The VacA toxin of Helicobacter pylori identifies a new intermediate filament-interacting protein

EMBO Journal

Volumn 19, Issue 1, 2000, Pages 48-56

  De Bernard, Marina ^a   Moschioni, Monica ^a   Napolitani, Giorgio ^a   Rappuoli, Rino ^a,b   Montecucco, Cesare ^a  

^a UNIVERSITY OF PADOVA   (Italy)

^b CHIRON VACCINES   (Italy)

Author keywords

Cytotoxin; Helicobacter; Intermediate filaments; Vacuoles; Vimentin

Indexed keywords

ANTIBODY; BACTERIAL PROTEIN; BACTERIAL TOXIN; INTERMEDIATE FILAMENT PROTEIN;

ANIMAL CELL; ARTICLE; BRAIN; CELL CULTURE; CELL VACUOLE; ENDOSOME; HELA CELL; HELICOBACTER PYLORI; HUMAN; HUMAN CELL; HUMAN TISSUE; IMMUNOFLUORESCENCE MICROSCOPY; IMMUNOPRECIPITATION; INTERMEDIATE FILAMENT; KIDNEY; LIVER; LYSOSOME; MUSCLE; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN INTERACTION;

EID: 0034602831     PISSN: 02614189     EISSN: None     Source Type: Journal    
DOI: 10.1093/emboj/19.1.48     Document Type: Article

References (40)

1. Arcangeletti, C., Sütterlin, R., Aebi, U., De Conto, F., Missorini, S., Chezzi, C. and Scherrer, K. (1997) Visualization of prosomes (MCP-proteasomes), intermediate filament and actin networks by 'instantaneous fixation' preserving the cytoskeleton. J. Struct. Biol., 119, 35-58.
2. Chomczynski, P. and Sacchi, N. (1987) Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal. Biochem., 162, 156-159.
3. de Bernard, M., Aricò, B., Papini, E., Rizzuto, R., Grandi, G., Rappuoli, R. and Montecucco, C. (1997) Helicobacter pylori toxin VacA induces vacuole formation by acting in the cell cytosol. Mol. Microbiol., 26, 665-674.
4. de Bernard, M., Burroni, D., Papini, E., Rappuoli, R., Telford, J.L. and Montecucco, C. (1998) Identification of the Helicobacter pylori VacA toxin domain active in the cell cytosol. Infect. Immun., 66, 5414-5416.
5. Eger, A., Stockinger, A., Wiche, G. and Foisner, R. (1997) Polarisation-dependent association of plectin with desmoplakin and the lateral submembrane skeleton in MDCK cells. J. Cell Sci., 110, 1307-1310.
6. Fuchs, E. and Weber, K. (1994) Intermediate filaments: structure, dynamics, function, and disease. Annu. Rev. Biochem., 63, 345-382.
7. Gan, S.Q., McBride, O.W., Idler, W.W., Markowa, N. and Steinert, P.M. (1990) Organization, structure and polymorphism of the human profilaggrin gene. Biochemistry, 29, 9432-9470.
8. Garner, J.A. and Cover, T.L. (1996) Binding and internalization of the Helicobacter pylori vacuolating cytotoxin in epithelial cells. Infect. Immun., 46, 4197-4203.
9. Georgatos, S.D. and Blobel, G. (1987) Lamin B constitutes an intermediate filament attachment site at the nuclear envelope. J. Cell Biol., 105, 117-125.
10. Georgatos, S.D., Weaver, D.C. and Marchesi, V.T. (1985) Site specificity in vimentin-membrane interactions: intermediate filament subunits associate with the plasma membrane via their head domains. J. Cell Biol., 100, 1962-1967.
11. Granger, B.L. and Lazarides, E. (1980) Synemin: a new high molecular weight protein associated with desmin and vimentin filaments in muscle. Cell, 22, 727-738.
12. Iwamoto, H., Czajkowsky, D.M., Cover, T.L., Szabo, G. and Shao, Z. (1999) VacA from Helicobacter pylori: a hexameric chloride channel. FEBS Lett., 450, 101-104.
13. Knowles, B.B., Howe, C.C. and Aden, D.P. (1980) Human hepatocellular carcinoma cell lines secrete the major plasma proteins and hepatitis B surface antigen. Science, 209, 497-499.
14. Lawson, D. (1983) Epinemin: a new protein associated with vimentin filaments in non-neural cells. J. Cell Biol., 97, 1891-1905.
15. Lazarides, E. (1982) Intermediate filaments: a chemically heterogeneous developmentally regulated class of proteins. Annu. Rev. Biochem., 51, 219-250.
16. Levi, G., Aloisi, M., Ciotti, M. and Gallo, V. (1984) Autoradiographic localization and depolarization-induced release of amino acids in differentiating granule cell cultures. Brain Res., 290, 77-86.
17. Lupetti, P., Heuser, J.E., Manetti, R., Lanzavecchia, S., Bellon, P.L., Dallai, R., Rappuoli, R. and Telford, J.L. (1996) Oligomeric and subunit structure of the Helicobacter pylori vacuolating cytotoxin. J. Cell Biol., 133, 801-807.
18. Manetti, R. et al. (1995) Helicobacter pylori cytotoxin: importance of native conformation for induction of neutralizing antibodies. Infect. Immun., 63, 4476-4480.
19. Marshall, B.J., Armstrong, J.A., McGeche, D.B. and Glancy, R.J. (1985) Attempt to fulfil Koch's postulates for pyloric Campylobacter. Med. J. Aust., 142, 436-439.
20. Molinari, M., Galli, C., Norais, N., Telford, J.L., Rappuoli, R., Luzio, J.P. and. Montecucco, C. (1997) Vacuoles induced by Helicobacter pylori toxin contain both late endosomal and lysosomal markers. J. Biol. Chem., 272, 25339-25344.
21. Molinari, M., Salio, M., Galli, C., Norais, N., Rappuoli, R., Lanzavecchia, A. and Montecucco, C. (1998) Selective inhibition of Li-dependent antigen presentation by Helicobacter pylori toxin VacA. J. Exp. Med., 187, 135-140.
22. Montecucco, C., Papini, E., de Bernard, M. and Zoratti, M. (1999) Molecular and cellular activities of Helicobacter pylori pathogenic factors. FEBS Lett., 452, 16-21.
23. Papini, E., de Bernard, M., Milia, E., Zerial, M., Rappuoli, R. and Montecucco, C. (1994) Cellular vacuoles induced by Helicobacter pylori originate from late endosomal compartments. Proc. Natl Acad. Sci. USA, 91, 9720-9724
24. Papini, E., Satin, B., Bucci, C., de Bernard, M., Telford, J.L., Manetti, R., Rappuoli, R., Zerial, M. and Montecucco, C. (1997) The small GTP binding protein rab7 is essential for cellular vacuolation induced by Helicobacter pylori cytotoxin. EMBO J., 16, 15-24.
25. Parsonnet, J. (1998) Helicobacter pylori: the size of the problem. Gut Suppl. 1, 43, S6-S9.
26. Phadnis, S.H., Ilver, D., Janzon, L., Normark, S. and Westblom, T.U. (1994) Pathological significance and molecular characterization of the vacuolating toxin gene of Helicobacter pylori. Infect. Immun., 62, 1557-1565.
27. Ramaekers, F.C.S., Puts, J.J.G., Kant, A., Moesker, O., Jap, P.H.K. and Vooijs, G.P. (1982) Use of antibodies to intermediate filaments in the characterization of human tumors. Cold Spring Harb. Symp. Quant. Biol., 46, 331-339.
28. Ricci, V., Sommi, P., Fiocca, R., Romano, M., Solcia, E. and Ventura, U. (1997) Helicobacter pylori vacuolating toxin accumulates within the endosomal-vacuolar compartment of cultured gastric cells and potentiates the vacuolating activity of ammonia. J. Pathol., 183, 453-459.
29. Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
30. Satin, B., Norais, N., Telford, J.L., Rappuoli, R., Murgia, M., Montecucco, C. and Papini, E. (1997) Vacuolating toxin of Helicobacter pylori inhibits maturation of procathepsin D and degradation of epidermal growth factor in HeLa cells through a partial neutralization of acidic intracellular compartments. J. Biol. Chem., 272, 25022-25028.
31. Schmitt, W. and Haas, R. (1994) Genetic analysis of the Helicobacter pylori vacuolating cylotoxin: structural similarities with the IgA protease type of exported protein. Mol. Microbiol., 12, 307-319.
32. Serebriiskii, I., Khazak, V. and Golemis, E.A. (1999) A two-hybrid dual bait system to discriminate specificity of protein interactions. J. Biol. Chem., 274, 17080-17087.
33. Steinbock, F.A. and Wicke, G. (1999) Plectine: a cytolinker by design. Biol. Chem., 380, 151-158.
34. Steinert, P.M. and Roop, D.R. (1988) Molecular and cellular biology of intermediate filaments. Annu. Rev. Biochem., 57, 593-625.
35. Telford, J.L. et al. (1994) Gene structure of the Helicobacter pylori cytotoxin and evidence of its key role in gastric disease. J. Exp. Med., 179, 1653-1658.
36. Thomas, P.A., Kirschmann, D.A., Cerhan, J.R., Folberg, R., Seftor, E.A., Sellers, T.A. and Hendrix, M.J. (1999) Association between keratin and vimentin expression, malignant phenotype, and survival in postmenopausal breast cancer patients. Clin. Cancer Res., 5, 2698-2703.
37. Tombola, F. et al. (1999) Helicobacter pylori vacuolating toxin forms anion-selective channels in planar lipid bilayers: possible implications for the mechanism of cellular vacuolation. Biophys. J., 76, 1401-1409.
38. Vojtek, A.B., Hollenberg, S.M. and Cooper, J.A. (1993) Mammalian Ras interacts directly with the serine/threonine kinase Raf. Cell, 74, 205-214.
39. Warren, J.R and Marshall, B.J. (1983) Unidentified curved bacilli on gastric epithelium in active chronic gastritis. Lancet, 1, 1273-1275.
40. Ye, D., Willhite, D.C. and Blancke, S.R. (1999) Identification of the minimal intracellular vacuolating domain of the Helicobacter pylori vacuolating toxin. J. Biol. Chem., 274, 9277-9282.