Biochemical characterization of a hepatitis C virus RNA-dependent RNA polymerase mutant lacking the C-terminal hydrophobic sequence

Journal of General Virology

Volumn 81, Issue 3, 2000, Pages 759-767

  Tomei, Licia ^a   Vitale, Rosa Letizia ^a   Incitti, Ilario ^a   Serafini, Sergio ^a   Altamura, Sergio ^a   Vitelli, Alessandra ^a   De Francesco, Raffaele ^a  

^a IRBM   (Italy)

Author keywords

[No Author keywords available]

Indexed keywords

MUTANT PROTEIN; NUCLEOTIDE; RNA POLYMERASE;

ARTICLE; BACULOVIRUS; BIOCHEMISTRY; CARBOXY TERMINAL SEQUENCE; CONTROLLED STUDY; ENZYME ACTIVITY; ESCHERICHIA COLI; HEPATITIS C VIRUS; INSECT; NONHUMAN; POLYMERIZATION; PRIORITY JOURNAL; PROTEIN PURIFICATION; SOLUBILITY; VIRUS RECOMBINANT;

ESCHERICHIA COLI; HEPATITIS C VIRUS; INSECTA; RNA VIRUSES; UNIDENTIFIED BACULOVIRUS;

EID: 0033956270     PISSN: 00221317     EISSN: None     Source Type: Journal    
DOI: 10.1099/0022-1317-81-3-759     Document Type: Article

References (24)

1. Alter, H. J. (1995). To C or not to C: these are the questions. Blood 85, 1681-1695.
2. Behrens, S.-E., Tomei, L. & De Francesco, R. (1996). Identification and properties of the RNA-dependent RNA polymerase of hepatitis C virus. EMBO Journal 15, 12-22.
3. Chien, D. Y., Choo, Q. L., Tabrizi, A., Kuo, C., McFarland, J., Berger, K., Lee, C., Shuster, J. R., Nguyen, T., Moyer, D. L., Tong, M. M., Furuta, S., Omata, M., Tegtmeyer, G., Alter, H., Schiff, E., Jeffers, L., Houghton, M. & Kuo, G. (1992). Diagnosis of hepatitis C virus (HCV) infection using an immunodominant chimeric polyprotein to capture circulating antibodies: reevaluation of the role of HCV in liver disease. Proceedings of the National Academy of Sciences, USA 89, 10011-10015.
4. Choo, Q. L., Kuo, G., Weiner, A. J., Overby, L. R., Bradley, D. W. & Houghton, M. (1989). Isolation of a cDNA clone derived from a blood-borne non-A, non-B viral hepatitis genome. Science 244, 359-362.
5. De Francesco, R., Behrens, S.-E., Tomei, L., Altamura, S. & Jiricny, J. (1996). RNA-dependent RNA polymerase of hepatitis C virus. Methods in Enzymology 275, 58-67.
6. De Francesco, R., Pessi, A. & Steinkühler, C. (1998). The hepatitis C virus NS3 proteinase: structure and function of a zinc-containing serine proteinase. In Therapies for Viral Hepatitis, pp. 235-245. Edited by R. F. Schinazi, J.-P. Sommadossi & H. C. Thomas. London: International Medical Press.
7. Ferrari, E., Wright-Minogue, J., Fang, J. W. S., Baroudy, B. M., Lau, J. Y. N. & Hong, Z. (1999). Characterization of soluble hepatitis C virus RNA-dependent RNA polymerase expressed in Escherichia coli. Journal of Virology 73, 1649-1654.
8. Hwang, S. B., Park, K. J., Kim, Y. S., Sung, Y. C. & Lai, M. M. C. (1997). Hepatitis C virus NS5B protein is a membrane-associated phosphoprotein with a predominantly perinuclear localization. Virology 227, 439-446.
9. Kuo, G., Choo, Q. L., Alter, H. J., Gitnick, G. L., Redeker, A. G., Purcell, R. H., Miyamura, T., Dienstag, J. L., Alter, M. J., Stevens, C. E., Tegtmeyer, G., Bonino, F., Colombo, M., Lee, W. S., Kuo, C., Berger, K., Shuster, J. R., Overby, L. R., Bradley, D. W. & Houghton, M. (1989). An assay for circulating antibodies to a major etiologic virus of human non-A, non-B hepatitis. Science 244, 362-364.
10. Kwong, A. D., Kim, J. L., Rao, G., Lipovsek, D. & Raybuck, S. A. (1998). Hepatitis C virus NS3/4A protease. Antiviral Research 40, 1-18.
11. Lai, M. M. C. (1998). Cellular factors in the transcription and replication of viral RNA genomes: a parallel to DNA-dependent RNA transcription. Virology 244, 1-12.
12. Lohmann, V., Korner, F., Herian, U. & Bartenschlager, R. (1997). Biochemical properties of hepatitis C virus NS5B RNA-dependent RNA polymerase and identification of amino acid sequence motifs essential for enzymatic activity. Journal of Virology 71, 8416-8428.
13. Lohmann, V., Roos, A., Korner, F., Koch, J. O. & Bartenschlager, R. (1998). Biochemical and kinetic analyses of NS5B RNA-dependent RNA polymerase of the hepatitis C virus. Virology 249, 108-118.
14. Pata, J. D., Schultz, S. C. & Kirkegaard, K. (1995). Functional oligomerization of poliovirus RNA-dependent RNA polymerase. RNA 1, 466-477.
15. Paul, A. V., van Boom, J. H., Filippov, D. & Wimmer, E. (1998). Protein-primed RNA synthesis by purified poliovirus RNA polymerase. Nature 393, 280-284.
16. pol. Journal of Virology 69, 7169-7179.
17. Richards, O. C. & Ehrenfeld, E. (1998). Effects of poliovirus 3AB protein on 3D polymerase-catalyzed reaction. Journal of Biological Chemistry 273, 12832-12840.
18. Salas, M. (1991). Protein-priming of DNA replication. Annual Review of Biochemistry 60, 39-71.
19. Studier, F. W., Rosenberg, A. H., Dunn, J. J. & Dubendorff, J. W. (1990). Use of T7 RNA polymerase to direct expression of cloned genes. Methods in Enzymology 185, 60-89.
20. Tanji, Y., Hijikata, M., Satoh, S., Kaneko, T. & Shimotohno, K. (1995). Hepatitis C virus-encoded nonstructural protein NS4A has versatile functions in viral protein processing. Journal of Virology 69, 1575-1581.
21. Towner, J. S., Ho, T. V. & Semler, B. L. (1996). Determinants of membrane association for poliovirus prolein 3AB. Journal of Biological Chemistry 271, 26810-26818.
22. Wilson, J. E., Porter, D. J. T. & Reardon, J. E. (1996). Inhibition of viral polymerases by chain-terminating substrates: a kinetic analysis. Methods in Enzymology 275, 398-424.
23. Yamashita, T., Kaneko, S., Shirota, Y., Qin, W., Nomura, T., Kobayashi, K. & Murakami, S. (1998). RNA-dependent RNA polymerase activity of the soluble recombinant hepatitis C virus NS5B protein truncated at the C-terminal region. Journal of Biological Chemistry 273, 15479-15486.
24. Yuan, Z. H., Kumar, U., Thomas, H. C., Wen, Y.-M. & Monjardino, J. (1997). Expression, purification, and partial characterization of HCV RNA polymerase. Biochemical and Biophysical Research Communications 232, 231-235.