Biosynthesis of a head-to-tail cyclized protein with improved biological activity

Journal of the American Chemical Society

Volumn 121, Issue 23, 1999, Pages 5597-5598

  Camarero, Julio A ^a   Muir, Tom W ^a  

^a ROCKEFELLER UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

PROTEIN;

ARTICLE; CARBOXY TERMINAL SEQUENCE; CYCLIZATION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; PROTEIN DOMAIN; PROTEIN STRUCTURE; PROTEIN SYNTHESIS; REACTION ANALYSIS;

EID: 0033575092     PISSN: 00027863     EISSN: None     Source Type: Journal    
DOI: 10.1021/ja990929n     Document Type: Article

References (33)

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28. Circular protein (3) was characterized by ESMS [observed mass = 6747.3 ± 1.3 Da, expected (average isotopic composition) = 6747.6 Da] and tryptic digestion which afforded a peptide map consistent with a head-to-tail circular structure. Approximately 0.5 mg of purified (3) was obtained from 1 L of bacterial growth.
29. The linear c-Crk N-terminal SH3 domain was obtained using standard recombinant expression techniques.
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31. An increase in binding affinity was also recently observed for a circular version of a WW protein domain: see ref 2b.
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