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Identification and characterization of Drosophila relatives of the yeast transcriptional activator SNF2/SWI2
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Ito T, Bulger M, Pazin MJ, Kobayashi R, Kadonaga JT. ACF, an ISWI-containing and ATP-utilizing chromatin assembly and remodeling factor. of special interest Cell. 90:1997;145-155 ACF, an ATP-utilizing chromatin assembly and remodeling factor, contains ISWI and three additional polypeptides. In a chromatin assembly system consisting of DNA, histones, and a histone chaperone, ACF functions as a catalytic nucleosome-spacing factor. ACF may also mediate promoter-specific nucleosome reconfiguration by Gal4-VP16 in an ATP-dependent manner.
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Ito, T.1
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Crystal structure of the nucleosome core particle at 2.8 Å resolution
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Luger, K.1
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of special interest. A mutant of the thyroid hormone receptor binds thyroid hormone and its heterodimerization partner and creates a local DNase I hypersensitive chromatin perturbation, just like the wild-type receptor. However, in contrast to the wild type, it cannot cause widespread chromatin disruption and transcriptional activation after hormone addition.
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Wong J, Shi Y-B, Wolffe AP. Determinants of chromatin disruption and transcriptional regulation instigated by the thyroid hormone receptor: hormone-regulated chromatin disruption is not sufficient for transcriptional activation. of special interest EMBO J. 16:1997;3158-3171 A mutant of the thyroid hormone receptor binds thyroid hormone and its heterodimerization partner and creates a local DNase I hypersensitive chromatin perturbation, just like the wild-type receptor. However, in contrast to the wild type, it cannot cause widespread chromatin disruption and transcriptional activation after hormone addition.
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Yeast Gcn5 functions in two multisubunit complexes to acetylate nucleosomal histones: Characterization of an Ada complex and the SAGA (Spt/Ada) complex
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of special interest. Gcn5p, the first nuclear histone acetyltransferase to be identified, is part of the SAGA complex that includes proteins that are linked to transcriptional activation: ADA2, ADA3, ADA5/ SPT20, SPT7. This complex acetylates histones within the nucleosome.
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Targeted histone acetyltransferase activity of yeast Gcn5p is required for the activation of downstream genes in vivo
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of special interest. Gcn5p functions as a histone acetyltransferase in vivo, and this histone acetyltransferase activity is necessary for the transcriptional activation of target genes. Gcn5p is responsible for the hyperacetylation of histones H3 and H4 of the immediate promoter region of the target gene.
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Kuo M-H, Zhou J, Jambeck P, Churchill MEA, Allis CD. Targeted histone acetyltransferase activity of yeast Gcn5p is required for the activation of downstream genes in vivo. of special interest Genes Dev. 12:1998;627-639 Gcn5p functions as a histone acetyltransferase in vivo, and this histone acetyltransferase activity is necessary for the transcriptional activation of target genes. Gcn5p is responsible for the hyperacetylation of histones H3 and H4 of the immediate promoter region of the target gene.
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