Feedback-regulated degradation of the transcriptional activator Met4 is triggered by the SCF(Met30) complex

EMBO Journal

Volumn 19, Issue 2, 2000, Pages 282-294

  Rouillon, Astrid ^c   Barbey, Régine ^c   Patton, E Elizabeth ^a,b   Tyers, Mike ^a   Thomas, Dominique ^c  

^a MOUNT SINAI HOSPITAL   (Canada)

^b UNIVERSITY OF TORONTO   (Canada)

^c CNRS   (France)

Author keywords

Protein degradation; SCF ubiquitin ligase; Sulfur amino acid metabolism; Transcriptional repression

Indexed keywords

PROTEASOME; PROTEIN MET30; PROTEIN MET4; S ADENOSYLMETHIONINE; SULFUR AMINO ACID; TRANSCRIPTION FACTOR; UBIQUITIN PROTEIN LIGASE; UNCLASSIFIED DRUG;

AMINO ACID METABOLISM; ARTICLE; CELL CYCLE; CONTROLLED STUDY; FEEDBACK SYSTEM; GENETIC TRANSCRIPTION; NONHUMAN; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN LOCALIZATION; PROTEIN SYNTHESIS; SACCHAROMYCES CEREVISIAE; TRANSCRIPTION REGULATION;

SACCHAROMYCES CEREVISIAE;

EID: 0034677224     PISSN: 02614189     EISSN: None     Source Type: Journal    
DOI: 10.1093/emboj/19.2.282     Document Type: Article

References (49)

1. Bai, C., Sen, P., Hofmann, K., Ma, L., Goebl, M., Harper, J.W. and Elledge, S.J. (1996) SKP1 connects cell cycle regulators to the ubiquitin proteoiysis machinery through a novel motif, the F-box. Cell. 86, 263-274.
2. Blaiseau, PL. and Thomas,D. (1998) Multiple transcription activation complexes tether the yeast activator Met4 to DNA. EMBO J., 17, 6327-6336.
3. Chérest, H. and Surdin-Kerjan, Y. (1992) Genetic analysis of a new mutation conferring cysteine auxotrophy in Saccharomyces cerevisiae: updating of the sulfur metabolism pathway. Genetics, 130, 51-58.
4. Cormack, B.P., Valdivia, R.H. and Falkow, S. (1996) FACS-optimized mutants of the green fluorescent protein (GFP). Gene, 173, 33-38.
5. Feldman, R.M.R., Correll, C.C., Kaplan, K.B. and Deshaies, R.J. (1997) A complex of Cdc4p, Skp1p and Cdc53p/cullin catalyzes ubiquitination of the phosphorylated CDK inhibitor Sic1p. Cell, 91, 221-230.
6. 2/metaphase. Nature, 366, 358-362.
7. Gietz, D., St. Jean, A., Woods, R.A. and Schiestl, R.H. (1992) Improved method for high efficiency transformation of intact yeast cells. Nucleic Acids Res., 20, 1425-1426.
8. Gorner, W., Durchschlag, E., Martinez-Pastor, M.T., Estruch, F., Ammerer, G., Hamilton, B., Ruis, H. and Schuller, C. (1998) Nuclear localization of the C2H2 zinc finger protein Msn2p is regulated by stress and protein kinase A activity. Genes Dev., 12, 586-597.
9. Harper, J.W. and Elledge, S.J. (1999) Skipping into the E2F1-destruction pathway. Nature Cell Biol., 1, E5-E7.
10. Hershko, A., Heller, H., Elias, S. and Ciechanover, A. (1983) Components of ubiquitin-protein ligase system. Resolution, affinity purification and role in protein breakdown. J. Biol. Chem., 258, 8206-8214.
11. Hochstrasser, M. (1996) Ubiquitin-dependent protein degradation. Annu. Rev. Genet., 30, 405-439.
12. Hodgson, C.P. and Fisk, R.Z. (1987) Hybridization probe size control: optimized 'oligolabelling'. Nucleic Acids Res., 15, 6295.
13. Johnson, P.R., Swanson, R., Rakhilina, L. and Hochstrasser, M. (1998) Degradation signal masking by heterodimerization of MATα2 and MATa1 blocks their mutual destruction by the ubiquitin-proteasome pathway. Cell, 94, 217-227.
14. Kaiser, P., Sia, R.A., Bardes, E.G., Lew, D.J. and Reed, S.I. (1998) Cdc34 and the F-box protein Met30 are required for degradation of the Cdk-inhibitory kinase Swe1. Genes Dev., 12, 2587-2597.
15. Kaplan, K.B., Hyman, A.A. and Sorger, P.K. (1997) Regulating the yeast kinetochore by ubiquitin-dependent degradation and Skp1p-mediated phosphorylation. Cell, 91, 491-500.
16. Koegl, M., Hoppe, T., Schlenker, S., Ulrich, H.D., Mayer, T.U. and Jentsch, S. (1999) A novel ubiquitination factor, E4, is involved in multiubiquitin chain assembly. Cell, 96, 635-644.
17. Koepp, D.M., Harper, J.W. and Elledge, S.J. (1999) How the cyclin became a cyclin: regulated proteolysis in the cell cycle. Cell, 97, 431-434.
18. Komeili, A. and O'Shea, E.K. (1999) Roles of phosphorylation sites in regulating activity of the transcription factor Pho4. Science, 284, 977-980.
19. Kuras, L., Barbey, R. and Thomas, D. (1997) Assembly of a bZIP/bHLH transcription activation complex: formation of the yeast Cbf1-Met4-Met28 complex is regulated through Met28 stimulation of Cbf1 DNA binding. EMBO J., 16, 2441-2451.
20. Kuras, L. and Thomas, D. (1995) Functional analysis of Met4, a yeast transcriptional activator responsive to S-adenosylmethionine. Mol. Cell. Biol., 15, 208-216.
21. Lammer, D., Mathias, N., Laplaza, J.M., Jiang, W., Liu, Y., Callis, J., Goebl, M. and Estelle, M. (1998) Modification of yeast Cdc53p by the ubiquitin-related protein rub1p affects function of the SCFCdc4 complex. Genes Dev., 12, 914-926.
22. 1 cyclin C1n2 induced by CDK-dependent phosphorylation. Science, 271, 1597-1601.
23. Li, F.N. and Johnston, M. (1997) Grr1 of Saccharomycex cerevisiae is connected to the ubiquitin proteolysis machinery through Skp1: coupling glucose sensing to gene expression and the cell cycle. EMBO J., 16, 5629-5638.
24. Liakopoulos, D., Doenges, G., Matuschewski, K. and Jentsch, S. (1998) A novel protein modification pathway related to the ubiquitin system. EMBO J., 17, 2208-2214.
25. Mathias, N., Johnson, S., Byers, B. and Goebl, M. (1999) The abundance of cell cycle regulatory protein Cdc4p is controlled by interactions between its F box and Skp1p. Mol. Cell. Biol., 19, 1759-1767.
26. Mitchell, D.A., Marshall, T.K. and Deschenes, R.J. (1993) Vectors for the inducible overexpression of glutathione S-transferase fusion proteins in yeast. Yeast, 9, 715-722.
27. Nehlin, J. and Ronne, H. (1990) Yeast Mig1 repressor is related to the mammalian early growth response and Wilm's tumor finger protein. EMBO J., 9, 2891-2898.
28. Pahl, H.L. and Baeuerle, P.A. (1996) Control of gene expression by proteolysis. Curr. Opin. Cell Biol., 8, 340-347.
29. Patton, E.E., Willems, A.R., Sa, D., Kuras, L., Thomas, D., Craig, K.L. and Tyers, M. (1998a) Cdc53 is a scaffold protein for multiple Cdc34/Skp1/ F-box protein complexes that regulate cell division and methionine biosynthesis in yeast. Genex Dev., 12, 692-705.
30. Patton, E.E., Willems, A.R. and Tyers, M. (1998b) Combinatorial control in ubiquitin-dependent proteolysis: don't Skp the F-box hypothesis. Trends Genet., 14, 236-243.
31. Patton, E.E., Peyraud, C., Rouillon, A., Surdin-Kerjan, Y., Tyers, M. and Thomas, D. (1999) manuscript in preparation.
32. Russelu, I.D., Grancell, A.S. and Sorger, P.K. (1999) The unstable F-box protein p58-Ctf13 forms the structural core of the CBF3 kinetochore complex. J. Cell Biol., 145, 933-950.
33. Schmitt, M., Brown, T. and Trumpower, B. (1990) A rapid and simple method for preparation of RNA from Saccharomyces cerevisiae. Nucleic Acids Res., 18, 3091-3092.
34. Seol, J.H. et al. (1999) Cdc53/cullin and the essential hrt1 RING-H2 subunit of SCF define a ubiquitin ligase module that activates the E2 enzyme cdc34. Genes Dev., 13, 1614-1626.
35. Sherman, F., Fink, G.R. and Hicks, J.B. (1979) Methods in Yeast Genetics: A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
36. Sikorski, R.S. and Hieter, P. (1989) A system of shuttle vectors and yeast strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics, 122, 19-27.
37. Skowyra, D., Craig, K.L., Tyers, M., Elledge, S.J. and Harper, J.W. (1997) F-box proteins are receptors that recruit phosphorylated substrates to the SCF ubiquitin-ligase complex. Cell, 91, 209-219.
38. Skowyra, D., Koepp, D.M., Kamura, T., Conrad M.N., Conaway, R.C, Conaway, J.W., Elledge, S.J. and Harper, J.W. (1999) Reconstitution of G1 cyclin ubiquitination with complexes containing SCFGrr1 and Rbx1. Science, 284, 662-665.
39. Solow, S.P., Lezina, L. and Lieberman, P.M. (1999) Phosphorylation of TFIIA stimulates TATA binding protein-TATA interaction and contributes to maximal transcription and viability in yeast. Mol. Cell. Biol., 19, 2846-2852.
40. Spellman, P.T., Sherlock, G., Zhang, M.Q., Iyer, V.R., Anders, K., Eisen, M.B., Brown, P.O., Botstein, D. and Futcher, B. (1998) Comprehensive identification of cell cycle-regulated genes of the yeast Saccharomyces cerevisiae by microarray hybridization. Mol. Biol. Cell, 9, 3273-3297.
41. Tavazoie, S., Hughes, J.D., Campbell, M.J., Cho, R.J. and Church, G.M. (1999) Systematic determination of genetic network architecture. Nature Genet., 22, 281-285.
42. Teng-umnuay, P., Morris, H.R., Dell, A., Panico, M., Paxton, T. and West, C.M. (1998) The cytoplasmic F-box binding protein SKP1 contains a novel pentasaccharide linked to hydroxyproline in Dictyostelium. J. Biol. Chum., 273, 18242-18249.
43. Thomas, D. and Surdin-Kerjan, Y. (1997) Metabolism of sulfur amino acids in Saccharomyces cerevisiae. Microbiol. Mol. Biol. Rev., 61, 503-532.
44. Thomas, D., Rothstein, R., Rosenberg, N. and Surdin-Kerjan, Y. (1988) SAM2 encodes the second methionine S-adenosyl transferase in Saccharomyces cerevisiae: physiology and regulation of both enzymes. Mol. Cell. Biol., 8, 5132-5139.
45. Thomas, D., Kuras, L., Barbey, R., Cherest, H., Blaiseau, P.L. and Surdin-Kerjan, Y. (1995) Met30, a yeast transcriptional inhibitor that responds to S-adenosylmethionine, is an essential protein with WD40 repeats. Mol. Cell Biol., 15, 6526-6534.
46. Thomas, P.S. (1980) Hybridization of denatured DNA and small DNA fragments transferred to nitrocellulose. Proc. Natl Acad. Sci. USA, 77, 5201-5205.
47. Willems, A.R., Lanker, S., Patton, E.E., Craig, K.L., Nason, T.F., Mathias, N., Kobayashi, R., Wittenberg, C. and Tyers, M. (1996) Cdc53 targets phosphorylated G1 cyclins for degradation by the ubiquitin proteolytic pathway. Cell, 86, 453-463.
48. Winston, J.T., Strack, P., Beer-Romero, P., Chu, C.Y., Elledge, S.J. and Harper, J.W. (1999) The SCFβ-TRCP-ubiquitin ligase complex associates specifically with phosphorylated destruction motifs in IKBα and ß-catenin and stimulates IKBα ubiquitination in vitro. Genes Dev., 13, 270-283.
49. Zhou, P. and Howley, P.M. (1998) Ubiquitination and degradation of the substrate recognition subunits of SCF ubiquitin-protein ligases. Mol. Cell, 2, 571-580.