Vacuolating cytotoxin purified from Helicobacter pylori causes mitochondrial damage in human gastric cells

Microbial Pathogenesis

Volumn 26, Issue 1, 1999, Pages 45-52

  Kimura, Miyuki ^a   Goto, Shinji ^a   Wada, Akihiro ^a   Yahiro, Kinnosuke ^a   Niidome, Takuro ^a   Hatakeyama, Tomomitsu ^a   Aoyagi, Haruhiko ^a   Hirayama, Toshiya ^a   Kondo, Takahito ^a  

^a NAGASAKI UNIVERSITY   (Japan)

Author keywords

Bacterial toxin; H. pylori; Mitochondrial damage; Toxin action; VacA; Vacuolating toxin

Indexed keywords

3,3' DIHEXYLOXACARBOCYANINE; ADENOSINE TRIPHOSPHATE; BAFILOMYCIN; CYTOTOXIN;

ARTICLE; CELL CYCLE G0 PHASE; CELL CYCLE G1 PHASE; CELL DAMAGE; CELL MEMBRANE POTENTIAL; CELL METABOLISM; CONTROLLED STUDY; CULTURE MEDIUM; EPITHELIUM CELL; FLOW CYTOMETRY; HELICOBACTER PYLORI; HUMAN; HUMAN CELL; MITOCHONDRIAL MEMBRANE; MITOCHONDRION; OXYGEN CONSUMPTION; PRIORITY JOURNAL; STOMACH EPITHELIUM;

BACTERIA (MICROORGANISMS); HELICOBACTER PYLORI; NEGIBACTERIA;

EID: 0006534562     PISSN: 08824010     EISSN: None     Source Type: Journal    
DOI: 10.1006/mpat.1998.0241     Document Type: Article

References (27)

1. Cover TL, Blaser MJ. Helicobacter pylori infection, a paradigm for chronic mucosal inflammation. Adv Intern Med 1996; 41: 85-117.
2. Leunk RD, Johnson PT, David BC, Kraft WG, Morgan DR. Cytotoxin activity in broth culture filtrates of Campylobacter pylori. J Med Microbiol 1988; 26: 93-9.
3. Cover TL, Blaser MJ. Purification and characterization of vacuolating toxin from Helicobacter pylori. J Biol Chem 1992; 267: 10570-5.
4. Lupetti P, Heuser JE, Manetti R, et al. Oligomeric and subunit structure of the Helicobacter pylori vacuolating cytotoxin. J Cell Biol 1996; 133: 801-7.
5. Cover TL, Tummuru MKR, Cao P, Thomas SA, Blaser MJ. Divergence of genetic sequence for the vacuolating cytotoxin among Helicobacter pylori strain. J Biol Chem 1994; 269: 10566-73.
6. Phdnis SH, Ilver D, Janzon L, Normark S, Westblom TU. Pathological significance and molecular characterization of the vacuolating toxin gene of Helicobacter pylori. Infect Immun 1994; 62: 1557-65.
7. Telford JL, Ghiara P, Dell'orco M, et al. Gene structure of the Helicobacter pylori cytotoxin and evidence of its key role in gastric disease. J Exp Med 1994; 179: 1653-8.
8. Shimitt W, Haas R. Genetic analysis of the Helicobacter pylori vacuolating cytotoxin : Structural similarities with the IgA protease type of exported protein. Mol Microbiol 1994; 12: 307-19.
9. Cover TL, Reddy YL, Blaser MJ. Effects of ATPase inhibitors on the response of Hela cell to Helicobacter pylori vacuolating toxin. Infect Immun 1993; 61: 1427-31.
10. Papini E, Bugnoli M, de Bernard M, Figura N, Rappouli R, Montecucco C. Bafilomycin A1 inhibits Helicobacter pylori vacuolization of Hela cells. Mol Microbiol 1993; 7: 323-7.
11. Papini E, de Bernard M, Bugnoli M, Milia E, Rappouli R, Montecucco C. Cell vacuolation induced by Helicobacter pylori: Inhibition by bafilomycin A1, B1, C1, and D. FEMS Microbiol 1993; 113: 155-9.
12. Papini E, Gotardi E, Stain B, et al. The vacuole ATPase proton pump on intracellular vacuoles induced by Helicobacter pylori. J Med Microbiol 1996; 44: 1-6.
13. Papini E, Satin B, Bucci C, et al. The small GTP binding protein rab7 is essential for cellular vacuolation induced by Helicobacter pylori. EMBO J 1997; 16: 15-24.
14. Chan EC, Chen K-T, Lin Y-L. Vacuolating toxin from Helicobacter pylori activates cellular signaling and pepsinogen secretion in human gastric adenocarcinoma cell. FEBS Lett. 1996; 399: 127-30.
15. Yahiro K, Niidome T, Hatakeyama T, et al. Helicobacter pylori vacuolating cytotoxin binds to the 140-kDa protein human gastric cancer cell lines, AZ-521 and AGS. Biochem Biophys Res Commun 1997; 238: 629-32.
16. Johnson LV, Summerhayes IC, Chen LB. Decreased uptake and retention of Rhodamine 123 by mitochondria in feline sarcoma virus-transformed mink cells. Cell 1982; 28: 7-14.
17. Bernard MD, Papini E, Filippis VD, et al. low pH activates the vacuolating toxin of Helicobacter pylori, which becomes acid and pepsin resistant. J Biol Chem 1995; 270: 23937-40.
18. Sakurai N, Koike KA, Irie Y, Hayashi H. The rice culture filtrate of Bacillus cereus isolated from Emic-type food poisoning causes mitochondrial swelling in a HEp-2 cell. Microbiol Immunol 1994; 38: 337-43.
19. Wei YH, Ding WH, Wei RD. Biochemical effects of PR toxin on rat mitochondrial respiration and oxidative phosphorylation. Arch Biochem Biophys 1984; 230: 400-11.
20. Wei YH, Lu TN, Wei RD. Effect of ochratoxin A on rat liver mitochondrial respiration and oxidative phosphorylation. Toxicology 1985; 36: 119-30.
21. Kato M. Effect of diphtherial cord factor and diphtherial toxin on mitochondrial oxidative phosphorylation. Jpn J Med Sci Biol 1972; 25: 230-5.
22. Turreus JF, ALexandra A, Lehninger AL. Ubisemiquinone is the electron donor for superoxide formation by complex III of heart mitochondria. Arch Biochem Biophys 1985; 237: 408-14.
23. Mannick EE, Bravo LE, Zarama G, Realpe JL, Zhang X-J, Ruiz B, Fontham ETH, Mera R, Miller MJS, Correa R. Inducible nitric oxide synthase, nitrotyrosine, and apotosis in Helicobacter pylori gastritis. Effect of antibiotics and antioxidants. Cancer Res. 1996; 56: 3238-43.
24. Beutler E. Red cell metabolism. In: Beutler E, Ed. A Manual of Biochemical Methods 3rd Edn. Orlando: Grune & Stratton, 1984: 122-4.
25. Vayssiere J-L, Petit P, Risler Y, Mignotte B. Commitment to apoptosis is associated with changes in mitochondrial biogenesis and activity in cell lines conditionally immortalized with simian virus 40. Proc Natl Acad Sci USA 1994; 91: 11752-6.
26. Gorczca W, Gong J, Ardelt B, Traganos F, Darzynkiewicz Z. The cell related differences in susceptibility of HL-60 cell to apoptosis induced by various antitumor agent. Cancer Res 1993; 53: 3186-92.
27. Kondo T, Tagami S, Yoshida A, Nishimura M, Kawakami Y. Current smoking of elderly men reduces antioxidants in alveolar macrophages. Am J Respir Crit Care Med 1994; 149: 178-82.