The arginine-rich domains present in human immunodeficiency virus type 1 Tat and Rev function as direct importin β-dependent nuclear localization signals

Molecular and Cellular Biology

Volumn 19, Issue 2, 1999, Pages 1210-1217

  Truant, Ray ^a   Cullen, Bryan R ^a  

^a DUKE UNIVERSITY MEDICAL CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

KARYOPHERIN;

AFFINITY CHROMATOGRAPHY; AMINO ACID SEQUENCE; ARTICLE; BINDING AFFINITY; CYTOPLASM; HELA CELL; HUMAN; HUMAN CELL; HUMAN IMMUNODEFICIENCY VIRUS 1; PLASMID; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN PURIFICATION; SPECIES DIFFERENTIATION;

HUMAN IMMUNODEFICIENCY VIRUS; HUMAN IMMUNODEFICIENCY VIRUS 1;

EID: 0032911885     PISSN: 02707306     EISSN: None     Source Type: Journal    
DOI: 10.1128/mcb.19.2.1210     Document Type: Article

References (47)

1. Adam, S. A., R. E. Sterne-Marr, and L. Gerace. 1990. Nuclear protein import in permeabilized mammalian cells requires soluble cytoplasmic factors. J. Cell Biol. 111:807-816.
2. Bischof, F. R., C. Klebe, J. Kretschmer, A. Wittinghofer, and H. Ponstingl. 1994. RanGAP1 induces GTPase activity of nuclear ras-related Ran. Proc. Natl. Acad. Sci. USA 91:2587-2591.
3. Böhnlein, E., J. Berger, and J. Hauber. 1991. Functional mapping of the human immunodeficiency virus type 1 Rev RNA binding domain: new insights into the domain structure of Rev and Rex. J Virol. 65:7051-7055.
4. Bonifaci, N., J. Moroianu, A. Radu, and G. Blobel. 1997. Karyopherin β2 mediates nuclear import of a mRNA binding protein. Proc. Natl. Acad. Sci. USA 94:5055-5060.
5. Chi, N. C., E. J. H. Adam, and S. A. Adam. 1995. Sequence and characterization of cytoplasmic nuclear protein import factor p97. J. Cell Biol. 130: 265-274.
6. Cochrane, A. W., A. Perkins, and C. A. Rosen. 1990. Identification of sequences important in the nucleolar localization of human immunodeficiency virus Rev: relevance of nucleolar localization to function. J. Virol. 64:881-885.
7. Conti, E., M. Uy, L. Leighton, G. Blobel, and J. Kuriyan. 1998. Crystallographic analysis of the recognition of a nuclear localization signal by the nuclear import factor karyopherin α. Cell 94:193-204.
8. Cullen, B. R. 1998. HIV-1 auxiliary proteins: making connections in a dying cell. Cell 93:685-692.
9. Dingwall, C., S. V. Sharnick, and R. A. Laskey. 1982. A polypeptide domain that specifies migration of nucleoplasmin into the nucleus. Cell 30:449-458.
10. Efthymiadis, A., L. J. Briggs, and D. A. Jans. 1998. The HIV-1 Tat nuclear localization sequence confers novel nuclear import properties. J. Biol. Chem. 273:1623-1628.
11. Emerman, M., and M. H. Malim. 1998. HIV-1 regulatory/accessory genes: keys to unraveling viral and host cell biology. Science 280:1880-1884.
12. Fankhauser, C., E. Izaurralde, Y. Adachi, P. Wingfield, and U. K. Laemmli. 1991. Specific complex of human immunodeficiency virus type 1 Rev and nucleolar B23 proteins: dissociation by the Rev response element. Mol. Cell. Biol. 11:2567-2575.
13. Fridell, R. A., R. Truant, L. Thorne, R. E. Benson, and B. R. Cullen. 1997. Nuclear import of hnRNP A1 is mediated by a novel cellular cofactor related to karyopherin-β. J. Cell Sci. 110:1325-1331.
14. Görlich, D., P. Henklein, R. A. Laskey, and E. Hartmann. 1996. A 41 amino acid motif in importin-α confers binding to importin-β and hence transit into the nucleus. EMBO J. 15:1810-1817.
15. Görlich, D., N. Panté, U. Kutay, U. Aebi, and F. R. Bischoff. 1996. Identification of different roles for RanGDP and RanGTP in nuclear protein import. EMBO J. 15:5584-5594.
16. Görlich, D., F. Vogel, A. D. Mills, E. Hartmann, and R. A. Laskey. 1995. Distinct functions for the two importin subunits in nuclear protein import. Nature 377:246-248.
17. Hammerschmid, M., D. Palmeri, M. Ruhl, H. Jaksche, I. Weichselbraun, E. Böhnlein, M. H. Malim, and J. Hauber. 1994. Scanning mutagenesis of the arginine-rich region of the human immunodeficiency virus type 1 Rev transactivator. J. Virol. 68:7329-7335.
18. Hauber, J., M. H. Malim, and B. R. Cullen. 1989. Mutational analysis of the conserved basic domain of human immunodeficiency virus Tat protein. J. Virol. 63:1181-1187.
19. Heaphy, S., C. Dingwall, I. Ernberg, M. J. Gait, S. M. Green, J. Karn, A. D. Lowe, M. Singh, and M. A. Skinner. 1990. HIV-1 regulator of virion expression (Rev) protein binds to an RNA stem-loop structure located within the Rev response element region. Cell 60:685-693.
20. Henderson, B. R., and P. Percipalle. 1997. Interactions between HIV Rev and nuclear import and export factors: the Rev nuclear localisation signal mediates specific binding to human importin-β. J. Mol. Biol. 274:693-707.
21. Izaurralde, E., U. Kutay, C. von Kobbe, I. W. Mattaj, and D. Görlich. 1997. The asymmetric distribution of the constituents of the Ran system is essential for transport into and out of the nucleus. EMBO J. 16:6535-6547.
22. Jäkel, S., and D. Görlich. 1998. Importin β, transportin, RanBP5 and RanBP7 mediate nuclear import of ribosomal proteins in mammalian cells. EMBO J. 17:4491-4502.
23. Kalderon, D., B. L. Roberts, W. D. Richardson, and A. E. Smith. 1984. A short amino acid sequence able to specify nuclear location. Cell 39:499-509.
24. Kjems, J., M. Brown, D. D. Chang, and P. A. Sharp. 1991. Structural analysis of the interaction between the human immunodeficiency virus Rev protein and the Rev response element. Proc. Natl. Acad. Sci. USA 88:683-687.
25. Koepp, D. M., and P. A. Silver. 1996. A GTPase controlling nuclear trafficking: running the right way or walking RANdomly? Cell 87:1-4.
26. Köhler, M., S. Ansieau, S. Prehn, A. Leutz, H. Haller, and E. Hartmann. 1997. Cloning of two novel human importin-α subunits and analysis of the expression pattern of the importin-α protein family. FEBS Lett. 417:104-108.
27. Malim, M. H., S. Böhnlein, J. Hauber, and B. R. Cullen. 1989. Functional dissection of the HIV-1 Rev transactivator: derivation of a transdominant repressor of Rev function. Cell 58:205-214.
28. Malim, M. H., and B. R. Cullen. 1991 HIV-1 structural gene expression requires the binding of multiple Rev monomers to the viral RRE: implications for HIV-1 latency. Cell 65:241-248.
29. Mattaj, I. W., and L. Englmeier. 1998. Nucleocytoplasmic transport: the soluble phase. Annu. Rev. Biochem. 167:256-306.
30. Meyer, B. E., and M. H. Malim. 1994. The HIV-1 Rev transactivator shuttles between the nucleus and the cytoplasm. Genes Dev. 8:1538-1547.
31. Moore, M. S., and G. Blobel. 1993. The GTP-binding protein Ran/TC4 is required for protein import into the nucleus. Nature 365:661-663.
32. Moroianu, J., G. Blobel, and A. Radu. 1995. Previously identified protein of uncertain function is karyopherin α and together with karyopherin β docks import substrate at nuclear pore complexes. Proc. Natl. Acad. Sci. USA 92:2008-2011.
33. Moroianu, J., G. Blobel, and A. Radu. 1996. Nuclear protein import: Ran-GTP dissociates the karyopherin αβ heterodimer by displacing α from an overlapping binding site on β. Proc. Natl. Acad. Sci. USA 93:7059-7062.
34. Nadler, S. G., D. Tritschler, O. K. Haffar, J. Blake, A. G. Bruce, and J. S. Cleaveland. 1997. Differential expression and sequence-specific interaction of karyopherin α with nuclear localization sequences. J. Biol. Chem. 272: 4310-4315.
35. Palmeri, D., and M. H. Malim. 1999. Importin β can mediate the nuclear import of an arginine-rich nuclear localization signal in the absence of importin α. Mol. Cell. Biol. 19:1218-1225.
36. Paschal, B. M., C. Delphin, and L. Gerace. 1996. Nucleotide-specific interaction of Ran/TC4 with nuclear transport factors NTF2 and p97. Proc. Natl. Acad. Sci. USA 93:7679-7683.
37. Pollard, V. W., W. M. Michael, S. Nakielny, M. C. Siomi, F. Wang, and G. Dreyfuss. 1996. A novel receptor-mediated nuclear protein import pathway. Cell 86:985-994.
38. Radu, A., G. Blobel, and M. S. Moore. 1995. Identification of a protein complex that is required for nuclear protein import and mediates docking of import substrate to distinct nucleoporins. Proc. Natl. Acad. Sci. USA 92: 1769-1773.
39. Rexach, M., and G. Blobel. 1995. Protein import into nuclei: association and dissociation reactions involving transport substrate, transport factors, and nucleoporins. Cell 83:683-692.
40. Siomi, H., H. Shida, M. Maki, and M. Hatanaka. 1990. Effects of a highly basic region of human immunodeficiency virus Tat protein on nucleolar localization. J. Virol. 64:1803-1807.
41. Tiganis, T., A. J. Flint, S. A. Adam, and N. K. Tonks. 1997. Association of the T-cell protein tyrosine phosphatase with nuclear import factor p97. J. Biol. Chem. 272:21548-21557.
42. + RNA binding protein. Mol. Cell. Biol. 18:1449-1458.
43. Tsuji, L., T. Takumi, N. Imamoto, and Y. Yoneda. 1997. Identification of novel homologues of mouse importin α, the α subunit of the nuclear poretargeting complex, and their tissue-specific expression. FEBS Lett. 416:30-34.
44. Weis, K. 1998. Importins and exportins: how to get in and out of the nucleus. Trends Biochem. Sci. 23:185-189.
45. Weis, K., C. Dingwall, and A. I. Lamond. 1996. Characterization of the nuclear protein import mechanism using Ran mutants with altered nucleotide binding specificities. EMBO J. 15:7120-7128.
46. Weis, K., U. Ryder, and A. I. Lamond. 1996. The conserved amino-terminal domain of hSRP1α is essential for nuclear protein import. EMBO J. 15: 1818-1825.
47. Zapp, M. L., and M. R. Green. 1989. Sequence-specific RNA binding by the HIV-1 Rev protein. Nature 342:714-716.