-
1
-
-
0003047034
-
-
Oxford: Elsevier Science Ltd.
-
Guengerich FP (Ed): Biotransformation. Oxford: Elsevier Science Ltd.; 1997. [Sipes IG, McQueen CA, Gandolfi AJ (Series Editors): Comprehensive Toxicology, vol 3.] Individual chapters deal with the enzymes discussed here and their significance.
-
(1997)
Biotransformation
-
-
Guengerich, F.P.1
-
2
-
-
85030307124
-
-
Guengerich FP (Ed): Biotransformation. Oxford: Elsevier Science Ltd.; 1997. [Sipes IG, McQueen CA, Gandolfi AJ (Series Editors): Comprehensive Toxicology, vol 3.] Individual chapters deal with the enzymes discussed here and their significance.
-
Comprehensive Toxicology
, vol.3
-
-
Sipes, I.G.1
McQueen, C.A.2
Gandolfi, A.J.3
-
3
-
-
0030294687
-
Eukaryotic expression systems: A comparison
-
Geisse S, Gram H, Kleuser B, Kocher HP: Eukaryotic expression systems: a comparison. Protein Express Purif 1996, 8:271-282. General information is provided about relative merits of different systems.
-
(1996)
Protein Express Purif
, vol.8
, pp. 271-282
-
-
Geisse, S.1
Gram, H.2
Kleuser, B.3
Kocher, H.P.4
-
4
-
-
0027321555
-
Expression of mammalian glutathione S-transferase 5-5 in Salmonella typhimurium TA1535 leads to base-pair mutations upon exposure to dihalomethanes
-
Thier R, Pemble SE, Taylor JB, Humphreys WG, Persmark M, Ketterer B, Guengerich FP: Expression of mammalian glutathione S-transferase 5-5 in Salmonella typhimurium TA1535 leads to base-pair mutations upon exposure to dihalomethanes. Proc Natl Acad Sci USA 1993, 90:8576-8580.
-
(1993)
Proc Natl Acad Sci USA
, vol.90
, pp. 8576-8580
-
-
Thier, R.1
Pemble, S.E.2
Taylor, J.B.3
Humphreys, W.G.4
Persmark, M.5
Ketterer, B.6
Guengerich, F.P.7
-
5
-
-
0030175041
-
Optimized heterologous expression of the polymorphic human glutathione transferase M1-1 based on silent mutations in the corresponding cDNA
-
Widersten M, Huang M, Mannervik B: Optimized heterologous expression of the polymorphic human glutathione transferase M1-1 based on silent mutations in the corresponding cDNA. Protein Expr Purif 1996, 7:367-372. Human glutathione S transferase M1-1 expression in E. coli was improved by random mutagenesis of the 5′ region of the cDNA. Highly-expressing clones were selected by colony immunoblotting. A generally applicable method is presented for improving expression of otherwise poorly expressed enzymes, particularly for large-scale production.
-
(1996)
Protein Expr Purif
, vol.7
, pp. 367-372
-
-
Widersten, M.1
Huang, M.2
Mannervik, B.3
-
6
-
-
0029934241
-
Heterologous expression, purification and characterization of rat class theta glutathione transferase T2-2
-
Jemth P, Stenberg G, Chaga G, Mannervik B: Heterologous expression, purification and characterization of rat class theta glutathione transferase T2-2. Biochem J 1996, 316:131-136.
-
(1996)
Biochem J
, vol.316
, pp. 131-136
-
-
Jemth, P.1
Stenberg, G.2
Chaga, G.3
Mannervik, B.4
-
7
-
-
0029159746
-
Forced evolution of glutathione S-transferase to create a more efficient drug detoxication enzyme
-
Gulick AM, Fahl WE: Forced evolution of glutathione S-transferase to create a more efficient drug detoxication enzyme. Proc Natl Acad Sci USA 1995, 92:8140-8144.
-
(1995)
Proc Natl Acad Sci USA
, vol.92
, pp. 8140-8144
-
-
Gulick, A.M.1
Fahl, W.E.2
-
8
-
-
0029008514
-
Glutathione transferases with novel active sites isolated by phage display from a library of random mutants
-
Widersten M, Mannervik B: Glutathione transferases with novel active sites isolated by phage display from a library of random mutants. J Mol Biol 1995, 250:115-122.
-
(1995)
J Mol Biol
, vol.250
, pp. 115-122
-
-
Widersten, M.1
Mannervik, B.2
-
9
-
-
0030133645
-
Glubodies: Randomized libraries of glutathione transferase enzymes
-
Napolitano EW, Villar HO, Kauvar LM, Higgins DL, Roberts D, Mandac J, Lee SK, Engqvist-Goldstein Å, Bukar R, Calio BL et al.: Glubodies: randomized libraries of glutathione transferase enzymes. Chem Biol 1996, 3:359-367. A generalized strategy for molecular engineering of a protein, particularly for drug-metabolizing enzymes, can be extrapolated from this work with glutathione S transferase. Two domains that interact with ligand were identified by screening bacterial extracts for enzymatic activities.
-
(1996)
Chem Biol
, vol.3
, pp. 359-367
-
-
Napolitano, E.W.1
Villar, H.O.2
Kauvar, L.M.3
Higgins, D.L.4
Roberts, D.5
Mandac, J.6
Lee, S.K.7
Engqvist-Goldstein, Å.8
Bukar, R.9
Calio, B.L.10
-
10
-
-
12644302182
-
Unexpected genetic and structural relationships of a long-forgotten flavoenzyme to NAD(P)H:quinone reductase (DT-diaphorase)
-
Zhao Q, Yang XL, Holtzclaw WD, Talalay P: Unexpected genetic and structural relationships of a long-forgotten flavoenzyme to NAD(P)H:quinone reductase (DT-diaphorase). Proc Natl Acad Sci USA 1997, 94:1669-1674.
-
(1997)
Proc Natl Acad Sci USA
, vol.94
, pp. 1669-1674
-
-
Zhao, Q.1
Yang, X.L.2
Holtzclaw, W.D.3
Talalay, P.4
-
11
-
-
0027298873
-
Human thiopurine methyltransferase: Molecular cloning and expression of T84 colon carcinoma cell cDNA
-
Honchel R, Aksoy IA, Szumlanski C, Wood TC, Otterness DM, Wieben ED, Weinshilboum RM: Human thiopurine methyltransferase: molecular cloning and expression of T84 colon carcinoma cell cDNA. Mol Pharmacol 1993, 43:878-887.
-
(1993)
Mol Pharmacol
, vol.43
, pp. 878-887
-
-
Honchel, R.1
Aksoy, I.A.2
Szumlanski, C.3
Wood, T.C.4
Otterness, D.M.5
Wieben, E.D.6
Weinshilboum, R.M.7
-
12
-
-
0029558167
-
Salmonella strains and mammalian cells genetically engineered for expression of sulfotransferases
-
Glatt H, Bartsch I, Czich A, Seidel A, Falany CN: Salmonella strains and mammalian cells genetically engineered for expression of sulfotransferases. Toxicol Lett 1995, 82/83:829-834.
-
(1995)
Toxicol Lett
, vol.82-83
, pp. 829-834
-
-
Glatt, H.1
Bartsch, I.2
Czich, A.3
Seidel, A.4
Falany, C.N.5
-
13
-
-
0026695811
-
Salmonella typhimurium strains expressing human arylamine N-acetyl transferases: Metabolism and mutagenic activation of aromatic amines
-
Grant DM, Josephy PD, Lord HL, Morrison LD: Salmonella typhimurium strains expressing human arylamine N-acetyl transferases: metabolism and mutagenic activation of aromatic amines. Cancer Res 1992, 52:3961-3964.
-
(1992)
Cancer Res
, vol.52
, pp. 3961-3964
-
-
Grant, D.M.1
Josephy, P.D.2
Lord, H.L.3
Morrison, L.D.4
-
14
-
-
0030019667
-
Arylamine N-acetyltransferases. Expression in Escherichia coli, purification, and substrate specificities of recombinant hamster monomorphic and polymorphic isozymes
-
Wagner CR, Bergstrom CP, Koning KR, Hanna PE: Arylamine N-acetyltransferases. Expression in Escherichia coli, purification, and substrate specificities of recombinant hamster monomorphic and polymorphic isozymes. Drug Metab Dispos 1996, 24:245-253. Two isoforms of arylamine N-acteyl transferase were individually expressed in E. coli as amino-terminal fusions with a FLAG peptide tag (encoding an enterokinase-sensitive site), which facilitated immunoaffinity purification. Removal of this tag with enterokinase, however, resulted in degradation of the protein. Introduction of a thrombin-sensitive site in this region by recombinant DNA methodology permitted cleavage of the immunoreactive peptide by thrombin, yielding the native enzyme.
-
(1996)
Drug Metab Dispos
, vol.24
, pp. 245-253
-
-
Wagner, C.R.1
Bergstrom, C.P.2
Koning, K.R.3
Hanna, P.E.4
-
15
-
-
0026760509
-
Baculovirus expression and characterization of catalytically active horseradish peroxidase
-
Hartmann C, Ortiz de Montellano PR: Baculovirus expression and characterization of catalytically active horseradish peroxidase. Arch Biochem Biophys 1992, 297:61-72.
-
(1992)
Arch Biochem Biophys
, vol.297
, pp. 61-72
-
-
Hartmann, C.1
De Ortiz Montellano, P.R.2
-
16
-
-
0343553140
-
Peroxidases
-
Edited by Guengerich FP. Oxford: Elsevier Science Ltd.
-
Marnett LJ: Peroxidases. In Biotransformation. Edited by Guengerich FP. Oxford: Elsevier Science Ltd.; 1997:149-163. [Sipes IG, McQueen CA, Gandolfi AJ (Series Editors): Comprehensive Toxicology, vol 3.]
-
(1997)
Biotransformation
, pp. 149-163
-
-
Marnett, L.J.1
-
18
-
-
0030941243
-
Leukocyte 12-lipoxygenase: Expression, purification, and investigation of the role of methionine residues in turnover-dependent inactivation and 5,8,11,14-eicosatetraynoic acid inhibition
-
Richards KM, Marnett LJ: Leukocyte 12-lipoxygenase: expression, purification, and investigation of the role of methionine residues in turnover-dependent inactivation and 5,8,11,14-eicosatetraynoic acid inhibition. Biochemistry 1997, 36:6692-6699.
-
(1997)
Biochemistry
, vol.36
, pp. 6692-6699
-
-
Richards, K.M.1
Marnett, L.J.2
-
19
-
-
0028061322
-
Expression, purification, and characterization of porcine leukocyte 12-lipoxygenase produced in the methyltrophic yeast, Pichia pastoris
-
Reddy RG, Yoshimoto T, Yamamoto S, Marnett LJ: Expression, purification, and characterization of porcine leukocyte 12-lipoxygenase produced in the methyltrophic yeast, Pichia pastoris. Biochem Biophys Res Commun 1994, 205:381-388.
-
(1994)
Biochem Biophys Res Commun
, vol.205
, pp. 381-388
-
-
Reddy, R.G.1
Yoshimoto, T.2
Yamamoto, S.3
Marnett, L.J.4
-
20
-
-
0029114645
-
High-level expression of functional rat neuronal nitric oxide synthase in Escherichia coli
-
Roman U, Sheta EA, Martasek P, Gross SS, Liu Q, Masters BSS: High-level expression of functional rat neuronal nitric oxide synthase in Escherichia coli. Proc Natl Acad Sci USA 1995, 92:8428-8432.
-
(1995)
Proc Natl Acad Sci USA
, vol.92
, pp. 8428-8432
-
-
Roman, U.1
Sheta, E.A.2
Martasek, P.3
Gross, S.S.4
Liu, Q.5
Masters, B.S.S.6
-
21
-
-
0029048347
-
Neuronal nitric oxide synthase: Expression in Escherichia coli, irreversible inhibition by phenyldiazene, and active site topology
-
Gerber NC, Ortiz de Montellano PR: Neuronal nitric oxide synthase: expression in Escherichia coli, irreversible inhibition by phenyldiazene, and active site topology. J Biol Chem 1995, 270:17791-17796.
-
(1995)
J Biol Chem
, vol.270
, pp. 17791-17796
-
-
Gerber, N.C.1
De Ortiz Montellano, P.R.2
-
22
-
-
0029852566
-
New applications of bacterial systems to problems in toxicology
-
Guengerich FP, Gillam EMJ, Shimada T: New applications of bacterial systems to problems in toxicology. Crit Rev Toxicol 1996, 26:551-583. A number of advances in the use of genetically engineered microbes to further the understanding of toxicological problems are highlighted in this review. Salmonella tester strains with different reporter systems such as umu/β-gal and SOS are described. Expression of many drug-metabolizing enzymes including glutathione S-transferase, N-acetyl transferase, ST, cytochrome P450, and P450 reductase in E. coli, and purification and characterization of the recombinant enzymes is discussed in some detail.
-
(1996)
Crit Rev Toxicol
, vol.26
, pp. 551-583
-
-
Guengerich, F.P.1
Gillam, E.M.J.2
Shimada, T.3
-
23
-
-
0029757089
-
Purification of recombinant human cytochrome P450 enzymes expressed in bacteria
-
Guengerich FP, Martin MV, Guo Z, Chun Y-J: Purification of recombinant human cytochrome P450 enzymes expressed in bacteria. Methods Enzymol 1996, 272:35-44. General strategies for the construction, purification, and characterization of recombinant P450s expressed in E. coli and Salmonella are outlined and relative advantages, disadvantages and potential pitfalls are discussed.
-
(1996)
Methods Enzymol
, vol.272
, pp. 35-44
-
-
Guengerich, F.P.1
Martin, M.V.2
Guo, Z.3
Chun, Y.-J.4
-
24
-
-
0022847113
-
Expression of bovine 17 α-hydroxylase cytochrome P-450 cDNA in nonsteroidogenic (COS 1) cells
-
Zuber MX, Simpson ER, Waterman MR: Expression of bovine 17 α-hydroxylase cytochrome P-450 cDNA in nonsteroidogenic (COS 1) cells. Science 1986, 234:1258-1261.
-
(1986)
Science
, vol.234
, pp. 1258-1261
-
-
Zuber, M.X.1
Simpson, E.R.2
Waterman, M.R.3
-
25
-
-
0022610928
-
Monooxygenase activity of Saccharomyces cerevisiae cells transformed with expression plasmids carrying rat cytochrome P-450MC cDNA
-
Sakaki T, Oeda K, Yabusaki Y, Ohkawa H: Monooxygenase activity of Saccharomyces cerevisiae cells transformed with expression plasmids carrying rat cytochrome P-450MC cDNA. J Biochem 1986, 99:741-749.
-
(1986)
J Biochem
, vol.99
, pp. 741-749
-
-
Sakaki, T.1
Oeda, K.2
Yabusaki, Y.3
Ohkawa, H.4
-
26
-
-
0023279799
-
Expression of a functional 78,000 dalton mammalian flavoprotein, NADPH-cytochrome P-450 oxidoreductase, in Escherichia coli
-
Porter TD, Wilson TE, Kasper CB: Expression of a functional 78,000 dalton mammalian flavoprotein, NADPH-cytochrome P-450 oxidoreductase, in Escherichia coli. Arch Biochem Biophys 1987, 254:353-367.
-
(1987)
Arch Biochem Biophys
, vol.254
, pp. 353-367
-
-
Porter, T.D.1
Wilson, T.E.2
Kasper, C.B.3
-
27
-
-
0025819695
-
2-terminal segment retains catalytic activity and is membrane-bound when expressed in Escherichia coli
-
2-terminal segment retains catalytic activity and is membrane-bound when expressed in Escherichia coli. J Biol Chem 1991, 266:7321-7324.
-
(1991)
J Biol Chem
, vol.266
, pp. 7321-7324
-
-
Larson, J.R.1
Coon, M.J.2
Porter, T.D.3
-
28
-
-
0025994649
-
Expression and enzymatic activity of recombinant cytochrome P450 17 α-hydroxylase in Escherichia coli
-
Barnes HJ, Arlotto MP, Waterman MR: Expression and enzymatic activity of recombinant cytochrome P450 17 α-hydroxylase in Escherichia coli. Proc Natl Acad Sci USA 1991, 88:5597-5601.
-
(1991)
Proc Natl Acad Sci USA
, vol.88
, pp. 5597-5601
-
-
Barnes, H.J.1
Arlotto, M.P.2
Waterman, M.R.3
-
29
-
-
0029971798
-
Multiple forms of human P450 expressed in Saccharomyces cerevisiae: Systematic characterization and comparison with those of the rat
-
Imaoka S, Yamada T, Hiroi T, Hayashi K, Sakaki T, Yabusaki Y, Funae Y: Multiple forms of human P450 expressed in Saccharomyces cerevisiae: systematic characterization and comparison with those of the rat Biochem Pharmacol 1996, 51:1041-1050. Nine human microsomal P450 enzymes were expressed and purified from S. cerevisiae. A number of rat P450s were purified from rat liver microsomes and enzymatic activities were systematically compared.
-
(1996)
Biochem Pharmacol
, vol.51
, pp. 1041-1050
-
-
Imaoka, S.1
Yamada, T.2
Hiroi, T.3
Hayashi, K.4
Sakaki, T.5
Yabusaki, Y.6
Funae, Y.7
-
30
-
-
0028298478
-
Generation of the soluble and functional cytosolic domain of microsomal cytochrome P450 52A3
-
Scheller U, Kraft R, Schösder KL, Schunck WH: Generation of the soluble and functional cytosolic domain of microsomal cytochrome P450 52A3. J Biol Chem 1994, 269:12779-12783.
-
(1994)
J Biol Chem
, vol.269
, pp. 12779-12783
-
-
Scheller, U.1
Kraft, R.2
Schösder, K.L.3
Schunck, W.H.4
-
31
-
-
0030021995
-
Recombinant human cytochrome P450 1A2 and an N-terminal truncated form: Construction, purification, aggregation properties, and interactions with flavodoxin, ferredoxin, and NADPH-cytochrome P450 reductase
-
Dong M-S, Yamazaki H, Guo Z, Guengerich FP: Recombinant human cytochrome P450 1A2 and an N-terminal truncated form: construction, purification, aggregation properties, and interactions with flavodoxin, ferredoxin, and NADPH-cytochrome P450 reductase. Arch Biochem Biophys 1996, 327:11-19.
-
(1996)
Arch Biochem Biophys
, vol.327
, pp. 11-19
-
-
Dong, M.-S.1
Yamazaki, H.2
Guo, Z.3
Guengerich, F.P.4
-
32
-
-
0029142916
-
Molecular engineering of microsomal P450 2a-4 to a stable, water-soluble enzyme
-
Sueyoshi T, Park U, Moore R, Juvonen RO, Negishi M: Molecular engineering of microsomal P450 2a-4 to a stable, water-soluble enzyme. Arch Biochem Biophys 1995, 322:265-271.
-
(1995)
Arch Biochem Biophys
, vol.322
, pp. 265-271
-
-
Sueyoshi, T.1
Park, U.2
Moore, R.3
Juvonen, R.O.4
Negishi, M.5
-
34
-
-
0028977906
-
Expression of cytochrome P450 2D6 in Escherichia coli, purification, and spectral and catalytic characterization
-
Gillam EMJ, Guo Z, Martin MV, Jenkins CM, Guengerich FP: Expression of cytochrome P450 2D6 in Escherichia coli, purification, and spectral and catalytic characterization. Arch Biochem Biophys 1995, 319:540-550.
-
(1995)
Arch Biochem Biophys
, vol.319
, pp. 540-550
-
-
Gillam, E.M.J.1
Guo, Z.2
Martin, M.V.3
Jenkins, C.M.4
Guengerich, F.P.5
-
35
-
-
0031106366
-
Microsomal P450 2C3 is expressed as a soluble dimer in Escherichia coli following modifications of its N-terminus
-
Von Wachenfeldt C, Richardson TH, Cosme J, Johnson EF: Microsomal P450 2C3 is expressed as a soluble dimer in Escherichia coli following modifications of its N-terminus. Arch Biochem Biophys 1997, 339:107-114. Modifications of the amino terminus of rabbit microsomal P450 2C3 permitted high-level expression of the enzyme. Deletion of residues 3-20, the putative membrane-spanning anchor sequence, rendered the protein more soluble, particularly in the presence of extraction buffers containing high concentrations of salt. Addition of a histidine tag to the protein facilitated purification by nickel chromatography in the absence of detergent.
-
(1997)
Arch Biochem Biophys
, vol.339
, pp. 107-114
-
-
Von Wachenfeldt, C.1
Richardson, T.H.2
Cosme, J.3
Johnson, E.F.4
-
36
-
-
0029763107
-
Identification of retained N-formylmethionine in bacterial recombinant cytochrome P450 proteins with the N-terminal sequence MALLLAVFL...: Roles of residues 3-5 in retention and membrane topology
-
Dong M-S, Bell LC, Guo Z, Phillips DR, Blair IA, Guengerich FP: Identification of retained N-formylmethionine in bacterial recombinant cytochrome P450 proteins with the N-terminal sequence MALLLAVFL...: roles of residues 3-5 in retention and membrane topology. Biochemistry 1996, 35:10031-10040. Random mutagenesis of residues 3-5 of human cytochrome P450 1A2 was effected in order to determine the cause of an observed block to Edman degradation of recombinant P450s containing the amino-terminal sequence MALLLAVFL... of several P450s expressed in bacteria. It was determined that retention of amino formylmethionine causes the block and that the N-terminus either interacts with the membranes or is free in the cytosol, depending on the sequence composition of this region.
-
(1996)
Biochemistry
, vol.35
, pp. 10031-10040
-
-
Dong, M.-S.1
Bell, L.C.2
Guo, Z.3
Phillips, D.R.4
Blair, I.A.5
Guengerich, F.P.6
-
37
-
-
0029884802
-
Construction of a human cytochrome P450 1A1:rat NADPH-P450 reductase fusion protein cDNA, expression in Escherichia coli, purification, and catalytic properties of the enzyme in bacterial cells and after purification
-
Chun Y-J, Shimada T, Guengerich FP: Construction of a human cytochrome P450 1A1:rat NADPH-P450 reductase fusion protein cDNA, expression in Escherichia coli, purification, and catalytic properties of the enzyme in bacterial cells and after purification. Arch Biochem Biophys 1996, 330:48-58.
-
(1996)
Arch Biochem Biophys
, vol.330
, pp. 48-58
-
-
Chun, Y.-J.1
Shimada, T.2
Guengerich, F.P.3
-
38
-
-
0031127850
-
Expression, purification, and characterization of a catalytically active human cytochrome P450 1A2:NADPH-cytochrome P450 reductase fusion protein
-
Parikh A, Guengerich FP: Expression, purification, and characterization of a catalytically active human cytochrome P450 1A2:NADPH-cytochrome P450 reductase fusion protein. Protein Expr Purif 1997, 9:346-354.
-
(1997)
Protein Expr Purif
, vol.9
, pp. 346-354
-
-
Parikh, A.1
Guengerich, F.P.2
-
39
-
-
0029859961
-
Molecular engineering study on electron transfer from NADPH-P450 reductase to rat mitochondrial P450c27 in yeast microsomes
-
Sakaki T, Kominami S, Hayashi K, Akiyoshi-Shibata M, Yabusaki Y: Molecular engineering study on electron transfer from NADPH-P450 reductase to rat mitochondrial P450c27 in yeast microsomes. J Biol Chem 1996, 271:26209-26213. This group demonstrated previously that exchanging the bovine microsomal P450 17A optimized amino-terminal leader sequence for the rat mitochondrial P450c27 amino-terminal sequence was sufficient to redirect localization of the mitochondrial enzyme to microsomes in a yeast expression system. This study shows that in the absence of the mitochondrial electron transport chain (adrenodoxin reductase, adrenodoxin) the modified P450c27 accepts electrons from NADPH via the yeast NADPH-P450 reductase. Construction of a fusion protein between the rat P450 and yeast reductase yielded a protein in which the anaerobic reduction rate of the heme was reported to occur so quickly it could not be measured in a stopped-flow spectrophotometer, although the data were not presented.
-
(1996)
J Biol Chem
, vol.271
, pp. 26209-26213
-
-
Sakaki, T.1
Kominami, S.2
Hayashi, K.3
Akiyoshi-Shibata, M.4
Yabusaki, Y.5
-
40
-
-
0030249221
-
The interaction of NADPH-P450 reductase with P450: An electrochemical study of the role of the flavin mononucleotide-binding domain
-
Estabrook RW, Shet MS, Fisher CW, Jenkins CM, Waterman MR: The interaction of NADPH-P450 reductase with P450: an electrochemical study of the role of the flavin mononucleotide-binding domain. Arch Biochem Biophys 1996, 333:308-315.
-
(1996)
Arch Biochem Biophys
, vol.333
, pp. 308-315
-
-
Estabrook, R.W.1
Shet, M.S.2
Fisher, C.W.3
Jenkins, C.M.4
Waterman, M.R.5
-
41
-
-
0029809775
-
cam triple fusion protein. Construction of a self-sufficient Escherichia coli catalytic system
-
cam triple fusion protein. Construction of a self-sufficient Escherichia coli catalytic system. J Biol Chem 1996, 271:22462-22469. The bacterial cytochrome P450 101 electron transport chain was expressed as three covalently linked components of a fusion protein in varying orders and with varying linkers. The most active construct was one in which putidaredoxin reductase, putidaredoxin, and P450 101 were arranged in that order. The rate-limiting step in enzymatic catalysis was determined to involve electron transfer from putidaredoxin to P450. Additionally, this was the first demonstration of a catalytically self-sufficient system for oxidation by P450 101.
-
(1996)
J Biol Chem
, vol.271
, pp. 22462-22469
-
-
Sibbesen, O.1
De Voss, J.J.2
De Ortiz Montellano, P.R.3
-
42
-
-
0029012009
-
CYP3A4 expressed by insect cells infected with a recombinant baculovirus containing both CYP3A4 and human NADPH-cytochrome P450 reductase is catalytically similar to human liver microsomal CYP3A4
-
Lee CA, Kadwell SH, Kost TA, Serabjit-Singh CJ: CYP3A4 expressed by insect cells infected with a recombinant baculovirus containing both CYP3A4 and human NADPH-cytochrome P450 reductase is catalytically similar to human liver microsomal CYP3A4. Arch Biochem Biophys 1995, 319:157-167.
-
(1995)
Arch Biochem Biophys
, vol.319
, pp. 157-167
-
-
Lee, C.A.1
Kadwell, S.H.2
Kost, T.A.3
Serabjit-Singh, C.J.4
-
43
-
-
0029986770
-
Functional high level expression of cytochrome P450 CYP2D6 using baculoviral expression systems
-
Paine MJI, Gilham D, Roberts GCK, Wolf CR: Functional high level expression of cytochrome P450 CYP2D6 using baculoviral expression systems. Arch Biochem Biophys 1996, 328:143-150.
-
(1996)
Arch Biochem Biophys
, vol.328
, pp. 143-150
-
-
Paine, M.J.I.1
Gilham, D.2
Roberts, G.C.K.3
Wolf, C.R.4
-
44
-
-
0030588264
-
Expression and coupling of human cytochrome P450 2E1 and NADPH-cytochrome P450 oxidoreductase in dual expression and co-infection systems with baculovirus in insect cells
-
Wang M-H, Patten CJ, Yang G-Y, Paranawithana SR, Tan Y, Yang CS: Expression and coupling of human cytochrome P450 2E1 and NADPH-cytochrome P450 oxidoreductase in dual expression and co-infection systems with baculovirus in insect cells. Arch Biochem Biophys 1996, 334:380-388.
-
(1996)
Arch Biochem Biophys
, vol.334
, pp. 380-388
-
-
Wang, M.-H.1
Patten, C.J.2
Yang, G.-Y.3
Paranawithana, S.R.4
Tan, Y.5
Yang, C.S.6
-
45
-
-
0030586725
-
Stable expression of human cytochrome P450 3A4 in conjunction with human NADPH-cytochrome P450 oxidoreductase in V79 Chinese hamster cells
-
Schneider A, Schmalix WA, Siruguri V, de Groene EM, Horbach GJ, Kleingeist B, Lang D, Böcker R, Belloc C, Beaune P et al.: Stable expression of human cytochrome P450 3A4 in conjunction with human NADPH-cytochrome P450 oxidoreductase in V79 Chinese hamster cells. Arch Biochem Biophys 1996, 332:295-304.
-
(1996)
Arch Biochem Biophys
, vol.332
, pp. 295-304
-
-
Schneider, A.1
Schmalix, W.A.2
Siruguri, V.3
De Groene, E.M.4
Horbach, G.J.5
Kleingeist, B.6
Lang, D.7
Böcker, R.8
Belloc, C.9
Beaune, P.10
-
46
-
-
0030592712
-
Coexpression of a human P450 (CYP3A4) and P450 reductase generates a highly functional monooxygenase system in Escherichia coli
-
Blake JAR, Pritchard M, Ding S, Smith GCM, Burchell B, Wolf CR, Friedberg T: Coexpression of a human P450 (CYP3A4) and P450 reductase generates a highly functional monooxygenase system in Escherichia coli. FEBS Lett 1996, 397:210-214. A two-promoter plasmid was constructed for the co-expression of P450 3A4 with human NADPH-P450 reductase in E. coli. Proteins were produced in a -3:1 ratio of NADPH-P450 reductase to P450. Whole bacterial cells as well as isolated bacterial membranes demostrated a high degree of catalytic efficiency as judged by metabolism of testosterone, nifedipine, and erythromycin. This was the first published demonstration of a functional monooxygenase complex in intact E. coli.
-
(1996)
FEBS Lett
, vol.397
, pp. 210-214
-
-
Blake, J.A.R.1
Pritchard, M.2
Ding, S.3
Smith, G.C.M.4
Burchell, B.5
Wolf, C.R.6
Friedberg, T.7
-
47
-
-
0031106383
-
The function of recombinant cytochrome P450s in intact Escherichia coli cells: The I7α-hydroxylation of progesterone and pregnenolone by P450c17
-
Shet MS, Fisher CW, Estabrook RW: The function of recombinant cytochrome P450s in intact Escherichia coli cells: the I7α-hydroxylation of progesterone and pregnenolone by P450c17. Arch Biochem Biophys 1997, 339:218-225. Coexpression of P450 17A with NADPH-P450 reductase showed high rates of progesterone and pregnenolone metabolism in intact E. coli in comparison with a fusion protein containing domains of these two enzymes. It is suggested that the constitutive respiratory activity of E. coli is the major limiting factor in bacterial catalysis of P450-mediated reactions.
-
(1997)
Arch Biochem Biophys
, vol.339
, pp. 218-225
-
-
Shet, M.S.1
Fisher, C.W.2
Estabrook, R.W.3
-
48
-
-
0342682875
-
Catalysis of reactions important in drug metabolism by Escherichia coli expressing human cytochromes P450
-
in press
-
Parikh A, Gillam EMJ, Guengerich FP: Catalysis of reactions important in drug metabolism by Escherichia coli expressing human cytochromes P450. Nat Biotechnol 1997, in press. Coexpression of six major human microsomal P450s with Fp in E. coli demonstrated catalytic efficiency in whole bacterial cells that compared favorably with rates previously observed in mammalian microsomes and reconstituted systems consisting of purified proteins, lipids, and cofactors.
-
(1997)
Nat Biotechnol
-
-
Parikh, A.1
Gillam, E.M.J.2
Guengerich, F.P.3
-
49
-
-
0029838187
-
Expression and characterization of a flavin-containing monooxygenase 4 from humans
-
Itagaki K, Carver GT, Philpot RM: Expression and characterization of a flavin-containing monooxygenase 4 from humans. J Biol Chem 1996, 271:20102-20107. Human microsomal flavin-containing monooxygenase-4 (FMO-4) has long been resistant to heterologous expression. This isoform, when aligned with FMOs 1,2,3, and 5, yields a transcript elongated past the consensus stop site by 80 nucleotides. This report demonstrated that a carboxy-terminally truncated form of FMO-4 (at the consensus stop) can be expressed to high levels in E. coli and displays enzymatic properties consistent with the purified enzyme from porcine liver.
-
(1996)
J Biol Chem
, vol.271
, pp. 20102-20107
-
-
Itagaki, K.1
Carver, G.T.2
Philpot, R.M.3
-
50
-
-
0030998448
-
Baculovirus-directed expression of rabbit UDP-glucuronosyltransferases in Spodoptera frugiperda cells
-
in press
-
Nguyen N, Tukey RH: Baculovirus-directed expression of rabbit UDP-glucuronosyltransferases in Spodoptera frugiperda cells. Drug Metab Dispos 1997, 25:in press. Uridine diphosphate glucuronosyl transferases (UGT) 2B13 and 1A6 were cloned into baculoviral expression vectors and expressed in Sf9 cells. Since glycosylation obviates the possibility of UGT expression in E. coli, direct comparisons made with COS cells suggest that insect cells might potentially replace mammalian cells as an efficient means of obtaining sufficient amounts of catalytically active UGT protein for mechanistic studies.
-
(1997)
Drug Metab Dispos
, pp. 25
-
-
Nguyen, N.1
Tukey, R.H.2
-
52
-
-
0027456534
-
Interaction of hepatic microsomal epoxide hydrolase derived from a recombinant baculovirus expression system with an azarene oxide and an aziridine substrate analogue
-
Lacourciere GM, Vakharia VN, Tan CP, Morris Dl, Edwards GH, Moos M, Armstrong RN: Interaction of hepatic microsomal epoxide hydrolase derived from a recombinant baculovirus expression system with an azarene oxide and an aziridine substrate analogue. Biochemistry 1993, 32:2610-2616.
-
(1993)
Biochemistry
, vol.32
, pp. 2610-2616
-
-
Lacourciere, G.M.1
Vakharia, V.N.2
Tan, C.P.3
Dl, M.4
Edwards, G.H.5
Moos, M.6
Armstrong, R.N.7
-
53
-
-
0027267290
-
Expression of rat microsomal epoxide hydrolase in Escherichia coli: Identification of a histidyl residue essential for catalysis
-
Bell PA, Kasper CB: Expression of rat microsomal epoxide hydrolase in Escherichia coli: identification of a histidyl residue essential for catalysis. J Biol Chem 1993, 268:14011-14017.
-
(1993)
J Biol Chem
, vol.268
, pp. 14011-14017
-
-
Bell, P.A.1
Kasper, C.B.2
-
54
-
-
0027929981
-
The transmembrane region of microsomal cytochrome P450 identified as the endoplasmic reticulum retention signal
-
Murakami K, Mihara K, Omura T: The transmembrane region of microsomal cytochrome P450 identified as the endoplasmic reticulum retention signal. J Biochem 1994, 116:164-175.
-
(1994)
J Biochem
, vol.116
, pp. 164-175
-
-
Murakami, K.1
Mihara, K.2
Omura, T.3
-
55
-
-
0028885696
-
The cytoplasmic and N-terminal transmembrane domains of cytochrome P450 contain independent signals for retention in the endoplasmic reticulum
-
Szczesna-Skorupa E, Ahn K, Chen CD, Doray B, Kemper B: The cytoplasmic and N-terminal transmembrane domains of cytochrome P450 contain independent signals for retention in the endoplasmic reticulum. J Biol Chem 1995, 270:24327-24333.
-
(1995)
J Biol Chem
, vol.270
, pp. 24327-24333
-
-
Szczesna-Skorupa, E.1
Ahn, K.2
Chen, C.D.3
Doray, B.4
Kemper, B.5
-
56
-
-
0029970611
-
Establishment of transgenic mice carrying human fetus-specific CYP3A7
-
1, proving overexpression of the transgene. Background from mouse 3A enzymes, however, complicated the analysis of 3A7 function and results should be interpreted with some caution.
-
(1996)
Arch Biochem Biophys
, vol.329
, pp. 235-240
-
-
Li, Y.1
Yokoi, T.2
Kitamura, R.3
Sasaki, M.4
Gunji, M.5
Katsuki, M.6
Kamataki, T.7
-
57
-
-
0002888510
-
Human cytochrome P450 enzymes
-
Edited by Ortiz de Montellano PR. New York: Plenum Press
-
Guengerich FP: Human cytochrome P450 enzymes. In Cytochrome P450. Edited by Ortiz de Montellano PR. New York: Plenum Press; 1995:473-535.
-
(1995)
Cytochrome P450
, pp. 473-535
-
-
Guengerich, F.P.1
-
58
-
-
0030588661
-
Recombinant DNA technology as an investigative tool in drug metabolism research
-
Friedberg T, Wolf CR: Recombinant DNA technology as an investigative tool in drug metabolism research. Adv Drug Deliv Rev 1996, 22:187-213. Review emphasizing the major advances in molecular biological techniques that have permitted investigation of the enzymatic properties and regulation of a number of drug- and xenobiotic-metabolizing enzymes.
-
(1996)
Adv Drug Deliv Rev
, vol.22
, pp. 187-213
-
-
Friedberg, T.1
Wolf, C.R.2
-
59
-
-
0029925607
-
Use of terfenadine and contraindicated drugs
-
Thompson D, Oster G: Use of terfenadine and contraindicated drugs. JAMA 1996, 275:1339-1341.
-
(1996)
JAMA
, vol.275
, pp. 1339-1341
-
-
Thompson, D.1
Oster, G.2
-
60
-
-
0000179727
-
Carcinogens are mutagens: A simple test system combining liver homogenates for activation and bacteria for detection
-
Ames BN, Durston WE, Yamasaki E, Lee FD: Carcinogens are mutagens: a simple test system combining liver homogenates for activation and bacteria for detection. Proc Natl Acad Sci USA 1973, 70:2281-2285.
-
(1973)
Proc Natl Acad Sci USA
, vol.70
, pp. 2281-2285
-
-
Ames, B.N.1
Durston, W.E.2
Yamasaki, E.3
Lee, F.D.4
-
61
-
-
0029947171
-
Human glutathione S-transferase T1-1 enhances mutagenicity of 1,2-dibromoethane, dibromomethane, and 1,2,3,4-diepoxybutane in Salmonella typhimurium
-
Thier R, Pemble S, Kramer H, Taylor JB, Guengerich FP, Ketterer B: Human glutathione S-transferase T1-1 enhances mutagenicity of 1,2-dibromoethane, dibromomethane, and 1,2,3,4-diepoxybutane in Salmonella typhimurium. Carcinogenesis 1996, 17:163-166.
-
(1996)
Carcinogenesis
, vol.17
, pp. 163-166
-
-
Thier, R.1
Pemble, S.2
Kramer, H.3
Taylor, J.B.4
Guengerich, F.P.5
Ketterer, B.6
-
62
-
-
0030070661
-
Activation and inactivation of carcinogenic dihaloalkanes and other compounds by glutathione S-transferase 5-5 in Salmonella typhimurium tester strain NM5004
-
Shimada T, Yamazaki H, Oda Y, Hiratsuka A, Watabe T, Guengerich FP: Activation and inactivation of carcinogenic dihaloalkanes and other compounds by glutathione S-transferase 5-5 in Salmonella typhimurium tester strain NM5004. Chem Res Toxicol 1996, 9:333-340.
-
(1996)
Chem Res Toxicol
, vol.9
, pp. 333-340
-
-
Shimada, T.1
Yamazaki, H.2
Oda, Y.3
Hiratsuka, A.4
Watabe, T.5
Guengerich, F.P.6
-
63
-
-
0029925074
-
A new Salmonella typhimurium NM5004 strain expressing rat glutathione S-transferase 5-5: Use in detection of genotoxicity of dihaloalkanes using an SOS/umu test system
-
Oda Y, Yamazaki H, Thier R, Ketterer B, Guengerich FP, Shimada T: A new Salmonella typhimurium NM5004 strain expressing rat glutathione S-transferase 5-5: use in detection of genotoxicity of dihaloalkanes using an SOS/umu test system. Carcinogenesis 1996, 17:297-302.
-
(1996)
Carcinogenesis
, vol.17
, pp. 297-302
-
-
Oda, Y.1
Yamazaki, H.2
Thier, R.3
Ketterer, B.4
Guengerich, F.P.5
Shimada, T.6
-
64
-
-
0030008731
-
Activation of chemically diverse procarcinogens by human cytochrome P450 1B1
-
Shimada T, Hayes CL, Yamazaki H, Amin S, Hecht SS, Guengerich FP, Sutter TR: Activation of chemically diverse procarcinogens by human cytochrome P450 1B1. Cancer Res 1996, 56:2979-2984.
-
(1996)
Cancer Res
, vol.56
, pp. 2979-2984
-
-
Shimada, T.1
Hayes, C.L.2
Yamazaki, H.3
Amin, S.4
Hecht, S.S.5
Guengerich, F.P.6
Sutter, T.R.7
-
65
-
-
19244383028
-
Development of an in situ toxicity assay system using recombinant baculoviruses
-
Grant DF, Greene JF, Pinot F, Borhan B, Moghaddam MF, Hammock BD, McCutchen B, Ohkawa H, Juo G, Guenthner TM: Development of an in situ toxicity assay system using recombinant baculoviruses. Biochem Pharmacol 1996, 51:503-515. The capacity of various drugs and xenobiotics to prevent or enhance cellular toxicity was measured in situ using Sf21 insect cells infected with recombinant baculoviruses. Initial studies were perfomed using recombinant mouse soluble epoxide hydrolase and rat P450 1A1. A general strategy for determining toxicities in cell culture is described.
-
(1996)
Biochem Pharmacol
, vol.51
, pp. 503-515
-
-
Grant, D.F.1
Greene, J.F.2
Pinot, F.3
Borhan, B.4
Moghaddam, M.F.5
Hammock, B.D.6
McCutchen, B.7
Ohkawa, H.8
Juo, G.9
Guenthner, T.M.10
-
66
-
-
0028116034
-
Metabolism of chlorofluorocarbons and polybrominated compounds by Pseudomonas putida G786(pHG-2) via an engineered metabolic pathway
-
Hur HG, Sadowsky MJ, Wackett LP: Metabolism of chlorofluorocarbons and polybrominated compounds by Pseudomonas putida G786(pHG-2) via an engineered metabolic pathway. Appl Environ Microbiol 1994, 60:4148-4154.
-
(1994)
Appl Environ Microbiol
, vol.60
, pp. 4148-4154
-
-
Hur, H.G.1
Sadowsky, M.J.2
Wackett, L.P.3
|