Highly efficient and precise base editing by engineered dCas9-guide tRNA adenosine deaminase in rats

Cell Discovery

Volumn 4, Issue 1, 2018, Pages

  Ma, Yuanwu ^a,b   Yu, Lei ^a   Zhang, Xu ^a   Xin, Changpeng ^c   Huang, Shisheng ^d   Bai, Lin ^a   Chen, Wei ^a   Gao, Ran ^a   Li, Jing ^a   Pan, Shuo ^a   Qi, Xiaolong ^a   Huang, Xingxu ^d   Zhang, Lianfeng ^a,b  

^a INSTITUTE OF LABORATORY ANIMAL SCIENCE   (China)

^b PEKING UNION MEDICAL COLLEGE   (China)

^c MAX PLANCK INSTITUTE   (Germany)

^d SHANGHAITECH UNIVERSITY   (China)

Author keywords

[No Author keywords available]

Indexed keywords

ADENOSINE DEAMINASE; TRANSFER RNA;

ANIMAL EXPERIMENT; CONTROLLED STUDY; FEMALE; GENE EDITING; GENE LOCUS; LETTER; NONHUMAN; PRIORITY JOURNAL; RAT; ZYGOTE;

EID: 85050403455     PISSN: None     EISSN: 20565968     Source Type: Journal    
DOI: 10.1038/s41421-018-0047-9     Document Type: Letter

References (13)

1. Iannaccone, P. M. et al. Rats! Dis. Model Mech. 2, 206-210 (2009).
2. Smalley, E. CRISPR mouse model boom, rat model renaissance. Nat. Biotechnol. 34, 893-894 (2016).
3. Li, D. et al. Heritable gene targeting in the mouse and rat using a CRISPR-Cas system. Nat. Biotechnol. 31, 681-683 (2013).
4. Ma, Y. et al. Generating ratswith conditional alleles using CRISPR/Cas9. Cell Res. 24, 122-125 (2014).
5. Ma, Y. et al. Generation of eGFP and Cre knockin rats by CRISPR/Cas9. Febs. J. 281, 3779-3790 (2014).
6. Yoshimi, K. et al. Allele-specific genome editing and correction of diseaseassociated phenotypes in rats using the CRISPR-Cas platform. Nat. Commun. 5, 4240 (2014).
7. Kleinstiver, B. P. et al. High-fidelity CRISPR-Cas9 nucleases with no detectable genome-wide off-target effects. Nature 529, 490-495 (2016).
8. Kleinstiver, B. P. et al. Broadening the targeting range of Staphylococcus aureus CRISPR-Cas9 by modifying PAM recognition. Nat. Biotechnol. 33, 1293-1298 (2015).
9. Fu, Y. et al. Improving CRISPR-Cas nuclease specificity using truncated guide RNAs. Nat. Biotechnol. 32, 279-284 (2014).
10. Kleinstiver, B. P. et al. Engineered CRISPR-Cas9 nucleases with altered PAM specificities. Nature 523, 481-485 (2015).
11. Komor, A. C. et al. Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage. Nature 533, 420-424 (2016).
12. Gaudelli, N. M. et al. Programmable base editing of A∗T to G∗C in genomic DNA without DNA cleavage. Nature 551, 464-471 (2017).
13. Bae, S. et al. Cas-OFFinder: a fast and versatile algorithm that searches for potential off-target sites of Cas9 RNA-guided endonucleases. Bioinformatics 30, 1473-1475 (2014).