Template/primer requirements and single nucleotide incorporation by Hepatitis C virus nonstructural protein 5B polymerase

Journal of Virology

Volumn 74, Issue 19, 2000, Pages 9134-9143

  Zhong, W ^a   Ferrari, E ^a   Lesburg, C A ^a   Maag, D ^a   Ghosh, S K B ^a   Cameron, C E ^a   Lau, J Y N ^a   Hong, Z ^a  

^a Valeant Pharmaceuticals International   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DINUCLEOTIDE; NONSTRUCTURAL PROTEIN 5B POLYMERASE; PRIMER RNA; RNA DIRECTED RNA POLYMERASE; TRINUCLEOTIDE; UNCLASSIFIED DRUG; VIRUS ENZYME;

ARTICLE; BASE PAIRING; CATALYSIS; CONTROLLED STUDY; CRYSTAL STRUCTURE; ENZYME ACTIVE SITE; ENZYME ACTIVITY; HEPATITIS C VIRUS; NONHUMAN; PRIORITY JOURNAL; PROTEIN RNA BINDING; REACTION ANALYSIS; RNA CONFORMATION; RNA REPLICATION;

GENOME, VIRAL; HEPACIVIRUS; HUMAN; RNA REPLICASE; SUPPORT, U.S. GOV'T, P.H.S.; TEMPLATES, GENETIC; VIRAL NONSTRUCTURAL PROTEINS; VIRUS REPLICATION;

EID: 0033803374     PISSN: 0022538X     EISSN: None     Source Type: Journal    
DOI: 10.1128/JVI.74.19.9134-9143.2000     Document Type: Article

References (38)

1. Crystal structure of the RNA-dependent RNA polymerase of hepatitis C virus
2. Expression of recombinant hepatitis C virus NS5B
3. A thermodynamic study of unusually stable RNA and DNA hairpins
4. Poliovirus RNA-dependent RNA polymerase (3D(pol)): Assembly of stable, elongation-competent complexes by using a symmetrical primer/template substrate (sym/sub)
5. Identification and properties of the RNA-dependent RNA polymerase of hepatitis C virus
6. Crystal structure of the RNA-dependent RNA polymerase of hepatitis C virus
7. Structure of a transcribing T7 RNA polymerase initiation complex
8. Isolation of a cDNA clone derived from a blood-borne non-A, non-B viral hepatitis genome
9. RNA-dependent RNA polymerase of hepatitis C virus
10. Characterization of soluble hepatitis C virus RNA-dependent RNA polymerase expressed in Escherichia coli
11. Structure of a covalently trapped catalytic complex of HIV-1 reverse transcriptase: Implications for drug resistance
12. Expression of hepatitis C virus NS5B protein: Characterization of its RNA polymerase activity and RNA binding
13. Kinetic mechanism of transcription initiation by bacteriophage T7 RNA polymerase
14. Identification of a highly conserved sequence element at the 3! terminus of hepatitis C virus genome RNA
15. MOLSCRIPT: A program to produce both detailed and schematic plots of protein structure
16. Mutational analysis of bovine viral diarrhea virus RNA-dependent RNA polymerase
17. Crystal structure of the RNA-dependent RNA polymerase from hepatitis C virus reveals a fully encircled active site
18. Biochemical properties of hepatitis C virus NS5B RNA-dependent RNA polymerase and identification of amino acid sequence motifs essential for enzymatic activity
19. Replication of subgenomic hepatitis C virus RNAs in a hepatoma cell line
20. Biochemical and kinetic analyses of NS5B RNA-dependent RNA polymerase of the hepatitis C virus
21. De novo initiation of RNA synthesis by the RNA-dependent RNA polymerase (NS5B) of hepatitis C virus
22. Evolutionary role of abortive transcript as a primer for DNA replication
23. Raster3D: Photorealistic molecular graphics
24. Hepatitis C virus shares amino acid sequence similarity with pestiviruses and flaviruses as well as members of two plant virus supergroups
25. Use of ultra stable UNCG tetraloop hairpins to fold RNA structures: Thermodynamic and spectroscopic applications
26. Abortive products as initiating nucleotides during transcription by T7 RNA polymerase
27. Protein folding and association: Insights from interfacial and thermodynamic properties of hydrocarbons
28. A recombinant hepatitis C virus RNA-dependent RNA polymerase capable of copying the full-length viral RNA
29. Purification and properties of poliovirus RNA polymerase expressed in Escherichia coli
30. Flaviviridae: The viruses and their replication
31. Hepatitis C virus infection is associated with the development of hepatocellular carcinoma
32. Hepatitis C virus as a causative agent of hepatocellular carcinoma
33. De novo RNA synthesis catalyzed by HCV RNA-dependent RNA polymerase
34. Structure of the 3' terminus of the hepatitis C virus genome
35. Biochemical characterization of a hepatitis C virus RNA-dependent RNA polymerase mutant lacking the C-terminal hydrophobic sequence
36. Hepatitis C. Seroprevalence of hepatitis C virus (HCV) in a population sample
37. RNA-dependent RNA polymemse activity of the soluble recombinant hepatitis C virus NS5B protein truncated at the C-terminal region
38. De novo initiation of RNA synthesis by hepatitis C virus nonstructural protein 5B polymerase