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Volumn 286, Issue 5441, 1999, Pages 958-961

A direct estimate of the human αβ T cell receptor diversity

Author keywords

[No Author keywords available]

Indexed keywords

T LYMPHOCYTE RECEPTOR;

EID: 0033615678     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.286.5441.958     Document Type: Article
Times cited : (745)

References (40)
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    • The CDR3 sequences were extracted from the raw sequence data with software developed by E. Beaudoing and others at Information Genetique et Structurale, CNRS, Marseille, France. Because of the infidelity of the Taq DNA polymerase, we excluded from the analysis sequences that were likely to represent polymerase chain reaction (PCR) mistakes, defined as sequences differing from another sequence by a single nucleotide. Analysis of conserved areas in the V gene showed that the actual rate of PCR mistakes was 1.6 per 1000 base pairs, that is, a mistake in 5% of a set of 10-amino acid segments. Our criterium resulted in the exclusion of 6% of the CDR3 sequences, a rate slightly in excess of the predicted amount. Thus, the data reported may be a slight underestimate.
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    • We thank P. Bousso, A. Cumano, A. Vaheri, O. Vapalahti, and M. Viljanen for comments on the manuscript. Supported by the European Molecular Biology Organization and Academy of Finland (T.P.A.) and Ligue Nationale Contre le Cancer.


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