Functional regions of human telomerase reverse transcriptase and human telomerase RNA required for telomerase activity and RNA-protein interactions

Molecular and Cellular Biology

Volumn 21, Issue 5, 2001, Pages 1888-1897

  Bach, F ^a   Autexier, C ^a  

^a MCGILL UNIVERSITY   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

RNA DIRECTED DNA POLYMERASE; TELOMERASE;

AMINO ACID ANALYSIS; ARTICLE; CARBOXY TERMINAL SEQUENCE; ENZYME ACTIVITY; HUMAN; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN EXPRESSION; PROTEIN INTERACTION; PROTEIN RNA BINDING; RABBIT; RETICULOCYTE; SEQUENCE ANALYSIS;

AMINO ACID MOTIFS; ANIMALS; BLOTTING, NORTHERN; CHROMOSOMES; DNA-BINDING PROTEINS; HUMANS; MODELS, BIOLOGICAL; NUCLEOTIDES; PLASMIDS; PRECIPITIN TESTS; PROTEIN BINDING; PROTEIN BIOSYNTHESIS; PROTEIN STRUCTURE, TERTIARY; RABBITS; RECOMBINANT PROTEINS; RETICULOCYTES; RNA; TELOMERASE; TIME FACTORS; TRANSCRIPTION, GENETIC;

ORYCTOLAGUS CUNICULUS;

EID: 0035137710     PISSN: 02707306     EISSN: None     Source Type: Journal    
DOI: 10.1128/MCB.21.5.1888-1897.2001     Document Type: Article

References (59)

1. A critical role for telomeres in suppressing and facilitating carcinogenesis
2. Telomerase as a possible target for anticancer therapy
3. Boundary elements of the Tetrahymena telomerase RNA template and alignment domains
4. Mutational analysis of the Tetrahymena telomerase RNA: Identification of residues affecting telomerase activity in vitro
5. Reconstitution of human telomerase activity and identification of a minimal functional region of the human telomerase RNA
6. Tetrahymena telomerase ribonucleoprotein RNA-protein interactions
7. Functional reconstitution of human telomerase expressed in Saccharomyces cerevisiae
8. Expression of hTERT and hTR in cis reconstitutes an active human telomerase ribonucleoprotein
9. Reconstitution of human telomerase activity in vitro
10. A functional telomerase RNA swap in vivo reveals the importance of nontemplate RNA domains
11. A mutant of Tetrahymena telomerase reverse transcriptase with increased processivity
12. Telomerase RNA bound by protein motifs specific to telomerase reverse transcriptase
13. Telomerase reverse transcriptase genes identified in Tetrahymena thermophila and Oxytricha trifallax
14. Secondary structure of vertebrate telomerase RNA
15. Mammalian telomeres and telomerase
16. The catalytic subunit of yeast telomerase
17. The human telomerase RNA component
18. Disruption of the telomerase catalytic subunit gene from Arabidopsis inactivates telomerase and leads to a slow loss of telomeric DNA
19. Essential functions of amino-terminal domains in the yeast telomerase catalytic subunit revealed by selection for viable mutants
20. The telomerase RNA pseudoknot is critical for the stable assembly of a catalytically active ribonucleoprotein
21. Expression of mouse telomerase reverse transcriptase during development, differentiation and proliferation
22. Identification of a specific telomere terminal transferase activity in Tetrahymena extracts
23. A telomeric sequence in the RNA of Tetrahymena telomerase required for telomere repeat synthesis
24. Analysis of telomerase catalytic subunit mutants in vivo and in vitro in Schizosaccharomyces pombe
25. Inhibition of telomerase limits the growth of human cancer cells
26. Human telomerase contains evolutionarily conserved catalytic and structural subunits
27. Humanizing the yeast telomerase template
28. Inhibition of human telomerase in immortal human cells leads to progressive telomere shortening and cell death
29. The mouse telomerase RNA 5′-end lies just upstream of the telomerase template sequence
30. Isolation of a candidate human telomerase catalytic subunit gene, which reveals complex splicing patterns in different cell types
31. Telomerase RNA function in recombinant Tetrahymena telomerase
32. Telomerase and chromosome end maintenance
33. Telomerase and DNA end replication: Not a lagging strand problem?
34. Telomerase RNAs of different ciliates have a common secondary structure and a permuted template
35. Reverse transcriptase motifs in the catalytic subunit of telomerase
36. Putative telomerase catalytic subunits from Giardia lamblia and Caenorhabditis elegans
37. Expression of mouse telomerase catalytic subunit in embryos and adult tissues
38. Telomerase activity reconstituted in vitro with purified human telomerase reverse transcriptase and human telomerase RNA component
39. hEST2, the putative human telomerase catalytic subunit gene, is up-regulated in tumor cells and during immortalization
40. Template definition by Tetrahymena telomerase reverse transcriptase
41. A box H/ACA small nucleolar RNA-like domain at the human telomerase RNA 3′ end
42. Human telomerase activation requires two independent interactions between telomerase RNA and telomerase reverse transcriptase
43. Telomerase catalytic subunit homologs from fission yeast and human
44. The telomerase reverse transcriptase: Components and regulation
45. Telomerases
46. Telomeres, telomerase, and cancer: Life on the edge of genomic stability
47. A conserved secondary structure for telomerase RNA
48. Specific telomerase RNA residues distant from the template are essential for telomerase function
49. A survey of telomerase activity in human cancer
50. A conserved pseudoknot in telomerase RNA
51. Two inactive fragments of the integral RNA cooperate to assemble active telomerase with the human protein catalytic subunit (hTERT) in vitro
52. Template boundary in a yeast telomerase specified by RNA structure
53. Role of RNA in enzymatic activity of the reverse transcriptase of hepatitis B virus
54. Three telomerases with completely non-telomeric template replacements are catalytically active
55. Reconstitution of human telomerase with the template RNA component hTR and the catalytic protein subunit hTRT
56. Identification of functionally important domains in the N-terminal region of telomerase reverse transcriptase
57. Analysis of the structure of Tetrahymena nuclear RNAs in vivo: Telomerase RNA, self-splicing rRNA and U2 snRNA
58. Telomere shortening and apoptosis in telomerase-inhibited human tumor cells