Combinatorial protein design by in vitro recombination

Current Opinion in Chemical Biology

Volumn 2, Issue 3, 1998, Pages 335-338

  Giver, Lori ^a   Arnold, Frances H ^a  

^a California Institute of Technology   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DNA; HYBRID PROTEIN; PROTEIN; RECOMBINANT DNA; RECOMBINANT PROTEIN;

BIOSYNTHESIS; CHEMISTRY; DIRECTED MOLECULAR EVOLUTION; DRUG DESIGN; GENETIC RECOMBINATION; GENETIC VARIABILITY; GENETICS; PROTEIN SYNTHESIS; REVIEW;

DIRECTED MOLECULAR EVOLUTION; DNA; DNA, RECOMBINANT; DRUG DESIGN; PROTEIN BIOSYNTHESIS; PROTEINS; RECOMBINANT FUSION PROTEINS; RECOMBINANT PROTEINS; RECOMBINATION, GENETIC; VARIATION (GENETICS);

EID: 0032085975     PISSN: 13675931     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1367-5931(98)80006-9     Document Type: Article

References (26)

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24. Zhao H, Arnold FH: Functional and non-functional mutations distinguished by random recombination of homologous genes. Proc Natl Acad Sci USA 1997, 94:7997-8000. This paper outlines an efficient method for identifying mutations responsible for a difference in the function of two proteins by high-fidelity DNA shuffling.
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26. Zhao H, Giver L, Shao Z, Affholter JA, Arnold FH: Molecular evolution by staggered extension process (StEP) in vitro recombination. Nat Biotechnol 1998, 16:258-261. Describes a method for in vitro recombination based on template switching during DNA synthesis. This technically simple method is used to evolve a thermostable subtilisin.