Divided we stand: Tracking cell proliferation with carboxyfluorescein diacetate succinimidyl ester

Immunology and Cell Biology

Volumn 77, Issue 6, 1999, Pages 509-515

  Lyons, A Bruce ^a  

^a UNIVERSITY OF TASMANIA   (Australia)

Author keywords

Carboxyfluorescein diacetate succinimidyl ester; Dye dilution; Lymphocyte division

Indexed keywords

BROXURIDINE; CARBOXYFLUORESCEIN DIACETATE SUCCINIMIDYL ESTER; FLUORESCENT DYE; HOE 33342; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; CELL DIFFERENTIATION; CELL DIVISION; CELL LABELING; CONTROLLED STUDY; FLOW CYTOMETRY; IMMUNE SYSTEM; LYMPHOCYTE PROLIFERATION; MOUSE; NONHUMAN; STAINING; T LYMPHOCYTE;

ANIMALS; CELL DIVISION; CELL MEMBRANE; CELL SURVIVAL; FLOW CYTOMETRY; FLUORESCEINS; FLUORESCENT DYES; LYMPHOCYTE SUBSETS; LYMPHOCYTE TRANSFUSION; LYMPHOCYTES; MICE; MICE, INBRED BALB C; SUCCINIMIDES;

ANIMALIA;

EID: 0032712575     PISSN: 08189641     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1440-1711.1999.00864.x     Document Type: Article

References (18)

1. 1 Lyons AB, Parish CR. Determination of lymphocyte division by flow cytometry. J. Immunol. Meth. 1994; 171: 131-7.
2. 2 Weston SA, Parish CR. New fluorescent dyes for lymphocyte migration studies. Analysis by flow cytometry and fluorescence microscopy. J. Immunol. Meth. 1990; 133: 87-97.
3. 3 Lyons AB. Pertussis toxin pretreatment alters the in vivo division behaviour and survival of B lymphocytes, after intravenous transfer. Immunol. Cell Biol. 1997; 75: 7-12.
4. 4 Gett AV, Hodgkin PD. Cell division regulates the T cell cytokine repertoire, revealing a mechanism underlying immune class regulation. Proc. Natl Acad. Sci. USA 1998; 95: 9488-93.
5. 5 Bird JJ, Brown DR, Mullen AC et al. Helper T cell differentiation is controlled by the cell cycle. Immunity 1998; 9: 229-37.
6. 6 Kurts C, Carbone FR, Barnden M et al. CD4+ T cell help impairs CD8+ T cell deletion induced by cross-presentation of self-antigens and favors autoimmunity. J. Exp. Med. 1997; 186: 2057-62.
7. 7 Lee WT, Pelletier WJ. Visualizing memory phenotype development after in vitro stimulation of CD4+ T cells. Cell. Immunol. 1998; 188: 1-11.
8. 8 Hodgkin PD, Lee JH, Lyons AB. B cell differentiation and isotype switching is related to division cycle number. J. Exp. Med. 1996; 184: 277-81.
9. 9 Hasbold J, Lyons AB, Kehry MR, Hodgkin PD. Cell division number regulates IgG1 and IgE switching of B cells following CD40L and IL-4 stimulation. Eur. J. Immunol. 1998; 28: 1040-51.
10. 10 Kindler V, Zubler RH. Memory, but not naive, peripheral blood B lymphocytes differentiate into Ig-secreting cells after CD40 ligation and costimulation with IL-4 and the differentiation factors IL-2, IL-10, and IL-3. J. Immunol. 1997; 159: 2085-90.
11. 11 Hodgkin PD, Bartell G, Mamchak A, Doherty KV, Lyons AB, Hasbold J. The importance of efficacy and partial agonism in evaluating models of B lymphocyte activation. Int. Rev. Immunol. 1997; 15: 101-27.
12. 12 Wells AD, Gudmundsdottir H, Turka LA. Following the fate of individual T cells throughout activation and clonal expansion. Signals from T cell receptor and CD28 differentially regulate the induction and duration of a proliferative response. J. Clin. Invest. 1997; 100: 3173-318.
13. 13 Nordon RE, Ginsberg SS, Eaves CJ. High resolution cell division tracking demonstrates the Flt3 ligand dependence of human marrow CD34+CD38-cell production in vitro. Br. J. Haematol. 1997; 98: 528-39.
14. 14 Warren HS, Kinnear BF. Quantitative analysis of the effect of CD16 ligation on human NK cell proliferation. J. Immunol. 1999; 162: 735-42.
15. 15 Angulo R, Fulcher DA. Measurement of Candida-specific blastogenesis: Comparison of carboxyfluorescein succinimidyl ester labelling of T cells, thymidine incorporation, and CD69 expression. Cytometry 1998; 34: 143-51.
16. 16 Fulcher DA, Lyons AB, Korn S et al. The fate of self-reactive B-cells depends primarily on the degree of antigen receptor engagement and availability of T-cell help. J. Exp. Med. 1996; 183: 2313-28.
17. 17 Lyons AB, Doherty KV. Flow cytometric analysis of cell division by dye dilution. In: Robinson JP, Darzynkiewicz Z, Dean P et al. (eds). Current Protocols in Cytometry. New York: John Wiley and Sons, 1998; Unit 9.11.
18. 18 Lyons AB, Hasbold J, Hodgkin PD. Flow cytometric analysis of cell division history using dilution of CFSE, a stably integrated fluorescent probe. In: Darzynkiewicz Z, Crissman HA, Robinson JP (eds). Methods in Cell Biology; Cytometry. San Diego: Academic Press, 1999; in press.