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1
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0024438708
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Electrospray ionization for mass spectrometry of large biomolecules
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Fenn JB, Mann M, Meng CK, Wong SF, Whitehouse CM. Electrospray ionization for mass spectrometry of large biomolecules. Science. 246:1989;64-71.
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(1989)
Science
, vol.246
, pp. 64-71
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Fenn, J.B.1
Mann, M.2
Meng, C.K.3
Wong, S.F.4
Whitehouse, C.M.5
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2
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0024289037
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Laser desorption ionization of proteins with molecular masses exceeding 10,000 Daltons
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Karas M, Hillenkamp F. Laser desorption ionization of proteins with molecular masses exceeding 10,000 Daltons. Anal Chem. 60:1988;2299-2301.
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Anal Chem
, vol.60
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Karas, M.1
Hillenkamp, F.2
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4
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0028898603
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Characterization of oligonucleotides and nucleic acids by mass spectrometry
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Limbach PA, Crain PF, McCloskey JA. Characterization of oligonucleotides and nucleic acids by mass spectrometry. Curr Opin Biotechnol. 6:1995;96-102.
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(1995)
Curr Opin Biotechnol
, vol.6
, pp. 96-102
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Limbach, P.A.1
Crain, P.F.2
McCloskey, J.A.3
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5
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0031013294
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Mass spectrometry in protein studies from genome to function
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Roepstorff P. Mass spectrometry in protein studies from genome to function. Curr Opin Biotechnol. 8:1997;6-13.
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(1997)
Curr Opin Biotechnol
, vol.8
, pp. 6-13
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Roepstorff, P.1
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6
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6244261674
-
Mass spectrometry of nucleic acids
-
of outstanding interest. This extensive review, with 190 citations, covers the literature until July 1996. Both ESI and MALDI are included, with a section devoted to comparison of some aspects of the two ionization methods, and good summaries of gas phase chemistry including proposed fragmentation mechanisms
-
Nordhoff E, Kirpekar F, Roepstorff P. Mass spectrometry of nucleic acids. of outstanding interest Mass Spectrom Rev. 15:1996;67-138 This extensive review, with 190 citations, covers the literature until July 1996. Both ESI and MALDI are included, with a section devoted to comparison of some aspects of the two ionization methods, and good summaries of gas phase chemistry including proposed fragmentation mechanisms.
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(1996)
Mass Spectrom Rev
, vol.15
, pp. 67-138
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Nordhoff, E.1
Kirpekar, F.2
Roepstorff, P.3
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8
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0029851893
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DNA sequencing by mass spectrometry
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Murray KK. DNA sequencing by mass spectrometry. J Mass Spectrum. 31:1996;1203-1215.
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(1996)
J Mass Spectrum
, vol.31
, pp. 1203-1215
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Murray, K.K.1
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9
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0000390381
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Indirect mass spectrometric methods for characterizing and sequencing oligonucleotides
-
of outstanding interest. A clear presentation of all contemporary MS-based methods proposed for oligonucleotide sequencing, which concentrates on indirect methods involving mass measurement of chain cleavage products formed in solution.
-
Limbach PA. Indirect mass spectrometric methods for characterizing and sequencing oligonucleotides. of outstanding interest Mass Spectrom Rev. 15:1996;297-336 A clear presentation of all contemporary MS-based methods proposed for oligonucleotide sequencing, which concentrates on indirect methods involving mass measurement of chain cleavage products formed in solution.
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(1996)
Mass Spectrom Rev
, vol.15
, pp. 297-336
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-
Limbach, P.A.1
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10
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0029128485
-
Mass spectrometry of nucleic acids: The promise of matrix-assisted laser desorption-ionization (MALDI) mass spectrometry
-
Fitzgerald MC, Smith LM. Mass spectrometry of nucleic acids: the promise of matrix-assisted laser desorption-ionization (MALDI) mass spectrometry. Annu Rev Biophys Biomol Struct. 24:1995;117-140.
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(1995)
Annu Rev Biophys Biomol Struct
, vol.24
, pp. 117-140
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Fitzgerald, M.C.1
Smith, L.M.2
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12
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0028838436
-
Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS)
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Stults JT. Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS). Curr Opin Struct Biol. 5:1995;691-698.
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(1995)
Curr Opin Struct Biol
, vol.5
, pp. 691-698
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Stults, J.T.1
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13
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0000650524
-
An introduction to electrospray ionization and matrix-assisted laser desorption/ionization mass spectrometry: Essential tools in a modern biotechnology environment
-
Hop CECA, Bakhtiar R. An introduction to electrospray ionization and matrix-assisted laser desorption/ionization mass spectrometry: essential tools in a modern biotechnology environment. Biospectroscopy. 3:1997;259-280.
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(1997)
Biospectroscopy
, vol.3
, pp. 259-280
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Hop, C.E.C.A.1
Bakhtiar, R.2
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14
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0000260229
-
Can MS really compete in the DNA world?
-
of specifial interest. A lively summary of viewpoints regarding expectations and reality in the use of MS for DNA sequencing, including large scale efforts (unlikely) and DNA diagnostic (more promising)
-
Henry C. Can MS really compete in the DNA world? of specifial interest Anal Chem. 69:1997;243A-246A A lively summary of viewpoints regarding expectations and reality in the use of MS for DNA sequencing, including large scale efforts (unlikely) and DNA diagnostic (more promising).
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(1997)
Anal Chem
, vol.69
-
-
Henry, C.1
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15
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0030223248
-
Sequence from spectrometry: A realistic prospect?
-
of specifial interest. A brief and readable assessment of the issues facing development of practical MALDI-based sequencing schemes.
-
Smith LM. Sequence from spectrometry: a realistic prospect? of specifial interest Nat Biotechnol. 14:1996;1084-1085 A brief and readable assessment of the issues facing development of practical MALDI-based sequencing schemes.
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(1996)
Nat Biotechnol
, vol.14
, pp. 1084-1085
-
-
Smith, L.M.1
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16
-
-
0030960365
-
Perfect timing: Time-of-flight mass spectrometry
-
of specifial interest. This excellent paper reviews the operating principles and characteristics of the TOF mass analyzer and describes several novel multianalyzer (hybrid) configurations.
-
Gilhaus M, Mlynski V, Selby D. Perfect timing: time-of-flight mass spectrometry. of specifial interest Rapid Commun Mass Spectrom. 11:1997;951-962 This excellent paper reviews the operating principles and characteristics of the TOF mass analyzer and describes several novel multianalyzer (hybrid) configurations.
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(1997)
Rapid Commun Mass Spectrom
, vol.11
, pp. 951-962
-
-
Gilhaus, M.1
Mlynski, V.2
Selby, D.3
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17
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0028836940
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Direct mass spectrometric sequencing of low-picomole amounts of oligodeoxynucleotides with up to 21 bases by matrix-assisted laser desorption/ionization mass spectrometry
-
Nordhoff E, Karas M, Cramer R, Hahner S, Hillenkamp F, Kirpekar F, Lezius A, Muth J, Meier C, Engels JW. Direct mass spectrometric sequencing of low-picomole amounts of oligodeoxynucleotides with up to 21 bases by matrix-assisted laser desorption/ionization mass spectrometry. J Mass Spectrom. 30:1995;99-112.
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(1995)
J Mass Spectrom
, vol.30
, pp. 99-112
-
-
Nordhoff, E.1
Karas, M.2
Cramer, R.3
Hahner, S.4
Hillenkamp, F.5
Kirpekar, F.6
Lezius, A.7
Muth, J.8
Meier, C.9
Engels, J.W.10
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18
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-
0030200945
-
Interpretation of oligonucleotide mass spectra from determination of sequence using electrospray ionization and tandem mass spectrometry
-
of specifial interest. Procedures are described for manual or rapid computer-based interpretation of oligonucleotide mass spectra from collision-induced dissociation. Application to completely unknown sequences and to oligonucleotides modified in the base or sugar are emphasized.
-
Ni J, Pomerantz SC, Rozenski J, Zhang Y, McCloskey JA. Interpretation of oligonucleotide mass spectra from determination of sequence using electrospray ionization and tandem mass spectrometry. of specifial interest Anal Chem. 68:1996;1989-1999 Procedures are described for manual or rapid computer-based interpretation of oligonucleotide mass spectra from collision-induced dissociation. Application to completely unknown sequences and to oligonucleotides modified in the base or sugar are emphasized.
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(1996)
Anal Chem
, vol.68
, pp. 1989-1999
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-
Ni, J.1
Pomerantz, S.C.2
Rozenski, J.3
Zhang, Y.4
McCloskey, J.A.5
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19
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0029638418
-
Oligodeoxynucleotide fragmentation in MALDI/TOF mass spectrometry using 355-nm radiation
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Zhu L, Parr GR, Fitzgerald MC, Nelson CM, Smith LM. Oligodeoxynucleotide fragmentation in MALDI/TOF mass spectrometry using 355-nm radiation. J Am Chem Soc. 117:1995;6048-6056.
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(1995)
J Am Chem Soc
, vol.117
, pp. 6048-6056
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-
Zhu, L.1
Parr, G.R.2
Fitzgerald, M.C.3
Nelson, C.M.4
Smith, L.M.5
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20
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0030917730
-
Oligonucleotide sequencing by fragmentation in matrix-assisted laser desorption/ionization time-of-flight mass spectrometry
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Zhu YF, Taranenko NI, Allman SL, Taranenko NV, Martin SA, Haff LA, Chen CH. Oligonucleotide sequencing by fragmentation in matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Rapid Commmun Mass Spectrom. 11:1997;897-903.
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(1997)
Rapid Commmun Mass Spectrom
, vol.11
, pp. 897-903
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-
Zhu, Y.F.1
Taranenko, N.I.2
Allman, S.L.3
Taranenko, N.V.4
Martin, S.A.5
Haff, L.A.6
Chen, C.H.7
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21
-
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0030977566
-
Deconvolution of combinatorial oligonucleotide libraries by electrospray ionization tandem mass spectrometry
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Pomerantz SC, McCloskey JA, Tarasow T, Eaton BE. Deconvolution of combinatorial oligonucleotide libraries by electrospray ionization tandem mass spectrometry. J Am Chem Soc. 119:1997;3861-3867.
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(1997)
J Am Chem Soc
, vol.119
, pp. 3861-3867
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-
Pomerantz, S.C.1
McCloskey, J.A.2
Tarasow, T.3
Eaton, B.E.4
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22
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0029851586
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The effect of backbone charge on the collision-induced dissociation of oligonucleotides
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Bartlett MG, McCloskey JA, Manalili S, Griffey RH. The effect of backbone charge on the collision-induced dissociation of oligonucleotides. J Mass Spectrom. 31:1996;1277-1283.
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(1996)
J Mass Spectrom
, vol.31
, pp. 1277-1283
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Bartlett, M.G.1
McCloskey, J.A.2
Manalili, S.3
Griffey, R.H.4
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23
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0030786967
-
Collision-induced dissociation of polyprotonated oligonucleotides produced by electrospray ionization
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Ni J, Mathews MAA, McCloskey JA. Collision-induced dissociation of polyprotonated oligonucleotides produced by electrospray ionization. Rapid Commun Mass Spectrom. 11:1997;535-540.
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(1997)
Rapid Commun Mass Spectrom
, vol.11
, pp. 535-540
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-
Ni, J.1
Mathews, M.A.A.2
McCloskey, J.A.3
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24
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0029812647
-
Characterization of peptide oligonucleotide heteroconjugates by mass spectrometry
-
of specifial interest. This paper explores analytical strategies using MALDI and ESI, for detailed sequence analysis of both the peptide and oligonucleotide moieties in a model conjugate (see Figure 1). Although the selection of experimental parameters (e.g. positive versus negative ions) may vary depending on structural features, the data presented illustrate the potential of mass spectrometry in this area.
-
Jensen ON, Kulkarni S, Aldrich JV, Barofsky DF. Characterization of peptide oligonucleotide heteroconjugates by mass spectrometry. of specifial interest Nucleic Acids Res. 24:1996;3866-3872 This paper explores analytical strategies using MALDI and ESI, for detailed sequence analysis of both the peptide and oligonucleotide moieties in a model conjugate (see Figure 1). Although the selection of experimental parameters (e.g. positive versus negative ions) may vary depending on structural features, the data presented illustrate the potential of mass spectrometry in this area.
-
(1996)
Nucleic Acids Res
, vol.24
, pp. 3866-3872
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-
Jensen, O.N.1
Kulkarni, S.2
Aldrich, J.V.3
Barofsky, D.F.4
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25
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0030047433
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DNA sequencing by delayed extraction-matrix-assisted laser desorption/ionization time of flight mass spectrometry
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Roskey MT, Juhasz P, Smimov IP, Takach EJ, Martin SA, Haff LA. DNA sequencing by delayed extraction-matrix-assisted laser desorption/ionization time of flight mass spectrometry. Proc Natl Acad Sci USA. 93:1996;4724-4729.
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(1996)
Proc Natl Acad Sci USA
, vol.93
, pp. 4724-4729
-
-
Roskey, M.T.1
Juhasz, P.2
Smimov, I.P.3
Takach, E.J.4
Martin, S.A.5
Haff, L.A.6
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26
-
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0031003772
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Matrix-assisted laser desorption/ionization for sequencing single-stranded and double-stranded DNA
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Taranenko NI, Chung CN, Zhu YF, Allman SL, Golovlev VV, Isola NR, Martin SA, Haff LA, Chen CH. Matrix-assisted laser desorption/ionization for sequencing single-stranded and double-stranded DNA. Rapid Commun Mass Spectrom. 11:1997;386-392.
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(1997)
Rapid Commun Mass Spectrom
, vol.11
, pp. 386-392
-
-
Taranenko, N.I.1
Chung, C.N.2
Zhu, Y.F.3
Allman, S.L.4
Golovlev, V.V.5
Isola, N.R.6
Martin, S.A.7
Haff, L.A.8
Chen, C.H.9
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27
-
-
0345642483
-
Matrix-assisted laser desorption/ionization mass spectrometry (MALDI) of endonuclease digests of RNA
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Hahner S, Lüdemann H-C, Kirpekar F, Nordhoff E, Roepstorff P, Galla H-J, Hillenkamp F. Matrix-assisted laser desorption/ionization mass spectrometry (MALDI) of endonuclease digests of RNA. Nucleic Acids Res. 25:1997;1957-1964.
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(1997)
Nucleic Acids Res
, vol.25
, pp. 1957-1964
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-
Hahner, S.1
Lüdemann H-C2
Kirpekar, F.3
Nordhoff, E.4
Roepstorff, P.5
Galla H-J6
Hillenkamp, F.7
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28
-
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0029176607
-
Molecular mass measurement of polymerase chain reaction products amplified from human blood DNA by electrospray ionization mass spectrometry
-
Naito Y, Ishikawa K, Koga Y, Tsuneyoshi T, Terunuma H, Arakawa R. Molecular mass measurement of polymerase chain reaction products amplified from human blood DNA by electrospray ionization mass spectrometry. Rapid Commun Mass Spectrom. 9:1995;1484-1486.
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(1995)
Rapid Commun Mass Spectrom
, vol.9
, pp. 1484-1486
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-
Naito, Y.1
Ishikawa, K.2
Koga, Y.3
Tsuneyoshi, T.4
Terunuma, H.5
Arakawa, R.6
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29
-
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0030044298
-
Analysis of double-stranded polymerase chain reaction products from the Bacillus cereus group by electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry
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Wunschel DS, Fox KF, Fox A, Bruce JE, Muddiman DC, Smith RD. Analysis of double-stranded polymerase chain reaction products from the Bacillus cereus group by electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry. Rapid Commun Mass Spectrom. 10:1996;29-35.
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(1996)
Rapid Commun Mass Spectrom
, vol.10
, pp. 29-35
-
-
Wunschel, D.S.1
Fox, K.F.2
Fox, A.3
Bruce, J.E.4
Muddiman, D.C.5
Smith, R.D.6
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30
-
-
0031194993
-
Genetic diagnosis by polymerase chain reaction and electrospray ionization mass spectrometry: Detection of five-base pair deletion from blood DNA of a familial adenomatous polyposis patient
-
Naito Y, Ishikawa K, Koga Y, Tsuneyoshi T, Terunuma H, Arakawa R. Genetic diagnosis by polymerase chain reaction and electrospray ionization mass spectrometry: detection of five-base pair deletion from blood DNA of a familial adenomatous polyposis patient. J Am Soc Mass Spectrom. 8:1997;737-742.
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(1997)
J Am Soc Mass Spectrom
, vol.8
, pp. 737-742
-
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Naito, Y.1
Ishikawa, K.2
Koga, Y.3
Tsuneyoshi, T.4
Terunuma, H.5
Arakawa, R.6
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31
-
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0030019589
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Detection of bacterial DNA polymerase chain reaction products by matrix-assisted laser desorption/ionization mass spectrometry
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Hurst GB, Doktycz MJ, Vass AA, Buchanan MV. Detection of bacterial DNA polymerase chain reaction products by matrix-assisted laser desorption/ionization mass spectrometry. Rapid Commun Mass Spectrom. 10:1996;377-382.
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(1996)
Rapid Commun Mass Spectrom
, vol.10
, pp. 377-382
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Hurst, G.B.1
Doktycz, M.J.2
Vass, A.A.3
Buchanan, M.V.4
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32
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0030004383
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Analysis of ligase chain reaction products via matrix-assisted laser desportion/ionization time-of-flight-mass spectrometry
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Jurinke C, van den Boom D, Jacob A, Tang K, Wörl R, Köster H. Analysis of ligase chain reaction products via matrix-assisted laser desportion/ionization time-of-flight-mass spectrometry. Anal Biochem. 237:1996;174-181.
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(1996)
Anal Biochem
, vol.237
, pp. 174-181
-
-
Jurinke, C.1
Van Den Boom, D.2
Jacob, A.3
Tang, K.4
Wörl, R.5
Köster, H.6
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33
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0030790942
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Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry as a rapid screening method to detect mutations causing Tay-Sachs disease
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Srinivasan JR, Liu Y-H, Venta PJ, Siemieniak D, Killeen AA, Zhu Y, Lubman DM. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry as a rapid screening method to detect mutations causing Tay-Sachs disease. Rapid Commun Mass Spectrom. 11:1997;1144-1150.
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(1997)
Rapid Commun Mass Spectrom
, vol.11
, pp. 1144-1150
-
-
Srinivasan, J.R.1
Liu Y-H2
Venta, P.J.3
Siemieniak, D.4
Killeen, A.A.5
Zhu, Y.6
Lubman, D.M.7
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34
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0030890116
-
Single-nucleotide polymorphism identification assays using a thermostable DNA polymerase and delayed extraction MALDI-TOF mass spectrometry
-
of specifial interest. The PinPoint assay described here probes the identity of a single nucleotide at the location of interest. PCR is initiated with a primer terminating one nucleotide upstream of the target site and four ddNTPs. The primer is elongated by only one residue, corresponding to the base complementary to the site of interest, and gives a short, readily analyzed DNA fragment.
-
Haff LA, Smirnov IP. Single-nucleotide polymorphism identification assays using a thermostable DNA polymerase and delayed extraction MALDI-TOF mass spectrometry. of specifial interest Genome Res. 7:1997;378-388 The PinPoint assay described here probes the identity of a single nucleotide at the location of interest. PCR is initiated with a primer terminating one nucleotide upstream of the target site and four ddNTPs. The primer is elongated by only one residue, corresponding to the base complementary to the site of interest, and gives a short, readily analyzed DNA fragment.
-
(1997)
Genome Res
, vol.7
, pp. 378-388
-
-
Haff, L.A.1
Smirnov, I.P.2
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35
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0031573414
-
Improved analysis of microsatellites using mass spectrometry
-
of specifial interest
-
Braun A, Little DP, Reuter D, Müller-Mysok B, Köster H. Improved analysis of microsatellites using mass spectrometry. of specifial interest Genomics. 46:1997; The generation of informative short PCR products using a modification of the primer oligo base extension protocol is described. By adjusting the dNTP/ddNTP ratio, unexpected second site mutations in could be detected in microsatellite DNA.
-
(1997)
Genomics
, vol.46
-
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Braun, A.1
Little, D.P.2
Reuter, D.3
Müller-Mysok, B.4
Köster, H.5
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36
-
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0031242546
-
Analysis of short tandem repeat polymorphisms in human DNA by matrix-assisted laser desorption/ionization mass spectrometry
-
Ross PL, Belgrader P. Analysis of short tandem repeat polymorphisms in human DNA by matrix-assisted laser desorption/ionization mass spectrometry. Anal Chem. 69:1997;3966-3972.
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(1997)
Anal Chem
, vol.69
, pp. 3966-3972
-
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Ross, P.L.1
Belgrader, P.2
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37
-
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0031572434
-
Discrimination of single-nucleotide polymorphisms in human DNA using peptide nucleic acid probes detected by MALDI-TOF mass spectrometry
-
Ross PL, Lee K, Belgrader P. Discrimination of single-nucleotide polymorphisms in human DNA using peptide nucleic acid probes detected by MALDI-TOF mass spectrometry. Anal Chem. 69:1997;41974202.
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(1997)
Anal Chem
, vol.69
, pp. 41974202
-
-
Ross, P.L.1
Lee, K.2
Belgrader, P.3
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38
-
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0029084910
-
Matrix-assisted laser desorption/ionization mass spectrometry of immobilized duplex DNA probes
-
Tang K, Fu D, Kötter S, Cotter RJ, Cantor CR, Köster H. Matrix-assisted laser desorption/ionization mass spectrometry of immobilized duplex DNA probes. Nucleic Acids Res. 23:1995;3126-3131.
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(1995)
Nucleic Acids Res
, vol.23
, pp. 3126-3131
-
-
Tang, K.1
Fu, D.2
Kötter, S.3
Cotter, R.J.4
Cantor, C.R.5
Köster, H.6
-
39
-
-
0000561129
-
High density, covalent attachment of DNA to silicon wafers for analysis by MALDI-TOF mass spectrometry
-
O'Donnell MJ, Tang K, Köster H, Smith CL, Cantor CR. High density, covalent attachment of DNA to silicon wafers for analysis by MALDI-TOF mass spectrometry. Anal Chem. 69:1997;2438-2443.
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(1997)
Anal Chem
, vol.69
, pp. 2438-2443
-
-
O'Donnell, M.J.1
Tang, K.2
Köster, H.3
Smith, C.L.4
Cantor, C.R.5
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40
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0000184162
-
MALDI on a chip: Analysis of arrays of low-femtomole to subfemtomole quantities of synthetic oligonucleotides and DNA diagnostic products dispensed by a piezoelectric pipet
-
of specifial interest. This paper addresses an important component of protocols under development for high-throughput automated analyses. A piezoelectric pipet is used to dispense low nanoliter volumes of matrix and DNA into individual wells, so that the sample area is approximately the same size as the laser irradiation area. The result is high sensitivity (Figure 2), greater spot-to-spot uniformity, and avoidance of the need to search the target surface for optimal sensitivity regions.
-
Little DP, Cornish TJ, O'Donnell MJ, Braun A, Cotter RJ, Köster H. MALDI on a chip: analysis of arrays of low-femtomole to subfemtomole quantities of synthetic oligonucleotides and DNA diagnostic products dispensed by a piezoelectric pipet. of specifial interest Anal Chem. 69:1997;4540-4546 This paper addresses an important component of protocols under development for high-throughput automated analyses. A piezoelectric pipet is used to dispense low nanoliter volumes of matrix and DNA into individual wells, so that the sample area is approximately the same size as the laser irradiation area. The result is high sensitivity (Figure 2), greater spot-to-spot uniformity, and avoidance of the need to search the target surface for optimal sensitivity regions.
-
(1997)
Anal Chem
, vol.69
, pp. 4540-4546
-
-
Little, D.P.1
Cornish, T.J.2
O'Donnell, M.J.3
Braun, A.4
Cotter, R.J.5
Köster, H.6
-
41
-
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0031081353
-
Multichannel microchip electrospray mass spectrometry
-
Xue Q, Foret F, Dunayevskiy YM, Zavracky PM, McGruer NE, Karger BL. Multichannel microchip electrospray mass spectrometry. Anal Chem. 69:1997;426-430.
-
(1997)
Anal Chem
, vol.69
, pp. 426-430
-
-
Xue, Q.1
Foret, F.2
Dunayevskiy, Y.M.3
Zavracky, P.M.4
McGruer, N.E.5
Karger, B.L.6
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42
-
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0030852959
-
Detecting CFTR gene mutations by using primer oligo base extension and mass spectrometry
-
Braun A, Little DP, Köster H. Detecting CFTR gene mutations by using primer oligo base extension and mass spectrometry. Clin Chem. 43:1997;1151-1158.
-
(1997)
Clin Chem
, vol.43
, pp. 1151-1158
-
-
Braun, A.1
Little, D.P.2
Köster, H.3
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43
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0030863195
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Multiplex genotyping of PCR products with MassTag-labeled primers
-
Haff LA, Smirnov IP. Multiplex genotyping of PCR products with MassTag-labeled primers. Nucleic Acids Res. 25:1997;3749-3750.
-
(1997)
Nucleic Acids Res
, vol.25
, pp. 3749-3750
-
-
Haff, L.A.1
Smirnov, I.P.2
-
44
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0029657614
-
A strategy for rapid and efficient DNA sequencing by mass spectrometry
-
of outstanding interest. This optimistic paper summarizes recent experiments and future plans for the application of MALDI to high-throughput sequencing. Two approaches are described, utilizing immobilized synthetic template or immobilized duplex probes, as elements of a future automated method for mass measurement of sequencing ladders.
-
Köster H, Tang K, Fu D-J, Braun A, van den Boom D, Smith CL, Cotter RJ, Cantor CR. A strategy for rapid and efficient DNA sequencing by mass spectrometry. of outstanding interest Nat Biotechnol. 14:1996;1123-1128 This optimistic paper summarizes recent experiments and future plans for the application of MALDI to high-throughput sequencing. Two approaches are described, utilizing immobilized synthetic template or immobilized duplex probes, as elements of a future automated method for mass measurement of sequencing ladders.
-
(1996)
Nat Biotechnol
, vol.14
, pp. 1123-1128
-
-
Köster, H.1
Tang, K.2
Fu D-J3
Braun, A.4
Van Den Boom, D.5
Smith, C.L.6
Cotter, R.J.7
Cantor, C.R.8
-
45
-
-
0000876636
-
Measurement of macromolecular binding using electrospray mass spectrometry. Determination of dissociation constants for oligonucletide:serum albumin complexes
-
Greig MJ, Gaus H, Cummins LL, Sasmor H, Griffey R. Measurement of macromolecular binding using electrospray mass spectrometry. Determination of dissociation constants for oligonucletide:serum albumin complexes. J Am Chem Soc. 117:1995;10765-10766.
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(1995)
J Am Chem Soc
, vol.117
, pp. 10765-10766
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Greig, M.J.1
Gaus, H.2
Cummins, L.L.3
Sasmor, H.4
Griffey, R.5
-
46
-
-
0030621966
-
Studying noncovalent protein complexes by electrospray ionization mass spectrometry
-
of specifial interest. This extensive review includes protein - nucleic acid complexes and a good discussion of experimental variables, as well as comparison of solution phase versus gas phase binding characteristics, and the complementarity of the ESI technique to various biophysical methods.
-
Loo JA. Studying noncovalent protein complexes by electrospray ionization mass spectrometry. of specifial interest Mass Spectrom Rev. 16:1997;123 This extensive review includes protein - nucleic acid complexes and a good discussion of experimental variables, as well as comparison of solution phase versus gas phase binding characteristics, and the complementarity of the ESI technique to various biophysical methods.
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(1997)
Mass Spectrom Rev
, vol.16
, pp. 123
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Loo, J.A.1
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47
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0001732585
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Specific and nonspecific dimer formation in the electrospray ionization mass spectrometry of oligonucleotides
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Ding J, Anderegg RJ. Specific and nonspecific dimer formation in the electrospray ionization mass spectrometry of oligonucleotides. J Am Soc Mass Spectrom. 6:1995;159-164.
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(1995)
J Am Soc Mass Spectrom
, vol.6
, pp. 159-164
-
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Ding, J.1
Anderegg, R.J.2
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48
-
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0029946594
-
Direct measurement of oligonucleotide binding stoichiometry of gene V protein by mass spectrometry
-
Cheng X, Harms AC, Goudreau PN, Terwilliger TC, Smith RD. Direct measurement of oligonucleotide binding stoichiometry of gene V protein by mass spectrometry. Proc Natl Acad Sci USA. 93:1996;7022-7027.
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(1996)
Proc Natl Acad Sci USA
, vol.93
, pp. 7022-7027
-
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Cheng, X.1
Harms, A.C.2
Goudreau, P.N.3
Terwilliger, T.C.4
Smith, R.D.5
-
49
-
-
0031573676
-
HIV-1 Tat peptide binding to TAR RNA by electrospray ionization mass spectrometry
-
in press
-
Sannes-Lowery KA, Hu P, Mack DP, Mei H-Y, Loo JA. HIV-1 Tat peptide binding to TAR RNA by electrospray ionization mass spectrometry. Anal Chem. 69:1997;. in press.
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(1997)
Anal Chem
, vol.69
-
-
Sannes-Lowery, K.A.1
Hu, P.2
MacK, D.P.3
Mei H-Y4
Loo, J.A.5
-
50
-
-
0030058151
-
Binding specificity of post-activated neocarzinostatin chromophore drug - Bulged DNA complex studied using electrospray ionization mass spectrometry
-
Gao Q, Cheng X, Smith RD, Yang CF, Goldberg IH. Binding specificity of post-activated neocarzinostatin chromophore drug - bulged DNA complex studied using electrospray ionization mass spectrometry. J Mass Spectrom. 31:1996;31-36.
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(1996)
J Mass Spectrom
, vol.31
, pp. 31-36
-
-
Gao, Q.1
Cheng, X.2
Smith, R.D.3
Yang, C.F.4
Goldberg, I.H.5
-
51
-
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0029931505
-
Specific metaloligonucleotide binding studied by high resolution tandem mass spectrometry
-
2+ complexes with oligodeoxynucleotides is used to derive information on binding sites and relative binding specificities of the metal ions. Uncertainties in the validity of this approach vis a vis solution structure clearly remain but this work points the way to additional studies.
-
2+ complexes with oligodeoxynucleotides is used to derive information on binding sites and relative binding specificities of the metal ions. Uncertainties in the validity of this approach vis a vis solution structure clearly remain but this work points the way to additional studies.
-
(1996)
J Mass Spectrom
, vol.31
, pp. 669-675
-
-
Wu, Q.1
Cheng, X.2
Hofstadler, S.A.3
Smith, R.D.4
-
52
-
-
0031389722
-
Detection of base pair mismatches in duplex DNA and RNA oligonucleotides using electrospray mass spectrometry
-
of specifial interest. Innovative experiments show that gas-phase oligonucleotide duplexes that contain a single base pair mismatch undergo selective backbone cleavage at that site upon collisional dissociation. The authors suggest that such measurements could ultimately be used to selectively identify genetic mutations, but other applications, such as probes of secondary structure, might also be possible.
-
Griffey RH, Greig MJ. Detection of base pair mismatches in duplex DNA and RNA oligonucleotides using electrospray mass spectrometry. of specifial interest Proc Int Soc Optical Eng (SPIE). 2985:1997;82-86 Innovative experiments show that gas-phase oligonucleotide duplexes that contain a single base pair mismatch undergo selective backbone cleavage at that site upon collisional dissociation. The authors suggest that such measurements could ultimately be used to selectively identify genetic mutations, but other applications, such as probes of secondary structure, might also be possible.
-
(1997)
Proc Int Soc Optical Eng (SPIE)
, vol.2985
, pp. 82-86
-
-
Griffey, R.H.1
Greig, M.J.2
-
53
-
-
0030994807
-
Analysis of oligonucleotides by HPLC-electrospray ionization mass spectrometry
-
of outstanding interest. The authors attribute the effectiveness of this novel solvent system to the volatility of the hexafluoro-2-propanol buffer constituent, which is preferentially depleted at the droplet surface during desolvation. The pH then increases to -10 so that the oligonucleotide-triethylamine ion pairs dissociate, releasing the oligonucleotide into the gas phase.
-
Apffel A, Chakel JA, Fischer S, Lichtenwalter K, Hancock WS. Analysis of oligonucleotides by HPLC-electrospray ionization mass spectrometry. of outstanding interest Anal Chem. 69:1997;1320-1325 The authors attribute the effectiveness of this novel solvent system to the volatility of the hexafluoro-2-propanol buffer constituent, which is preferentially depleted at the droplet surface during desolvation. The pH then increases to -10 so that the oligonucleotide-triethylamine ion pairs dissociate, releasing the oligonucleotide into the gas phase.
-
(1997)
Anal Chem
, vol.69
, pp. 1320-1325
-
-
Apffel, A.1
Chakel, J.A.2
Fischer, S.3
Lichtenwalter, K.4
Hancock, W.S.5
-
54
-
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0030937876
-
Characterization of oligonucleotide metabolism in vivo via liquid chromatography/electrospray tandem mass spectrometry with a quadrupole ion trap mass spectrometer
-
of outstanding interest of specifial interest. Complete identification of phosphorothioate oligonucleotide metabolites from pig kidney was achieved using LC/ESI tandem mass spectrometry in a quadrupole ion trap mass spectrometer, using an HFIP mobile phase (see Apffel 1997 [53]).
-
Griffey RH, Greig MJ, Gaus HJ, Liu K, Monteith D, Winniman M, Cummins LL. Characterization of oligonucleotide metabolism in vivo via liquid chromatography/electrospray tandem mass spectrometry with a quadrupole ion trap mass spectrometer. of outstanding interest of specifial interest J Mass Spectrom. 32:1997;305-313 Complete identification of phosphorothioate oligonucleotide metabolites from pig kidney was achieved using LC/ESI tandem mass spectrometry in a quadrupole ion trap mass spectrometer, using an HFIP mobile phase (see Apffel 1997 [53]).
-
(1997)
J Mass Spectrom
, vol.32
, pp. 305-313
-
-
Griffey, R.H.1
Greig, M.J.2
Gaus, H.J.3
Liu, K.4
Monteith, D.5
Winniman, M.6
Cummins, L.L.7
-
55
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0029194414
-
Utility of organic bases for improved electrospray mass spectrometry of oligonucleotides
-
Greig M, Griffey RH. Utility of organic bases for improved electrospray mass spectrometry of oligonucleotides. Rapid Commun Mass Spectrum. 9:1995;97-102.
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(1995)
Rapid Commun Mass Spectrum
, vol.9
, pp. 97-102
-
-
Greig, M.1
Griffey, R.H.2
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56
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0001962888
-
Molecular mass measurement of intact ribonucleic acids using electrospray ionization quadrupole mass spectrometry
-
Limbach PA, Crain PF, McCloskey JA. Molecular mass measurement of intact ribonucleic acids using electrospray ionization quadrupole mass spectrometry. J Am Soc Mass Spectrom. 6:1995;27-39.
-
(1995)
J Am Soc Mass Spectrom
, vol.6
, pp. 27-39
-
-
Limbach, P.A.1
Crain, P.F.2
McCloskey, J.A.3
-
57
-
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0042581691
-
Matrix-assisted laser desorption/ionisation mass spectrometry of transfer ribonucleic acids isolated from yeast
-
Gruć-Sovulj I, Lüdemann H-C, Hillenkamp F, Weygand-Durašević I, Kućan Ž, Peter-Katalinić J. Matrix-assisted laser desorption/ionisation mass spectrometry of transfer ribonucleic acids isolated from yeast. Nucleic Acids Res. 25:1997;1859-1861.
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(1997)
Nucleic Acids Res
, vol.25
, pp. 1859-1861
-
-
Gruć-Sovulj, I.1
Lüdemann H-C2
Hillenkamp, F.3
Weygand-Durašević, I.4
Kućan, Ž.5
Peter-Katalinić, J.6
-
58
-
-
0031081755
-
On-line HPLC electrospray mass spectrometry of phosphorothioate oligonucleotide metabolites
-
of outstanding interest of specifial interest. This paper, although written prior to report of the HFIP solvent system described by Apffel et al. 1997 [53], provides an excellent example of the uses of mass spectrometry in the identification of oligonucleotide metabolites, 2-deoxyphosphorothioates in this case. The distribution of metabolites in rat plasma, liver and kidney were determined, and were consistent with exonuclease degradation, (primarily) at the 3′-termini.
-
Gaus HJ, Owens SR, Winniman M, Cooper S, Cummins LL. On-line HPLC electrospray mass spectrometry of phosphorothioate oligonucleotide metabolites. of outstanding interest of specifial interest Anal Chem. 69:1997;313-319 This paper, although written prior to report of the HFIP solvent system described by Apffel et al. 1997 [53], provides an excellent example of the uses of mass spectrometry in the identification of oligonucleotide metabolites, 2-deoxyphosphorothioates in this case. The distribution of metabolites in rat plasma, liver and kidney were determined, and were consistent with exonuclease degradation, (primarily) at the 3′-termini.
-
(1997)
Anal Chem
, vol.69
, pp. 313-319
-
-
Gaus, H.J.1
Owens, S.R.2
Winniman, M.3
Cooper, S.4
Cummins, L.L.5
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59
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0029783865
-
On-line microdialysis sample cleanup for electrospray ionization mass spectrometry of nucleic acid samples
-
Liu C, Wu Q, Harms AC, Smith RD. On-line microdialysis sample cleanup for electrospray ionization mass spectrometry of nucleic acid samples. Anal Chem. 68:1996;3295-3299.
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(1996)
Anal Chem
, vol.68
, pp. 3295-3299
-
-
Liu, C.1
Wu, Q.2
Harms, A.C.3
Smith, R.D.4
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60
-
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0030076033
-
Effect of impurities on the matrix-assisted laser desorption mass spectra of single-stranded oligodeoxynucleotides
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Shaler TA, Wickham JN, Sannes KA, Wu KJ, Becker CH. Effect of impurities on the matrix-assisted laser desorption mass spectra of single-stranded oligodeoxynucleotides. Anal Chem. 68:1996;576-579.
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(1996)
Anal Chem
, vol.68
, pp. 576-579
-
-
Shaler, T.A.1
Wickham, J.N.2
Sannes, K.A.3
Wu, K.J.4
Becker, C.H.5
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61
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0030788454
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The use of a co-matrix for improved analysis of oligonucleotides by matrix-assisted laser desorption/ionization time of-flight mass spectrometry
-
Simmons TA, Limbach PA. The use of a co-matrix for improved analysis of oligonucleotides by matrix-assisted laser desorption/ionization time of-flight mass spectrometry. Rapid Commun Mass Spectrom. 11:1997;567-572.
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(1997)
Rapid Commun Mass Spectrom
, vol.11
, pp. 567-572
-
-
Simmons, T.A.1
Limbach, P.A.2
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62
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0030797594
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Influence of solvents and detergents on matrix-assisted laser desorption/ionization mass spectrometry measurements of proteins and oligonucleotides
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Börnsen KO, Gass MAS, Bruin GJM, von Adrichem JHM, Biro MC, Kresbach GM, Ehrat M. Influence of solvents and detergents on matrix-assisted laser desorption/ionization mass spectrometry measurements of proteins and oligonucleotides. Rapid Commun Mass Spectrom. 11:1997;603-609.
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(1997)
Rapid Commun Mass Spectrom
, vol.11
, pp. 603-609
-
-
Börnsen, K.O.1
Gass, M.A.S.2
Bruin, G.J.M.3
Von Adrichem, J.H.M.4
Biro, M.C.5
Kresbach, G.M.6
Ehrat, M.7
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63
-
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0031230725
-
Cryogenic frozen solution matrixes for analysis of DNA by time-of-flight mass spectrometry
-
Hunter JM, Lin H, Becker CH. Cryogenic frozen solution matrixes for analysis of DNA by time-of-flight mass spectrometry. Anal Chem. 69:1997;3608-3612.
-
(1997)
Anal Chem
, vol.69
, pp. 3608-3612
-
-
Hunter, J.M.1
Lin, H.2
Becker, C.H.3
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64
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0031081531
-
Controlling DNA fragmentation in MALDI-MS by chemical modification
-
Tang W, Zhu L, Smith LM. Controlling DNA fragmentation in MALDI-MS by chemical modification. Anal Chem. 69:1997;302-312.
-
(1997)
Anal Chem
, vol.69
, pp. 302-312
-
-
Tang, W.1
Zhu, L.2
Smith, L.M.3
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65
-
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0031052076
-
Analysis of DNA by charge tagging and matrix-assisted laser desorption/ionization mass spectrometry
-
Gut IG, Jeffrey WA, Pappin DJC, Beck S. Analysis of DNA by charge tagging and matrix-assisted laser desorption/ionization mass spectrometry. Rapid Commun Mass Spectrom. 11:1997;43-50.
-
(1997)
Rapid Commun Mass Spectrom
, vol.11
, pp. 43-50
-
-
Gut, I.G.1
Jeffrey, W.A.2
Pappin, D.J.C.3
Beck, S.4
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66
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0345165415
-
Delayed extraction matrix-assisted laser desorption time-of-flight mass spectrometry
-
Vestal ML, Juhasz P, Martin SA. Delayed extraction matrix-assisted laser desorption time-of-flight mass spectrometry. Rapid Commun Mass Spectrom. 9:1995;1044-1050.
-
(1995)
Rapid Commun Mass Spectrom
, vol.9
, pp. 1044-1050
-
-
Vestal, M.L.1
Juhasz, P.2
Martin, S.A.3
-
67
-
-
0028873832
-
High resolution matrix-assisted laser desorption/ionization time-of-flight analysis of single-stranded DNA of 27 to 68 nucleotides in length
-
Christian NP, Colby SM, Giver L, Houston CT, Arnold RJ, Ellington AD, Reilly JP. High resolution matrix-assisted laser desorption/ionization time-of-flight analysis of single-stranded DNA of 27 to 68 nucleotides in length. Rapid Commun Mass Spectrom. 9:1995;1061-1066.
-
(1995)
Rapid Commun Mass Spectrom
, vol.9
, pp. 1061-1066
-
-
Christian, N.P.1
Colby, S.M.2
Giver, L.3
Houston, C.T.4
Arnold, R.J.5
Ellington, A.D.6
Reilly, J.P.7
-
68
-
-
0029927530
-
Applications of delayed extraction matrix-assisted laser desorption ionization time-of-flight mass spectrometry to oligonucleotide analysis
-
of outstanding interest. The authors report notable improvements in instrument performance in the linear, and in particular the reflector mode, of operation when using delayed extraction techniques, demonstrated up to 50 nucleotides in length.
-
Juhasz P, Roskey MT, Smirnov IP, Haff LA, Vestal ML, Martin SA. Applications of delayed extraction matrix-assisted laser desorption ionization time-of-flight mass spectrometry to oligonucleotide analysis. of outstanding interest Anal Chem. 68:1996;941-946 The authors report notable improvements in instrument performance in the linear, and in particular the reflector mode, of operation when using delayed extraction techniques, demonstrated up to 50 nucleotides in length.
-
(1996)
Anal Chem
, vol.68
, pp. 941-946
-
-
Juhasz, P.1
Roskey, M.T.2
Smirnov, I.P.3
Haff, L.A.4
Vestal, M.L.5
Martin, S.A.6
-
69
-
-
0029717657
-
High sensitivity collisionally activated decomposition tandem mass spectrometry on a novel quadrupole/orthogonal-acceleration time-of-flight mass spectrometer
-
of specifial interest. The instrument (Q-TOF) described consists of a quadrupole mass filter, hexapole collision cell and time-of-flight mass analyzer with reflection. Using ESI, sequencing of peptides in low femtomole to attomole amounts was demonstrated. These initial sensitivity results suggest a favorable future for this instrument design in a range of applications.
-
Morris HR, Paxton T, Dell A, Langhorne J, Berg M, Bordoli RS, Hoyes J, Bateman RH. High sensitivity collisionally activated decomposition tandem mass spectrometry on a novel quadrupole/orthogonal-acceleration time-of-flight mass spectrometer. of specifial interest Rapid Commun Mass Spectrom. 10:1996;889-896 The instrument (Q-TOF) described consists of a quadrupole mass filter, hexapole collision cell and time-of-flight mass analyzer with reflection. Using ESI, sequencing of peptides in low femtomole to attomole amounts was demonstrated. These initial sensitivity results suggest a favorable future for this instrument design in a range of applications.
-
(1996)
Rapid Commun Mass Spectrom
, vol.10
, pp. 889-896
-
-
Morris, H.R.1
Paxton, T.2
Dell, A.3
Langhorne, J.4
Berg, M.5
Bordoli, R.S.6
Hoyes, J.7
Bateman, R.H.8
-
70
-
-
0029685702
-
Analytical properties of the nanoelectrospray ion source
-
of specifial interest. This paper discusses the theory and practical operating aspects of the nanospray ion source. This ion source utilizes very small diameter electrospray capillary needles (1-2 μm inner diameter), which are loaded with less than 1 μl of sample solution and operate without necessity of a solvent pump. In general, the operation of the device becomes less routine, compared with the somewhat larger scale microspray ion source (see Limbach et al. 1995 [4]), as the flow rate and orifice diameter are decreased.
-
Wilm M, Mann M. Analytical properties of the nanoelectrospray ion source. of specifial interest Anal Chem. 68:1996;1-8 This paper discusses the theory and practical operating aspects of the nanospray ion source. This ion source utilizes very small diameter electrospray capillary needles (1-2 μm inner diameter), which are loaded with less than 1 μl of sample solution and operate without necessity of a solvent pump. In general, the operation of the device becomes less routine, compared with the somewhat larger scale microspray ion source (see Limbach et al. 1995 [4]), as the flow rate and orifice diameter are decreased.
-
(1996)
Anal Chem
, vol.68
, pp. 1-8
-
-
Wilm, M.1
Mann, M.2
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71
-
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0029645626
-
Attomole-sensitivity electrospray source for large-molecule mass spectrometry
-
Valaskovic GA, Kelleher NL, Little DP, Aaserud DJ, McLafferty FW. Attomole-sensitivity electrospray source for large-molecule mass spectrometry. Anal Chem. 87:1995;3802-3805.
-
(1995)
Anal Chem
, vol.87
, pp. 3802-3805
-
-
Valaskovic, G.A.1
Kelleher, N.L.2
Little, D.P.3
Aaserud, D.J.4
McLafferty, F.W.5
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72
-
-
9344248022
-
Molecular weight determination of plasmid DNA using electrospray ionization mass spectrometry
-
Cheng X, Camp IDG II, Wu Q, Bakhtiar R, Springer DL, Morris BJ, Bruce JE, Anderson GA, Edmonds CG, Smith RD. Molecular weight determination of plasmid DNA using electrospray ionization mass spectrometry. Nucleic Acids Res. 24:1996;2183-2189.
-
(1996)
Nucleic Acids Res
, vol.24
, pp. 2183-2189
-
-
Cheng, X.1
Camp I.D.G. II2
Wu, Q.3
Bakhtiar, R.4
Springer, D.L.5
Morris, B.J.6
Bruce, J.E.7
Anderson, G.A.8
Edmonds, C.G.9
Smith, R.D.10
-
73
-
-
0001424473
-
A gated electrostatic ion trap to repetitiously measure the charge and m/z of large electrospray ions
-
Benner WH. A gated electrostatic ion trap to repetitiously measure the charge and m/z of large electrospray ions. Anal Chem. 69:1997;4162-4168.
-
(1997)
Anal Chem
, vol.69
, pp. 4162-4168
-
-
Benner, W.H.1
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74
-
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0029129615
-
Investigation of the 'n - 1' impurity in phosphorothioate oligodeoxynucleotides synthesized by the solid-phase beta-cyanoethyl phosphoramidite method using stepwise sulfurization
-
Fearon KL, Stults JT, Bergot BJ, Christensen LM, Raible AM. Investigation of the 'n - 1' impurity in phosphorothioate oligodeoxynucleotides synthesized by the solid-phase beta-cyanoethyl phosphoramidite method using stepwise sulfurization. Nucleic Acids Res. 23:1995;2754-2761.
-
(1995)
Nucleic Acids Res
, vol.23
, pp. 2754-2761
-
-
Fearon, K.L.1
Stults, J.T.2
Bergot, B.J.3
Christensen, L.M.4
Raible, A.M.5
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75
-
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0030182916
-
Mass spectrometric investigation of the reaction between 4,4-vinylenedipyridine bis[2,2:6,2-terpyridine platinum (II)] and the self-complementary oligonucleotide d(CpGpTpApCpG)
-
2+, which neutralizes the anionic phosphodiester at the site of adduction.
-
2+, which neutralizes the anionic phosphodiester at the site of adduction.
-
(1996)
Bioorg Med Chem
, vol.4
, pp. 1007-1013
-
-
Lowe, G.1
McCloskey, J.A.2
Ni, J.3
Vilavian, T.4
-
77
-
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0029883720
-
+-1915 in domain IV from E. coli 23S ribosomal RNA as 3-methylpseudouridine
-
of specifial interest. Example of a protocol for location and characterization of modified residues in large RNAs, based on recognition of modifications in RNase T1 hydrolysis products, from mass increments (e.g. 14 Da for methyl) not allowed by the corresponding gene sequence.
-
+-1915 in domain IV from E. coli 23S ribosomal RNA as 3-methylpseudouridine. of specifial interest Nucleic Acids Res. 24:1996;688-693 Example of a protocol for location and characterization of modified residues in large RNAs, based on recognition of modifications in RNase T1 hydrolysis products, from mass increments (e.g. 14 Da for methyl) not allowed by the corresponding gene sequence.
-
(1996)
Nucleic Acids Res
, vol.24
, pp. 688-693
-
-
Kowalak, J.A.1
Bruenger, E.2
Hashizume, T.3
Peltier, J.M.4
Ofengand, J.5
McCloskey, J.A.6
-
78
-
-
0030940842
-
Identification and sequence analysis of contact sites between ribosomal proteins and rRNA in Escherichia coli 30 S subunits by a new approach using matrix-assisted laser desorption/ionization-mass spectrometry combined with N-terminal microsequencing
-
of specifial interest. This excellent paper demonstrates the considerable power of mass spectrometry for the determination of nucleic acid - protein cross-link sites. Peptide-oligonucleotide complexes formed by protease-RNase treatments were isolated by HPLC. The RNA 8-mer linked product was sequenced by measurement of a mass ladder generated by partial alkaline hydrolysis.
-
Urlaub H, Thiede B, Müller E-C, Brimacombe R, Wittmann-Liebold B. Identification and sequence analysis of contact sites between ribosomal proteins and rRNA in Escherichia coli 30 S subunits by a new approach using matrix-assisted laser desorption/ionization-mass spectrometry combined with N-terminal microsequencing. of specifial interest J Biol Chem. 272:1997;14547-14555 This excellent paper demonstrates the considerable power of mass spectrometry for the determination of nucleic acid - protein cross-link sites. Peptide-oligonucleotide complexes formed by protease-RNase treatments were isolated by HPLC. The RNA 8-mer linked product was sequenced by measurement of a mass ladder generated by partial alkaline hydrolysis.
-
(1997)
J Biol Chem
, vol.272
, pp. 14547-14555
-
-
Urlaub, H.1
Thiede, B.2
Müller E-C3
Brimacombe, R.4
Wittmann-Liebold, B.5
-
79
-
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0031040257
-
Lysine 207 as the site of cross-linking between the 3′-end of Escherichia coli initiator tRNA and methionyl-tRNA formyltransferase
-
Gite S, RajBhandary UL. Lysine 207 as the site of cross-linking between the 3′-end of Escherichia coli initiator tRNA and methionyl-tRNA formyltransferase. J Biol Chem. 272:1997;5305-5312.
-
(1997)
J Biol Chem
, vol.272
, pp. 5305-5312
-
-
Gite, S.1
Rajbhandary, U.L.2
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