Optimized heterologous expression of the polymorphic human glutathione transferase M1-1 based on silent mutations in the corresponding cDNA

Protein Expression and Purification

Volumn 7, Issue 4, 1996, Pages 367-372

  Widersten, Mikael ^a   Huang, Mao ^a   Mannervik, Bengt ^a  

^a UPPSALA UNIVERSITY   (Sweden)

Author keywords

[No Author keywords available]

Indexed keywords

ANIMALIA; ESCHERICHIA COLI; ORYCTOLAGUS CUNICULUS;

EID: 0030175041     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1006/prep.1996.0054     Document Type: Article

References (25)

1. 1. Kolm, R. H., Stenberg, G., Widersten, M., and Mannervik, B. (1995) High-level bacterial expression of human glutathione transferase P1-1 encoded by semi-synthetic DNA. Protein Expression Purif. 6, 265-271.
2. 2. Andersson, S. G. E., and Kurland, C. G. (1990) Codon preferences in free-living microorganisms. Microbiol. Rev. 54, 198-210.
3. 3. Björnestedt, R., Tardioli, S., and Mannervik, B. (1995) The high activity of rat glutathione transferase 8-8 with alkene substrates is dependent on a glycine residue in the active site. J. Biol. Chem. 270, 29705-29709.
4. 4. Bücheler, U. S., Werner, D., and Schirmer, R. H. (1990) Random silent mutagenesis in the initial triplets of the coding region: A technique for adapting human glutathione reductase-encoding cDNA to expression in Escherichia coli. Gene 96, 271-276.
5. 5. Jiang, F., Hellman, U., Sroga, G. E., Bergman, B., and Mannervik, B. (1995) Cloning, sequencing, and regulation of the glutathione reductase gene from the cyanobacterium Anabaena PCC 7120. J. Biol. Chem. 270, 22882-22889.
6. 6. Ridderström, M., and Mannervik, B. (1996) Optimized heterologous expression of the human zinc enzyme glyoxalase I. Biochem. J. 314, 463-467.
7. 7. Ridderström, M., Saccucci, F., Hellman, U., Bergman, T., Principato, G., and Mannervik, B. (1996) Molecular cloning, heterologous expression, and characterization of human glyoxalase II. J. Biol. Chem. 271, 319-323.
8. 8. Mannervik, B., Awasthi, Y. C., Board, P. G., Hayes, J. D., Di Ilio, C., Ketterer, B., Listowsky, I., Morgenstern, R., Muramatsu, M., Pearson, W. R., Pickett, C. B., Sato, K., Widersten, M., and Wolf, C. R. (1992) Nomenclature for human glutathione transferases. Biochem. J. 282, 305-306.
9. 9. Widersten, M., Pearson, W. R., Engström, Å., and Mannervik, B. (1991) Heterologous expression of the allelic variant Mu-class glutathione transferases μ and ψ. Biochem J. 276, 519-524.
10. 10. Warholm, M., Guthenberg, C., and Mannervik, B. (1983) Molecular and catalytic properties of glutathione transferase μ from human liver: An enzyme efficiently conjugating epoxides. Biochemistry 22, 3610-3617.
11. 11. Warholm, M., Guthenberg, C., Mannervik, B., and von Bahr, C. (1981) Purification of a new glutathione S-transferase (transferase μ) from human liver having high activity with benzo(α)-pyrene-4,5-oxide. Biochem. Biophys. Res. Commun. 98, 512-519.
12. 12. Seidegård, J., DePierre, J. W., and Pero, R. W. (1985) Hereditary individual differences in the glutathione transferase activity towards trans-stilbene oxide in resting human mononuclear leukocytes are due to a particular isozyme(s). Carcinogenesis 6, 1211-1216.
13. 13. Seidegård, J., Pero, R. W., Miller, D. G., and Beattie, E. J. (1986) A glutathione transferase in human leukocytes as a marker for the susceptibility to lung cancer. Carcinogenesis 7, 751-753.
14. 14. Warholm, M., Guthenberg, C., Mannervik, B., von Bahr, C., and Glaumann, H. (1980) Identification of a new glutathione S-transferase in human liver. Acta Chem. Scand. B34, 607-610.
15. 15. Seidegård, J., Vorachek, W. R., Pero, R. W., and Pearson, W. R. (1988) Hereditary differences in the expression of the human glutathione transferase active on trans-stilbene oxide are due to a gene deletion. Proc. Natl. Acad. Sci. USA 85, 7293-7297.
16. 16. Widersten, M., and Mannervik, B. (1995) Glutathione transferases with novel active sites isolated by phage display from a library of active site mutants. J. Mol. Biol. 250, 115-122.
17. 17. Hao, X.-Y., Castro, V. M., Bergh, J., Sundström, B., and Mannervik, B. (1994) Isoenzyme-specific quantitative immunoassays for cytosolic glutathione transferases and measurements of the enzymes in blood plasma from cancer patients and in tumor cell lines. Biochim. Biophys. Acta 1225, 223-230.
18. 18. Stanley, K. K. (1983) Solubilization and immune-detection of β-galactosidase hybrid proteins carrying foreign antigenic determinants. Nucleic Acids Res. 11, 4077-4092.
19. 19. Sanger, F., Nicklen, S., and Coulson, A. R. (1977) DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 74, 5463-5467.
20. 20. Bradford, M. M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248-254.
21. 21. Habig, W. H., Pabst, M. J., and Jakoby, W. B. (1974) Glutathione S-transferases: The first enzymatic step in mercapturic acid formation. J. Biol. Chem. 249, 7130-7139.
22. 22. Laemmli, U. K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (London) 227, 680-685.
23. 23. Blum, H., Beier, H., and Gross, H. J. (1987) Improved silver staining of plant proteins, RNA and DNA in polyacrylamide gels. Electrophoresis 8, 93-99.
24. 24. Mannervik, B., and Guthenberg, C. (1981) Glutathione transferase (human placenta). Methods Enzymol. 77, 231-235.
25. 25. Itakura, K., Hirose, T., Crea, R., Riggs, A. D., Heynecker, H. L., Bolivar, F., and Boyer, H. W. (1977) Expression in E. coli of a chemically synthesized gene for the hormone somatostatin. Science 198, 1056-1063.