Directed evolution of thymidine kinase for AZT phosphorylation using DNA family shuffling

Nature Biotechnology

Volumn 17, Issue 3, 1999, Pages 259-264

  Christians, Fred C ^a,c   Scapozza, Leonardo ^b   Crameri, Andreas ^a   Folkers, Gerd ^b   Stemmer, Willem P C ^a  

^a MAXYGEN INC   (United States)

^b ETH ZURICH   (Switzerland)

^c Thermo Fisher Scientific   (United States)

Author keywords

Gene transfer; Random mutagenesis; Sexual PCR DNA shuffling; Suicide gene; Zidovudine

Indexed keywords

DNA; THYMIDINE KINASE; ZIDOVUDINE;

AMINO ACID SUBSTITUTION; ARTICLE; BINDING AFFINITY; BINDING SITE; CHIMERA; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME SPECIFICITY; ENZYME SUBSTRATE COMPLEX; GENE ISOLATION; GENETIC RECOMBINATION; MOLECULAR CLONING; PRIORITY JOURNAL; SEQUENCE HOMOLOGY;

AMINO ACID SEQUENCE; CELL DIVISION; CHIMERA; CLONING, MOLECULAR; COMPUTER SIMULATION; DOSE-RESPONSE RELATIONSHIP, DRUG; ESCHERICHIA COLI; HERPESVIRUS 1, HUMAN; HERPESVIRUS 2, HUMAN; HUMANS; HYDROGEN BONDING; KINETICS; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MUTAGENESIS; SEQUENCE HOMOLOGY, AMINO ACID; THYMIDINE KINASE; ZIDOVUDINE;

ANIMALIA; DNA VIRUSES; ESCHERICHIA; HERPES; HUMAN HERPESVIRUS 1; HUMAN HERPESVIRUS 2;

EID: 0033037012     PISSN: 10870156     EISSN: None     Source Type: Journal    
DOI: 10.1038/7003     Document Type: Article

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