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1 cyclins.
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of special interest. Repeated rounds of DNA replication that occur upon depletion of CDC13 (S. pombe cyclin B) require the CIG1 and CIG2 cyclins. S phase is postponed in cig1 cig2 cells until CDC13 begins to accumulate, and CDC13-depleted cig1 cig2 cells are unable to replicate DNA, suggesting that the mitotic cyclin CDC13 promotes both DNA synthesis and mitosis in the absence of CIG1 and CIG2 function. It is proposed that low levels of CDC13/CDK activity trigger S phase in cig1 cig2 cells, whereas high levels are required to trigger mitosis. It remains unclear whether other unidentified cyclins collaborate with CDC13 to effect S and M phase.
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1 cyclins. of special interest EMBO J. 15:1996;850-860 Repeated rounds of DNA replication that occur upon depletion of CDC13 (S. pombe cyclin B) require the CIG1 and CIG2 cyclins. S phase is postponed in cig1 cig2 cells until CDC13 begins to accumulate, and CDC13-depleted cig1 cig2 cells are unable to replicate DNA, suggesting that the mitotic cyclin CDC13 promotes both DNA synthesis and mitosis in the absence of CIG1 and CIG2 function. It is proposed that low levels of CDC13/CDK activity trigger S phase in cig1 cig2 cells, whereas high levels are required to trigger mitosis. It remains unclear whether other unidentified cyclins collaborate with CDC13 to effect S and M phase.
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1 cyclins for degradation by the ubiquitin proteolytic pathway
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of special interest. CDC53 is a tightly-bound subunit of CLN2/CDC28 complexes. CDC53 also binds the ubiquitin-conjugating enzyme CDC34 and like CDC34 is required for rapid proteolysis of phosphorylated CLN2. CDC53 associates with phosphorylated CLN2, which is unstable, but not with unmodified CLN2, which is stable. Taken together, these results suggest that CDC53 targets phosphorylated CLN2 for CDC34-dependent ubiquitination.
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