The N-terminal 34 kDa fragment of Helicobacter pylori vacuolating cytotoxin targets mitochondria and induces cytochrome c release

EMBO Journal

Volumn 19, Issue 23, 2000, Pages 6361-6370

  Galmiche, Antoine ^a   Rassow, Joachim ^b   Doye, Anne ^a   Cagnol, Sebastien ^c   Chambard, Jean Claude ^c   Contamin, Stephanette ^a   De Thillot, Virginie ^a   Just, Ingo ^d   Ricci, Vittorio ^a   Solcia, Enrico ^e   Van Obberghen, Ellen ^c   Boquet, Patrice ^a  

^a INSERM   (France)

^b UNIVERSITY OF HOHENHEIM   (Germany)

^c CENTRE ANTOINE LACASSAGNE   (France)

^d HANNOVER MEDICAL SCHOOL   (Germany)

^e UNIVERSITY OF PAVIA   (Italy)

Author keywords

Apoptosis; Bcl 2; Green fluorescent protein; Helicobacter pylori vacuolating cytotoxin

Indexed keywords

BACTERIAL TOXIN; CYTOTOXIN;

AMINO TERMINAL SEQUENCE; APOPTOSIS; ARTICLE; BACTERIAL VIRULENCE; CELL STRAIN HEPG2; CONTROLLED STUDY; ENZYME RELEASE; HELA CELL; HELICOBACTER PYLORI; HUMAN; HUMAN CELL; IMMUNOCYTOCHEMISTRY; IMMUNOFLUORESCENCE MICROSCOPY; MITOCHONDRION; NONHUMAN; PRIORITY JOURNAL; TRANSMISSION ELECTRON MICROSCOPY;

BACTERIA (MICROORGANISMS); HELICOBACTER PYLORI;

EID: 0034388020     PISSN: 02614189     EISSN: None     Source Type: Journal    
DOI: 10.1093/emboj/19.23.6361     Document Type: Article

References (60)

1. The clinical relevance of strain types of Helicobacter pylori
2. Detection of apoptosis by annexin V labelling
3. Biochemical pathways of caspase activation during apoptosis
4. High efficiency transformation of mammalian cells by plasmid DNA
5. The vacuolating cytotoxin of Helicobacter pylori
6. Acid-induced dissociation of VacA, the Helicobacter pylori vacuolating cytotoxin, reveals its pattern of assembly
7. Potentiation of Helicobacter pylori vacuolating toxin activity by nicotine and other weak bases
8. The vacuolating toxin from Helicobacter pylori forms hexameric pores in lipid bilayers at low pH
9. 2 and cytochrome c peroxidase am located in the intermembrane space of yeast mitochondria
10. Low pH activates the vacuolating toxin of Helicobacter pylori which becomes acid and pepsin resistant
11. Helicobacter pylori toxin VacA induces vacuole formation by acting in the cell cytosol
12. Identification of the Helicobacter pylori VacA toxin domain active in the cell cytosol
13. The VacA toxin of Helicobacter pylori identifies a new intermediate filament-interacting protein
14. Penetration of protein toxins into cells Curr
15. Requirement for prolonged action in the cytosol for optimal protein synthesis inhibition by diphtheria toxin
16. Prevention of vertebrate neuronal death by the CrmA gene
17. The modulation of host cell apoptosis by intracellular bacterial pathogens
18. Inhibition of human caspases by peptide-based and macromolecular inhibitors
19. Binding and internalization of the Helicobacler pylori vacuolating cytotoxin by epithelial cells
20. Tight control of gene expression in mammalian cells by tetracycline-responsive promoters
21. VacA from Helicobacter pylori: A hexameric chloride channel
22. Vacuolating cytotoxin purified from Helicobacter pylori causes mitochondrial damage in human gastric cells
23. Mitochondrial control of cell death
24. Biogenesis of Tim proteins of the mitochondrial carrier import pathway: Differential targeting mechanisms and crossing over with the main import pathway
25. Cleavage of poly(ADP-ribose) polymerase by a proteinase with properties like ICE
26. Natural diversity in the N-terminus of the mature vacuolating cytotoxin of Helicobacter pylori determines cytotoxin activity
27. Oligomeric and subunit structure of the Helicobacter pylori vacuolating cytotoxin
28. Binding of the Helicobacter pylori vacuolating cytotoxin to target cells
29. Acid activation of Helicobacter pylori vacuolating cytotoxin (VacA) results in toxin internalization by eukaryotic cells
30. Vacuoles induced by Helicobacterpylori toxin contain both late endosomal and lysosomal markers
31. The acid activation of Helicobacter pylori toxin VacA: Structural and membrane binding studies
32. Apoptosis by death factor
33. Protein import into mitochondria
34. The m2 form of the Helicobacter pylori cytotoxin has cell type-specific vacuolating activity
35. Cellular vacuoles induced by Helicobacter pylori originate from late endosomal compartments
36. Selective increase of the permeability of polarized epithelial cell monolayers by Helicobacter priori vacuolating toxin
37. Helicobacter pylori strain-specific genotypes and modulation of the gastric epithelial cell cycle
38. Helicobacter pylori VacA cytotoxin associated with the bacteria increases epithelial permeability independently of its vacuolating activity
39. Biogenesis of Tom40, core component of the TOM complex of mitochondria
40. Protein folding and import into organelles
41. Mitochondrial preproteins en route from the outer membrane to the inner membrane are exposed to the intermembrane space
42. Towards deciphering the Helicobacter pylori cytotoxin
43. Helicobacter pylori vacuolating toxin accumulates within the endosomal-vacuolar compartment of cultured gastric cells and potentiates the vacuolating activity of ammonia
44. Mitochondrial presequences
45. Involvement of the CD95 (APO-1/Fas) receptor and ligand system in Helicobacter pylori-induced gastric epithelial apoptosis
46. Blue native electrophoresis for isolation of membrane protein complexes in enzymatically active form
47. Direct membrane insertion of voltage-dependent anion-selective channel protein catalyzed by mitochondrial Tom20
48. Helicobacter pylori induced apoptosis
49. Persistence of Helicobacter pylori VacA toxin and vacuolating potential in cultured gastric epithelial cells
50. Formation of anion-selective channels in the cell plasma membrane by the toxin VacA of Helicobacter pylori is required for its biological activity
51. Gene structure of the Helicobacter pylori cytotoxin and evidence of its key role in gastric disease
52. Caspases: Enemies within
53. Helicobacter pylori vacuolating toxin forms anion-selective channels in planar lipid bilayers: Possible implications for the mechanism of cellular vacuolation
54. Bc12 proteins: Regulators of apoptosis or of mitochondrial homeostasis?
55. A dominant negative mutant of Helicobacter pylori vacuolating toxin (VacA) inhibits VacA-induced cell vacuolation
56. The induction of apoptosis by bacterial pathogens
57. Protein targeting to mitochondria
58. One molecule of diphtheria toxin fragment A introduced into a cell can kill the cell
59. Mutational analysis of the Helicobacter priori vacuolating cytotoxin amino terminus: Identification of amino acids essential for cellular vacuolation
60. Identification of the minimal intracellular vacuolating domain of the Helicobacter pylori vacuolating toxin