Persistence of Helicobacter pylori VacA toxin and vacuolating potential in cultured gastric epithelial cells

American Journal of Physiology - Gastrointestinal and Liver Physiology

Volumn 275, Issue 4 38-4, 1998, Pages

  Sommi, Patrizia ^a   Ricci, Vittorio ^a   Fiocca, Roberto ^a   Necchi, Vittorio ^a   Romano, Marco ^b   Telford, John L ^c   Solcia, Enrico ^a,d   Ventura, Ulderico ^a  

^a UNIVERSITY OF PAVIA   (Italy)

^b UNIVERSITY OF NAPLES FEDERICO II   (Italy)

^c Instituto Ricerche Immunobiol Siena   (Italy)

^d Dept of Human Pathology ^*  (Italy)

Author keywords

Outer membrane vesicles; VacA immunocytochemistry; VacA internalization; VacA metabolism

Indexed keywords

AMMONIUM CHLORIDE; BACTERIAL TOXIN; DYE; VIRULENCE FACTOR;

ARTICLE; BACTERIAL OUTER MEMBRANE; CONTROLLED STUDY; ELECTRON MICROSCOPY; EPITHELIUM CELL; HELICOBACTER PYLORI; HUMAN; HUMAN CELL; IMMUNOBLOTTING; IMMUNOCYTOCHEMISTRY; IMMUNOFLUORESCENCE; INTERNALIZATION; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; STOMACH EPITHELIUM; ULTRASTRUCTURE;

EID: 0031751816     PISSN: 01931857     EISSN: None     Source Type: Journal    
DOI: 10.1152/ajpgi.1998.275.4.g681     Document Type: Article

References (8)

1. Chavrier, P., R. G. Parton, H. P. Hauri, K. Simons, and M. Zerial. Localization of low molecular weight GTP binding proteins to exocytic and endocytic compartments. Cell 62: 317-329, 1990.
2. Cover, T. L. The vacuolating cytotoxin of Helicobacter pylori. Mol. Microbiol. 20: 241-246, 1996.
3. Cover, T. L., and M. J. Blaser. Purification and characterization of the vacuolating toxin from Helicobacter pylori. J. Biol. Chem. 267: 10570-10575, 1992.
4. Cover, T. L., C. P. Dooley, and M. J. Blaser. Characterization of and human serologic response to proteins in Helicobacter pylori broth culture supernatants with vacuolizing cytotoxic activity. Infect. Immun. 58: 603-610, 1990.
5. Cover, T. L., W. Puryear, G. I. Perez-Perez, and M. J. Blaser. Effect of urease on HeLa cell vacuolation induced by Helicobacter pylori cytotoxin. Infect. Immun. 59: 1264-1270, 1991.
6. Cover, T. L., M. K. R. Tummuru, P. Cao, S. A. Thompson, and M. J. Blaser. Divergence of genetic sequences for the vacuolating cytotoxin among Helicobacter pylori strains. J. Biol. Chem. 269: 10566-10573, 1994.
7. Cover, T. L., S. G. Vaughn, P. Cao, and M. J. Blaser. Potentiation of Helicobacter pylori vacuolating toxin activity by nicotine and other weak bases. J. Infect. Dis. 166: 1073-1078, 1992.
8. De Bernard, M., E. Papini, V. de Filippis, E. Gottard, J. Telford, R. Manetti, A. Fontana, R. Rappuoli, and C. Montecucco. Low pH activates the vacuolating toxin of Helicobacter pylori, which becomes acid and pepsin resistant. J. Biol. Chem. 270: 23937-23940, 1995.