Construction of a human cytochrome P450 1A1:Rat NADPH-cytochrome P450 reductase fusion protein cDNA and expression in Escherichia coli, purification, and catalytic properties of the enzyme in bacterial cells and after purification

Archives of Biochemistry and Biophysics

Volumn 330, Issue 1, 1996, Pages 48-58

  Chun, Young Jin ^a   Shimada, Tsutomu ^a,b   Guengerich, F Peter ^a  

^a VANDERBILT UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b OSAKA PREFECTURAL INSTITUTE OF PUBLIC HEALTH   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

BENZO[A]PYRENE; CYTOCHROME P450; HYBRID PROTEIN; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE FERRIHEMOPROTEIN REDUCTASE;

AMINO ACID SEQUENCE; ARTICLE; BACTERIAL MEMBRANE; CATALYSIS; DRUG METABOLISM; ELECTRON TRANSPORT; ENZYME ACTIVITY; ENZYME PURIFICATION; ESCHERICHIA COLI; HYDROXYLATION; NONHUMAN; PLASMID; PRIORITY JOURNAL;

ANIMALS; BASE SEQUENCE; CARCINOGENS; CHROMATOGRAPHY, AFFINITY; CHROMATOGRAPHY, DEAE-CELLULOSE; CLONING, MOLECULAR; CYTOCHROME P-450 ENZYME SYSTEM; DNA PRIMERS; DURAPATITE; ESCHERICHIA COLI; HUMANS; MICROBIAL SENSITIVITY TESTS; MOLECULAR SEQUENCE DATA; MOLECULAR WEIGHT; MUTAGENS; NADPH-FERRIHEMOPROTEIN REDUCTASE; PLASMIDS; POLYMERASE CHAIN REACTION; PRODRUGS; RATS; RECOMBINANT FUSION PROTEINS; RESTRICTION MAPPING; SALMONELLA TYPHIMURIUM; SUBSTRATE SPECIFICITY;

EID: 0029884802     PISSN: 00039861     EISSN: None     Source Type: Journal    
DOI: 10.1006/abbi.1996.0224     Document Type: Article

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