Isolation and characterization of HRT1 using a genetic screen for mutants unable to degrade Gic2p in Saccharomyces cerevisiae

Genetics

Volumn 155, Issue 3, 2000, Pages 1033-1044

  Blondel, Marc ^a   Galan, Jean Marc ^a   Peter, Matthias ^a  

^a SWISS INST FOR EXP CANCER RESEARCH   (Switzerland)

Author keywords

[No Author keywords available]

Indexed keywords

FUNGAL PROTEIN; HOMEODOMAIN PROTEIN; PROTEIN GIC2P; PROTEIN HRT1; UNCLASSIFIED DRUG;

ALLELE; ARTICLE; CELL STRUCTURE; CELLULAR DISTRIBUTION; CONTROLLED STUDY; FUNGAL GENETICS; FUNGUS MUTANT; GENE FUNCTION; GENE ISOLATION; GENE MUTATION; NONHUMAN; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN DEGRADATION; PROTEIN DOMAIN; PROTEIN PROTEIN INTERACTION; PROTEIN TARGETING; SACCHAROMYCES CEREVISIAE; THERMOSTABILITY;

ALLELES; CARRIER PROTEINS; CELL CYCLE; CELL CYCLE PROTEINS; CELL NUCLEUS; CYCLINS; CYTOPLASM; F-BOX PROTEINS; FUNGAL PROTEINS; MUTAGENESIS, SITE-DIRECTED; MUTATION; PEPTIDE SYNTHASES; PROTEIN PROCESSING, POST-TRANSLATIONAL; RECOMBINANT PROTEINS; REPRESSOR PROTEINS; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS; SKP CULLIN F-BOX PROTEIN LIGASES; TEMPERATURE; TWO-HYBRID SYSTEM TECHNIQUES; UBIQUITIN-PROTEIN LIGASE COMPLEXES; UBIQUITIN-PROTEIN LIGASES; UBIQUITINS;

FUNGI; SACCHAROMYCES CEREVISIAE; SACCHAROMYCETALES;

EID: 0033927342     PISSN: 00166731     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (60)

1. AUSUBEL, F. M., R. BRENT, R. E. KINGSTON, D. D. MOORE, J. G. SEIDMAN et al., 1991 Current Protocols in Molecular Biology. Greene Publishing Associates and Wiley-Interscience, New York.
2. BACHMAIR, A., D. FINLEY and A. VARSHAVSKY, 1986 In vivo half-life of a protein is a function of its amino-terminal residue. Science 234: 179-186.
3. BAI, C., P. SEN, K. HOFMANN, L. MA, M. GOEBL et al., 1996 SKP1 connects cell cycle regulators to the ubiquitin proteolysis machinery through a novel motif, the F-box. Cell 86: 263-274.
4. BAILLY, V., S. LAUDER, S. PRAKASH and L. PRAKASH, 1997 Yeast DNA repair proteins Rad6 and Rad18 form a heterodimer that has ubiquitin conjugating, DNA binding, and ATP hydrolytic activities. J. Biol. Chem. 272: 23330-23365.
5. BARRAL, Y., S. JENTSCH and C. MANN, 1995 G1 cyclin turnover and nutrient uptake are controlled by a common pathway in yeast. Genes Dev. 9: 399-409.
6. BLONDEL, M., P. M. ALEPUZ, L. S. HUANG, S. SHAHAM, G. AMMERER et al., 1999 Nuclear export of Far1p in response to pheromones requires the export receptor Msu5p/Ste21p. Genes Dev. 13: 2281-2300.
7. BORDALLO, J., and D. H. WOLF, 1999 A RING-H2 finger motif is essential for the function of Der3/Hrd1 in endoplasmic reticulum associated protein degradation in the yeast Saccharomyces cerevisiae. FEBS Lett. 448: 244-248.
8. BREEDEN, L. I., 1997 Alpha-factor synchronization of budding yeast. Methods Enzymol. 283: 332-341.
9. BROWN, J. L., M. JAQUENOUD, M. P. GULLI, J. CHANT and M. PETER, 1997 Novel Cdc42-binding proteins Gic1 and Gic2 control cell polarity in yeast. Genes Dev. 11: 2972-2982.
10. CHEN, G. C., Y. J. KIM and C. S. CHAN, 1997 The Cdc42 GTPase-associated proteins Gic1 and Gic2 are required for polarized cell growth in Saccharomyces cerevisiae. Genes Dev. 11: 2958-2971.
11. CHOI, W. J., M. W. CLARK, J. X. CHEN and A. Y. JONG, 1990 The CDC4 gene product is associated with the yeast nuclear skeleton. Biochem. Biophys. Res. Commun. 172: 1324-1330.
12. DESHAIES, R. J., 1997 Phosphorylation and proteolysis: partners in the regulation of cell division in budding yeast. Curr. Opin. Genet. Dev. 7: 7-16.
13. DESHAIES, R. J., V. CHAU and M. KIRSCHNER, 1997 Ubiquitination of the G1 cyclin Cln2p by a Cdc34p-dependent pathway. EMBO J. 14: 303-312.
14. DRURY, L. S., G. PERKINS and J. F. DIFFLEY, 1997 The Cdc4/34/53 pathway targets Cdc6p for proteolysis in budding yeast. EMBO J. 16: 5966-5976.
15. EPSTEIN, C. B., and F. R. CROSS, 1992 CLB5: a novel B cyclin from budding yeast with a role in S phase. Genes Dev. 6: 1695-1706.
16. FELDMAN, R. M., C. C. CORRELL, K. B. KAPLAN and R. J. DESHAIES, 1997 A complex of Cdc4p, Skp1p, and Cdc53p/cullin catalyzes ubiquitination of the phosphorylated CDK inhibitor Sic1p. Cell 91: 221-230.
17. FLICK, J. S., and M. JOHNSTON, 1991 GRR1 of Saccharomyces cerevisiae is required for glucose repression and encodes a protein with leucine-rich repeats. Mol. Cell. Biol. 11: 5101-5112.
18. FREED, E., K. R. LACEY, P. HUIE, S. A. LYAPINA, R. J. DESHAIES et al., 1999 Components of an SCF ubiquitin ligase localize to the centrosome and regulate the centrosome duplication cycle. Genes Dev. 13: 2242-2257.
19. GALAN, J. M., and M. PETER, 1999 Ubiquitin-dependent degradation of multiple F-box proteins by an autocatalytic mechanism. Proc. Natl. Acad. Sci. USA 96: 9124-9129.
20. GARI, E., L. PIEDRAFITA, M. ALDEA and E. HERRERO, 1997 A set of vectors with a tetracyclin-regulatable promoter system for modulated gene expression in Saccharomyces cerevisiae. Yeast 13: 837-848.
21. GIETZ, R. D., R. H. SCHIESTL, A. R. WILLEMS and R. A. WOODS, 1995 Studies on the transformation of intact yeast cells by the LiAc/ SS-DNA/PEG procedure. Yeast 11: 355-360.
22. GOEBL, M. G., J. YOCHEM, S. JENTSCH, J. P. MCGRATH, A. VARSHAVSKY et al., 1988 The yeast cell cycle gene CDC34 encodes a ubiquitin-conjugating enzyme. Science 241: 1331-1335.
23. GSTAIGER, M., A. MARTI and W. KREK, 1999 Association of human SCF(SKP2) subunit p19(SKP1) with interphase centrosomes and mitotic spindle poles. Exp. Cell Res. 247: 554-562.
24. GUTHRIE, C., and G. R. FINK, 1991 Guide to Yeast Genetics and Molecular Biology. Academic Press Inc., San Diego.
25. GYURIS, J., E. GOLEMIS, H. CHERTKOV and R. BRENT, 1993 Cdi1, a human G1 and S phase protein phosphatase that associates with Cdk2. Cell 75: 791-803.
26. HENCHOZ, S., Y. CHI, B. CATARIN, I. HERSKOWITZ, R. J. DESHAIES et al., 1997 Phosphorylation- and ubiquitin-dependent degradation of the cyclin-dependent kinase inhibitor Far1p in budding yeast. Genes Dev. 11: 3046-3060.
27. HERSHKO, A., H. HELLER, S. ELIAS and A. CIECHANOVER, 1983 Components of ubiquitin-protein ligase system. Resolution, affinity purification, and role in protein breakdown. J. Biol. Chem. 258: 8206-8214.
28. HOCHSTRASSER, M., 1996 Ubiquitin-dependent protein degradation, pp. 405-139 in Annual Reviews of Genetics, edited by ANNUAL REVIEWS Inc., Palo Alto, CA.
29. HOFFMAN, C. S., and F. WINSTON, 1987 A ten-minute DNA preparation from yeast effciently releases autonomous plasmids for transformation of Escherichia coli. Gene 57: 267-272.
30. HONDA, R., H. TANAKA and H. YASUDA, 1997 Oncoprotein MDM2 is a ubiquitin ligase E3 for minor suppressor p53. FEBS Lett. 420: 25-27.
31. HUIBRIGISE, J. M., M. SCHEFENER, S. BEAUDENON and P. M. HOWLEY, 1995 A family of proteins structurally and functionally related to the E6-AP ubiquitin-protein ligase. Proc. Natl. Acad. Sci. USA 92: 2563-2567.
32. JAQUENOUD, M., M. P. GULLI, K. PETER and M. PETER, 1998 The Cdc42p effector Gic2p is targeted for ubiquitin-dependent degradation by the SCFGrr1 complex. EMBO J. 17: 5360-5373.
33. JOAZEIRO, C. A., S. S. WING, H. HUANG, J. D. LEVERSON, T. HUNTER et al., 1999 The tyrosine kinase negative regulator c-Cbl as a RING-type, E2-dependent ubiquitin-protein ligase. Science 286: 309-312.
34. KAISER, P., R. A. SIA, E. G. BARDES, D. J. LEW and S. I. REED, 1998 Cdc34 and the F-box protein Met30 are required for degradation of the Cdk-inhibitory kinase Swe1. Genes Dev. 12: 2587-2597.
35. KAMURA, T., D. M. KOEPP, M. N. CONRAD, D. SKOWYRA, R. J. MORELAND et al., 1999 Rbx1, a component of die VHL tumor suppressor complex and SCF ubiquitin ligase. Science 284: 657-661.
36. KISHI, T., and F. YAMAO, 1998 An essential function of Grr1 for the degradation of Cln2 is to act as a binding core that links Cln2 to Skp1. J. Cell Sci. 111: 3655-3661.
37. KITAGAWA, K., D. SKOWYRA, S. J. ELLEDGE, J. W. HARPER and P. HIETER, 1999 SGT1 encodes an essential component of the yeast kinetoehore assembly pathway and a novel subunit of the SCF ubiquitin ligase complex. Mol. Cell 4: 21-33.
38. LISZTWAN, J., G. IMBERT, C. WIRBELAUER, C. M. GSTAIGER and W. KREK, 1999 The von Hippel-Lindau tumor suppressor protein is a component of an E3 ubiquitin-protein ligase activity. Genes Dev. 13: 1822-1833.
39. LORICK, K. L., J. P. JENSEN, S. FANG, A. M. ONG, S. HATAKEYAMA et al., 1999 RING fingers mediate ubiquitin-conjugating enzyme (E2)-dependent ubiquitination. Proc. Natl. Acad. Sci. USA 96: 11364-11369.
40. MA, J., and M. PTASHNE, 1987 A new class of yeast transcriptional activators. Cell 51: 113-119.
41. MATHIAS, N., S. JOHNSON, B. BYERS and M. GOEBL, 1999 The abundance of cell cycle regulatory protein Cdc4p is controlled by interactions between its F box and Skp1p. Mol. Cell. Biol. 19: 1759-1767.
42. NUBER, U., S. SCHWARZ, P. KAISER, R. SCHNEIDER and M. SCHEFFNER, 1996 Cloning of human ubiquitin-conjugating enzymes UbcH6 and UbcH7 (E2-F1) and characterization of their interaction with E6-AP and RSP5. J. Biol. Chem. 271: 2795-2800.
43. OHTA, T., J. J. MICHEL, A. J. SCHOTTELIUS and Y. XIONG, 1999a ROC1, a homolog of APC11, represents a family of cullin partners with an associated ubiquitin ligase activity. Mol. Cell 3: 535-541.
44. OHTA, T., J. J. MICHEL and Y. XIONG, 1999B Association with cullin partners protects ROC proteins from proteasome-dependent degradation. Oncogene 18: 6758-6766.
45. PATTON, E. E., A. R. WILLEMS, D. SA, L. KURAS, D. THOMAS et al., 1998 Cdc53 is a scaffold protein for multiple Cdc34/Skp1/ F-box protein complexes that regulate cell division and methionine biosynthesis in yeast. Genes Dev. 12: 692-705.
46. PETERS, J. M., 1998 SCF and APC: the yin and yang of cell cycle regulated proteolysis. Curr. Opin. Cell Biol. 10: 759-768.
47. Met30 complex. EMBO J. 19: 282-294.
48. SAURIN, A. J., K. L. BORDEN, M. N. BODDY and P. S. FREEMONT, 1996 Does this have a familiar RING? Trends Biochem. Sci. 21: 208-214.
49. SCHWOB, E., T. BOHM, M. D. MENDENHALL and K. NASMYTH, 1991 The B-type cyclin kinase inhibitor p40SIC1 controls the G1 to S transition in S. cerevisiae. Cell 79: 233-244.
50. SEOL, J. H., R. M. FELDMAN, W. ZACHARIAE, A. SHEVCHENKO, C. C. CORRELL, et al., 1999 Cdc53/cullin and the essential Hrt1 RING-112 subunit of SCF define a ubiquitin ligase module that activates the E2 enzymc Cdr34. Genes Dev. 13: 1614-1626.
51. SIKORSKI, R. S., and P. HIETER, 1989 A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics 122: 19-27.
52. SKOWYRA, D., K. L. CRAIG., M. TYERS, S. J. ELLEDGE and J. W. HARPER, 1997 F-box proteins are receptors that recruit phosphorylated substrates to the SCF ubiquitin-ligase complex. Cell 91: 209-219.
53. SKOWYRA, D., D. M. KOEPP, T. KAMURA, M. N. CONRAD, R. C. CONAWAY et al., 1999 Reconstitution of G1 cyclin ubiquitination with complexes containing SCFGrr1 and Rbx1. Science 284: 662-665.
54. STEBBINS, C. E., W. G. KAELIN, JR. and N. P. PAVLETICH, 1999 Structure of the VHL-ElonginC-ElonginB complex: implications for VHL tumor suppressor function. Science 284: 455-461.
55. VARSHAVSKY, A., 1996 The N-end rule: functions, mysteries, uses. Proc. Natl. Acad. Sci. USA 93: 12142-12149.
56. WANG, G., J. YANG and J. M. HUIBREGTSE, 1999 Functional domains of the Rsp5 ubiquitin-protein ligase. Mol. Cell. Biol. 19: 342-352.
57. WATERMAN, H., G. LEVKOWITZ, I. ALROY and V. YARDEN, 1999 The RING finger of c-Cbl mediates desensitization of the epidermal growth fact of receptor. J. Biol. Chem. 274: 22151-22154.
58. XIE, Y., and A. VARSHAVSKY, 1999 The E2-E3 interaction in the N-end rule pathway: the RING-H2 finger of E3 is required for the synthesis of multiubiquitin chain. EMBO J. 18: 6832-6844.
59. ZACHARIAE, W., A. SHEVCHENKO, P. D. ANREWS, R. CIOSK, M. J. STARK et al., 1998 Mass spectrometric analysis of the anaphase-promoting complex from yeast: identification of a subunit related to cullins. Science 279: 1216-1219.
60. ZHOU, P., and P. M. HOWLEY, 1998 Ubiquitination and degradation of the substrate recognition snbunits of SCF ubiquitin-protein ligases. Mol. Cell 2: 571-580.