-
1
-
-
0028110130
-
DNA shuffling by random fragmentation and reassembly: In vitro recombination for molecular evolution
-
Stemmer, W.P.C. DNA shuffling by random fragmentation and reassembly: in vitro recombination for molecular Evolution. Proc. Natl. Acad. Sci. USA 91, 10747-10751 (1994).
-
(1994)
Proc. Natl. Acad. Sci. USA
, vol.91
, pp. 10747-10751
-
-
Stemmer, W.P.C.1
-
2
-
-
0028050350
-
Rapid evolution of a protein in vitro by DNA shuffling
-
Stemmer, W.P.C. Rapid evolution of a protein in vitro by DNA shuffling. Nature 370, 389-391 (1994).
-
(1994)
Nature
, vol.370
, pp. 389-391
-
-
Stemmer, W.P.C.1
-
3
-
-
0030989062
-
Directed evolution of a fucosidase from a galactosidase by DNA shuffling and screening
-
Zhang, J.-H., Dawes, G. & Stemmer, W.P.C. Directed evolution of a fucosidase from a galactosidase by DNA shuffling and screening. Proc. Natl. Acad. Sci. USA 94, 4504-4509 (1997).
-
(1997)
Proc. Natl. Acad. Sci. USA
, vol.94
, pp. 4504-4509
-
-
Zhang, J.-H.1
Dawes, G.2
Stemmer, W.P.C.3
-
4
-
-
0029670330
-
Improved green fluorescent protein by molecular evolution using DNA shuffling
-
Crameri, A.. Whitehorn, E.A., Tate, E. & Stemmer, W.P.C. Improved green fluorescent protein by molecular evolution using DNA shuffling. Nat. Biotechnol. 14, 315-319 (1996).
-
(1996)
Nat. Biotechnol.
, vol.14
, pp. 315-319
-
-
Crameri, A.1
Whitehorn, E.A.2
Tate, E.3
Stemmer, W.P.C.4
-
5
-
-
0031874849
-
Improved properties of FLP recombinase evolved by cycling mutagenesis
-
Buchholz, F., Angrand, P.-O. & Stewart, A.F. Improved properties of FLP recombinase evolved by cycling mutagenesis. Nat. Biotechnol. 16, 657-662 (1998).
-
(1998)
Nat. Biotechnol.
, vol.16
, pp. 657-662
-
-
Buchholz, F.1
Angrand, P.-O.2
Stewart, A.F.3
-
6
-
-
0032518266
-
DNA shuffling of a family of genes from diverse species accelerates directed evolution
-
Crameri, A., Raillard, S.-A., Bermudez. E. & Stemmer, W.P.C. DNA shuffling of a family of genes from diverse species accelerates directed evolution. Nature 391, 288-291 (1998).
-
(1998)
Nature
, vol.391
, pp. 288-291
-
-
Crameri, A.1
Raillard, S.-A.2
Bermudez, E.3
Stemmer, W.P.C.4
-
7
-
-
0031874757
-
Enhanced degradation of polychlorinated biphenyls by directed evolution of biphenyl dioxygenase
-
Kumamaru, T., Suenaga, H., Mitsuoka, M., Watanabe, T. & Furukawa, K. Enhanced degradation of polychlorinated biphenyls by directed evolution of biphenyl dioxygenase. Nat. Biotechnol. 16, 663-666 (1998).
-
(1998)
Nat. Biotechnol.
, vol.16
, pp. 663-666
-
-
Kumamaru, T.1
Suenaga, H.2
Mitsuoka, M.3
Watanabe, T.4
Furukawa, K.5
-
8
-
-
0003084227
-
-
eds. Fields, B.N., Knipe, D.M. & Howley, P.M. Lippincott-Raven, Philadelphia
-
Hirsch, M.S., Kaplan, J.C. & D'Aquila, R.T. in Fields virology (eds. Fields, B.N., Knipe, D.M. & Howley, P.M.) 431-466 (Lippincott-Raven, Philadelphia, 1996).
-
(1996)
Fields Virology
, pp. 431-466
-
-
Hirsch, M.S.1
Kaplan, J.C.2
D'Aquila, R.T.3
-
10
-
-
0343052856
-
Use of herpes simplex virus thymidine kinase to improve the antiviral activity of zidovudine
-
Guettari, N., Loubiere, L., Brisson, E. & Klatzmann, D. Use of herpes simplex virus thymidine kinase to improve the antiviral activity of zidovudine. Virology 235, 398-405 (1997).
-
(1997)
Virology
, vol.235
, pp. 398-405
-
-
Guettari, N.1
Loubiere, L.2
Brisson, E.3
Klatzmann, D.4
-
11
-
-
0030795311
-
Metabolism and activities of 3′-azido-2′,3′-dideoxythymidine and 2′,3′-didehydro-2′,3′-dideoxythymidine in herpesvirus thymidine kinase transduced T-lymphocytes
-
Drake, R.R. et al. Metabolism and activities of 3′-azido-2′,3′-dideoxythymidine and 2′,3′-didehydro-2′,3′-dideoxythymidine in herpesvirus thymidine kinase transduced T-lymphocytes. Antiviral Res. 35, 177-185 (1997).
-
(1997)
Antiviral Res.
, vol.35
, pp. 177-185
-
-
Drake, R.R.1
-
12
-
-
0026464790
-
Viral thymidine kinases and their relatives
-
Gentry, G.A. Viral thymidine kinases and their relatives. Pharmacol. Ther. 54, 319-355 (1992).
-
(1992)
Pharmacol. Ther.
, vol.54
, pp. 319-355
-
-
Gentry, G.A.1
-
13
-
-
0027192014
-
Thymidine kinase mutants obtained by random sequence selection
-
Munir, K.M., French, D.C. & Loeb, L.A. Thymidine kinase mutants obtained by random sequence selection. Proc. Natl. Acad. Sci. USA 90, 4012-4016 (1993).
-
(1993)
Proc. Natl. Acad. Sci. USA
, vol.90
, pp. 4012-4016
-
-
Munir, K.M.1
French, D.C.2
Loeb, L.A.3
-
14
-
-
0027501570
-
Identification of important residues within the putative nucleoside binding site of HSV-1 thymidine kinase by random sequence selection: Analysis of selected mutants in vitro
-
Black, M.E. & Loeb, L.A. Identification of important residues within the putative nucleoside binding site of HSV-1 thymidine kinase by random sequence selection: analysis of selected mutants in vitro. Biochemistry 32, 11618-11626 (1993).
-
(1993)
Biochemistry
, vol.32
, pp. 11618-11626
-
-
Black, M.E.1
Loeb, L.A.2
-
15
-
-
0029883624
-
Creation of drug-specific herpes simplex virus type 1 thymidine kinase mutants for gene therapy
-
Black, M.E., Newcomb, T.G., Wilson, H.-M.P. & Loeb, L.A. Creation of drug-specific herpes simplex virus type 1 thymidine kinase mutants for gene therapy. Proc. Natl. Acad. Sci. USA 93, 3525-3529 (1996).
-
(1996)
Proc. Natl. Acad. Sci. USA
, vol.93
, pp. 3525-3529
-
-
Black, M.E.1
Newcomb, T.G.2
Wilson, H.-M.P.3
Loeb, L.A.4
-
16
-
-
0031587291
-
Strategies for the in vitro evolution of protein function: Enzyme evolution by random recombination of improved sequences
-
Moore, J.C., Jin, H.-M., Kuchner, O. & Arnold, F.H. Strategies for the in vitro evolution of protein function: enzyme evolution by random recombination of improved sequences. J. Mol. Biol. 273, 336-347 (1997).
-
(1997)
J. Mol. Biol.
, vol.273
, pp. 336-347
-
-
Moore, J.C.1
Jin, H.-M.2
Kuchner, O.3
Arnold, F.H.4
-
17
-
-
0030829025
-
The structures of thymidine kinase from Herpes simplex virus type 1 in complex with substrates and a substrate analog
-
Wild, K., Bohner, T., Folkers, G. & Schulz, G.E. The structures of thymidine kinase from Herpes simplex virus type 1 in complex with substrates and a substrate analog. Protein Sci. 6, 2097-2106 (1997).
-
(1997)
Protein Sci.
, vol.6
, pp. 2097-2106
-
-
Wild, K.1
Bohner, T.2
Folkers, G.3
Schulz, G.E.4
-
18
-
-
0029125949
-
Crystal structures of the thymidine kinase from herpes simplex virus type-1 in complex with deoxythymidine and ganciclovir
-
1995
-
Brown, D.G. et al. 1995. Crystal structures of the thymidine kinase from herpes simplex virus type-1 in complex with deoxythymidine and ganciclovir. Nat. Struct. Biol. 2, 876-881 (1995).
-
(1995)
Nat. Struct. Biol.
, vol.2
, pp. 876-881
-
-
Brown, D.G.1
-
19
-
-
0032529367
-
Exploring the active site of herpes simplex virus type-1 thymidine kinase by x-ray crystallography of complexes with acyclovir and other ligands
-
Champness, J.N. et al. Exploring the active site of herpes simplex virus type-1 thymidine kinase by x-ray crystallography of complexes with acyclovir and other ligands. Struct. Funct. Genet. 32, 350-361 (1998).
-
(1998)
Struct. Funct. Genet.
, vol.32
, pp. 350-361
-
-
Champness, J.N.1
-
20
-
-
0032528262
-
Drug resistance of herpes simplex virus type 1: Structural considerations on the molecular level of the thymidine kinase
-
Kussmann-Gerber, S., Kuonen, O., Folkers, G., Pilger, B.D. & Scapozza, L. Drug resistance of herpes simplex virus type 1: structural considerations on the molecular level of the thymidine kinase. Eur. J. Biochem. 255, 472-481 (1998).
-
(1998)
Eur. J. Biochem.
, vol.255
, pp. 472-481
-
-
Kussmann-Gerber, S.1
Kuonen, O.2
Folkers, G.3
Pilger, B.D.4
Scapozza, L.5
-
21
-
-
0030845913
-
Structure of thymidylate kinase reveals the cause behind the limiting step in AZT activation
-
Lavie, A. et al. Structure of thymidylate kinase reveals the cause behind the limiting step in AZT activation. Nat. Struct. Biol. 4, 601-604 (1997).
-
(1997)
Nat. Struct. Biol.
, vol.4
, pp. 601-604
-
-
Lavie, A.1
-
22
-
-
0030859182
-
The bottleneck in AZT activation
-
Lavie, A. et al. The bottleneck in AZT activation. Nat. Med. 3, 922-924 (1997).
-
(1997)
Nat. Med.
, vol.3
, pp. 922-924
-
-
Lavie, A.1
-
23
-
-
0031930544
-
Improving AZT efficacy
-
Balzarini, J. et al. Improving AZT efficacy. Nat. Med. 4, 132 (1998).
-
(1998)
Nat. Med.
, vol.4
, pp. 132
-
-
Balzarini, J.1
-
24
-
-
0031034761
-
Overexpression of DNA polymerase b sensitizes mammalian cells to 2′,3′-didexoycytidine and 3′-azido′-3′-deoxythymidine
-
Bouayadi, K. et al. Overexpression of DNA polymerase b sensitizes mammalian cells to 2′,3′-didexoycytidine and 3′-azido′-3′-deoxythymidine. Cancer Res. 57, 110-116 (1997).
-
(1997)
Cancer Res.
, vol.57
, pp. 110-116
-
-
Bouayadi, K.1
-
25
-
-
0030722164
-
Applications of DNA shuffling to pharmaceuticals and vaccines
-
Patten, P.A., Howard, R.J. & Stemmer, W.P.C. Applications of DNA shuffling to pharmaceuticals and vaccines. Curr. Opin. Biotechnol. 8, 724-733 (1997).
-
(1997)
Curr. Opin. Biotechnol.
, vol.8
, pp. 724-733
-
-
Patten, P.A.1
Howard, R.J.2
Stemmer, W.P.C.3
-
26
-
-
0014152202
-
A deoxythymidine kinase deficient mutant of Escherichia coli. II. Mapping and transduction studies with phage phi 80
-
Igarashi, K., Hiraga, S. & Yura, T. A deoxythymidine kinase deficient mutant of Escherichia coli. II. Mapping and transduction studies with phage phi 80. Genetics 57, 643-654 (1967).
-
(1967)
Genetics
, vol.57
, pp. 643-654
-
-
Igarashi, K.1
Hiraga, S.2
Yura, T.3
-
27
-
-
0018735516
-
Statistical analysis of enzyme data
-
Cleland, W.W. Statistical analysis of enzyme data. Methods Enzymol. 63, 103-138 (1979).
-
(1979)
Methods Enzymol.
, vol.63
, pp. 103-138
-
-
Cleland, W.W.1
-
28
-
-
0030741596
-
X-ray analysis of azido-thymidine diphosphate binding to nucleoside diphosphate kinase
-
Xu, Y. et al. X-ray analysis of azido-thymidine diphosphate binding to nucleoside diphosphate kinase. Proc. Natl. Acad. Sci. USA 94, 7162-7165 (1997).
-
(1997)
Proc. Natl. Acad. Sci. USA
, vol.94
, pp. 7162-7165
-
-
Xu, Y.1
-
29
-
-
0029633186
-
AMBER, A package of computer programs for applying molecular mechanics, normal mode analysis, molecular dynamics and free energy calculations to simulate the structural and energetic properties of molecules
-
Perlman, D.A. et al. AMBER, a package of computer programs for applying molecular mechanics, normal mode analysis, molecular dynamics and free energy calculations to simulate the structural and energetic properties of molecules. Comp. Phys. Commun. 91, 1-41 (1995).
-
(1995)
Comp. Phys. Commun.
, vol.91
, pp. 1-41
-
-
Perlman, D.A.1
-
30
-
-
0004016501
-
Comparison of simple potential functions for simulating liquid water
-
Jorgensen, W.L., Chrasekhar, J., Madura, J.D., Impey, R.W. & Klein, M. Comparison of simple potential functions for simulating liquid water. J. Chem. Phys. 79, 926-935 (1983).
-
(1983)
J. Chem. Phys.
, vol.79
, pp. 926-935
-
-
Jorgensen, W.L.1
Chrasekhar, J.2
Madura, J.D.3
Impey, R.W.4
Klein, M.5
-
31
-
-
0000243829
-
PROCHECK: A program to check the stereochemical quality of protein structures
-
Laskowski, R.A., MacArthur, M.W., Moos, D.S. & Thornton, J.M.J. PROCHECK: a program to check the stereochemical quality of protein structures. Appl. Cryst. 26, 283-291 (1993).
-
(1993)
Appl. Cryst.
, vol.26
, pp. 283-291
-
-
Laskowski, R.A.1
MacArthur, M.W.2
Moos, D.S.3
Thornton, J.M.J.4
-
32
-
-
0021871375
-
A computational procedure for determining energetically favorable binding sites on biologically important macromolecules
-
Goodford, P.J. A computational procedure for determining energetically favorable binding sites on biologically important macromolecules. J. Med. Chem. 28, 849-857 (1985).
-
(1985)
J. Med. Chem.
, vol.28
, pp. 849-857
-
-
Goodford, P.J.1
-
33
-
-
0027439587
-
Further development of hydrogen bond functions for use in determining energetically favorable binding sites on molecules of known structure
-
Wade, R.C. & Goodford, P.J. Further development of hydrogen bond functions for use in determining energetically favorable binding sites on molecules of known structure. J. Med. Chem. 36, 140-156 (1993).
-
(1993)
J. Med. Chem.
, vol.36
, pp. 140-156
-
-
Wade, R.C.1
Goodford, P.J.2
|