X-ray crystal structures of 70S ribosome functional complexes

Science

Volumn 285, Issue 5436, 1999, Pages 2095-2104

  Cate, Jamie H ^a,c   Yusupov, Marat M ^a   Yusupova, Gulnara Zh ^a   Earnest, Thomas N ^b   Noller, Harry F ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b LAWRENCE BERKELEY NATIONAL LABORATORY   (United States)

^c WHITEHEAD INSTITUTE FOR BIOMEDICAL RESEARCH   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

MESSENGER RNA; TRANSFER RNA;

ANTICODON; ARTICLE; CHEMICAL INTERACTION; CHEMICAL STRUCTURE; CRYSTAL STRUCTURE; MOLECULAR DYNAMICS; NONHUMAN; PRIORITY JOURNAL; PROTEIN PROTEIN INTERACTION; RIBOSOME; STRUCTURE ANALYSIS; X RAY CRYSTALLOGRAPHY;

ANTICODON; BACTERIAL PROTEINS; BASE PAIRING; BINDING SITES; CRYSTALLIZATION; CRYSTALLOGRAPHY, X-RAY; FOURIER ANALYSIS; MODELS, MOLECULAR; NUCLEIC ACID CONFORMATION; PEPTIDE ELONGATION FACTORS; PROTEIN BIOSYNTHESIS; PROTEIN CONFORMATION; RIBOSOMAL PROTEINS; RIBOSOMES; RNA, BACTERIAL; RNA, MESSENGER; RNA, RIBOSOMAL; RNA, RIBOSOMAL, 16S; RNA, RIBOSOMAL, 23S; RNA, TRANSFER; THERMUS THERMOPHILUS;

EID: 0032881809     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.285.5436.2095     Document Type: Article

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128. 12; H. Taube for providing osmium (III) hexammine; D. Sussman, J. Nix, and J. Diener for help with data collection; L. Grate for programming; H. Bellamy of SSRL for help with preliminary synchrotron experiments; L.-W. Hung. G. McDermott, K. Henderon, and C. Cork for help at the ALS; J. Murray for help with hardware configuration; J. Doudna, F. Michel, and W. Scott for helpful comments on the manuscript; and W. Scott and A. and H. Szöke for stimulating discussions. These studies were supported by NIH grants GM-17129 and GM-59140, and a grant from the Agouron Institute (to H.F.N.). J.H.C. was supported by a Damon Runyon-Walter Winchell postdoctoral fellowship. The Macromolecular Crystallography Facility at the Advanced Light Source is principally funded by the Office of Biological and Environmental Research of the Department of Energy, with additional funding from the National Institute of General Medical Sciences of the NIH, Lawrence Berkeley National Laboratory, and other participating research team members. The Stanford Synchrotron Radiation Laboratory (SSRL) is funded by the Department of Energy and the NIH, National Center for Research Resources, Biomedical Technology Program. Coordinates for the identified structures of the 70S ribosome have been deposited with the Protein Data Bank (accession number 486D).