Characterization of purified recombinant Bet v 1 with authentic N- terminus, cloned in fusion with maltose-binding protein

Protein Expression and Purification

Volumn 8, Issue 3, 1996, Pages 365-373

  Spangfort, Michael D ^b   Ipsen, Henrik ^b   Sparholt, Susanne H ^b   Aasmul Olsen, Stig ^b   Larsen, Martin R ^a   Mørtz, Ejvind ^a   Roepstorff, Peter ^a   Larsen, Jørgen N ^b  

^a UNIVERSITY OF SOUTHERN DENMARK   (Denmark)

^b ALK ABELLÓ   (Denmark)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 0030294806     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1006/prep.1996.0112     Document Type: Article

References (33)

1. 1. D'Amato, G., Spieksma, F. T. M., and Bonini, S. (1991) in "Allergenic Pollen and Pollinosis in Europe," Blackwell, Oxford.
2. 2. Belin, L. (1972) Separation and characterization of Birch pollen antigens with special reference to the allergenic components. Int. Arch. Allergy 42, 329-342.
3. 3. Viander, M., Fraki, J., Djupsund, B. M., and Laine, S. (1979) Antigens and allergens in Birch pollen extract. Allergy 34, 289-302.
4. 4. Apold, J., Florvaag, E., and Elsayed, S. (1981) Comparative studies on tree-pollen allergens. Int. Arch. Allergy Appl. Immunol. 64, 439-447.
5. 5. Ipsen, H., and Løwenstein, H. (1983) Isolation and immunochemical characterization of the major allergen of Birch pollen (Betula verrucosa.) J. Allergy Clin. Immunol. 72, 150-159.
6. 2-terminal amino acid sequence of the immunochemically partial identical major allergens of alder (Alnus glutinosa) Aln g I, birch (Betula verrucosa) Bet v I, hornbeam (Carpinus betulus) Car b I and Oak (Quercus alba) Que a I pollens. Mol. Immunol. 28, 1279-1288.
7. 7. Ipsen, H., Wihl, J-Å., Nüchel Petersen, B., and Løwenstein, H. (1992) Specificity mapping of patients IgE response towards the tree pollen allergens Aln g I, Bet v I and Cor a I. Clin. Exp. Allergy 22, 391-399.
8. 8. Breiteneder, H., Pettenburger, K., Bito, A., Valenta, R., Kraft, D., Rumpold, H., Scheiner, O., and Breitenbach, M. (1989) The gene coding for the major birch pollen allergen Bet v I, is highly homologous to a pea disease resistance response gene. EMBO J. 8, 1935-1938.
9. 9. Larsen, J. N., Strøman, P., and Ipsen, H. (1992) PCR based cloning and sequencing of genes encoding the tree pollen major allergen Car b 1 from Carpinus betulus (hornbeam). Mol. Immunol. 29, 703-711.
10. 10. Breiteneder, H., Ferreira, F., Reikerstorfer, A., Duchene, M., Valenta, R., Hoffmann-Sommergruber, K., Ebner, C., Breitenbach, M., Kraft, D., and Scheiner, O. (1992) Complementary DNA cloning and expression in Escherichia coli of Aln g 1, the major allergen in pollen of alder (Alnus glutinosa). J. Allergy Clin. Immunol. 90, 909-917.
11. 11. Breiteneder, H., Ferreira, F., Hoffmann-Sommergruber, K., Ebner, C., Breitenbach, M., Rumpold, H., Kraft, D., and Scheiner, O. (1993) Four recombinant isoallergens of Cor a I, the major allergen of hazel pollen, show different IgE-binding properties. Eur. J. Biochem. 212, 355-362.
12. 12. Ebner, C., Schenk, S., Szepfalusi, Z., Hoffmann, K., Ferreira, F., Willheim, M., Scheiner, O., and Kraft, D. (1993) Multiple T cell specificities for Bet v I, the major birch pollen allergen, within single individuals. Studies using specific T cell clones and overlapping peptides. Eur. J. Immunol. 23, 1523-1527.
13. 13. Walter, M. H., Liu, J-W., Grand, C., Lamb, C. J., and Hess, D. (1990) Bean pathogenesis-related (PR) proteins deduced from elicitor-induced transcripts are members of a ubiquitous new class of conserved PR proteins including pollen allergens. Mol. Gen. Genet. 222, 353-360.
14. 14. Bufe, A., Spangfort, M. D., Kahlert, H., Schlaak, M., and Becker, W-H. (1996) The major birch pollen allergen Bet v 1 shows ribonuclease activity. Planta 199, 413-415.
15. 15. Florvaag, E., Holen, E., Vik, H., and Elsayed, S. (1988) Comparative studies on tree pollen allergens. XIV. Characterization of the birch (Betula verrucosa) and hazel (Corylus avellana) pollen extracts by horizontal 2-D SDS-PAGE combined with electrophoretic transfer and IgE immunoautoradiography. Ann. Allergy 61, 392-400.
16. 16. Jarolim, E., Rumpold, H., Endler, A. T., Ebner, H., Breitenbach, M., Scheiner, O., and Kraft, D. (1989) IgE and IgG antibodies of patients with allergy to birch pollen as tools to define the allergen profile of Betula verrucosa. Allergy 44, 385-395.
17. 17. Løwenstein, H., Sparholt, S. H., Klysner, S. S., Ipsen, H., and Larsen, J. N. (1995) The significance of isoallergenic variations in present and future specific immunotherapy. Int. Arch. Allergy Immunol. 107, 285-289.
18. 18. Larsen, J. N. (1995) Isoallergens, significance in allergen exposure and response. ACI News 7, 141-146.
19. 19. Swoboda, I. (1994) EMBL/GeneBank/DDBJ databases 1994.
20. 20. Larsen, J. N., Casals, A. B., From, N. B., Strøman, P., and Ipsen, H. (1993) Characterization of recombinant Betv 1, the major pollen allergen of Betula verrucosa (White Birch), produced by fedbatch fermentation. Int. Arch. Allergy Clin. Immunol. 102, 249-258.
21. 21. Ferreira, F. D., Hoffmann-Sommergruber, K., Breiteneder, H., Pettenburger, K., Ebner, C., Sommergruber, W., Steiner, R., Bohle, B., Sperr, W. R., Valent, P., Kunl, A. J., Breitenbach, M., Kraft, D., and Scheiner, O. (1993) Purification and characterization of recombinant Bet v 1, the major Birch pollen allergen. J. Biol Chem. 268, 19574-19580.
22. 22. Sidoli, A., Tamborini, E., Giuntin, I., Levi, S., Volonté, G., Paini, C., De Lalla, C., Siccardi, A. G., Baralle, F. E., Galliani, S., and Arosio, P. (1993) Cloning, expression and immunological characterisation of recombinant Lolium perenne allergen Lol p II. J. Biol Chem. 268, 21819-21825.
23. 23. Villalba, M., Batanero, E., Monsalve, R. I., Gonzãlez de la Peña, M. A., Lahoz, C., and Rodriguez, R. (1994) Cloning and expression of Ole e I, the major allergen from Olive tree pollen. J. Biol. Chem. 269, 15217-15222.
24. 24. Kuo, M-C., Zhu, X-J., Koury, R., Griffith, I. J., Klapper, D. G., Bond, J. F., and Rogers, B. L. (1993) Purification and immunochemical characterization of recombinant and native ragweed allergen Amb a II. Mol. Immunol. 30, 1077-1087.
25. 25. Nüchel Petersen, B., Janniche, H., Munch, E. P., Wihl, J-Å., Böwadt, H., Ipsen, H., and Løwenstein, H. (1988) Immunotherapy with partially purified and standardized tree pollen extracts. I. Clinical results from a three-year double-blind study of patients treated with pollen extracts either of birch or combinations of alder, birch and hazel. Allergy 43, 353-362.
26. 26. Maniatis, T., Fritsch, E. F., and Sambrook, J. (1982) in "Molecular Cloning: A Laboratory Manual," Cold Spring Harbor Press, New York.
27. 27. Ausubel, F. M., Brent, R., Kingston, R. E., Moore, D. D., Seidman, J. G., Smith, J. A., and Struhl, K. (1987) "Current Protocols in Molecular Biology," Wiley, New York.
28. 28. Amann, E., and Brosius, J. (1985) 'ATG vectors' for regulated high-level expression of cloned genes in Escherichia coli. Gene 40, 183-190.
29. 29. Hanahan, D., and Meselson, M. (1983) Plasmid screening at high colony density. Methods Enzymol. 100, 333-342.
30. 30. Sanger, F., Nicklen, S., and Coulson, A. R. (1977) DNA sequencing with chain-termination inhibitors. Proc. Natl. Acad. Sci. USA 74, 5463-5467.
31. 31. O'Hehir, R. E., Askonas, B. A., and Lamb, J. R. (1993) Cell culture lymphocyte clones, in "Methods of Immunological Analysis," Vol 3 (Albert, W. H. W., and Staines, N. A., Eds.), pp. 120-138, Springer, Heidelberg.
32. 32. Ipsen, H., and Larsen, J. N. (1988) Detection of antigen specific IgE antibodies in sera from allergic patients by SDS-PAGE immunoblotting and crossed radioimmunoelectrophoresis, in "Handbook of Immunoblotting of Proteins," Vol II (Bjerrum, O., and Heegard, N. H. H., Eds.), pp. 159-166, CRC Press, Boca Raton.
33. 2-terminal sequences of the Group V allergens from Lolium perenne, Poa pratensis and Dactylis glomerata. Clin. Exp. Allergy 22, 491-497.