메뉴 건너뛰기




Volumn 48, Issue , 2018, Pages 63-71

Anaerobic production of medium-chain fatty alcohols via a β-reduction pathway

Author keywords

Acyl CoA reductase; Anaerobic; Beta reduction; E. coli; Fatty alcohol; Medium chain alcohol; Oleochemical; Reverse beta oxidation

Indexed keywords

ALCOHOLS; ENZYMES; ESCHERICHIA COLI; GENE EXPRESSION; GLUCOSE; METABOLIC ENGINEERING; OXIDATION;

EID: 85047869653     PISSN: 10967176     EISSN: 10967184     Source Type: Journal    
DOI: 10.1016/j.ymben.2018.05.011     Document Type: Article
Times cited : (50)

References (48)
  • 1
    • 84871952399 scopus 로고    scopus 로고
    • Carboxylic acid reductase is a versatile enzyme for the conversion of fatty acids into fuels and chemical commodities
    • Akhtar, M.K., Turner, N.J., Jones, P.R., Carboxylic acid reductase is a versatile enzyme for the conversion of fatty acids into fuels and chemical commodities. Proc. Natl. Acad. Sci. USA 110 (2013), 87–92, 10.1073/pnas.1216516110.
    • (2013) Proc. Natl. Acad. Sci. USA , vol.110 , pp. 87-92
    • Akhtar, M.K.1    Turner, N.J.2    Jones, P.R.3
  • 2
    • 0023777735 scopus 로고
    • Tightly regulated tac promoter vectors useful for the expression of unfused and fused proteins in Escherichia coli
    • Amann, E., Ochs, B., Abel, K.J., Tightly regulated tac promoter vectors useful for the expression of unfused and fused proteins in Escherichia coli. Gene 69 (1988), 301–315, 10.1016/0378-1119(88)90440-4.
    • (1988) Gene , vol.69 , pp. 301-315
    • Amann, E.1    Ochs, B.2    Abel, K.J.3
  • 4
    • 0037240787 scopus 로고    scopus 로고
    • A new Escherichia coli metabolic competency: growth on fatty acids by a novel anaerobic b-oxidation pathway
    • Campbell, J.W., Morgan-Kiss, R.M., Cronan, J.E., A new Escherichia coli metabolic competency: growth on fatty acids by a novel anaerobic b-oxidation pathway. Mol. Microbiol. 47 (2003), 793–805, 10.1046/j.1365-2958.2003.03341.x.
    • (2003) Mol. Microbiol. , vol.47 , pp. 793-805
    • Campbell, J.W.1    Morgan-Kiss, R.M.2    Cronan, J.E.3
  • 6
    • 0015612808 scopus 로고
    • Genetic regulation of octane dissimilation plasmid in Pseudomonas
    • Chakrabarty, A.M., Chou, G., Gunsalus, I.C., Genetic regulation of octane dissimilation plasmid in Pseudomonas. Proc. Natl. Acad. Sci. USA 70 (1973), 1137–1140, 10.1073/pnas.70.4.1137.
    • (1973) Proc. Natl. Acad. Sci. USA , vol.70 , pp. 1137-1140
    • Chakrabarty, A.M.1    Chou, G.2    Gunsalus, I.C.3
  • 7
    • 84905001680 scopus 로고    scopus 로고
    • Codon optimization onLine (COOL): a web-based multi-objective optimization platform for synthetic gene design
    • Chin, J.X., Chung, B.K.S., Lee, D.Y., Codon optimization onLine (COOL): a web-based multi-objective optimization platform for synthetic gene design. Bioinformatics 30 (2014), 2210–2212, 10.1093/bioinformatics/btu192.
    • (2014) Bioinformatics , vol.30 , pp. 2210-2212
    • Chin, J.X.1    Chung, B.K.S.2    Lee, D.Y.3
  • 8
    • 84896979155 scopus 로고    scopus 로고
    • In silico assessment of the metabolic capabilities of an engineered functional reversal of the β-oxidation cycle for the synthesis of longer-chain (C≥ 4) products
    • Cintolesi, A., Clomburg, J.M., Gonzalez, R., In silico assessment of the metabolic capabilities of an engineered functional reversal of the β-oxidation cycle for the synthesis of longer-chain (C≥ 4) products. Metab. Eng. 23 (2014), 100–115, 10.1016/j.ymben.2014.02.011.
    • (2014) Metab. Eng. , vol.23 , pp. 100-115
    • Cintolesi, A.1    Clomburg, J.M.2    Gonzalez, R.3
  • 9
    • 84869472029 scopus 로고    scopus 로고
    • A synthetic biology approach to engineer a functional reversal of the β-oxidation cycle
    • Clomburg, J.M., Vick, J.E., Blankschien, M.D., Rodríguez-Moyá M., Gonzalez, R., A synthetic biology approach to engineer a functional reversal of the β-oxidation cycle. ACS Synth. Biol. 1 (2012), 541–554, 10.1021/sb3000782.
    • (2012) ACS Synth. Biol. , vol.1 , pp. 541-554
    • Clomburg, J.M.1    Vick, J.E.2    Blankschien, M.D.3    Rodríguez-Moyá, M.4    Gonzalez, R.5
  • 10
    • 84957678716 scopus 로고    scopus 로고
    • A transcription activator–like effector (TALE) induction system mediated by proteolysis
    • Copeland, M.F., Politz, M.C., Johnson, C.B., Markley, A.L., Pfleger, B.F., A transcription activator–like effector (TALE) induction system mediated by proteolysis. Nat. Chem. Biol. 12 (2016), 254–260, 10.1038/nchembio.2021.
    • (2016) Nat. Chem. Biol. , vol.12 , pp. 254-260
    • Copeland, M.F.1    Politz, M.C.2    Johnson, C.B.3    Markley, A.L.4    Pfleger, B.F.5
  • 11
    • 0034612342 scopus 로고    scopus 로고
    • One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products
    • Datsenko, K.A., Wanner, B.L., One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. Proc. Natl. Acad. Sci. USA 97 (2000), 6640–6645, 10.1073/pnas.120163297.
    • (2000) Proc. Natl. Acad. Sci. USA , vol.97 , pp. 6640-6645
    • Datsenko, K.A.1    Wanner, B.L.2
  • 12
    • 79960859539 scopus 로고    scopus 로고
    • Extending carbon chain length of 1-butanol pathway for 1-hexanol synthesis from glucose by engineered Escherichia coli
    • Dekishima, Y., Lan, E.I., Shen, C.R., Cho, K.M., Liao, J.C., Extending carbon chain length of 1-butanol pathway for 1-hexanol synthesis from glucose by engineered Escherichia coli. J. Am. Chem. Soc. 133 (2011), 11399–11401, 10.1021/ja203814d.
    • (2011) J. Am. Chem. Soc. , vol.133 , pp. 11399-11401
    • Dekishima, Y.1    Lan, E.I.2    Shen, C.R.3    Cho, K.M.4    Liao, J.C.5
  • 13
    • 80051941601 scopus 로고    scopus 로고
    • Engineered reversal of the β-oxidation cycle for the synthesis of fuels and chemicals
    • Dellomonaco, C., Clomburg, J.M., Miller, E.N., Gonzalez, R., Engineered reversal of the β-oxidation cycle for the synthesis of fuels and chemicals. Nature 476 (2011), 355–359, 10.1038/nature10333.
    • (2011) Nature , vol.476 , pp. 355-359
    • Dellomonaco, C.1    Clomburg, J.M.2    Miller, E.N.3    Gonzalez, R.4
  • 14
    • 0035810938 scopus 로고    scopus 로고
    • High efficiency mutagenesis, repair, and engineering of chromosomal DNA using single-stranded oligonucleotides
    • Ellis, H.M., Yu, D., DiTizio, T., Court, D.L., High efficiency mutagenesis, repair, and engineering of chromosomal DNA using single-stranded oligonucleotides. Proc. Natl. Acad. Sci. USA 98 (2001), 6742–6746, 10.1073/pnas.121164898.
    • (2001) Proc. Natl. Acad. Sci. USA , vol.98 , pp. 6742-6746
    • Ellis, H.M.1    Yu, D.2    DiTizio, T.3    Court, D.L.4
  • 15
    • 84895827954 scopus 로고    scopus 로고
    • Translation rate is controlled by coupled trade-offs between site accessibility, selective RNA unfolding and sliding at upstream standby sites
    • Espah Borujeni, A., Channarasappa, A.S., Salis, H.M., Translation rate is controlled by coupled trade-offs between site accessibility, selective RNA unfolding and sliding at upstream standby sites. Nucleic Acids Res. 42 (2014), 2646–2659, 10.1093/nar/gkt1139.
    • (2014) Nucleic Acids Res. , vol.42 , pp. 2646-2659
    • Espah Borujeni, A.1    Channarasappa, A.S.2    Salis, H.M.3
  • 16
    • 84894166328 scopus 로고    scopus 로고
    • In: Ullmann's Encyclopedia of Industrial Chemistry. doi
    • Falbe, J., Bahrmann, H., Lipps, W., Mayer, D., Frey, G.D., 2013. Alcohols, Aliphatic, In: Ullmann's Encyclopedia of Industrial Chemistry. doi: 10.1002/14356007.a01_279.pub2.
    • (2013)
    • Falbe, J.1    Bahrmann, H.2    Lipps, W.3    Mayer, D.4    Frey, G.D.5    Aliphatic, A.6
  • 17
    • 84929376894 scopus 로고    scopus 로고
    • Enzyme function initiative-enzyme similarity tool (EFI-EST): a web tool for generating protein sequence similarity networks
    • Gerlt, J.A., Bouvier, J.T., Davidson, D.B., Imker, H.J., Sadkhin, B., Slater, D.R., Whalen, K.L., Enzyme function initiative-enzyme similarity tool (EFI-EST): a web tool for generating protein sequence similarity networks. Biochim. Biophys. Acta Proteins Proteom. 1854 (2015), 1019–1037, 10.1016/j.bbapap.2015.04.015.
    • (2015) Biochim. Biophys. Acta Proteins Proteom. , vol.1854 , pp. 1019-1037
    • Gerlt, J.A.1    Bouvier, J.T.2    Davidson, D.B.3    Imker, H.J.4    Sadkhin, B.5    Slater, D.R.6    Whalen, K.L.7
  • 19
    • 33751385366 scopus 로고
    • Chemists seek greater recognition for catalysis
    • Haggin, J., Chemists seek greater recognition for catalysis. Chem. Eng. News 71 (1993), 23–27, 10.1021/cen-v071n022.p023.
    • (1993) Chem. Eng. News , vol.71 , pp. 23-27
    • Haggin, J.1
  • 20
    • 30744471966 scopus 로고    scopus 로고
    • The β-oxidation systems of Escherichia coli and Salmonella enterica are not functionally equivalent
    • Iram, S.H., Cronan, J.E., The β-oxidation systems of Escherichia coli and Salmonella enterica are not functionally equivalent. J. Bacteriol. 188 (2006), 599–608, 10.1128/JB.188.2.599-608.2006.
    • (2006) J. Bacteriol. , vol.188 , pp. 599-608
    • Iram, S.H.1    Cronan, J.E.2
  • 21
    • 58149479236 scopus 로고    scopus 로고
    • Comparative genomics of regulation of fatty acid and branched-chain amino acid utilization in proteobacteria
    • Kazakov, A.E., Rodionov, D.A., Alm, E., Arkin, A.P., Dubchak, I., Gelfand, M.S., Comparative genomics of regulation of fatty acid and branched-chain amino acid utilization in proteobacteria. J. Bacteriol. 91 (2009), 52–64, 10.1128/JB.01175-08.
    • (2009) J. Bacteriol. , vol.91 , pp. 52-64
    • Kazakov, A.E.1    Rodionov, D.A.2    Alm, E.3    Arkin, A.P.4    Dubchak, I.5    Gelfand, M.S.6
  • 22
    • 0024428050 scopus 로고
    • Characterization of the oxygen-dependent promoter of the Vitreoscilla hemoglobin gene in Escherichia coli
    • Khosla, C., Bailey, J.E., Characterization of the oxygen-dependent promoter of the Vitreoscilla hemoglobin gene in Escherichia coli. J. Bacteriol. 171 (1989), 5995–6004, 10.1128/jb.171.11.5995-6004.1989.
    • (1989) J. Bacteriol. , vol.171 , pp. 5995-6004
    • Khosla, C.1    Bailey, J.E.2
  • 23
    • 0025116097 scopus 로고
    • Expression of recombinant proteins in Escherichia coli using an oxygen-responsive promoter
    • Khosla, C., Curtis, J.E., Bydalek, P., Swartz, J.R., Bailey, J.E., Expression of recombinant proteins in Escherichia coli using an oxygen-responsive promoter. Nat. Biotechnol., 1990.
    • (1990) Nat. Biotechnol.
    • Khosla, C.1    Curtis, J.E.2    Bydalek, P.3    Swartz, J.R.4    Bailey, J.E.5
  • 24
    • 84922429494 scopus 로고    scopus 로고
    • Synthesis of medium-chain length (C6–C10) fuels and chemicals via β-oxidation reversal in Escherichia coli
    • Kim, S., Clomburg, J.M., Gonzalez, R., Synthesis of medium-chain length (C6–C10) fuels and chemicals via β-oxidation reversal in Escherichia coli. J. Ind. Microbiol. Biotechnol. 42 (2015), 465–475, 10.1007/s10295-015-1589-6.
    • (2015) J. Ind. Microbiol. Biotechnol. , vol.42 , pp. 465-475
    • Kim, S.1    Clomburg, J.M.2    Gonzalez, R.3
  • 25
    • 85041559305 scopus 로고    scopus 로고
    • Selective production of decanoic acid from iterative reversal of β-oxidation pathway
    • Kim, S., Gonzalez, R., Selective production of decanoic acid from iterative reversal of β-oxidation pathway. Biotechnol. Bioeng. 115 (2018), 1311–1320, 10.1002/bit.26540.
    • (2018) Biotechnol. Bioeng. , vol.115 , pp. 1311-1320
    • Kim, S.1    Gonzalez, R.2
  • 26
    • 0029239706 scopus 로고
    • Simple fed-batch technique for high cell density cultivation of Escherichia coli
    • Korz, D.J., Rinas, U., Hellmuth, K., Sanders, E.A., Deckwer, W.D., Simple fed-batch technique for high cell density cultivation of Escherichia coli. J. Biotechnol. 39 (1995), 59–65.
    • (1995) J. Biotechnol. , vol.39 , pp. 59-65
    • Korz, D.J.1    Rinas, U.2    Hellmuth, K.3    Sanders, E.A.4    Deckwer, W.D.5
  • 27
    • 85013168087 scopus 로고    scopus 로고
    • Characterization of endogenous and reduced promoters for oxygen-limited processes using Escherichia coli
    • Lara, A.R., Jaén, K.E., Sigala, J.-C., Mühlmann, M., Regestein, L., Büchs, J., Characterization of endogenous and reduced promoters for oxygen-limited processes using Escherichia coli. ACS Synth. Biol. 6 (2017), 344–356, 10.1021/acssynbio.6b00233.
    • (2017) ACS Synth. Biol. , vol.6 , pp. 344-356
    • Lara, A.R.1    Jaén, K.E.2    Sigala, J.-C.3    Mühlmann, M.4    Regestein, L.5    Büchs, J.6
  • 28
    • 84869122829 scopus 로고    scopus 로고
    • Engineering Escherichia coli to synthesize free fatty acids
    • Lennen, R.M., Pfleger, B.F., Engineering Escherichia coli to synthesize free fatty acids. Trends Biotechnol. 30 (2012), 659–667, 10.1016/j.tibtech.2012.09.006.
    • (2012) Trends Biotechnol. , vol.30 , pp. 659-667
    • Lennen, R.M.1    Pfleger, B.F.2
  • 29
    • 84937538704 scopus 로고    scopus 로고
    • Metabolic engineering of Escherichia coli using CRISPR-Cas9 meditated genome editing
    • Li, Y., Lin, Z., Huang, C., Zhang, Y., Wang, Z., Tang, Y., Chen, T., Zhao, X., Metabolic engineering of Escherichia coli using CRISPR-Cas9 meditated genome editing. Metab. Eng. 31 (2015), 13–21, 10.1016/j.ymben.2015.06.006.
    • (2015) Metab. Eng. , vol.31 , pp. 13-21
    • Li, Y.1    Lin, Z.2    Huang, C.3    Zhang, Y.4    Wang, Z.5    Tang, Y.6    Chen, T.7    Zhao, X.8
  • 30
    • 84865592819 scopus 로고    scopus 로고
    • A selection platform for carbon chain elongation using the CoA-dependent pathway to produce linear higher alcohols
    • Machado, H.B., Dekishima, Y., Luo, H., Lan, E.I., Liao, J.C., A selection platform for carbon chain elongation using the CoA-dependent pathway to produce linear higher alcohols. Metab. Eng. 14 (2012), 504–511, 10.1016/j.ymben.2012.07.002.
    • (2012) Metab. Eng. , vol.14 , pp. 504-511
    • Machado, H.B.1    Dekishima, Y.2    Luo, H.3    Lan, E.I.4    Liao, J.C.5
  • 31
    • 78951480559 scopus 로고    scopus 로고
    • Lambda red recombineering in Escherichia coli occurs through a fully single-stranded intermediate
    • Mosberg, J.A., Lajoie, M.J., Church, G.M., Lambda red recombineering in Escherichia coli occurs through a fully single-stranded intermediate. Genetics 186 (2010), 791–799, 10.1534/genetics.110.120782.
    • (2010) Genetics , vol.186 , pp. 791-799
    • Mosberg, J.A.1    Lajoie, M.J.2    Church, G.M.3
  • 33
  • 34
    • 84923921660 scopus 로고    scopus 로고
    • Metabolic engineering strategies for microbial synthesis of oleochemicals
    • Pfleger, B.F., Gossing, M., Nielsen, J., Metabolic engineering strategies for microbial synthesis of oleochemicals. Metab. Eng. 29 (2015), 1–11, 10.1016/j.ymben.2015.01.009.
    • (2015) Metab. Eng. , vol.29 , pp. 1-11
    • Pfleger, B.F.1    Gossing, M.2    Nielsen, J.3
  • 35
    • 84874687019 scopus 로고    scopus 로고
    • Repurposing CRISPR as an RNA-guided platform for sequence-specific control of gene expression
    • Qi, L.S., Larson, M.H., Gilbert, L.A., Doudna, J.A., Weissman, J.S., Arkin, A.P., Lim, W.A., Repurposing CRISPR as an RNA-guided platform for sequence-specific control of gene expression. Cell 152 (2013), 1173–1183, 10.1016/j.cell.2013.02.022.
    • (2013) Cell , vol.152 , pp. 1173-1183
    • Qi, L.S.1    Larson, M.H.2    Gilbert, L.A.3    Doudna, J.A.4    Weissman, J.S.5    Arkin, A.P.6    Lim, W.A.7
  • 36
    • 85032928036 scopus 로고    scopus 로고
    • Genome sequence and analysis of Escherichia coli production strain LS5218
    • Rand, J.M., Gordon, G.C., Mehrer, C.R., Pfleger, B.F., Genome sequence and analysis of Escherichia coli production strain LS5218. Metab. Eng. Commun. 5 (2017), 78–83, 10.1016/j.meteno.2017.10.001.
    • (2017) Metab. Eng. Commun. , vol.5 , pp. 78-83
    • Rand, J.M.1    Gordon, G.C.2    Mehrer, C.R.3    Pfleger, B.F.4
  • 37
    • 84927514444 scopus 로고    scopus 로고
    • Evolution for exogenous octanoic acid tolerance improves carboxylic acid production and membrane integrity
    • Royce, L.A., Yoon, J.M., Chen, Y., Rickenbach, E., Shanks, J.V., Jarboe, L.R., Evolution for exogenous octanoic acid tolerance improves carboxylic acid production and membrane integrity. Metab. Eng. 29 (2015), 180–188, 10.1016/j.ymben.2015.03.014.
    • (2015) Metab. Eng. , vol.29 , pp. 180-188
    • Royce, L.A.1    Yoon, J.M.2    Chen, Y.3    Rickenbach, E.4    Shanks, J.V.5    Jarboe, L.R.6
  • 38
    • 84982813844 scopus 로고    scopus 로고
    • Production of 1-decanol by metabolically engineered Yarrowia lipolytica
    • Rutter, C.D., Rao, C.V., Production of 1-decanol by metabolically engineered Yarrowia lipolytica. Metab. Eng. 38 (2016), 139–147, 10.1016/j.ymben.2016.07.011.
    • (2016) Metab. Eng. , vol.38 , pp. 139-147
    • Rutter, C.D.1    Rao, C.V.2
  • 39
    • 0004136246 scopus 로고    scopus 로고
    • Molecular Cloning: A Laboratory Manual
    • Cold Spring Harbor Laboratory Press New York
    • Sambrook, J., Russell, D.W., David, W., Molecular Cloning: A Laboratory Manual. 2001, Cold Spring Harbor Laboratory Press, New York.
    • (2001)
    • Sambrook, J.1    Russell, D.W.2    David, W.3
  • 40
    • 0020371936 scopus 로고
    • Translational coupling at an intercistronic boundary of the Escherichia coli galactose operon
    • Schümperli, D., McKenney, K., Sobieski, D.A., Rosenberg, M., Translational coupling at an intercistronic boundary of the Escherichia coli galactose operon. Cell 30 (1982), 865–871, 10.1016/0092-8674(82)90291-4.
    • (1982) Cell , vol.30 , pp. 865-871
    • Schümperli, D.1    McKenney, K.2    Sobieski, D.A.3    Rosenberg, M.4
  • 42
    • 79955611425 scopus 로고    scopus 로고
    • Driving forces enable high-titer anaerobic 1-butanol synthesis in Escherichia coli
    • Shen, C.R., Lan, E.I., Dekishima, Y., Baez, A., Cho, K.M., Liao, J.C., Driving forces enable high-titer anaerobic 1-butanol synthesis in Escherichia coli. Appl. Environ. Microbiol. 77 (2011), 2905–2915, 10.1128/AEM.03034-10.
    • (2011) Appl. Environ. Microbiol. , vol.77 , pp. 2905-2915
    • Shen, C.R.1    Lan, E.I.2    Dekishima, Y.3    Baez, A.4    Cho, K.M.5    Liao, J.C.6
  • 44
    • 84964489688 scopus 로고    scopus 로고
    • Room temperature electrocompetent bacterial cells improve DNA transformation and recombineering efficiency
    • Tu, Q., Yin, J., Fu, J., Herrmann, J., Li, Y., Yin, Y., Stewart, A.F., Müller, R., Zhang, Y., Room temperature electrocompetent bacterial cells improve DNA transformation and recombineering efficiency. Sci. Rep. 6 (2016), 1–8, 10.1038/srep24648.
    • (2016) Sci. Rep. , vol.6 , pp. 1-8
    • Tu, Q.1    Yin, J.2    Fu, J.3    Herrmann, J.4    Li, Y.5    Yin, Y.6    Stewart, A.F.7    Müller, R.8    Zhang, Y.9
  • 45
    • 65549089961 scopus 로고    scopus 로고
    • Purification, characterization, and potential bacterial wax production role of an nadph-dependent fatty aldehyde reductase from Marinobacter aquaeolei VT8
    • Wahlen, B.D., Oswald, W.S., Seefeldt, L.C., Barney, B.M., Purification, characterization, and potential bacterial wax production role of an nadph-dependent fatty aldehyde reductase from Marinobacter aquaeolei VT8. Appl. Environ. Microbiol. 75 (2009), 2758–2764, 10.1128/AEM.02578-08.
    • (2009) Appl. Environ. Microbiol. , vol.75 , pp. 2758-2764
    • Wahlen, B.D.1    Oswald, W.S.2    Seefeldt, L.C.3    Barney, B.M.4
  • 46
    • 82455219013 scopus 로고    scopus 로고
    • Characterization of a fatty acyl-CoA reductase from Marinobacter aquaeolei VT8: a bacterial enzyme catalyzing the reduction of fatty acyl-CoA to fatty alcohol
    • Willis, R.M., Wahlen, B.D., Seefeldt, L.C., Barney, B.M., Characterization of a fatty acyl-CoA reductase from Marinobacter aquaeolei VT8: a bacterial enzyme catalyzing the reduction of fatty acyl-CoA to fatty alcohol. Biochemistry 50 (2011), 10550–10558, 10.1021/bi2008646.
    • (2011) Biochemistry , vol.50 , pp. 10550-10558
    • Willis, R.M.1    Wahlen, B.D.2    Seefeldt, L.C.3    Barney, B.M.4
  • 47
    • 84859904363 scopus 로고    scopus 로고
    • Kinetic modeling of free fatty acid production in Escherichia coli based on continuous cultivation of a plasmid free strain
    • Youngquist, J.T., Lennen, R.M., Ranatunga, D.R., Bothfeld, W.H., Ii, W.D.M., Pfleger, B.F., Kinetic modeling of free fatty acid production in Escherichia coli based on continuous cultivation of a plasmid free strain. Biotechnol. Bioeng. 109 (2012), 1518–1527, 10.1002/bit.24420.
    • (2012) Biotechnol. Bioeng. , vol.109 , pp. 1518-1527
    • Youngquist, J.T.1    Lennen, R.M.2    Ranatunga, D.R.3    Bothfeld, W.H.4    Ii, W.D.M.5    Pfleger, B.F.6


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.