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Rutledge-Cropsey K, Klungness JH, Abubakr SM: Performance of enzymically deinked recovered paper on paper machine runability. TAPPI J 1998, 81:148-151. Compared to chemical deinking processes, pulp deinked with cellulases results in improved pulp drainage, without impairing paper quality and energy conservation.
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Hsieh Y-L, Cram LA: Enzymatic hydrolysis to improve wetting and absorbency of polyester fabrics. Textile Res J 1998, 68:311-319. The properties of polyester fabrics can be improved by enzyme treatments that are more environmentally friendly and energy conserving than corresponding chemical processing.
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Yano T, Oue O, Kagamiyama H: Directed evolution of an aspartate aminotransferase with new substrate specificities. Proc Natl Acad Sci USA 1998, 95:5511-5515. This paper is a very good example of the experimental approach that can be taken to generate novel catalytic activities, and reveals the complexity of attempting a purely rational approach to protein design.
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Proc Natl Acad Sci USA
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Pedersen H, Holder S, Sutherlin DP, Schwitter U, King DS, Schultz PG: A method for directed evolution and functional cloning of enzymes. Proc Natl Acad Sci USA 1998, 95:10523-10528. This is a notable example of how a good screen can be combined efficiently with the methods used for the directed evolution of proteins.
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Proc Natl Acad Sci USA
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Pedersen, H.1
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Hopfner K-P, Kopetzki E, Kreβe G-B, Bode W, Huber R: New enzyme lineages by subdomain shuffling. Proc Nat Acad Sci USA 1998, 95:9813-9818. The development of successful new methodologies for obtaining novel catalysts is demonstrated by this study in which a process termed domain-shuffling is introduced. Combination of the amino-terminal sub-domain of coagulation factor X with the carboxyl terminus of trypsin created novel catalytic activity. Information on the structural features of proteins representing a diverse range of biological functions is growing at a tremendous pace. The techniques outlined in this paper should provide a means for rapidly exploiting this knowledge.
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Proc Nat Acad Sci USA
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Hopfner, K.-P.1
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Malakauskas SM, Mayo SL: Design, structure and stability of a hyperthermophilic protein variant. Nat Struct Biol 1998, 5:470-475. Rational protein design using computer algorithms is a key goal and represents the power of combining the methodologies from computing, physics, chemistry and biology. Although it is early days for this approach to provide the success that is ultimately envisaged, this paper indicates that its role in protein design will be very important.
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Nat Struct Biol
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Malakauskas, S.M.1
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Van den Burg B, Vriend G, Veltman OR, Venema G, Eijsink GH: Engineering an enzyme to resist boiling. Proc Natl Acad Sci USA 1998, 95:2056-2060. Hyperthermostability has been engineered into a bacterial metalloprotease by inserting amino acid residues found at equivalent positions in a naturally occurring extremozyme.
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Proc Natl Acad Sci USA
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Van Den Burg, B.1
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J Biol Chem
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Yip KSP, Britton KL, Stillman TJ, Lebbink J, DeVos WM, Robb FT, Vetriani C, Maeder D, Rice DW: Insights into the molecular basis of thermal stability from the analysis of ion-pair networks. Eur J Biochem 1998, 255:336-346. This research [20•,21•] originates from the Krebs Institute of Biomolecular Studies in Sheffield and illustrates the type of fundamental work that must be done to underpin and extend the understanding of extremozyme function. Although the enzyme, glutamate dehydrogenase, is common to both studies, it is clear that different structures have evolved for it to adapt to two different environments.
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Eur J Biochem
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MacBeath G, Kast P, Hilvert D: Redesigning enzyme topology by directed evolution. Science 1998, 279:1958-1961.
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Zhao H, Giver L, Shao Z, Affholter JA, Arnold FH: Molecular evolution by staggered extension process (StEP) in vitro combination. Nat Biotechnol 1998, 16:258-261. This paper describes a useful new combinatorial method for the directed evolution of enzymes.
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Nat Biotechnol
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Zhao, H.1
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Partridge J, Halling PJ, Moore BD: Practical route to high activity enzyme preparations for synthesis in organic media. Chem Commun 1998, 841-842. Single-pot procedure for enabling high catalytic activities of proteases in organic media, the applicability of which needs to be tested with other enzymes.
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Chem Commun
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J Ferm Bioeng
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Dordick JS, Novick SJ, Sergeeva MV: Biocatalytic plastics. Chem Indy 1998, 5:17-20. A review of the preparation, properties and potential use of enzymes immobilised in plastic materials. The range of plastic structures that can be formulated and reused, and applications in fields such as chemical synthesis, downstream processing, agriculture, fouling prevention, and medicine, present dramatic possibilities for environmentally friendly developments.
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Chem Indy
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Noritomi H, Koyama K, Kato S, Nagahama K: Increased thermostability of cross-linked enzyme crystals of subtilisin in organic solvents. Biotechnol Tech 1998, 12:467-469.
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Aoun S, Baboulène M: Regioselective bromohydroxylation of alkenes catalyzed by chloroperoxidase: Advantages of the immobilization of enzyme on talc. J Mol Catal B: Enz 1998, 4:101-109.
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J Mol Catal B: Enz
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Enz Microb Technol
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Fernandez-Lafuente R, Rosell CM, Caanan-Haden L, Rodes L, Guisan JM: Facile synthesis of artificial enzyme nano-environments via solid-phase chemistry of immobilized derivatives: Dramatic stabilization of penicillin acylase versus organic solvents. Enz Microb Technol 1999, 24:96-103. Post-immobilisation modification with polyamine and polyaldehydes imparts remarkable stability to penicillin acylase and offers the possibility of catalyst reuse and opportunities for new process developments.
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Enz Microb Technol
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Millqvist-Fureby A, Gill IS, Vulfson EN: Enzymatic transformations in supersaturated solution: II. Synthesis of disaccharides via transglycosylation. Biotechnol Bioeng 1998, 60:197-203.
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Millqvist-Fureby A, Gao C, Vulfson EN: Regioselective synthesis of ethoxylated glycoside esters using β-glucosidase in supersaturated solutions and upases inorganic solvents. Biotechnol Bioeng 1998, 59:747-753.
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J Chem Technol Biotechnol
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Abstr Am Chem Soc
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Athès V, Combes D: Influence of additives on high pressure stability of β-galactosidase from Kluyveromyces lactis and invertase from Saccharomyces cerevisiae. Enz Microb Technol 1998, 22:532-537. This paper demonstrates the successful stabilisation of glycosidases for use in high pressure food processing.
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Enz Microb Technol
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Fernanedez-Lafuente R, Guisan JM, Pregnolato M, Terreni M: Chemoenzymatic one-pot synthesis of cefazolin from cephalosporin C in fully aqueous medium, involving three consecutive biotransformations catalyzed by D-aminoacid oxidase, glutaryl acylase and penicillin G acylase. Tetrahedron Lett 1997, 38:4693-4696. A good example of the potential utility of biotransformations in multistep enzyme reactions, and the environmental benefits deriving from such an approach.
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Tetrahedron Lett
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Michels PC, Khmelnitsky YL, Dordick JS, Clark DS: Combinatorial biocatalysis: A natural approach to drug discovery. TIBTECH 1998, 16:210-215. An excellent review of the emerging technology of combinatorial biocatalysis, with examples chosen by the leaders in the field. Draws a clear distinction between combinatorial biocatalysis and combinatorial biosynthesis.
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TIBTECH
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Regioselective enzymatic acylation as a tool for producing solution-phase combinatorial libraries
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Mozhaev VV, Budde CL, Rich JO, Dordick JS: Regioselective enzymatic acylation as a tool for producing solution-phase combinatorial libraries. Tetrahedron 1998, 54:3971-3982. Enzymes were used in a combinatorial approach for the generation of a library of an acylated flavonoid, bergenin. The regioselective functionality achieved with enzymes was not possible via chemical acylation methods, thus demonstrating the power of biocatalysts in synthesising multifunctional lead compounds for the production of novel chemicals.
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(1998)
Tetrahedron
, vol.54
, pp. 3971-3982
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Mozhaev, V.V.1
Budde, C.L.2
Rich, J.O.3
Dordick, J.S.4
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