MHC class II tetramers identify peptide-specific human CD4+ T cells proliferating in response to influenza A antigen

Journal of Clinical Investigation

Volumn 104, Issue 12, 1999, Pages

  Novak, Erik J ^a,b   Liu, Andrew W ^a   Nepom, Gerald T ^a   Kwok, William W ^a  

^a BENAROYA RESEARCH INSTITUTE   (United States)

^b UNIVERSITY OF WASHINGTON   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIGEN; CD3 ANTIGEN; CD4 ANTIGEN; CD8 ANTIGEN; EPITOPE; INFLUENZA VACCINE; INTERLEUKIN 2 RECEPTOR; MAJOR HISTOCOMPATIBILITY ANTIGEN CLASS 2; TETRAMER;

ANTIGEN DETECTION; ANTIGEN SPECIFICITY; ARTICLE; CELL DIVISION; CELL PROLIFERATION; CONTROLLED STUDY; HELPER CELL; HUMAN; HUMAN CELL; IMMUNE RESPONSE; INFLUENZA VIRUS A; MAJOR HISTOCOMPATIBILITY COMPLEX; PRIORITY JOURNAL; T LYMPHOCYTE; T LYMPHOCYTE ACTIVATION;

EID: 0033392612     PISSN: 00219738     EISSN: None     Source Type: Journal    
DOI: 10.1172/JCI8476     Document Type: Article

References (23)

1. Goverman, J., et al. 1993, Transgenic mice that express a myelin basic protein-specific T cell receptor develop spontaneous autoimmunity. Cell. 72:551-560.
2. Kaye, J., et al. 1989. Selective development of CD4+ T cells in transgenic mice expressing a class II MHC-restricted antigen receptor. Nature. 341:746-749.
3. Sagerstrom, C.G., Kerr, E.M., Allison, J.P., and Davis, M.M. 1993. Activation and differentiation requirements of primary T cells in vitro. Proc. Natl. Acad. Sci. USA. 90:8987-8991.
4. Scott, B., Bluthmann, H., Teh, H.S., and von Boehmer, H. 1989. The generation of mature T cells requires interaction of the alpha beta T-cell receptor with major histocompatibility antigens. Nature. 338:591-593.
5. Bot, A., Casares, S., Bot, S., von Boehmer, H., and Bona, C. 1998. Cellular mechanisms involved in protection against influenza virus infection in transgenic mice expressing a TCR receptor specific for class II hemagglutinin peptide in CD4+ and CDS+ T cells. J. Immunol. 160:4500-4507.
6. Ota, K., et al. 1990. T-cell recognition of an immunodominant myelin basic protein epitope in multiple sclerosis. Nature. 346:183-187.
7. Tuohy, V.K., Yu, M., Yin, L., Kawczak, J.A., and Kinkel, R.P. 1999. Spontaneous regression of primary autoreactivity during chronic progression of experimental autoimmune encephalomyelitis and multiple sclerosis. J. Exp. Med. 189:1033-1042.
8. Altman, J.D., et al. 1996. Phenotypic analysis of antigen-specific T lymphocytes. Science. 274:94-96.
9. Lee, P.P., et al. 1999. Characterization of circulating T cells specific for tumor-associated antigens in melanoma patients. Nat. Med. 5:677-685.
10. Romero, P., et al. 1998. Ex vivo staining of metastatic lymph nodes by class I major histocompatibility complex tetramers reveals high numbers of antigen-experienced tumor-specific cytolytic T lymphocytes. J. Exp. Med. 188:1641-1650.
11. Yee, C., Savage, P.A., Lee, P.P., Davis, M.M., and Greenberg, P.D. 1999. Isolation of high avidity melanoma-reactive CTL from heterogeneous populations using peptide-MHC tetramers. J. Immunol. 162:2227-2234.
12. Crawford, F., Kozono, H., White, J., Marrack, P., and Kappler, J. 1998. Detection of antigen-specific T cells with multivalent soluble class II MHC covalent peptide complexes. Immunity. 8:675-682.
13. Horton, R.M., Cai, Z.L., Ho, S.N., and Pease, L.R. 1990. Gene splicing by overlap extension: tailor-made genes using the polymerase chain reaction. Biotechniques. 8:528-535.
14. Chang, H.C., ct al, 1994. A general method for facilitating heterodimeric pairing between two proteins: application to expression of alpha and beta T-cell receptor extracellular segments. Proc. Natl. Acad. Sci. USA. 91:11408-11412.
15. Stern, L.J., and Wiley, D.C. 1992. The human class II MHC protein HLA-DR1 assembles as empty alpha beta heterodimers in the absence of antigenic peptide. Cell. 68:465-477.
16. Schatz, P.J. 1993. Use of peptide libraries to map the substrate specificity of a peptide-modifying enzyme: a 13 residue consensus peptide specifies biotinylation in Escherichia coll. Biotechnology. 11:1138-1143.
17. Mickelson, E.M., Masewicz, S.A., Nepom, G.T., Martin, P.J., and Hansen, J.A. 1991. Alloreactive T-cell clones identify multiple HLA-LQw3 variants. Hum. Immunol. 30:32-40.
18. Kovats, S., et al. 1995 Deficient antigen-presenting cell function in multiple genetic complementation groups of type II bare lymphocyte syndrome. J. Clin. Invest. 96:217-223.
19. Lamb, J.R., Eckels, D.D., Lake, P., Woody, J.N., and Green, N. 1982. Human T-cell clones recognize chemically synthesized peptides of influenza haemagglutinin. Nature. 300:66-69.
20. Krieger, J.I., et al. 1991. Single amino acid changes in DR and antigen define residues critical for peptide-MHC binding and T cell recognition. J. Immunol. 146:2331-2340.
21. Panina-Bordignon, P., et al. 1989. Universally immunogenic T cell epitopes: promiscuous binding to human MHC class II and promiscuous recognition by T cells. Eur. J. Immunol. 19:2237-2242.
22. Lyons, A.B., and Parish, C.R. 1994. Determination of lymphocyte division by flow cytometry. J. Immunol. Methods. 171:131-137.
23. Stern, L.J., et al. 1994. Crystal structure of the human class II MHC protein HLA-DR1 complexed with an influenza virus peptide. Nature. 368:215-221.