The chicken B cell line DT40: A novel tool for gene disruption experiments

Journal of Immunological Methods

Volumn 249, Issue 1-2, 2001, Pages 1-16

  Winding, Pernille ^a   Berchtold, Martin W ^a  

^a INSTITUTE OF MOLECULAR BIOLOGY   (Armenia)

Author keywords

Apoptosis; B cell signaling; DT40 chicken B cell line; Gene targeting; Homologous recombination; Ig rearrangements

Indexed keywords

HISTONE; IMMUNOGLOBULIN; LYMPHOCYTE ANTIGEN RECEPTOR; MESSENGER RNA; PROTEIN TYROSINE KINASE; PROTEIN TYROSINE PHOSPHATASE;

APOPTOSIS; B LYMPHOCYTE; CELL SPECIFICITY; CHICKEN; CHROMATIN; CHROMOSOME; CROSS LINKING; DNA REPAIR; GENE DELETION; GENE DISRUPTION; GENE FUNCTION; GENE TARGETING; NONHUMAN; PRIORITY JOURNAL; PROTEIN MODIFICATION; RECOMBINANT DNA TECHNOLOGY; REVIEW;

ANIMALS; CELL LINE; CHICKENS; GENE EXPRESSION PROFILING; HUMANS; RECOMBINATION, GENETIC; SEQUENCE DELETION;

GALLUS GALLUS; MAMMALIA;

EID: 0035282862     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0022-1759(00)00333-1     Document Type: Review

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4. Bruton's tyrosine kinase links the B cell receptor to nuclear factor kappaB activation
5. SYK is upstream of phosphoinositide 3-kinase in B cell receptor signaling
6. Reduced X-ray resistance and homologous recombination frequencies in a RAD54-/- mutant of the chicken DT40 cell line
7. SHIP modulates immune receptor responses by regulating membrane association of Btk
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11. Evidence for an IgD homologue on chicken lymphocytes
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13. Syk and Bruton's tyrosine kinase are required for B cell antigen receptor-mediated activation of the kinase Akt
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19. Efficient conditional mutation of the vertebrate CENP-C gene
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21. CENP-C is necessary but not sufficient to induce formation of a functional centromere
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32. Ongoing diversification of the rearranged immunoglobulin light-chain gene in a bursal lymphoma cell line
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35. Distinct tyrosine phosphorylation sites in ZAP-70 mediate activation and negative regulation of antigen receptor function
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37. Rapid and efficient retrovirus-mediated gene transfer into B cell lines
38. Electromagnetic field-induced stimulation of Bruton's tyrosine kinase
39. Characterisation of calcium signalling in DT40 chicken B-cells
40. Annexin 5 mediates a peroxide-induced Ca(2+) influx in B cells
41. Efficient modification of a human chromosome by telomere-directed truncation in high homologous recombination-proficient chicken DT40 cells
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45. Transphosphorylation of Bruton's tyrosine kinase on tyrosine 551 is critical for B cell antigen receptor function
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48. Use of gene knockouts in cultured cells to study apoptosis
49. Elimination of cyclin D1 in vertebrate cells leads to an altered cell cycle phenotype, which is rescued by overexpression of murine cyclins D1, D2, or D3 but not by a mutant cyclin D1
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51. The chicken HMG-17 gene is dispensable for cell growth in vitro
52. Syk is required for BCR-mediated activation of p90Rsk, but not p70S6k, via a mitogen-activated protein kinase-independent pathway in B cells
53. Neither HMG-14a nor HMG-17 gene function is required for growth of chicken DT40 cells or maintenance of DNaseI-hypersensitive sites
54. The tyrosine kinases Syk and Lyn exert opposing effects on the activation of protein kinase Akt/PKB in B lymphocytes
55. Requirement of SH2-containing protein tyrosine phosphatases SHP-1 and SHP-2 for paired immunoglobulin-like receptor B (PIR-B)-mediated inhibitory signal
56. Paired immunoglobulin-like receptor B (PIR-B) inhibits BCR-induced activation of Syk and Btk by SHP-1
57. Protein tyrosine kinase lyn mediates apoptosis induced by topoisomerase II inhibitors in DT40 cells
58. Avian B-cell development: Generation of an immunoglobulin repertoire by gene conversion
59. Bruton's tyrosine kinase activity and inositol 1,4,5-trisphosphate production are not altered in DT40 lymphoma B cells exposed to power line frequency magnetic fields
60. Generation of an approximately 2.4 Mb human X centromere-based minichromosome by targeted telomere-associated chromosome fragmentation in DT40
61. 2+ signals by differential expression of IP3 receptor subtypes
62. Deposition of genetically engineered human antibodies into the egg yolk of hens
63. The essential functions of human Rad51 are independent of ATP hydrolysis
64. The controlling role of ATM in homologous recombinational repair of DNA damage
65. Genetic depletion reveals an essential role for an SR protein splicing factor in vertebrate cells
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67. Codon usage tabulated from international DNA sequence databases: Status for the year 2000
68. 2+ oscillatory response
69. Deletion of SHIP or SHP-1 reveals two distinct pathways for inhibitory signaling
70. Bruton's tyrosine kinase is required for activation of IkappaB kinase and nuclear factor kappaB in response to B cell receptor engagement
71. B Cell antigen receptor-induced activation of Akt promotes B cell survival and is dependent on Syk kinase
72. Cooperation of tyrosine kinases p72syk and p53/56lyn regulates calcium mobilization in chicken B cell oxidant stress signaling
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74. Distinctive functions of Syk and Lyn in mediating osmotic stress- and ultraviolet C irradiation-induced apoptosis in chicken B cells
75. A deficiency in Syk enhances ceramide-induced apoptosis in DT40 lymphoma B cells
76. Differential regulation of oxidative and osmotic stress induced Syk activation by both autophosphorylation and SH2 domains
77. Targeted disruption of 01H1 encoding a particular H1 histone variant causes changes in protein patterns in the DT40 chicken B cell line
78. Protein kinase C mu (PKC mu) associates with the B cell antigen receptor complex and regulates lymphocyte signaling
79. Parameters of the chicken genome (Gallus gallus)
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81. Sister chromatid exchanges are mediated by homologous recombination in vertebrate cells
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85. One allele of the major histone gene cluster is enough for cell proliferation of the DT40 chicken B cell line
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87. N-Terminal region, C-terminal region, nuclear export signal, and deacetylation activity of histone deacetylase-3 are essential for the viability of the DT40 chicken B cell line
88. Targeted disruption of an H3-IV/H3-V gene pair causes increased expression of the remaining H3 genes in the chicken DT40 cell line
89. Targeted disruption of H2B-V encoding a particular H2B histone variant causes changes in protein patterns on two-dimensional polyacrylamide gel electrophoresis in the DT40 chicken B cell line
90. An approximately half set of histone genes is enough for cell proliferation and a lack of several histone variants causes protein pattern changes in the DT40 chicken B cell line
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92. Histone H1 variants play individual roles in transcription regulation in the DT40 chicken B cell line
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106. Targeted disruption of an essential vertebrate gene: ASF/SF2 is required for cell viability
107. Genetic analysis of the SR protein ASF/SF2: Interchangeability of RS domains and negative control of splicing
108. Possible association of BLM in decreasing DNA double strand breaks during DNA replication
109. Homologous recombination, but not DNA repair, is reduced in vertebrate cells deficient in RAD52
110. Mre11 is essential for the maintenance of chromosomal DNA in vertebrate cells
111. CD45 modulates phosphorylation of both autophosphorylation and negative regulatory tyrosines of Lyn in B cells
112. Syk and Lyn are involved in radiation-induced signaling, but inactivation of Syk or Lyn alone is not sufficient to prevent radiation-induced apoptosis
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114. Cbl suppresses B cell receptor-mediated phospholipase C (PLC)-gamma2 activation by regulating B cell linker protein-PLC-gamma2 binding
115. Deregulation of poly(A) polymerase interferes with cell growth