Overexpression of recombinant proteins with a C-terminal thiocarboxylate: Implications for protein semisynthesis and thiamin biosynthesis

Protein Science

Volumn 7, Issue 8, 1998, Pages 1839-1842

  Kinsland, Cynthia ^a   Taylor, Sean V ^a   Kelleher, Neil L ^a   McLafferty, Fred W ^a   Begley, Tadhg P ^a  

^a Department of Obstetrics Gynecology   (United States)

Author keywords

C terminal thiocarboxylate; Protein semisynthesis; Recombinant proteins; Thiamin biosynthesis

Indexed keywords

CARBOXYLIC ACID DERIVATIVE; CHITIN; DNA; RECOMBINANT PROTEIN; THIAMINE;

ARTICLE; FOURIER ANALYSIS; GENE OVEREXPRESSION; MASS SPECTROMETRY; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN PROCESSING; PROTEIN PROTEIN INTERACTION; PROTEIN SYNTHESIS;

EID: 0032464350     PISSN: 09618368     EISSN: None     Source Type: Journal    
DOI: 10.1002/pro.5560070821     Document Type: Article

References (18)

1. Ausubel FM, Brent R, Kingston RE, Moore DD, Seidman JG, Smith JA, Struhl K, eds. 1987. Current protocols in molecular biology. New York: John Wiley & Sons.
2. Barany G, Merrifield RB. 1979. Solid-phase peptide synthesis. In: Gross E, Meienhofer J, eds. The peptides, vol. 2. New York: Academic Press, pp 1-284.
3. Begley TP. 1996. The biosynthesis and degradation of thiamin (vitamin B1) (January 1986 to January 1996). Natural Product Rep 13:177-186.
4. Chong S, Mersha FB, Comb DC, Scott ME, Landry D, Vence LM, Perler FB, Benner J, Kucera RB, Hirvonen CA, Pelletier JJ, Paulus H, Xu MQ. 1997. Single-column purification of free recombinant proteins using a self-cleavable affinity tag derived from a protein splicing element. Gene (Amsterdam) 192:271-281.
5. Liu C-F, Rao C, Tam JP. 1996. Acyl disulfide-mediated intramolecular acylation for orthogonal coupling between unprotected peptide segments. Mechanism and application. Tetrahedron Lett 37:933-936.
6. McLafferty FW. 1994. High-resolution tandem FT mass spectrometry above 10 kDa. Acc Chem Res 27:379-386.
7. Muir TW, Dawson PE, Kent SBH. 1997. Protein synthesis by chemical ligation of unprotected peptides in aqueous solution. In: Fields GB, ed. Methods in enzymology, vol. 289. San Diego: Academic Press, Inc. pp 266-298.
8. Perler F, Xu MQ, Paulus H. 1997. Protein splicing and autoproteolysis mechanisms. Curr Opin Chem Biol 1:292-299.
9. Sambrook J, Fritsch EF, Maniatis T. 1989. Molecular cloning: A laboratory manual. Plainview, NY: Cold Spring Harbor Laboratory Press.
10. Schägger H, von Jagow G. 1987. Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa. Anal Biochem 166:368-379.
11. Shao Y, Paulus H. 1997. Protein splicing: Estimation of the rate of O-N and S-N acyl rearrangements, the last step of the splicing process. J Peptide Res 50:193-198.
12. Shao Y, Xu MQ, Paulus H. 1995. Protein splicing: Characterization of the aminosuccinimide residue at the carboxyl terminus of the excised intervening sequence. Biochemistry 34:10844-10850.
13. Tam JP, Lu Y-A, Liu C-F, Shao J. 1995. Peptide synthesis using unprotected peptides through orthogonal coupling methods. Proc Natl Acad Sci USA 92:12485-12489.
14. Taylor SV, Kelleher NL, Kinsland C, Chia H-J, Costello CA, Backstrom AD, McLafferty FW, Begley TP. 1998. Identification of ThiS thiocarboxylate as the immediate sulfur donor in the thiazole formation. J Biol Chem 273:16555-16560.
15. Vander Horn PB, Backstrom AD, Stewart V, Begley TP. 1993. Structural genes for thiamine biosynthetic enzymes (thiCEFGH) in Escherichia coli K-12. J Bacterial 175:982-992.
16. Wilm M, Mann M. 1996. Analytical properties of the nanoelectrospray ion source. Anal Chem 68:1-8.
17. Xu MQ, Comb DG, Paulus H, Noren CJ, Shao Y, Perler FB. 1994. Protein splicing: An analysis of the branched intermediate and its resolution by succinimide formation. EMBO J 13:5517-5522.
18. Zhang L, Tam JP. 1997. Orthogonal coupling of unprotected peptide segments through histidyl amino terminus. Tetrahedron Lett 38:3-6.