A nuclear import pathway for a protein involved in tRNA maturation

Journal of Cell Biology

Volumn 139, Issue 7, 1997, Pages 1655-1661

  Rosenblum, Jonathan S ^a   Pemberton, Lucy F ^a   Blobel, Günter ^a  

^a ROCKEFELLER UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AUTOANTIGEN; RIBOSOME PROTEIN; TRANSFER RNA;

ARTICLE; CELL FRACTIONATION; CELL NUCLEUS; CYTOPLASM; CYTOSOL; NONHUMAN; NORTHERN BLOTTING; PRIORITY JOURNAL; PROTEIN LOCALIZATION; PROTEIN TRANSPORT; RIBOSOME; RNA PROCESSING; SACCHAROMYCES CEREVISIAE;

AMINO ACID SEQUENCE; BETA KARYOPHERINS; CELL NUCLEUS; FUNGAL PROTEINS; GENE DELETION; MOLECULAR SEQUENCE DATA; NUCLEAR ENVELOPE; NUCLEAR PROTEINS; RNA PROCESSING, POST-TRANSCRIPTIONAL; RNA, TRANSFER; RNA-BINDING PROTEINS; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS; SEQUENCE HOMOLOGY, AMINO ACID;

SACCHAROMYCES CEREVISIAE;

EID: 0031426653     PISSN: 00219525     EISSN: None     Source Type: Journal    
DOI: 10.1083/jcb.139.7.1655     Document Type: Article

References (49)

1. Aitchison, J.D., M.P. Rout, M. Marelli, G. Blobel, and R.W. Wozniak. 1995. Two novel related yeast nueleoporins Nup170p and Nup157p: complementation with the vertebrate homologue Nup155p and functional interactions with the yeast nuclear pore-membrane protein Pom152p. J. Cell Biol. 131: 1133-1148.
2. Aitchison, J.D., G. Blobel, and M.P. Rout. 1996. Kap104p: a karyopherin involved in the nuclear transport of messenger RNA binding proteins. Science. 274:624-627.
3. Bachmann, M., K. Pfeifer, H.C. Schroder, and W.E. Muller. 1990. Characterization of the autoantigen La as a nucleic acid-dependent ATPase/dATPase with melting properties. Cell. 60:85-93.
4. Boelens, W.C., I. Palacios, and I.W. Mattaj. 1995. Nuclear retention of RNA as a mechanism for localization. RNA. 1:273-283.
5. Dingwall, C., and R.A. Laskey. 1991. Nuclear targeting sequences - a consensus? Trends Biol. Sci. 16:478-481.
6. Doye, V., and E. Hurt. 1997. From nueleoporins to nuclear pore complexes. Curr. Opin. Cell Biol. 9:401-411.
7. Fernandez, J., L. Andrews, and S.M. Mische. 1994. An improved procedure for enzymatic digestion of polyvinylidene difluoride-bound proteins for internal sequence analysis. Anal. Biochem. 218:112-117.
8. Finley, D., E. Ozkaynak, and A. Varshavsky. 1987. The yeast polyubiquitin gene is essential for resistance to high temperatures, starvation, and other stresses. Cell. 48:1035-1046.
9. Fischer, U., E. Darzynkiewicz, S.M. Tahara, N.A. Dathan, R. Luhrmann, and I.W. Mattaj. 1991. Diversity in the signals required for nuclear accumulation of U snRNPs and variety in the pathways of nuclear transport. J. Cell Biol. 113:705-714.
10. Fornerod, M., J. van Deursen, S. van Baal, A. Reynolds, D. Davis, K. G. Murti, J. Fransen, and G. Grosveld. 1997. The human homologue of yeast CRM1 is in a dynamic subcomplex with CAN/Nup214 and a novel nuclear pore component Nup88. EMBO (Eur. Mol. Biol. Organ.) J. 16:807-816.
11. Garcia-Bustos, J.F., P. Wagner, and M.N. Hall. 1991. Nuclear import substrates compete for a limited number of binding sites. Evidence for different classes of yeast nuclear import receptors. J. Biol. Chem. 266:22303-22306.
12. Gharahdaghi, F., M. Kirchner, J. Fernandez, and S.M. Mische. 1996. Peptide-mass profiles of polyvinylidene difluoride-bound proteins by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry in the presence of nonionic detergents. Anal. Biochem. 233:94-99.
13. Gottlieb, E., and J.A. Steitz. 1989. The RNA binding protein La influences both the accuracy and the efficiency of RNA polymerase III transcription in vitro. EMBO (Eur. Mol. Biol. Organ.) J. 8:841-850.
14. Grimm, C., E. Lund, and J.E. Dahlberg. 1997. In vivo selection of RNAs that localize in the nucleus. EMBO (Eur. Mol. Biol. Organ.) J. 16:793-806.
15. Guddat, U., A.H. Bakken, and T. Pieler. 1990. Protein-mediated nuclear export of RNA: 5S rRNA containing small RNPs in Xenopus oocytes. Cell. 60: 619-628.
16. Haselbeck, R.C., and C.L. Greer. 1993. Minimum intron requirements for tRNA splicing and nuclear transport in Xenopus oocytes. Biochemistry. 32: 8575-8581.
17. Hendrick, J.P., S.L. Wolin, J. Rinke, M.R. Lerner, and J.A. Steitz. 1981. Ro small cytoplasmic ribonucleoproteins are a subclass of La ribonucleoproteins: further characterization of the Ro and La small ribonucleoproteins from uninfected mammalian cells. Mol. Cell. Biol. 1:1138-1149.
18. Kadowaki, T., S. Chen, M. Hitomi, E. Jacobs, C. Kumagai, S. Liang, R. Schneiter, D. Singleton, J. Wisniewska, and A.M. Tartakoff. 1994. Isolation and characterization of Saccharomyces cerevisiae mRNA transport-defective (mtr) mutants. J. Cell Biol. 126:649-659.
19. Kraemer, D.M., C. Strambio-de-Castillia, G. Blobel, and M.P. Rout. 1995. The essential yeast nucleoporin NUP159 is located on the cytoplasmic side of the nuclear pore complex and serves in karyopherin-mediated binding of transport substrate. J. Biol. Chem. 270:19017-19021.
20. Kutay, U., F.R. Bischoff, S. Kostka, R. Kraft, and D. Görlich. 1997. Export of importin α from the nucleus is mediated by a specific nuclear transport factor. Cell. 90:1061-1071.
21. Lin-Marq, N., and S.G. Clarkson. 1995. A yeast RNA binding protein that resembles the human autoantigen La. J. Mol. Biol. 245:81-85.
22. Makkerh, J.P.S., C. Dingwall, and R.A. Laskey. 1996. Comparative mutagenesis of nuclear localization signals reveals the importance of neutral and acidic amino acids. Curr. Biol. 6:1025-1027.
23. Meerovitch, K., Y.V. Svitkin, H.S. Lee, F. Lejbkowicz, D.J. Kenan, E.K. Chan, V.I. Agol, J.D. Keene, and N. Sonenberg. 1993. La autoantigen enhances and corrects aberrant translation of poliovirus RNA in reticulocyte lysate. J. Virol. 67:3798-3807.
24. Michaud, N., and D. Goldfarb. 1992. Microinjected U snRNAs are imported to oocyte nuclei via the nuclear pore complex by three distinguishable targeting pathways. J. Cell Biol. 116:851-861.
25. Michaud, N., and D.S. Goldfarb. 1991. Multiple pathways in nuclear transport: the import of U2 snRNP occurs by a novel kinetic pathway. J. Cell Biol. 112: 215-223.
26. Moritz, M., A.G. Paulovich, Y.-F. Tsay, and J.L. Woolford, Jr. 1990. Depletion of yeast ribosomal proteins L16 or rp59 disrupts ribosome assembly. J. Cell Biol. 111:2261-2274.
27. Moritz, M., B.A. Pulaski, and J.L. Woolford, Jr. 1991. Assembly of 60S ribosomal subunits is perturbed in temperature-sensitive yeast mutants defective in ribosomal protein L16. Mol. Cell Biol. 11:5681-5692.
28. Moroianu, J. 1997. Molecular mechanisms of nuclear protein transport. Crit. Rev. Eukaryotic Gene Expr. 7:61-72.
29. Moroianu, J., G. Blobel, and A. Radu. 1996. The binding site of karyopherin α for karyopherin β overlaps with a nuclear localization sequence. Proc. Natl. Acad. Sci. USA. 93:6572-6576.
30. Moroianu, J., G. Blobel, and A. Radu. 1997. RanGTP-mediated nuclear export of karyopherin α involves its interaction with the nucleoporin Nup153. Proc. Natl. Acad. Sci. USA. 94:9699-9704.
31. Nehrbass, U., and G. Blobel. 1996. Role of the nuclear transport factor p10 in nuclear import. Science. 272:120-122.
32. Nigg, E.A. 1997. Nucleocytoplasmic transport: signals, mechanisms and regulation. Nature. 386:779-787.
33. Rexach, M., and G. Blobel. 1995. Protein import into nuclei: association and dissociation reactions involving transport substrate, transport factors, and nucleoporins. Cell. 83:683-692.
34. Rinke, J., and J.A. Steitz. 1982. Precursor molecules ol both human 5S ribosomal RNA and transfer RNAs are bound by a cellular protein reactive with anti-La lupus antibodies. Cell. 29:149-159.
35. Rinke, J., and J.A. Steitz. 1985. Association of the lupus antigen La with a subset of U6 snRNA molecules. Nucleic Acids Res. 13:2617-2629.
36. Rose, M.D., F. Winston, and P. Hieter. 1990. Methods in Yeast Genetics: A Laboratory Course Manual. Cold Spring Harbor Press, Cold Spring Harbor, NY. 198 pp.
37. Rotenbcrg, M.O., M. Moritz, and J.L. Woolford, Jr. 1988. Depletion of Saccharomyces cerevisiae ribosomal protein L16 causes a decrease in 60S ribosomal subunits and formation of half-mer polyribosomes. Genes Dev. 2:160-172.
38. Rout, M.P., G. Blobel, and J.D. Aitchison. 1997. A distinct nuclear import pathway used by ribosomal proteins. Cell. 89:715-725.
39. Seedorf, M. and P.A. Silver. 1997. Importin/karyopherin protein family members required for mRNA export from the nucleus. Proc. Natl. Acad. Sci. USA. 94:8590-8595.
40. Shulga, N., P. Roberts, Z. Gu, L. Spitz, M.M. Tabb, M. Nomura, and D.S. Goldfarb. 1996. In vivo nuclear transport kinetics in Saccharomyces cerevisiae: a role for heal shock protein 70 during targeting and translocation. J. Cell Biol. 135:329-339.
41. Simons, F.H., F.J. Broers, W.J. Van Venrooij, and G.J. Pruijn. 1996. Characterization of cis-acting signals for nuclear import and retention of the La (SS-B) autoantigen. Exp. Cell Res. 224:224-236.
42. Stade, K., C.S. Ford, C. Guthrie, and K. Weis. 1997. Exportin 1 (Crm1) is an essential nuclear export factor. Cell. 90:1041-1050.
43. Stefano, J.E. 1984. Purified lupus antigen La recognizes an oligouridylate stretch common to the 3′ termini of RNA polymerase III transcripts. Cell. 36:145-154.
44. Tarn, W.-Y., T.A. Yario, and J.A. Steitz. 1995. U12 snRNA in vertebrates: evolutionary conservation of 5′ sequences implicated in splicing of pre-mRNAs containing a minor class of intron. RNA. 1:644-656.
45. Ullman, K.S., M.A. Powers, and D.J. Forbes. 1997. Nuclear export receptors: from importin to exportin. Cell. 90:967-970.
46. Yaffe, M.P., and G. Schatz. 1984. Two nuclear mutations that block mitochondrial protein import. Proc. Natl. Acad. Sci. USA. 81:4819-4823.
47. Yoo, C.J., and S.L. Wolin. 1994. La proteins from Drosophila melanogaster and Saccharomyces cerevisiae: a yeast homolog of the La autoantigen is dispensable for growth. Mol. Cell Biol. 14:5412-5424.
48. Yoo, C.J., and S.L. Wolin. 1997. The yeast La protein is required tor the 3′ endonucleolytic cleavage that matures tRNA precursors. Cell. 89:393-402.
49. Zasloff, M. 1983. tRNA transport from the nucleus in a eukaryotic cell: carrier-mediated translocation process. Proc. Natl. Acad. Sci. USA. 80:6436-6440.