Construction and use of low-copy number T7 expression vectors for purification of problem proteins: Purification of Mycobacterium tuberculosis RmlD and Pseudomonas aeruginosa LasI and RhlI proteins, and functional analysis of purified RhlI

Gene

Volumn 237, Issue 2, 1999, Pages 361-371

  Hoang, Tung T ^a   Ma, Yufang ^a   Stern, Richard J ^a   McNeil, Michael R ^a   Schweizer, Herbert P ^a  

^a Department of Environmental and Radiological Health Sciences   (United States)

Author keywords

Autoinducer; Inclusion bodies; Overexpression; Protein purification

Indexed keywords

BACTERIAL PROTEIN; CARRIER PROTEIN; CHITIN; COENZYME A; HISTIDINE DERIVATIVE; HYBRID PROTEIN; LACTONE DERIVATIVE; OXIDOREDUCTASE; PROTEINASE; RECOMBINANT PROTEIN; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE; S ADENOSYLMETHIONINE;

ARTICLE; CELL INCLUSION; DNA REPLICATION ORIGIN; ENZYME ACTIVITY; ESCHERICHIA COLI; EXPRESSION VECTOR; GENE CONSTRUCT; GENE OVEREXPRESSION; MYCOBACTERIUM TUBERCULOSIS; NONHUMAN; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN PURIFICATION; PSEUDOMONAS AERUGINOSA;

BACTERIAL PROTEINS; BACTERIOPHAGE T7; CLONING, MOLECULAR; GENE EXPRESSION; GENETIC VECTORS; HISTIDINE; MYCOBACTERIUM TUBERCULOSIS; PROMOTER REGIONS (GENETICS); PSEUDOMONAS AERUGINOSA; RECOMBINANT FUSION PROTEINS;

EID: 0032882026     PISSN: 03781119     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1119(99)00331-5     Document Type: Article

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