Unnatural amino acids as probes of protein structure and function

Current Opinion in Chemical Biology

Volumn 4, Issue 6, 2000, Pages 645-652

  Dougherty, Dennis A ^a  

^a California Institute of Technology   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACID; ION CHANNEL; PROTEIN; RNA;

ACYLATION; ESCHERICHIA COLI; FLUORESCENCE; GENE EXPRESSION SYSTEM; HYDROGEN BOND; MUTATION; PROTEIN STRUCTURE; REVIEW; RNA TRANSLATION; SITE DIRECTED MUTAGENESIS; STOP CODON; STRUCTURE ACTIVITY RELATION; XENOPUS;

EID: 0033637431     PISSN: 13675931     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1367-5931(00)00148-4     Document Type: Review

References (45)

1. A new tool for studying protein structure and function
2. Probing protein structure and function with an expanded genetic code
3. Incorporation of noncoded amino acids by in vitro protein biosynthesis
4. Efficient incorporation of unsaturated methionine analogues into proteins in vivo
5. Quadruplet codons: Implications for code expansion and the specification of translation step size
6. Tetracycline-regulated suppression of amber codons in mammalian cells
7. T4 RNA ligase mediated preparation of novel 'chemically misacylated' tRNA(Phe)s
8. A general method for site-specific incorporation of unnatural amino acids into proteins
9. Biosynthetic site-specific incorporation of a non-natural amino acid into a polypepfide
10. Development of improved tRNAs for in vitro biosynthesis of proteins containing unnatural amino acids
11. Nicotinic receptor binding site probed with unnatural amino-acid incorporation in intact cells
12. An engineered Tetrahymena tRNA(Gln) for in vivo incorporation of unnatural amino acids into proteins by nonsense suppression
13. Heterologous expression of excitability proteins: Route to more specific drugs?
14. Site-specific incorporation of biotinylated amino acids as a means to identify surface exposed residues in integral membrane proteins
15. Nicotinic receptors at the amino acid level
16. Localization of agonist and competitive antagonist binding sites on nicotinic acetylcholine receptors
17. Determinants of nicotinic receptor gating in natural and unnatural side chain structures at the M2 9' position
18. Cation-π interactions in chemistry and biology. A new view of benzene, Phe, Tyr, and Trp
19. The cation-π interaction
20. From ab initio quantum mechanics to molecular neurobiology: A cation-π binding site in the nicotinic receptor
21. Energetic analysis of an engineered cation-π interaction in staphylococcal nuclease
22. A model for hydride transfer in thymidylate synthase based on unnatural amino acid mutagenesis
23. Analysis of the role of the active site tyrosine in human glutathione transferase A1-1 by unnatural amino acid mutagenesis
24. Site-specific incorporation of biophysical probes into proteins
25. In vitro site-specific incorporation of fluorescent probes into β-galactosidase
26. Facile characterization of translation initiation via nonsense codon suppression
27. Probing the structure and function of the tachykinin neurokinin-2 receptor through biosynthetic incorporation of fluorescent amino acids at specific sites
28. Probing the role of an active site aspartic acid in dihydrofolate reductase
29. Structurally modified firefly luciferase. Effects of amino acid substitution at position 286
30. Controlling cell chemistry with caged compounds
31. Photochemically initiated protein splicing
32. A photochemical switch for controlling protein-protein interactions
33. Flash decaging of tyrosine sidechains in an ion channel
34. Site-specific, photochemical proteolysis applied to ion channels in vivo
35. Incorporation of esters into proteins: Improved synthesis of hydroxyacyl tRNAs
36. An experimental approach to evaluating the role of backbone interactions in proteins using unnatural amino acid mutagenesis
37. Mutational analysis of backbone hydrogen bonds in staphylococcal nuclease
38. Acetycholine receptor channel imaged in the open state
39. The location of the gate in the acetylcholine receptor channel
40. Backbone mutations in transmembrane domains of a ligand-gated ion channel: Implications for the mechanism of gating
41. 3 receptor gating
42. Mapping disulfide connectivity using backbone ester hydrolysis
43. Families of ion channels with two hydrophobic segments
44. Effects of release factor 1 on in vitro protein translation and the elaboration of proteins containing unnatural amino acids
45. Progress toward the evolution of an organism with an expanded genetic code