The karyopherin Kap142p/Msn5p mediates nuclear import and nuclear export of different cargo proteins

Journal of Cell Biology

Volumn 152, Issue 4, 2001, Pages 729-739

  Yoshida, Kimihisa ^a   Blobel, Günter ^a  

^a ROCKEFELLER UNIVERSITY   (United States)

Author keywords

Bleomycin; Karyopherin; Nuclear transport; Saccharomyces cerevisiae; Single stranded DNA binding protein

Indexed keywords

BLEOMYCIN; CARRIER PROTEIN; CELL DNA; DNA BINDING PROTEIN; GUANOSINE TRIPHOSPHATE; KARYOPHERIN; REPLICATION PROTEIN A;

ARTICLE; CELL NUCLEUS; CONTROLLED STUDY; DNA RECOMBINATION; DNA REPAIR; DNA REPLICATION; FUNGAL GENE; INTRACELLULAR TRANSPORT; NONHUMAN; PRIORITY JOURNAL; PROTEIN PROTEIN INTERACTION; PROTEIN TRANSPORT; SACCHAROMYCES CEREVISIAE; SIGNAL TRANSDUCTION;

4-NITROQUINOLINE-1-OXIDE; ACTIVE TRANSPORT, CELL NUCLEUS; BLEOMYCIN; CARRIER PROTEINS; CELL NUCLEUS; CYTOPLASM; DNA REPAIR; DNA-BINDING PROTEINS; GENE DELETION; KARYOPHERINS; MICROBIAL SENSITIVITY TESTS; PROTEIN BINDING; RAN GTP-BINDING PROTEIN; RECEPTORS, CYTOPLASMIC AND NUCLEAR; REPLICATION PROTEIN A; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS; TRANSCRIPTION FACTORS;

FUNGI; MYXOGASTRIA; SACCHAROMYCES; SACCHAROMYCES CEREVISIAE;

EID: 0035911152     PISSN: 00219525     EISSN: None     Source Type: Journal    
DOI: 10.1083/jcb.152.4.729     Document Type: Article

References (86)

1. Two novel related yeast nucleoporins Nup170p and Nup157p: Complementation with the vertebrate homologue Nup155p and functional interactions with the yeast nuclear pore-membrane protein Pom152p
2. Kap104p: A karyopherin involved in the nuclear transport of messenger RNA binding proteins
3. Genetic relationships between the G protein βγ complex, Ste5p, Ste20p and Cdc42p: Investigation of effector roles in the yeast pheromone response pathway
4. A novel nuclear import pathway for the transcription factor TFIIS
5. The Saccharomyces cerevisiae RanGTP-binding protein Msn5p is involved in different signal transduction pathways
6. Protein complexes in nucleotide excision repair
7. Stimulation of RTH1 nuclease of the yeast Saccharomyces cerevisiae by replication protein A
8. Nuclear export of Far1p in response to pheromones requires the export receptor Msn5p/Ste21p
9. Yeast replication factor-A functions in the unwinding of the SV40 origin of DNA replication
10. Replication factor-A from Saccharomyces cerevisiae is encoded by three essential genes coordinately expressed at S phase
11. The ATM homologue MEC1 is required for phosphorylation of replication protein A in yeast
12. Sequence and characterization of cytoplasmic nuclear protein import factor p97
13. Structure of the nuclear transport complex karyopherin-β2-Ran x GppNHp
14. Crystallographic analysis of the recognition of a nuclear localization signal by the nuclear import factor karyopherin α
15. The nuclear exportin Msn5 is required for nuclear export of the Mig1 glucose repressor of Saccharomyces cerevisiae
16. Identification of a yeast karyopherin heterodimer that targets import substrate to mammalian nuclear pore complexes
17. Increased dosage of the MSN1 gene restores invertase expression in yeast mutants defective in the SNF1 protein kinase
18. Two homologous zinc finger genes identified by multicopy suppression in a SNF1 protein kinase mutant of Saccharomyces cerevisiae
19. An improved procedure for enzymatic digestion of polyvinylidene difluoride-bound proteins for internal sequence analysis
20. Regulated nucleo/cytoplasmic exchange of HOG1 MAPK requires the importin β homologs NMD5 and XPO1
21. The yeast polyubiquitin gene is essential for resistance to high temperatures, starvation, and other stresses
22. A novel allele of Saccharomyces cerevisiae RFA1 that is deficient in recombination and repair and suppressible by RAD52
23. The nuclear transport factor karyopherin β binds stoichiometrically to Ran-GTP and inhibits the Ran GTPase activating protein
24. Putative reaction intermediates in Crm1-mediated nuclear protein export
25. Disassembly of RanGTP-karyopherin β complex, an intermediate in nuclear protein import
26. Links between replication, recombination and genome instability in eukaryotes
27. The nucleoporin Nup98 is a site for GDP/GTP exchange on Ran and termination of karyopherin β2-mediated nuclear import
28. CRM1 is an export receptor for leucine-rich nuclear export signals
29. Peptide-mass profiles of polyvinylidene difluoride-bound proteins by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry in the presence of nonionic detergents
30. Transport between the cell nucleus and the cytoplasm
31. Isolation of a protein that is essential for the first step of nuclear protein import
32. A novel class of RanGTP binding proteins
33. Partners and pathways repairing a double-strand break
34. Studies of the interaction between Rad52 protein and the yeast single-stranded DNA binding protein RPA
35. Yeast Los1p has properties of an exportin-like nucleocytoplasmic transport factor for tRNA
36. Cse1p is required for export of Srp1p/importin-α from the nucleus in Saccharomyces cerevisiae
37. Replication protein A (RPA): The eukaryotic SSB
38. The nuclear pore-targeting complex binds to nuclear pores after association with a karyophile
39. The asymmetric distribution of the constituents of the Ran system is essential for transport into and out of the nucleus
40. The importin β/importin 7 heterodimer is a functional nuclear import receptor for histone H1
41. Nuclear import of RPA in Xenopus egg extracts requires a novel protein XRIPα but not importin α
42. The receptor Msn5 exports the phosphorylated transcription factor Pho4 out of the nucleus
43. Phosphorylation regulates association of the transcription factor Pho4 with its import receptor Pse1/Kap121
44. Autoinhibition by an internal nuclear localization signal revealed by the crystal structure of mammalian importin α
45. Disruption of six novel yeast genes located on chromosome II reveals one gene essential for vegetative growth and two required for sporulation and conferring hypersensitivity to various chemicals
46. Export of importin α from the nucleus is mediated by a specific nuclear transport factor
47. Saccharomyces Ku70, mre11/rad50 and RPA proteins regulate adaptation to G2/M arrest after DNA damage
48. Replication factor A is required in vivo for DNA replication, repair, and recombination
49. Regulators of pseudohyphal differentiation in Saccharomyces cerevisiae identified through multicopy suppressor analysis in ammonium permease mutant strains
50. Involvement of the Saccharomyces cerevisiae HDF1 gene in DNA double-strand break repair and recombination
51. Nuclear shuttling of yeast scaffold Ste5 is required for its recruitment to the plasma membrane and activation of the mating MAPK cascade
52. Tcn1p/Crz1p, a calcineurin-dependent transcription factor that differentially regulates gene expression in Saccharomyces cerevisiae
53. SUMO-1 modification and its role in targeting the Ran GTPase-activating protein, RanGAP1, to the nuclear pore complex
54. Nuclear import of Cdk/cyclin complexes: Identification of distinct mechanisms for import of Cdk2/cyclin E and Cdc2/cyclin B1
55. Ran and nuclear transport
56. Transport of proteins and RNAs in and out of the nucleus
57. Rad52 protein stimulates DNA strand exchange by Rad5l and replication protein A
58. Nuclear import of U snRNPs requires importin β
59. Importin β can mediate the nuclear import of an arginine-rich nuclear localization signal in the absence of importin α
60. Multiple pathways of recombination induced by double-strand breaks in Saccharomyces cerevisiae
61. A distinct and parallel pathway for the nuclear import of an mRNA-binding protein
62. Transport routes through the nuclear pore complex
63. Nuclear import of the TATA-binding protein: Mediation by the karyopherin Kap114p and a possible mechanism for intranuclear targeting
64. A novel receptor-mediated nuclear protein import pathway
65. Nucleotide excision repair in yeast
66. Protein import into nuclei: Association and dissociation reactions involving transport substrate, transport factors, and nucleoporins
67. A nuclear import pathway for a protein involved in tRNA maturation
68. Nuclear import and the evolution of a multifunctional RNA-binding protein
69. A distinct nuclear import pathway used by ribosomal proteins
70. The yeast nuclear pore complex: Composition, architecture, and transport mechanism
71. Yrb4p, a yeast ran-GTP-binding protein involved in import of ribosomal protein L25 into the nucleus
72. Importin/karyopherin protein family members required for mRNA export from the nucleus
73. Mtr10p functions as a nuclear import receptor for the mRNA-binding protein Np13p
74. The 32- and 14-kilodalton subunits of replication protein A are responsible for species-specific interactions with single-stranded DNA
75. Cse1p is involved in export of yeast importin α from the nucleus
76. Exportin 1 (Crm1p) is an essential nuclear export factor
77. A single-stranded DNA-binding protein is needed for efficient presynaptic complex formation by the Saccharomyces cerevisiae Rad51 protein
78. The nuclear import of RCC1 requires a specific nuclear localization sequence receptor, karyopherin α3/Qip
79. The karyopherin Kap122p/Pdr6p imports both subunits of the transcription factor IIA into the nucleus
80. The arginine-rich domains present in human immunodeficiency virus type 1 Tat and Rev function as direct importin β-dependent nuclear localization signals
81. The DNA replication fork in eukaryotic cells
82. A new family of yeast nuclear pore complex proteins
83. Replication protein A: A heterotrimeric, single-stranded DNA-binding protein required for eukaryotic DNA metabolism
84. Purification and characterization of replication protein A, a cellular protein required for in vitro replication of simian virus 40 DNA
85. Karyopherins and kissing cousins
86. GTP hydrolysis links initiation and termination of nuclear import on the nucleoporin nup358