Recent developments in flow cytometry

Journal of Clinical Immunology

Volumn 20, Issue 6, 2000, Pages 400-407

  Scheffold, Alexander ^a   Kern, Florian ^b  

^a DEUTSCHES RHEUMA FORSCHUNGSZENTRUM   (Germany)

^b CHARITÉ UNIVERSITÄTSMEDIZIN BERLIN   (Germany)

Author keywords

Affinity matrix; Cytokine; Intracellular immunofluorescence; Magnetofluorescent liposomes; Major histocompatibility complex tetramer

Indexed keywords

CARBOXYFLUORESCEIN DIACETATE SUCCINIMIDYL ESTER; CYTOKINE; HLA ANTIGEN; MAJOR HISTOCOMPATIBILITY ANTIGEN; T LYMPHOCYTE RECEPTOR; TETRAMER;

ANALYTIC METHOD; ANTIBODY LABELING; ANTIGEN SPECIFICITY; ARTICLE; B LYMPHOCYTE; CELL SELECTION; CYTOKINE PRODUCTION; DIAGNOSTIC VALUE; FLOW CYTOMETRY; FLUORESCENCE ACTIVATED CELL SORTER; HUMAN; HUMAN CELL; IMMUNOFLUORESCENCE; LYMPHOCYTE PROLIFERATION; MAGNETISM; MAJOR HISTOCOMPATIBILITY COMPLEX; PARAMETER; PRIORITY JOURNAL; T LYMPHOCYTE;

EID: 0034493405     PISSN: 02719142     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1026454225515     Document Type: Article

References (56)

1. Detection of receptors for cytokines and growth factors
2. Expression of surrogate light chain receptors is restricted to a late stage in pre-B cell differentiation
3. Threshold of pre-T-cell-receptor surface expression is associated with alphabeta T-cell lineage commitment
4. Magnetofluorescent liposomes for increased sensitivity of immunofluorescence
5. High-sensitivity immunofluorescence for detection of the pro- and anti-inflammatory cytokines gamma interferon and interleukin-10 on the surface of cytokine-secreting cells
6. Analysis and sorting of T cells according to cytokine expression
7. Specific expression of surface interferon-gamma on interferon-gamma producing T cells from mouse and man
8. Transfer of IFN-gamma-depleted CD4(+) T cells together with CD8(+) T cells leads to rejection of murine kidney sarcoma in mice
9. Signal amplification in flow cytometry using biotin tyramine
10. Enzymatic signal amplification for sensitive detection of intracellular antigens by flow cytometry
11. Enzymatic amplification staining for flow cytometric analysis of cell surface molecules
12. Analysis and sorting of live cells according to secreted molecules, relocated to a cell-surface affinity matrix
13. Lifetime of plasma cells in the bone marrow
14. Survival of long-lived plasma cells is independent of antigen
15. Sequential production of IL-2, IFN-gamma and IL-10 by individual staphylococcal enterotoxin B-activated T helper lymphocytes
16. Enrichment and detection of live antigen-specific CD4(+) and CD8(+) T cells based on cytokine secretion
17. Stat6-independent GATA-3 autoactivation directs IL-4-independent Th2 development and commitment
18. Measurement of cellular proliferation
19. Analysis of DNA content and cyclin protein expression in studies of DNA ploidy, growth fraction, lymphocyte stimulation, and the cell cycle
20. Simultaneous flow cytometric detection of bromode-oxyuridine incorporation and cell surface marker expression
21. Determination of lymphocyte division by flow cytometry
22. Divided we stand: Tracking cell proliferation with carboxyfluorescein diacetate succinimidyl ester
23. Measurement of Candida-specific blastogenesis: Comparison of carboxyfluorescein succinimidyl ester labeling of T cells, thymidine incorporation, and CD69 expression
24. B cell differentiation and isotype switching is related to division cycle number
25. Instruction for cytokine expression in T helper lymphocytes in relation to proliferation and cell cycle progression
26. Cell division regulates the T cell cytokine repertoire, revealing a mechanism underlying immune class regulation
27. Helper T cell differentiation is controlled by the cell cycle
28. Nine-color eleven-parameter immunophenotyping using three-laser flow cytometry
29. Counting antigen-specific CD8 T cells: A reevaluation of bystander activation during viral infection
30. Massive expansion of antigen-specific CD8+ T cells during an acute virus infection
31. MHC class II tetramers identify peptide-specific human CD4+ T cells proliferating in response to influenza A antigen
32. Diversity of human alpha beta T cell receptors
33. Size estimate of the alpha beta TCR repertoire of naive mouse splenocytes
34. Immunomagnetic enrichment of disseminated epithelial tumor cells from peripheral blood by MACS
35. Maintenance of B-cell memory by long-lived cells generated from proliferating precursors
36. Isolation and characterization of allergen-binding cells from normal and allergic donors
37. Survival of long-lived plasma cells is independent of antigen
38. Correlation analysis between frequencies of circulating antigen-specific IgG-bearing memory B cells and serum titers of antigen-specific IgG
39. Phenotypic analysis of antigen-specific T lymphocytes
40. Direct visualization of antigen-specific CD8+ T cells during the primary immune response to Epstein-Barr virus in vivo
41. Quantitative analysis of hepatitis C virus-specific CD8(+) T cells in peripheral blood and liver using peptide-MHC tetramers
42. Ex vivo staining of metastatic lymph nodes by class I major histocompatibility complex tetramers reveals high numbers of antigen-experienced tumor-specific cytolytic T lymphocytes
43. Isolation of high avidity melanoma-reactive CTL from heterogeneous populations using peptide-MHC tetramers
44. In situ tetramer staining of antigen-specific T cells in tissues
45. Detection of antigen-specific T cells with multivalent soluble class II MHC covalent peptide complexes
46. Use of soluble peptide-DR4 tetramers to detect synovial T cells specific for cartilage antigens in patients with rheumatoid arthritis
47. The relationship of MHC-peptide binding and T cell activation probed using chemically defined MHC class II oligomers
48. Detection of intracellular cytokines by flow cytometry
49. Flow cytometric determination of cytokines in activated murine T helper lymphocytes: Expression of interleukin-10 in interferon-gamma and in interleukin-4-expressing cells
50. Determination of antigen-specific memory/effector CD4+ T cell frequencies by flow cytometry: Evidence for a novel, antigen-specific homeostatic mechanism in HIV-associated immunodeficiency
51. T-cell epitope mapping by flow cytometry
52. Target structures of the CD8(+)-T-cell response to human cytomegalovirus: The 72-kilodalton major immediate-early protein revisited
53. Analysis of CD8 T cell reactivity to cytomegalovirus using protein-spanning pools of overlapping pentadecapeptides
54. HIV-specific CD8(+) T cells produce antiviral cytokines but are impaired in cytolytic function