A suboptimal 5' splice site is a cis-acting determinant of nuclear export of polyomavirus late mRNAs

Molecular and Cellular Biology

Volumn 16, Issue 11, 1996, Pages 6046-6054

  Huang, Yingqun ^a   Carmichael, Gordon G ^a  

^a UNIVERSITY OF CONNECTICUT HEALTH CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CAPSID PROTEIN; MESSENGER RNA;

ANIMAL CELL; ARTICLE; CELL NUCLEUS; MOUSE; NONHUMAN; PHENOTYPE; POINT MUTATION; POLYOMA VIRUS; PRIORITY JOURNAL; RNA SPLICING; VIRION; VIRUS CAPSID;

ANIMALIA; MIRIDAE; MURINE POLYOMAVIRUS; POLYOMAVIRUS;

EID: 0029805129     PISSN: 02707306     EISSN: None     Source Type: Journal    
DOI: 10.1128/MCB.16.11.6046     Document Type: Article

References (49)

1. Adami, G. R., and G. G. Carmichael. 1987. The length but not the sequence of the polyoma virus late leader exon is important for both late RNA splicing and stability. Nucleic Acids Res. 15:2593-2610.
2. Adami, G. R., C. W. Marlor, N. L. Barrett, and G. G. Carmichael. 1989. Leader-to-leader splicing is required for the efficient production and accumulation of polyomavirus late mRNAs. J. Virol. 63:85-93.
3. Alonso-Caplen, F. V., M. E. Nemeroff, Y. Qiu, and R. M. Krug. 1992. Nucleocytoplasmic transport: the influenza virus NS1 protein regulates the transport of spliced NS2 mRNA and its precursor NS1 mRNA. Genes Dev. 6:255-267.
4. Ausubel, F. M., R. Brent, R. E. Kingston, D. D. Moore, J. A. Smith, J. G. Seidman, and K. Struhl (ed.). 1989. Current protocols in molecular biology. Greene Publishing Associates and Wiley-Interscience, New York.
5. Barrett, N. L., G. G. Carmichael, and Y. Luo. 1991. Splice site requirement for the efficient accumulation of polyoma virus late mRNAs. Nucleic Acids Res. 19:3011-3017.
6. Barrett, N. L., X. Li, and G. G. Carmichael. 1995. The sequence and context of the 5′ splice site govern the nuclear stability of polyoma virus late RNAs. Nucleic Acids Res. 23:4812-4817.
7. Batt, D. B., L. M. Rapp, and G. G. Carmichael. 1994. Splice site selection in polyomavirus late pre-mRNA processing. J. Virol. 68:1797-1804.
8. Bray, M., S. Prasad, J. W. Dubay, E. Hunter, K. T. Jeang, D. Rekosh, and M. L. Hammarskjold. 1994. A small element from the Mason-Pfizer monkey virus genome makes human immunodeficiency virus type 1 expression and replication Rev-independent. Proc. Natl. Acad. Sci. USA 91:1256-1260.
9. Buchman, A. R., and P. Berg. 1988. Comparison of intron-dependent and intron-independent gene expression. Mol. Cell. Biol. 8:4395-4405.
10. Cahill, K. B., and G. G. Carmichael. 1989. Deletion analysis of the polyomavirus late promoter: evidence for both positive and negative elements in the absence of early proteins. J. Virol. 63:3634-3642.
11. Chang, D. D., and P. A. Sharp. 1989. Regulation by HIV rev depends upon recognition of splice sites. Cell 59:789-795.
12. Cullen, B. R. 1992. Mechanism of action of regulatory proteins encoded by complex retroviruses. Microbiol. Rev. 56:375-394.
13. Fischer, U., J. Huber, W. C. Boelens, I. W. Mattaj, and R. Luhrmann. 1995. The HIV-1 rev activation domain is a nuclear export signal that accesses an export pathway used by specific cellular RNAs. Cell 82:475-483.
14. Fischer, U., S. Meyer, M. Teufer, C. Heckel, R. Lührmann, and G. Rautmann. 1994. Evidence that HIV-1 Rev directly promotes the nuclear export of unspliced RNA. EMBO J. 13:4105-4112.
15. Green, M. R. 1991. Biochemical mechanisms of constitutive and regulated pre-mRNA splicing. Annu. Rev. Cell Biol. 7:559-599.
16. Green, M. R. 1989. Pre-mRNA processing and mRNA nuclear export. Curr. Opin. Cell Biol. 1:519-525.
17. Gruss, P., C.-J. Lai, R. Dhar, and G. Khoury. 1979. Splicing as a requirement for biogenesis of functional 16S mRNA of simian virus 40. Proc. Natl. Acad. Sci. USA 76:4317-4321.
18. Hamer, D., K. Smith, S. Boyer, and P. Leder. 1979. SV40 recombinants carrying rabbit beta-globin coding sequences. Cell 17:725-735.
19. Huang, J., and T. J. Liang. 1993. A novel hepatitis B virus (HBV) genetic element with Rev response element-like properties that is essential for expression of HBV gene products. Mol. Cell. Biol. 13:7476-7486.
20. Huang, Y., and G. G. Carmichael. 1996. Role of polyadenylation in nucleocytoplasmic transport of mRNA. Mol. Cell. Biol. 16:1534-1542.
21. 20a. Huang, Y., and G. G. Carmichael. Unpublished results.
22. Huang, Z.-M., and T. S. B. Yen. 1995. Role of the hepatitis B virus posttranscriptional regulatory element in export of intronless transcripts. Mol. Cell. Biol. 15:3864-3869.
23. Hyde-DeRuyscher, R. P., and G. G. Carmichael. 1990. Polyomavirus late pre-mRNA processing: DNA-replication-associated changes in leader exon multiplicity suggest a role for leader-to-leader splicing in the early-late switch. J. Virol. 64:5823-5832.
24. Izaurralde, E., and I. W. Mattaj. 1995. RNA export. Cell 81:153-159.
25. Kalland, K. H., A. M. Szilvay, E. Langhoff, and G. Haukenes. 1994. Subcellular distribution of human immunodeficiency virus type 1 rev and colocalization of rev with RNA splicing factors in a speckled pattern in the nucleoplasm. J. Virol. 68:1475-1485.
26. Katz, R. A., and A. M. Skalka. 1990. Control of retroviral RNA splicing through maintenance of suboptimal processing signals. Mol. Cell. Biol. 10: 696-704.
27. Kjems, J., and P. A. Sharp. 1993. The basic domain of rev from human immunodeficiency virus type-1 specifically blocks the entry of U4/U6 u5 small nuclear ribonucleoprotein in spliceosome assembly. J. Virol. 67:4769-4776.
28. Krug, R. M. 1993. The regulation of export of mRNA from nucleus to cytoplasm. Curr. Opin. Cell Biol. 5:944-949.
29. Legrain, P., and M. Rosbash. 1989. Some cis- and trans-acting mutants for splicing target pre-mRNA to the cytoplasm. Cell 57:573-583.
30. Lichtler, A., N. L. Barrett, and G. G. Carmichael. 1992. Simple, inexpensive preparation of T1/T2 ribonuclease suitable for use in RNase protection experiments. BioTechniques 12:231-232.
31. Liu, X. D., and J. E. Mertz. 1995. HnRNP L binds a cis-acting RNA sequence element that enables intron-independent gene expression. Genes Dev. 9:1766-1780.
32. Lu, X., J. Heimer, D. Rekosh, and M.-L. Hammarskjöld. 1990. U1 small nuclear RNA plays a direct role in the formation of a rev-regulated human immunodeficiency virus env mRNA that remains unspliced. Proc. Natl. Acad. Sci. USA 87:7598-7602.
33. Luo, Y., and G. G. Carmichael. 1991. Splice site choice in a complex transcription unit containing multiple inefficient polyadenylation signals. Mol. Cell. Biol. 11:5291-5300.
34. Malim, M. H., and B. R. Cullen. 1993. Rev and the fate of pre-mRNA in the nucleus: implications for the regulation of RNA processing in eukaryotes. Mol. Cell. Biol. 13:6180-6189.
35. Malim, M. H., J. Hauber, S.-Y. Le, J. V. Maizel, and B. R. Cullen. 1989. The HIV-1 rev transactivator acts through a structured target sequence to activate nuclear export of unspliced viral mRNA. Nature (London) 338:254-257.
36. Maniatis, T., E. F. Fritsch, and J. Sambrook. 1982. Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
37. Mattaj, I. W. 1990. Splicing stories and poly(A) tales: an update on RNA processing and transport. Curr. Opin. Cell Biol. 2:528-538.
38. McNally, M. T., and K. Beemon. 1992. Intronic sequences and 3′ splice sites control Rous sarcoma virus RNA splicing. J. Virol. 66:6-11.
39. Meyer, B. E., and M. H. Malim. 1994. The HIV-1 Rev trans-activator shuttles between the nucleus and the cytoplasm. Genes Dev. 8:1538-1547.
40. Rosbash, M., and R. H. Singer. 1993. RNA travel - tracks from DNA to cytoplasm. Cell 75:399-401.
41. Ruhl, M., M. Himmelspach, G. M. Bahr, F. Hammerschmid, H. Jaksche, B. Wolff, H. Aschauer, G. K. Farrington, H. Probst, D. Bevec, and J. Hauber. 1993. Eukaryotic initiation factor 5A is a cellular target of the human immunodeficiency virus type 1 Rev activation domain mediating trans-activation. J. Cell Biol. 123:1309-1320.
42. Ryu, W. S., and J. E. Mertz. 1989. Simian virus 40 late transcripts lacking excisable intervening sequences are defective in both stability in the nucleus and transport to the cytoplasm. J. Virol. 63:4386-4394.
43. Seraphin, B., and M. Rosbash. 1989. Identification of functional U1 snRNA pre-messenger RNA complexes committed to spliceosome assembly and splicing. Cell 59:349-358.
44. Sharp, P. A. 1987. Speculations on messenger RNA precursors. Science 235:766-771.
45. Smith, C. W. J., J. G. Patton, and B. Nadal-Ginard. 1989. Alternative splicing in the control of gene expression. Annu. Rev. Genet. 23:527-577.
46. Stutz, F., M. Neville, and M. Rosbash. 1995. Identification of a novel nuclear pore-associated protein as a functional target of the HIV-1 rev protein in yeast. Cell 82:495-506.
47. Treisman, R. 1980. Characterization of polyoma late mRNA leader sequences by molecular cloning and DNA sequence analysis. Nucleic Acids Res. 8:4867-4888.
48. Zapp, M. L. 1995. The ins and outs of RNA nucleocytoplasmic transport. Curr. Opin. Genet. Dev. 5:229-233.
49. Zhuang, Y., and A. M. Weiner. 1986. A compensatory base change in U1 snRNA suppresses a 5′ splice site mutation. Cell 46:827-835.