receptor, promoting urokinase-dependent cell migration in breast cancer
14. Role of urokinase receptor and caveolin in regulation of integrin signaling
15. Extracellular-regulated kinase activation and CAS/Crk coupling regulate cell migration and suppress apoptosis during invasion of the extracellular matrix
16. Vitronectin regulates the synthesis and localization of urokinase-type plasminogen activator in HT-1080 cells
17. Binding of high molecular weight kininogen to human endothelial cells is mediated via a site within domains 2 and 3 of the urokinase receptor
18. Src-dependence and pertussis-toxin sensitivity of urokinase receptor-dependent chemotaxis and cytoskeleton reorganization in rat smooth muscle cells
19. Is plasminogen activator inhibitor-1 the molecular switch that governs urokinase receptor-mediated cell adhesion and release?
20. Rac regulates integrin-mediated spreading and increased adhesion of T lymphocytes
21. Characterization of the cellular binding site for the urokinase-type plasminogen activator
22. A urokinase-sensitive region of the human urokinase receptor is responsible for its chemotactic activity
23. Urokinase-dependent angiogenesis in vitro and diacylglycerol production are blocked by antisense oligonucleotides against the urokinase receptor
24. Mapping part of the functional epitope for ligand binding on the receptor for urokinase-type plasminogen activator by site-directed mutagenesis
25. Integrin signaling
26. Glioblastoma expression of vitronectin and the alpha v beta 3 integrin. Adhesion mechanism for transformed glial cells
27. Cerebral microenvironment influences expression of the vitronectin gene in astrocytic tumors
28. The urokinase receptor is required for human monocyte chemotaxis in vitro
29. Urokinase receptor-deficient mice have impaired neutrophil recruitment in response to pulmonary Pseudomonas aeruginosa infection
30. Rho GTPases and the actin cytoskeleton
31. PDGF stimulates an increase in GTP-Rac via activation of phosphoinositide 3-kinase
32. Linkage of extracellular plasminogen activator to the fibroblast cytoskeleton: Colocalization of cell surface urokinase with vinculin
33. The intact urokinase receptor is required for efficient vitronectin binding: Receptor cleavage prevents ligand interaction
34. The urokinase receptor is a major vitronectin-binding protein on endothelial cells
35. Cdc42 and Rac1 induce integrin-mediated cell motility and invasiveness through PI(3)K
36. Proximity oscillations of complement type 4 (alphaX beta2) and urokinase receptors on migrating neutrophils
37. Identification and characterization of genes associated with human hepatocellular carcinogenesis
38. Urokinase receptor is associated with the components of the JAK1/STAT1 signaling pathway and leads to activation of this pathway upon receptor clustering in the human kidney epithelial tumor cell line TCL-598
39. Mechanisms of signaling through urokinase receptor and the cellular response
40. The Ras-related protein Cdc42Hs and bradykinin promote formation of peripheral actin microspikes and filopodia in Swiss 3T3 fibroblasts
41. Urokinase stimulates human vascular smooth muscle cell migration via a phosphatidyl inositol 3-kinase-Tyk2 interaction
42. Cell migration: A physically integrated molecular process
43. Lymphocyte adhesion molecule ligands and extracellular matrix proteins in gliomas and normal brain: Expression of VCAM-1 in gliomas
44. Evidence that SH2 domains promote processive phosphorylation by protein-tyrosine kinases
45. A role for Rac in Tiam1-induced membrane ruffling and invasion
46. Biology and biochemistry of proteinases in tumor invasion
47. Actin-based cell motility and cell locomotion
48. Distribution and lateral mobility of the urokinase-receptor complex at the cell surface
49. Myosin light chain kinase functions downstream of Ras/ERK to promote migration of urokinase-type plasminogen activator-stimulated cells in an integrin-selective manner
50. Rho, rac, and cdc42 GTPases regulate the assembly of multimolecular focal complexes associated with actin stress fibers, lamellipodia, and filopodia
51. Rho GTPases control polarity, protrusion, and adhesion during cell movement
52. Activation of the small GTP-binding proteins rho and rac by growth factor receptors
53. Mannose 6-phosphate/insulin-like growth factor-II receptor targets the urokinase receptor to lysosomes via a novel binding interaction
54. Integrin signalling: A new Cas(t) of characters enters the stage
55. Urokinase receptor and integrin partnership: Coordination of signaling for cell adhesion, migration and growth
56. Influence of stereochemistry of the sequence Arg-Gly-Asp-Xaa on binding specificity in cell adhesion
57. Ligand interaction between urokinase-type plasminogen activator and its receptor probed with 8-anilino-l-naphthalenesulfonate. Evidence for a hydrophobic binding site exposed only on the intact receptor
58. Ultrastructural localization of plasma membrane-associated urokinase-type plasminogen activator at focal contacts
59. Physicochemical characterization of human S-protein and its function in the blood coagulation system
60. Urokinase receptor: A molecular organizer in cellular communication
61. Binding of human urokinase-type plasminogen activator to its receptor: Residues involved in species specificity and binding
62. Proteolytic cleavage of the urokinase receptor substitutes for the agonist-induced chemotactic effect
63. The small GTP-binding protein rho regulates the assembly of focal adhesions and actin stress fibers in response to growth factors
64. The small GTP-binding protein rac regulates growth factor-induced membrane ruffling
65. 2-terminal domain of the urokinase receptor
66. Matrix-dependent Tiam1/Rac signaling in epithelial cells promotes either cell-cell adhesion or cell migration and is regulated by phosphatidylinositol 3-kinase
67. Human p21-activated kinase (Pak1) regulates actin organization in mammalian cells
68. p21-activated kinase 1 (Pak1) regulates cell motility in mammalian fibroblasts
69. Activation of phosphoinositide 3-OH kinase by the alpha6beta4 integrin promotes carcinoma invasion
70. Domain 1 of the urokinase receptor (uPAR) is required for uPAR-mediated cell binding to vitronectin
71. Mac-1 (CD11b/CD18) and the urokinase receptor (CD87) form a functional unit on monocytic cells
72. Identification of a urokinase receptor-integrin interaction site. Promiscuous regulator of integrin function
73. The urokinase receptor (CD87) facilitates CD11b/CD18-mediated adhesion of human monocytes
74. Urokinase receptor (CD87) aggregation triggers phosphoinositide hydrolysis and intracellular calcium mobilization in mononuclear phagocytes
75. Melanoma cell migration on vitronectin: Regulation by components of the plasminogen activation system
76. Invasion of T-lymphoma cells: Cooperation between Rho family GTPases and lysophospholipid receptor signaling
77. Differentiation-enhanced binding of the amino-terminal fragment of human urokinase plasminogen activator to a specific receptor on U937 monocytes
78. Rho GTPases and signaling networks
79. A cellular binding site for the Mr 55,000 form of the human plasminogen activator, urokinase
80. Plasmin and plasminogen activator inhibitor type 1 promote cellular motility by regulating the interaction between the urokinase receptor and vitronectin
81. Identification of the urokinase receptor as an adhesion receptor for vitronectin
82. Regulation of integrin function by the urokinase receptor
83. Localization of urokinase to focal adhesions by human fibro-sarcoma cells synthesizing recombinant vitronectin
84. Role of urokinase and its receptor in basal and stimulated colonic epithelial cell migration in vitro
85. Urokinase-type plasminogen activator receptors associate with betal and beta3 integrins of fibrosarcoma cells: Dependence on extracellular matrix components
86. Urokinase-type plasinogen activator binding to its receptor stimulates tumor cell migration by enhancing integrin-mediated signal transduction