Microtubule minus ends can be labelled with a phage display antibody specific to alpha-tubulin

Journal of Molecular Biology

Volumn 259, Issue 3, 1996, Pages 325-330

  Fan, Juan ^a   Griffiths, Andrew D ^a   Lockhart, Andrew ^b   Cross, Robert A ^b   Amos, Linda A ^a  

^a MRC LABORATORY OF MOLECULAR BIOLOGY   (United Kingdom)

^b MARIE CURIE RESEARCH INSTITUTE   (United Kingdom)

Author keywords

Microtubules; Phage display antibody; Polarity; Tubulin

Indexed keywords

ALPHA TUBULIN; ANTIBODY; BRAIN PROTEIN; EPITOPE;

AMINO TERMINAL SEQUENCE; ANTIGEN BINDING; ARTICLE; CONTROLLED STUDY; ELECTRON MICROSCOPY; MICROTUBULE; MOLECULAR CLONING; NONHUMAN; PHAGE DISPLAY; PRIORITY JOURNAL; PROTEIN EXPRESSION; SEA URCHIN;

ECHINOIDEA;

EID: 0029887264     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1006/jmbi.1996.0322     Document Type: Editorial

References (19)

1. Amos, L. A. (1995). The microtubule lattice: 20 years on. Trends Cell Biol. 5, 48-51.
2. Griffiths, A. D., Williams, S. C., Hartley, O., Tomlinson, I. M., Waterhouse, P., Crosby W. L., Kontermann, E., Jones, P. T., Low, N. M., Allison, T. J., Prospero, T. D., Hoogenboom, H. R., Nissim, A., Cox, J. P. L., Harrison, J. L., Zaccolo, M., Gherardi, E. & Winter, G. (1994). Isolation of high affinity human antibodies directly from large synthetic repertoires. EMBO J. 13, 3245-3260.
3. Hirose, K., Fan, J. & Amos, L. A. (1995a). Re-examination of the polarity of microtubules and sheets decorated with kinesin motor domain. J. Mol. Biol. 251, 329-333.
4. Hirose, K., Lockhart, A., Cross, R. A. & Amos, L. A. (1995b). Nucleotide-dependent angular change in kinesin motor domain bound to tubulin. Nature, 376, 277-279.
5. Hoenger, A., Sablin, E. P., Vale, R. D., Fletterick, R. J. & Milligan, R. A. (1995). 3-Dimensional structure of a tubulin-motor-protein complex. Nature, 376, 271-274.
6. Huitorel, P. & Kirschner, M. W. (1988). The polarity and stability of microtubule capture by the kinetochore. J. Cell Biol. 106, 151-160.
7. Hyman, A., Drechsel, D., Kellogg, D., Salser, S., Sawin, K., Steffen, P., Wordeman, L. & Mitchison, T. (1991). Preparation of modified tubulins. Methods Enzymol. 196, 478-485.
8. Janknecht, R., de Martynoff, G., Lou, R. A., Nordheim, A. & Stunnenberg, H. G. (1991). Rapid and efficient purification of native histidine-tagged protein expressed by recombinant vaccinia virus. Proc. Natl Acad. Sci. USA, 88, 8972-8976.
9. Lockhart, A. & Cross, R. A. (1996). Kinetics and motility of the Eg5 microtubule motor. Biochemistry. 35, 2365-2373.
10. Luduena, R., Shooter, E. M. & Wilson, L. (1977). Structure of the tubulin dimer. J. Biol. Chem. 252, 7006-7014.
11. Mandelkow, E. & Mandelkow, E.-M. (1989). Tubulin, microtubules and oligomers: molecular structure and implications for assembly. In Cell Movement (Warner, F. D. & McIntosh, J. R., eds), pp. 23-45, Alan R. Liss, New York.
12. Marks, J. D., Hoogenboom, H. R., Bonnert, T. P., McCafferty, J., Griffiths, A. D. & Winter, G. (1991). By-passing immunization. Human antibodies from V-gene libraries displayed on phage. J. Mol. Biol. 222, 581-597.
13. Mitchison, T. J. (1993). Localization of an exchangeable GTP-binding site at the plus end of microtubules. Science, 261, 1044-1047.
14. Munro, S. & Pelham, H. R. B. (1986). An hsp70-like protein in the ER: identity with the 78kd glucose-regulated protein and immunoglobulin heavy chain binding protein. Cell, 46, 291-300.
15. Nogales, E., Wolf, S. G., Khan, I. A., Luduena, R. F. & Downing, K. H. (1995). Structure of tubulin at 6.5 Å and location of the taxol-binding site. Nature, 375, 424-427.
16. Oakley C. E. & Oakley, B. R. (1989). Identification of gamma-tubulin a new member of the tubulin superfamily encoded by mipA gene of Aspergillus nidulans. Nature, 338, 662-664.
17. Sawin, K. E. & Mitchison, T. J. (1995). Mutations in the kinesin-like protein eg5 disrupting localization to the mitotic spindle. Proc. Natl Acad. Sci. USA, 92, 4289-4293.
18. Song, Y.-H. & Mandelkow, E. (1995). The anatomy of flagellar microtubules: polarity seam, junctions, and lattice. J. Cell Biol. 128, 81-94.
19. Vaughan, T. J., Williams, A. J., Pritchard, K., Osbourn, J. K., Pope, A. R., Earnshaw, J. C., McCafferty J., Wilton, J. & Johnson, K. S. (1996). Isolation of human antibodies with sub-nanomolar affinities directly from a large non-immunized phage display library. Nature Biotechnol. 14, 309-314.