Mutational analysis of the Helicobacter pylori vacuolating toxin amino terminus: Identification of amino acids essential for cellular vacuolation

Infection and Immunity

Volumn 68, Issue 7, 2000, Pages 4354-4357

  Ye, Dan ^a   Blanke, Steven R ^a  

^a University of Houston   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ALANINE; CYTOTOXIN;

AMINO TERMINAL SEQUENCE; ARTICLE; CELL VACUOLE; CONTROLLED STUDY; GENE DELETION; GENE MUTATION; HELA CELL; HELICOBACTER PYLORI; HUMAN; HUMAN CELL; PLASMID; POINT MUTATION; PRIORITY JOURNAL; SITE DIRECTED MUTAGENESIS; TOXIN ANALYSIS;

BACTERIAL PROTEINS; BACTERIAL TOXINS; GENETIC COMPLEMENTATION TEST; HELA CELLS; HELICOBACTER PYLORI; HUMANS; MUTATION; POINT MUTATION; SEQUENCE DELETION; VACUOLES; VIRULENCE;

EID: 0033942982     PISSN: 00199567     EISSN: None     Source Type: Journal    
DOI: 10.1128/IAI.68.7.4354-4357.2000     Document Type: Article

References (26)

1. Atherton, J. C. 1998. H. pylori virulence factors. Br. Med. Bull. 54:105-120.
2. Atherton, J. C., R. M. Peek, Jr., K. T. Tham, T. L. Cover, and M. J. Blaser. 1997. Clinical and pathological importance of heterogeneity in vacA, the vacuolating cytotoxin gene of Helicobacter pylori. Gastroenterology 112:92-99.
3. Cover, T. L. 1998. An intracellular target for Helicobacter pylori vacuolating toxin. Trends Microhiol. 6:127-128.
4. Cover, T. L. 1996. The vacuolating cytotoxin of Helicobacter pylori. Mol. Microbiol. 20:241-246.
5. Cover, T. L., and M. J. Blaser. 1996. Helicobacter pylori infection, a paradigm for chronic mucosal inflammation: pathogenesis and implications for eradication and prevention. Adv. Intern. Med. 41:85-117.
6. Cover, T. L., W. Puryear, G. I. Perez-Perez, and M. J. Blaser. 1991. Effect of urease on HeLa cell vacuolation induced by Helicobacter pylori cytotoxin. Infect. Immun. 59:1264-1270.
7. Cropley, I., G. Douce, M. Roberts, S. Chatfield, M. Pizza, I. Marsili, R. Rappuoli, and G. Dougan. 1995. Mucosal and systemic immunogenicity of a recombinant, non-ADP-ribosylating pertussis toxin: effects of formaldehyde treatment. Vaccine 13:1643-1648.
8. Cunningham, B. C., and J. A. Wells. 1989. High-resolution epitope mapping of hGH-receptor interactions by alanine-scanning mutagenesis. Science 244: 1081-1085.
9. de Bernard, M., B. Arico, E. Papini, R. Rizzuto, G. Grandi, R. Rappuoli, and C. Montecucco. 1997. Helicobacter pylori toxin VacA induces vacuole formation by acting in the cell cytosol. Mol. Microbiol. 26:665-674.
10. de Bernard, M., D. Burroni, E. Papini, R. Rappuoli, J. Telford, and C. Montecucco. 1998. Identification of the Helicobacter pylori VacA toxin domain active in the cell cytosol. Infect. Immun. 66:6014-6016.
11. Douce, G., C. Turcotte, I. Cropley, M. Roberts, M. Pizza, M. Domenghini, R. Rappuoli, and G. Dougan. 1995. Mutants of Escherichia coli heat-labile toxin lacking ADP-ribosyltransferase activity act as nontoxic, mucosal adjuvants. Proc. Natl. Acad. Sci. USA 92:1644-1648.
12. Fuerst, T. R., E. G. Niles, F. W. Studier, and B. Moss. 1986. Eukaryotic transient-expression system based on recombinant vaccinia virus that synthesizes bacteriophage T7 RNA polymerase. Proc. Natl. Acad. Sci. USA 83:8122-8126.
13. Garner, J. A., and T. L. Cover. 1996. Binding and internalization of the Helicobacter pylori vacuolating cytotoxin by epithelial cells. Infect. Immun. 64:4197-4203.
14. Ghiara, P., M. Marchetti, M. J. Blaser, M. K. R. Tummuru, T. L. Cover, E. D. Segal, L. S. Tompkins, and R. Rappuoli. 1995. Role of the Helicobacter pylori virulence factors vacuolating cytotoxin, CagA, and urease in a mouse model of disease. Infect. Immun. 63:4154-4160.
15. Leunk, R., P. Johnson, B. David, W. Kraft, and D. Morgan. 1988. Cytotoxin activity in broth culture filtrates of Campylobacter pylori. J. Med. Microbiol. 26:93-99.
16. Manetti, R., P. Massari, M. Marchetti, C. Magagnoli, S. Nuti, P. Lupetti, P. Ghiara, R. Rappuoli, and J. L. Telford. 1997. Detoxification of the Helicobacter pylori cytotoxin. Infect. Immun. 65:4615-4619.
17. Marchetti, M., B. Arico, D. Burroni, N. Figura, R. Rappuoli, and P. Ghiara. 1995. Development of a mouse model of Helicobacter pylori infection that mimics human disease. Science 267:1655-1658.
18. Phadnis, S. H., D. Ilver, L. Janzon, S. Normark, and T. U. Westblom. 1994. Pathological significance and molecular characterization of the vacuolating toxin gene of Helicobacter pylori. Infect. Immun. 62:1557-1565.
19. Roberts, M., A. Bacon, R. Rappuoli, M. Pizza, I. Cropley, G. Douce, G. Dougan, M. Marinaro, J. McGhee, and S. Chatfield. 1995. A mutant pertussis toxin molecule that lacks ADP-ribosyltransferase activity, PT-9K/ 129G, is an effective mucosal adjuvant for intranasally delivered proteins. Infect. Immun. 63:2100-2108.
20. Schimitt, W., and R. Haas, 1994. Genetic analysis of the Helicobacter pylori vacuolating cylotoxin: structural similarities with the IgA protease type of exported protein. Mol. Microbiol. 12:307-319.
21. Telford, J. L., P. Ghiara, M. Dell'Orco, M. Comanducci, D. Burroni, M. Bugnoli, M. F. Tecce, S. Censini, A. Covacci, Z. Xiang, et al. 1994. Gene structure of the Helicobacter pylori cytotoxin and evidence of its key role in gastric disease. J. Exp. Med 179:1653-1658.
22. van Doorn, L.-J., C. Figueiredo, R. Sanna, S. Pena, P. Midulo, E. K. W. Ng, J. C. Atherton, M. J. Blaser, and W. G. V. Quint. 1998. Expanding allelic diversity of Helicobacter pylori vacA. J. Clin. Microbiol. 36:2597-2603.
23. Vinion-Dubiel, A. D., M. S. McClain, D. M. Czajkowsky, H. Iwamoto, D. Ye, P. Cao, W. Schraw, G. Szabo, S. R. Blanke, Z. Shao, and T. L. Cover. 1999. A dominant negative mutant of Helicobacter pylori vacuolating toxin (VacA) inhibits VacA-induccd cell vacuolation. J. Biol. Chem. 274:37736-37742.
24. Wang, H. J., C. H. Kuo, A. A. Yeh, P. C. Chang, and W. C. Wang. 1998. Vacuolating toxin production in clinical isolates of Helicobacter pylori with different vacA genotypes. J. Infect. Dis. 178:207-212.
25. Xiang, Z., S. Censini, P. F. Bayeli, J. L. Telford, N. Figura, R. Rappuoli, and A. Covacci. 1995. Analysis of expression of CagA and VacA virulence factors in 43 strains of Helicobacter pylori reveals that clinical isolates can be divided into two major types and that CagA is not necessary for expression of the vacuolating cytotoxin. Infect. Immun. 63:94-98.
26. Ye, D., D. C. Willhite, and S. R. Blanke. 1999. Identification of the minimal intracellular vacuoluting domain of the Helicobacter pylori vacuolating toxin. J. Biol. Chem. 274:9277-9282.