Plants as bioreactors for protein production: Avoiding the problem of transgene silencing

Plant Molecular Biology

Volumn 43, Issue 2-3, 2000, Pages 347-359

  De Wilde, C ^a   Van Houdt, H ^a   De Buck, S ^a   Angenon, G ^a   De Jaeger, G ^a   Depicker, A ^a  

^a DEPARTMENT OF PLANT SYSTEMS BIOLOGY   (Belgium)

Author keywords

Heterologous protein production; Instability of accumulation levels; Plants as protein factories; Transgene silencing

Indexed keywords

GENE DELETION; GENETIC STABILITY; PROTEIN PRODUCTION; TRANSGENE; TRANSGENIC PLANT;

EMBRYOPHYTA;

EID: 0033824414     PISSN: 01674412     EISSN: None     Source Type: Journal    
DOI: 10.1023/a:1006464304199     Document Type: Article

References (126)

1. Site-specific integration of DNA into wild-type and mutant lox sites placed in the plant genome
2. Efficacy of a food plant-based oral cholera toxin B subunit vaccine
3. High-tech herbal medicine: Plant-based vaccines
4. Secretion of a heat-stable fungal β-glucanase from transgenic, suspension-cultured barley cells
5. Direct gene transfer: Chemical, electrical and physical methds
6. Instability of transgene expression in field grown tobacco carrying the csr1-1 gene for sulfonylurea herbicide resistance
7. Characterization of a plant scaffold attachment region in a DNA fragment that normalizes transgene expression in tobacco
8. A trout growth hormone is expressed, correctly folded and partially glycosylated in the leaves but not the seeds of transgenic plants
9. Details of T-DNA structural organization from a transgenic petunia population exhibiting co-suppression
10. Environmental impact of transgenic plants
11. Two T-DNAs co-transformed into Brassica napus by a double Agrobacterium tumefaciens infection are mainly integrated at the same locus
12. The DNA sequences of T-DNA junctions suggest that complex T-DNA loci are formed by a recombination process resembling T-DNA integration
13. Vector backbone sequences are frequently integrated into the genome of transgenic plants obtained by Agrobacterium transformation
14. Suppression of β-1,3-glucanase transgene expression in homozygous plants
15. Assembly of an antibody and its derived antibody fragment in Nicotiana and Arabidopsis
16. T-DNA integration patterns in co-transformed plant cells suggest that T-DNA repeats originate from ligation of separate T-DNAs
17. Screening for transgenic lines with stable and suitable accumulation levels of a heterologous protein
18. Gene silencing results in instability of antibody production in transgenic plants
19. Production of antibodies in transgenic plants: A general introduction
20. Identification of plant genetic loci involved in a posttranscriptional mechanism for meiotically reversible transgene silencing
21. Post-transcriptional gene silencing in plants
22. Post-transcriptional reporter transgene silencing in transgenic tobacco
23. Analysis of the T-DNA structure in a large number of transgenic petunias generated by Agrobacterium-mediated transformation
24. Co-suppression of nitrate reductase host genes and transgenes in transgenic tobacco plants
25. Expression of active human interferon-β in transgenic plants
26. Expression of single copies of a strongly expressed 35S transgene can be silenced post-transcriptionally
27. Arabidopsis mutants impaired in Co-suppression
28. Transgene inactivation in Petunia hybrida is influenced by the properties of the foreign gene
29. Suppression of virus accumulation in transgenic plants exhibiting silencing of nuclear genes
30. Expression of single-chain antibody fragments (scFv) specific for beet necrotic yellow vein virus coat protein or 25 kDa protein in Escherichia coli and Nicotiana benthamiana
31. Transgenic plants: A low cost production system for recombinant antibodies
32. Transgene inactivation: Plants fight back!
33. Gene silencing and homology-dependent gene silencing in Arabidopsis: Genetic modifiers and DNA methylation
34. Controlling gene expression in transgenics
35. Repeat-induced gene silencing in mammals
36. The introduction and expression of transgenes in plants
37. Agrobacterium-mediated plant transformation: A scientifically intriguing story with significant applications
38. Plants as bioreactors
39. The arccelin-5 gene of Phaseolus vulgaris directs high seed-specific expression in transgenic Phaseolus acutifolius and Arabidopsis plants
40. Single-copy T-DNA insertions in Arobidopsis are the predominant form of integration in root-derived transgenics, whereas multiple insertions are found in leaf discs
41. A simple method to enrich an Agrobacterium-transformed population for plants containing only T-DNA sequences
42. Regulated inactivation of homologous gene expression in transgenic Nicotiana sylvestris plants containing a defense-related tobacco chitinase gene
43. Molecular farming: Using transgenic plants to produce novel proteins and other chemicals
44. A thermostable xylanase from Clostridium thermocellum expressed at high levels in the apoplast of transgenic tobacco has no detrimental effects and is easily purified
45. Production of antibodies in transgenic plants
46. Plant-based production of xenogenic proteins
47. Commercial production of avidin from transgenic maize: Characterization of transformant, production, processing, extraction and purification
48. Transgene copy number can be positively or negatively associated with transgene expression
49. Nuclear matrix attachment regions and plant gene expression
50. Molecular and cytogenetic analyses of stably and unstably expressed transgene loci in tobacco
51. Molecular and cytogenetic characterization of a transgene locus that induces silencing and methylation of homologous promoters in trans
52. Developmental significance of epigenetic impositions on the plant genome: A paragenetic function for chromosomes
53. Co-suppression, flower color patterns, and metastable gent expression states
54. T-DNA is organized predominantly in inverted repeat structures in plants transformed with Agrobactetium tumefaciens C58 derivatives
55. Chalcone synthase co-suppression phenotypes in petunia flowers: Comparison of sense vs. antisense constructs and single-copy vs. complex T-DNA sequences
56. Production of a diagnostic monoclonal antibody in perennial alfalfa plants
57. Promoter methylation and progressive transgene inactivation in Arabidopsis
58. Homology recognition during T-DNA integration into the plant genome
59. Integration of T-DNA binary vector 'backbone' sequences into the tobacco genome: Evidence for multiple complex patterns of integration
60. Listening to the silent genes: Transgene silencing, gene regulation and pathogen control
61. Production of recombinant proteins in transgenic plants: Practical considerations
62. Plant seed oil-bodies as an immobilization matrix for a recombinant xylanase from the rumen fungus Neocallimastix patriciarum
63. Generation and assembly of secretory antibodies in plants
64. Characterization of a recombinant plant monoclonal secretory antibody and preventive immunotherapy in humans
65. Secretion of biologically active human interleukin-2 and interleukin-4 from genetically modified tobacco cells in suspension culture
66. On defining T-DNA
67. Transgenic plants as vaccine production systems
68. Edible vaccine protects mice against Escherichia coli heat-labile enterotoxin (LT): Potatoes expressing a synthetic LT-B gene
69. Characterization of a human glycoprotein (erythropoietin) produced in cultured tobacco cells
70. Position effects and epigenetic silencing of plant transgenes
71. Reversible methylation and inactivation of marker genes in sequentially transformed tobacco plants
72. Homology-dependent gene silencing in transgenic plants: Epistatic silencing loci contain multiple copies of methylated transgenes
73. RNA-mediated RNA degradation and chalcone synthase A silencing in Petunia
74. Stabilities and instabilities in transgene expression
75. Epigenetic variants of a transgenic petunia line show hypermethylation in transgene DNA: An indication for specific recognition of foreign DNA in transgenic plants
76. Homology-dependent gene silencing in plants
77. Endogenous and environmental factors influence 35S promoter methylation of a maize A1 gene construct in transgenic Petunia and its colour phenotype
78. Differences in DNA-methylation are associated with a paramutation phenomenon in transgenic Petunia
79. Release of epigenetic gene silencing by trans-acting mutations in Arabidopsis
80. Approaching the lower limits of transgene variability
81. Exploring transgenic plants as a new vaccine source
82. Subcellular targeting and purification of recombinant proteins in plant production systems
83. Silencing of an aleurone-specific gene in transgenic rice is caused by a rearranged transgene
84. Introduction of a chimeric chalcone synthase gene into Petunia results in reversible co-suppression of homologous genes in trans
85. Field trial analysis of nitrate reductase co-suppression: A comparative study of 38 combinations of transgene loci
86. Frequencies, timing, and spatial patterns of co-suppression of nitrate reductase and nitrite reductase in transgenic tobacco plants
87. Systemic acquired silencing: Transgene-specific post-transcriptional silencing is transmitted by grafting from silenced stock to non-silenced scions
88. Production of biologically active hirudin in plant seeds using oleosin partitioning
89. Transgene expression variability (poSition effect) of CAT and GUS reporter genes driven by linked divergent T-DNA promoters
90. Production of active Bacillus licheniformis α-amylase in tobacco and its application in starch liquefaction
91. Phytasecontaining transgenic seeds as a novel feed additive for improved phosphorus utilization
92. Expression of a hybrid (1-3,1-4)-β-glucanase in barley protoplasts
93. The frequency and degree of co-suppression by sense chalcone synthase transgenes are dependent on transgene promoter strength and are reduced by premature nonsense codons in the transgene coding sequence
94. Transfer of non-TDNA portions of the Agrobacterium tumefaciens Ti plasmid pTiA6 from the left terminus of T(L)-DNA
95. Compositional compartmentalization and compositional patterns in the nuclear genomes of plants
96. Gene silencing: Repeats that count
97. RNA-mediated virus resistance: Role of repeated transgenes and delineation of targeted regions
98. Gene silencing: Plants and viruses fight it out
99. Single-copy transgenic wheat generated through the resolution of complex integration patterns
100. The silence of genes in transgenic plants
101. Post-transcriptional silencing of chalcone synthase in Petunia by inverted transgene repeats
102. Position-dependent methylation and transcriptional silencing of transgenes in inverted T-DNA repeats: Implications for posttranscriptional silencing of homologous host genes in plants
103. Immunogenicity in humans of a recombinant bacterial antigen delivered in a transgenic potato
104. Treatment with propionic and butyric acid enhances expression variegation and promoter methylation in plant transgenes
105. Tetracycline-dependent activation of an upstream promoter reveals transcriptional interference between tandem genes within T-DNA in tomato
106. Deviating T-DNA transfer from Agrobacterium tumefaciens to plants
107. Flavonoid genes in Petunia: Addition of a limited number of gene copies may lead to a suppression of gene expression
108. Coordinate expression of antibody subunit genes yields high levels of functional antibodies in roots of transgenic tobacco
109. Post-transcriptional silencing of a neomycin phosphotransferase II transgene correlates with the accumulation of unproductive RNAs and with increased cytosine methylation of 3' flanking regions
110. Both sense and antisense RNAs are targets for the sense transgene-induce posttranscriptional silencing mechanism
111. Enkephalins produced in transgenic plants using modified 2S seed storage proteins
112. Identification of a general silencer for 19S and 35S promoters in a transgenic tobacco plant: 90 bp of homology in the promoter sequences are sufficient for trans-inactivation
113. Transgene-induced gene silencing in plants
114. Stable accumulation of Aspergillus niger phytase in transgenic tobacco leaves
115. Systemic signalling in gene silencing
116. Reduced virus infectivity in N. tabacum secreting a TMV-specific full-size antibody
117. High frequency, heat treatment-induced inactivation of the phosphinotricin resistance gene in transgenic Single Cell suspension cultures of Medicago Sativa
118. RNA-directed de novo methylation of genomic sequences in plants
119. Virus resistance and gene silencing: Killing the messenger
120. Frequent collinear long transfer of DNA inclusive of the whole binary vector during Agrobacterium-mediated transformation
121. Commercial production of β-glucuronidase (GUS): A model system for the production of proteins in plants
122. Transcription control: Repressed repeats express themselves
123. Fluorescence in situ hybridisation on extended DNA fibers as a tool to analyze complex T-DNA loci in potato
124. Cytosine methylation and the ecology of intragenomic parasites
125. A humanized monoclonal antibody produced in transgenic plants for immunoprotection of the vagina against genital herpes
126. Commercial production of aprotinin in transgenic maize seeds