Recent advances in the analysis of HCV NS5B RNA-dependent RNA polymerase

Current Opinion in Investigational Drugs

Volumn 1, Issue 3, 2000, Pages 289-296

  Lesburg, C A ^a   Radfar, R ^a   Weber, P C ^a  

^a MERCK RESEARCH LABORATORIES   (United States)

Author keywords

Crystal structure; Hepatitis C virus; NS5B protein; RNA dependent RNA polymerase; Structure assisted drug design; Viral replication

Indexed keywords

ANTIVIRUS AGENT; BARBITURIC ACID DERIVATIVE; BENZOTHIOPHENE DERIVATIVE; BUTYRIC ACID DERIVATIVE; GREEN FLUORESCENT PROTEIN; INDOLE DERIVATIVE; NUCLEOSIDE ANALOG; NUCLEOSIDE DERIVATIVE; NUCLEOTIDYLTRANSFERASE INHIBITOR; OLIGONUCLEOTIDE; RHODANINE; RNA DIRECTED RNA POLYMERASE; RNA DIRECTED RNA POLYMERASE INHIBITOR; UNCLASSIFIED DRUG; VIRUS PROTEIN; VP 32947;

AMINO ACID SEQUENCE; ANTIVIRAL ACTIVITY; CRYSTAL STRUCTURE; DNA TEMPLATE; DRUG DESIGN; ENZYME ACTIVITY; ENZYME PURIFICATION; EXPERIMENTAL MODEL; FLAVIVIRUS; HEPATITIS C; HEPATITIS C VIRUS; HUMAN; NONHUMAN; NUCLEOTIDE SEQUENCE; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN POLYMERIZATION; REVIEW; SOLUBILIZATION; VIRUS INHIBITION; VIRUS REPLICATION;

AMINO ACID SEQUENCE; ENZYME INHIBITORS; HEPACIVIRUS; HUMANS; MOLECULAR SEQUENCE DATA; RNA REPLICASE;

EID: 0033692476     PISSN: 09678298     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Review

References (42)

1. Flaviviridae: The viruses and their replication
2. Molecular virology of the hepatitis C virus
3. Biochemical and immunologic properties of the non-structural proteins of the hepatitis C virus: Implications for development of antiviral agents and vaccines
4. Biochemical and structural analysis of the NS5B RNA-dependent RNA polymerase of the hepatitis C virus
5. Characteristics of nucleotide substitution in the hepatitis C virus genome: Constraints on sequence change in coding regions at both ends of the genome
6. Hepatitis C virus genotypes and quasispecies
7. A recombinant hepatitis C virus RNA-dependent RNA polymerase capable of copying the full-length viral RNA
8. Specificity and mechanism analysis of hepatitis C virus RNA-dependent RNA polymerase
9. Characterization of soluble hepatitis C virus RNA-dependent RNA polymerase expressed in Escherichia coli
10. De novo initiation of RNA synthesis by the RNA-dependent RNA polymerase (NS5B) of hepatitis C virus
11. Biochemical characterization of a hepatitis C virus RNA-dependent RNA polymerase mutant lacking the C-terminal hydrophobic sequence
12. RNA-dependent RNA polymerase activity of the soluble recombinant hepatitis C virus NS5B protein truncated at the C-terminal region
13. Hepatitis C virus NS5B RNA replicase specifically binds ribosomes
14. Identification of four conserved motifs among the RNA-dependent polymerase encoding elements
15. Primary structural comparison of RNA-dependent polymerases from plant, animal and bacterial viruses
16. Template requirement and initiation site selection by hepatitis C virus polymerase on a minimal viral RNA template
17. De novo RNA synthesis catalyzed by HCV RNA-dependent RNA polymerase
18. De novo initiation of RNA synthesis by hepatitis C virus non-structural protein 5B polymerase
19. Template/primer requirements and single nucleotide incorporation by hepatitis C virus non-structural protein 5B polymerase
20. Biochemical and kinetic analyses of NS5B RNA-dependent RNA polymerase of the hepatitis C virus
21. The RNA-dependent RNA polymerases of different members of the family Flaviviridae exhibit similar properties in vitro
22. Selective stimulation of hepatitis C virus and pestivirus NS5B RNA polymerase activity by GTP
23. Expression of hepatitis C virus NS5B protein: Characterization of its RNA polymerase activity and RNA binding
24. Identification of terminal adenylyl transferase activity of the poliovirus polymerase 3Dpol
25. Biochemical properties of hepatitis C virus NS5B RNA-dependent RNA polymerase and identification of amino acid sequence motifs essential for enzymatic activity
26. Only a small fraction of purified hepatitis C RNA-dependent RNA polymerase is catalytically competent: Implications for viral replication and in vitro assays
27. Crystal structure of the RNA-dependent RNA polymerase of hepatitis C virus
28. Crystal structure of the RNA-dependent RNA polymerase of hepatitis C virus
29. Crystal structure of the RNA-dependent RNA polymerase from hepatitis C virus reveals a fully encircled active site
30. Polymerase structures and function: Variations on a theme?
31. Conformational coupling in DNA polymerase fidelity
32. Structure of a covalently trapped catalytic complex of HIV-1 reverse transcriptase: Implications for drug resistance
33. Structure of the RNA-dependent RNA polymerase of poliovirus
34. Structural and functional insights provided by crystal structures of DNA polymerases and their substrate complexes
35. Crystal structure of human immunodeficiency virus type 1 reverse transcriptase complexed with double-stranded DNA at 3.0 Å resolution shows bent DNA
36. Molecular views of viral polyprotein processing revealed by the crystal structure of the hepatitis C virus bifunctional protease-helicase
37. Complex formation of NS5B with NS3 and NS4A proteins of hepatitis C virus
38. Hepatitis C virus RNA polymerase and NS5A complex with a SNARE-like protein
39. Candidate targets for hepatitis C virus-specific antiviral therapy
40. Mechanism of action of a pestivirus antiviral compound
41. Hepatitis C virus-encoded enzymatic activities and conserved RNA elements in the 3' non-translated region are essential for virus replication in vivo
42. Replication of subgenomic hepatitis C virus RNAs in a hepatoma cell line