Optimization of retroviral gene transduction of mobilized primitive hematopoietic progenitors by using thrombopoietin, Flt3, and Kit ligands and RetroNectin culture

Human Gene Therapy

Volumn 10, Issue 11, 1999, Pages 1743-1752

  Murray, Lesley ^a   Luens, Karin ^a   Tushinski, Robert ^a   Jin, Liang ^a   Burton, Michelle ^a   Chen, Jingyi ^a   Forestell, Sean ^a   Hill, Beth ^a  

^a NOVARTIS PHARMA AG   (Switzerland)

Author keywords

[No Author keywords available]

Indexed keywords

FIBRONECTIN; FLT3 LIGAND; INTERLEUKIN 3; INTERLEUKIN 6; LEUKEMIA INHIBITORY FACTOR; NERVE GROWTH FACTOR RECEPTOR; PEPTIDE FRAGMENT; RETRONECTIN; STEM CELL FACTOR; THROMBOPOIETIN; UNCLASSIFIED DRUG; VIRUS VECTOR;

ARTICLE; CELL COUNT; CELL CULTURE; CELL VIABILITY; CENTRIFUGATION; COCULTURE; GENE EXPRESSION; GENE TARGETING; GENETIC TRANSDUCTION; HEMATOPOIETIC STEM CELL; HUMAN; HUMAN CELL; REPORTER GENE; RETROVIRUS; STROMA CELL; TRANSGENE;

ANTIGENS, CD34; CELLS, CULTURED; CYTOKINES; FIBRONECTINS; FLOW CYTOMETRY; GENE THERAPY; GENETIC VECTORS; HEMATOPOIETIC STEM CELL MOBILIZATION; HEMATOPOIETIC STEM CELLS; HUMANS; MEMBRANE PROTEINS; NERVE GROWTH FACTOR; RETROVIRIDAE; STEM CELL FACTOR; STROMAL CELLS; THROMBOPOIETIN; TRANSDUCTION, GENETIC;

UNIDENTIFIED RETROVIRUS;

EID: 0033587581     PISSN: 10430342     EISSN: None     Source Type: Journal    
DOI: 10.1089/10430349950017428     Document Type: Article

References (36)

1. AGARWAL, M., AUSTIN, T.W., MOREL, F., CHEN, J., BOHNLEIN, E., and PLAVEC, I. (1998). Scaffold attachment region-mediated enhancement of retroviral vector expression in primary T cells. J. Virol. 72, 3720-3728.
2. AMADO, R.G., SYMONDS, G., JAMIESON, B.D., ZHAO, G., ROSENBLATT, J.D., and ZACK, J.A. (1998). Effects of megakaryocyte growth and development factor on survival and retroviral transduction of T lymphoid progenitor cells. Hum. Gene Ther. 9, 173-183.
3. BAHNSON, A.B., DUNIGAN, J.T., BAYSAL, B.E., MOHNEY, T., ATCHISON, R.W., NIMGAONKAR, M.T., BALL, E.D., and BARRANGER, J.A. (1995). Centrifugal enhancement of retroviral-mediated gene transfer. J. Virol. Methods 54, 131-143.
4. CHENG, L., DU, C., LAVAU, C., CHEN, S., TONG, J., CHEN, B.P., SCOLLAY, R., HAWLEY, R.G., and HILL, B. (1998). Sustained gene expression in retrovirally transduced, engrafting human hematopoietic stem cells and their lympho-myeloid progeny. Blood 92, 1-11.
5. CONNEALLY, E., BARDY, P., EAVES, C.J., THOMAS, T., CHAPPEL, S., SHPALL, E.J., and HUMPHRIES, R.K. (1996). Rapid and efficient selection of human haematopoietic cells expressing murine heat-stable antigen as an indicator of retroviral-mediated gene transfer. Blood 87, 456-464.
6. + progenitors to sustain long-term hematopoiesis in immune-deficient mice after ex vivo retroviral-mediated transduction. Blood 89, 446-456.
7. DAO, M.A., HASHINO, K., KATO, I., and NOLTA, J.A. (1998). Adhesion to fibronectin maintains regenerative capacity during ex vivo culture and transduction of human hematopoietic stem and progenitor cells. Blood 92, 4612-4621.
8. DUNBAR, C.E., COTTLER-FOX, M., O'SHAUGHNESSY, J.A., DOREN, S., CARTER, C., BERENSON, R., BROWN, S., MOEN, R.C., GREENBLATT, J., STEWART, F.M., LEITMAN, S.F., WILSON, W.H., COWAN, K., YOUNG, N.S., and NIENHUIS, A.W. (1995). Retrovirally marked CD34-enriched peripheral blood and bone marrow cells contribute to long-term engraftment after autologous transplantation. Blood 85, 3048-3057.
9. + cells from patients with Gaucher disease: In vivo detection of transduced cells without myeloablation. Hum. Gene Ther. 9, 2629-2640.
10. FORESTELL, S.P., DANDO, J.S., CHEN, J., DE VRIES, P., BOHNLEIN, E., and RIGG, R.J. (1997). Novel retroviral packaging cell lines: Complementary tropisms and improved vector production for efficient gene transfer. Gene Ther. 4, 600-610.
11. lo human bone marrow cells reselected after long term exposure to GALV-pseudotyped retroviral vector. Hum. Gene Ther. 8, 2079-2086.
12. + hematopoietic cells. Hum. Gene Ther. 8, 2193-2206.
13. HAYLOCK, D.N., HORSFALL, J., DOWSE, T.L., RAMSHAW, H.S., NIUTTA, S., PROTOPSALTIS, S., PENG, L., BURRELL, C., RAPPOLD, I., BUHRING, H.-J., and SIMMONS, P.J. (1997). Increased recruitment of hematopoietic progenitor cells underlies the ex vivo expansion potential of flt3 ligand. Blood 90, 2260-2272.
14. JACOBSEN, S.E.W., BORGE, O.J., RAMSFJELL, V., CUI, L., CARDIER, J.E., VEIBY, O.P., MURPHY, M.J., and LOK, S. (1996). Thrombopoietin, a direct stimulator of viability and multilineage growth of primitive bone marrow progenitor cells. Stem Cells 14(Suppl. 1), 173-180.
15. KIEM, H.-P., ANDREWS, R.G., MORRIS, J., PETERSON, L., HEYWARD, S., ALLEN, J.M., RASKO, J.E.J., POTTER, J., and MILLER, A.D. (1998). Improved gene transfer into baboon marrow repopulating cells using recombinant human fibronectin fragment CH-296 in combination with interleukin-6, stem cell factor, flt-3 ligand, and megakaryocyte growth and development factor. Blood 92, 1878-1886.
16. + human blood cells. Stem Cells 15, 347-352.
17. KOHN, D.B., WEINBERG, K.I., NOLTA, J.A., HEISS, L.N., LENARSKY, C., CROOKS, G.M., HANLEY, M.E., ANNETT, G., BROOKS, J.S., EL-KHOUREIY, A., LAWRENCE, K., WELLS, S., MOEN, R.C., BASTIAN, J., WILLIAMS-HERMAN, D.E., ELDER, M., WARA, D., BOWEN, T., HERSHFIELD, M.S., MULLEN, C.A., BLAESE, R.M., and PARKAM, R. (1995). Engraftment of gene-modified umbilical cord blood cells in neonates with adenosine deaminase deficiency. Nature Med. 1, 1017-1023.
18. KU, H., YONEMURA, Y., KAUSHANSKY, K., and OGAWA, M. (1996). Thrombopoietin, the ligand for the Mpl receptor, synergizes with steel factor and other early acting cytokines in supporting proliferation and differentiation of primitive hematopoietic progenitors of mice. Blood 87, 4544-4551.
19. + cells to protect them against alkylating agents. Hum. Gene Ther. 8, 1901-1910.
20. LU, M., ZHANG, N., MARUYAMA, M., HAWLEY, R.G., and HO, A.D. (1996). Retrovirus-mediated gene expression in hematopoietic cells correlates inversely with growth factor stimulation. Hum. Gene Ther. 7, 2263-2271.
21. - cells with preserved ability to engraft in SCID-hu bone. Blood 91, 1206-1215.
22. LUSKEY, B.D., ROSENBLATT, M., ZSEBO, K., and WILLIAMS, D.A. (1992). Stem cell factor, interleukin-3, and interleukin-6 promote retroviral mediated gene transfer into murine hematopoietic stem cells. Blood 80, 396-402.
23. low primitive cells capable of reconstituting long-term cultures in vitro and nonobese diabetic-severe combined immunodeficiency mice in vivo. Hum. Gene Ther. 9, 1497-1511.
24. McCOWAGE, G.B., PHILLIPS, K.L., GENTRY, T.L., HULL, S., KURTZBERG, J., GILBOA, E., and SMITH, C. (1998). Multiparameter-fluorescence activated cell sorting analysis of retroviral vector gene transfer into primitive umbilical cord blood cells. Exp. Hematol. 26, 288-298.
25. MILLER, D.G., ADAM, M.A., and MILLER, D. (1990). Gene transfer by retrovirus vectors occurs only in cells that are replicating at the time of infection. Mol. Cell Biol. 10, 4239-4242.
26. MORITZ, T., PATEL, V.P., and WILLIAMS, D.A. (1994). Bone marrow extracellular matrix molecules, improve gene transfer into human hematopoietic stem cells via retroviral vectors. J. Clin. Invest. 93, 1451-1457.
27. + cell subsets into peripheral blood and expands multilineage progenitors in bone marrow of cancer patients with normal hematopoiesis. Exp. Hematol. 26, 207-216.
28. + cells into rapid division. Exp. Hematol. 27, 1019-1028.
29. NOLTA, J.A., SMOGORZEWSKA, E.M., and KOHN, D.B. (1995). Analysis of optimal conditions for retroviral-mediated transduction of primitive human hematopoietic cells. Blood 86, 101-110.
30. +-restricted phenotypes of human hematopoietic progenitor cells by retrovirus-mediated gene transfer. Hum. Gene Ther. 8, 1611-1623.
31. SIEGERT, W., and SERKE, S. (1996). Cytokine mobilized blood CD34-expressing cells are not cycling. Bone Marrow Transplant 17, 467-470.
32. TUSHINSKI, R., DE VRIES, P., MOON, J., POLIKOF, D., BOEHNLEIN, E., and TSUKAMOTO, A. (1997). The effect of SCF, LIF or Flt3L in combination with IL3 and IL6 on the retroviral gene transduction of hematopoietic stem cells. Exp. Hematol. 25, 890. [Abstract 566]
33. TUSHINSKI, R., JIN, L., FORESTELL, S., READING, C., and RIGG, R. (1998). The effect of flt3L, TPO and/or LIF in combination with IL3 and IL6 on ProPak-based retroviral gene transduction of hematopoietic stem cells. Exp. Hematol. 26, 760. [Abstract 275]
34. UCHIDA, N., HE, D., FRIERA, A., REITSMA, M., SASAKI, D., CHEN, B., and TSUKAMOTO, A. (1997). The unexpected G0/G1 cell cycle status of mobilized hematopoietic stem cells from peripheral blood. Blood 89, 465-472.
35. - primitive progenitor cells. Blood 88, 1619-1631.
36. + subset following cell division ex vivo. Exp. Hematol. 27, 994-1003.