Glubodies: Randomized libraries of glutathione transferase enzymes

Chemistry and Biology

Volumn 3, Issue 5, 1996, Pages 359-367

  Napolitano, Eugene W ^a   Villar, Hugo O ^a   Kauvar, Lawrence M ^a   Higgins, Deborah L ^a   Roberts, Doug ^a   Mandac, Janis ^a   Lee, Sandra K ^a   Engqvist Goldstein, Asa ^a   Bukar, Robert ^a   Calio, Brenda L ^a   Jäck, Hans Martin ^a,b   Tainer, John A ^a,c  

^a Terrapin Technologies Inc   (United States)

^b LOYOLA UNIVERSITY CHICAGO   (United States)

^c SCRIPPS RESEARCH INSTITUTE   (United States)

Author keywords

Glutathione S transferase (GST); Ligand recognition segment (LRS); Randomized libraries; Substrate specificity

Indexed keywords

GLUTATHIONE TRANSFERASE; PRIMER DNA;

AMINO ACID SEQUENCE; ARTICLE; CHEMISTRY; ENZYME SPECIFICITY; GENETICS; HUMAN; METABOLISM; MOLECULAR GENETICS; MUTAGENESIS; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PROTEIN CONFORMATION;

AMINO ACID SEQUENCE; BASE SEQUENCE; DNA PRIMERS; GLUTATHIONE TRANSFERASE; HUMANS; MOLECULAR SEQUENCE DATA; MUTAGENESIS; POLYMERASE CHAIN REACTION; PROTEIN CONFORMATION; SUBSTRATE SPECIFICITY;

EID: 0030133645     PISSN: 10745521     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1074-5521(96)90119-2     Document Type: Article

References (31)

1. Tainer, J.A., Deal, C.D., Geysen, H.M., Roberts, V.A. & Getzoff, E.D. (1991). Defining antibody-antigen recognition: towards engineered antibodies and epitopes. Int. Rev. Immunol. 7, 165-188.
2. Edmundson, A.B., Guggat, L.W., Shan, L., Fan, Z.C. & Hanson, B.L. (1994). Structural aspects of conformational changes in ligand binding by antibody fragments. Res. Immunol. 145, 56-61.
3. Marks, J.D., Hoogenboom, H.R., Bonnert, T.P., McCafferty, J., Griffiths, A.D. & Winter, G. (1991). By-passing immunization: human antibodies from V-gene libraries displayed on phage. J. Mol. Biol. 222, 581-597.
4. Vanderlaan, M., Stanker, L.H., Watkins, B.E. & Roberts, D.W., Eds. (1991). Immunoanalysis for Trace Chemical Analysis. Washington, D.C., ACS Symposium Series #451.
5. Lerner, R.A., Benkovic, S.J. & Schultz, P.G. (1991). At the crossroads of chemistry and immunology: catalytic antibodies. Science 252, 659-667.
6. Jakoby, W.B. & Ziegler, D.M. (1990). The enzymes of detoxification. J. Biol. Chem. 265, 20175-20178.
7. Mannervik, B. & Danielson, U.H. (1988). Glutathione transferases: structure and catalytic activity. CRC Crit. Rev. Biochem. 23, 283-337.
8. Ricci, G., et al., & Federici, G. (1995). Site-directed mutagenesis of human glutathione transferase P1-1. J. Biol. Chem. 270, 1243-1248.
9. Palzkill, T. & Botstein, D. (1992). Probing β-lactamase structure and function using random replacement mutagenesis. Proteins 14, 29-44.
10. Munir, K.M., French, D.C., Dube, D.K. & Loeb, L.A. (1994). Herpes thymidine kinase mutants with altered catalytic efficiencies obtained by random sequence selection. Protein Eng. 7, 83-89.
11. Black, M.E. & Loeb, L.A. (1993) Identification of important residues within the putative nucleoside binding site of HSV-1 thymidine kinase by random sequence selection: analysis of selected mutants in vitro. Biochemistry 32, 11618-11626.
12. Gulick. A.M. & Fahl, W.E. (1995). Forced evolution of glutathione S-transferase to create a more efficient drug detoxification enzyme. Proc Natl Acad Sci USA 92, 8140-8144.
13. Widersten, M. & Mannervik, B. (1995). Glutathione transferases with novel active sites isolated by phage display from a library of random mutants. J. Mol. Biol. 250, 115-122.
14. Mannervik, B., Guthenberg, C. Jakobsen, I. & Warholm, M. (1978). Glutathione conjugation: reaction mechanism of glutathione S-transferase. In Conjugation Reactions in Drug Biotransformation. (Aito, A., ed.), pp. 101-122, Elsevier/North Holland Biomedical Press, Amsterdam.
15. Kano, T., Sakai, N. & Muramatsu, M. (1987). Structure and expression of a human class Pi glutathione S-transferase messenger RNA. Cancer Res. 47, 5626-5630.
16. Reinemer, P., et. al., & Parker, M.W. (1992). Three dimensional structure of class Pi glutathione-S-transferase from human placenta in complex with S-hexylglutathione at 2.8 A resolution. J. Mol. Biol. 227, 214-226.
17. Chothia, C. & Lesk, A.M. (1987). Canonical structures for the hypervariable regions of immunoglobulins. J. Mol. Biol. 196, 901-917.
18. Ji, X., Zhang, P., Armstrong, R.N. & Gilliland, G.L. (1992). The three dimensional structure of a glutathione S-transferase from the Mu-gene class. Structural analysis of the binary complex of isozyme 3-3 and glutathione at 2.2 Å resolution. Biochemistry 31, 10169-10184.
19. Cachau, R.E., Erickson, J.W. & Villar, H.O. (1994). Novel procedure for structure refinement in homology modeling and its application to the human class Mu glutathione S-transferases. Protein Eng. 7, 831-839.
20. Kolm, R.H., Sroga, G.E. & Mannervik, B. (1992). Participation of the phenolic hydroxyl group of Try-8 in the catalytic mechanism of human glutathione transferase P1-1. Biochem. J. 285, 537-540.
21. Hoogenboom, H.R., Griffiths, A.D., Johnson, K.S., Chiswell, D.J., Hudson, P. & Winter, G. (1991). Multi-subunit proteins on the surface of filamentous phage: methodologies for displaying antibody (Fab) heavy and light chains. Nucleic Acids Res. 19, 4133-4137.
22. Wheatley, J.B., Hughes, B., Bauer, K. & Schmidt, D.E. (1994). Study of chromatographic parameters for glutathione S-transferases on high-performance liquid chromatography affinity stationary phase. J. Chromatography 676, 81-90.
23. Flatgaard, J.E., Bauer, K.E. & Kauvar, L.M. (1993). Isozyme specificity of novel glutathione S-transferase inhibitors. Cancer Chemother. Pharmacol. 33, 63-70.
24. Kauvar, L.M., et. al., & Rocke, D.M. (1995). Predicting ligand binding to proteins by affinity fingerprinting. Chemistry & Biology 2, 107-118.
25. Ku, J. & Schultz, P.G. (1995). Alternate protein frameworks for molecular recognition. Proc. Natl. Acad. Sci. USA 92, 6552-6556.
26. Lyttle, M.H., Napolitano, E.W., Calio, B.L. & Kauvar, L.M. (1995). Mutagenensis using trinucleotide β-cyanoethyl phosphoramidites. Biotechniques 19, 274-281.
27. Kauvar, L.M. (1994). Panels of analyte binding ligands. U.S. Patent #5,340,474.
28. Roberts, V.A., et. al., & Tainer, J.A. (1990). Antibody remodeling: a general solution to the design of a metal-coordination site in an antibody binding pocket. Proc Natl Acad Sci USA 87, 6654-6658.
29. Kolm, R.H., Stenberg, G., Widersten, M. & Mannervik, B. (1995). High-level bacterial expression of human glutathione transferase P1-1 encoded by semisynthetic DNA. Protein Expr. Purif. 6, 265-271.
30. Stenberg, G. (1991). Glutathione transferases: molecular cloning, site-directed mutagenesis and structure-function studies. Ph.D. thesis, University of Stockholm.
31. Barbas, C.F., Bain, J.D., Hoekstra, D.M. & Lerner, R.A. (1992). Semisynthetic combinatorial antibody libraries: a chemical solution to the diversity problem. Proc. Natl. Acad. Sci. USA 89, 4457-4461.