Spontaneous apoptosis in primary cultures of human and rat hepatocytes: Molecular mechanisms and regulation by dexamethasone

Cell Death and Differentiation

Volumn 9, Issue 9, 2002, Pages 945-955

  Bailly Maitre, B ^a,d   de Sousa, G ^a   Zucchini, N ^a   Gugenheim, J ^b   Boulukos, K E ^c   Rahmani, R ^a  

^a CEMAGREF   (France)

^b Laboratoire de Chirurgie Experimentale Facult de Medecine   (France)

^c UNIVERSITÉ CÔTE D'AZUR   (France)

^d BURNHAM INSTITUTE   (United States)

Author keywords

Apoptosis; Bcl 2 family; Caspase 8; Caspase 9; Cytochrome c; Primary human and rat hepatocytes

Indexed keywords

CASPASE; CASPASE 3; CASPASE 9; CYTOCHROME C; DEXAMETHASONE; HEAT SHOCK PROTEIN; HEAT SHOCK PROTEIN 27; HEAT SHOCK PROTEIN 70; MYC PROTEIN; PROTEIN BAD; PROTEIN BAX; PROTEIN BCL 2; PROTEIN BCL XL;

ANIMAL CELL; APOPTOSIS; ARTICLE; CELL SURVIVAL; CONTROLLED STUDY; DOSE RESPONSE; DRUG SCREENING; ENZYME ACTIVATION; GENE TRANSLOCATION; HUMAN; HUMAN CELL; LIVER CELL; LIVER CELL CULTURE; MITOCHONDRIAL RESPIRATION; MOLECULAR MECHANICS; NONHUMAN; ORGAN PRESERVATION; ORGAN TRANSPLANTATION; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN SECRETION; RAT; REGULATORY MECHANISM;

ANIMALS; APOPTOSIS; BCL-2-ASSOCIATED X PROTEIN; BCL-ASSOCIATED DEATH PROTEIN; BCL-X PROTEIN; CARRIER PROTEINS; CASPASES; CELL SURVIVAL; CELLS, CULTURED; CELLULAR APOPTOSIS SUSCEPTIBILITY PROTEIN; CYTOCHROME C GROUP; DEXAMETHASONE; DOSE-RESPONSE RELATIONSHIP, DRUG; DOWN-REGULATION; FAS LIGAND PROTEIN; HEPATOCYTES; HUMANS; LIVER; LIVER DISEASES; MEMBRANE GLYCOPROTEINS; MITOCHONDRIA; PROTEIN TRANSPORT; PROTO-ONCOGENE PROTEINS; PROTO-ONCOGENE PROTEINS C-BCL-2; PROTO-ONCOGENE PROTEINS C-MYC; RATS; SIGNAL TRANSDUCTION;

ANIMALIA;

EID: 85047697195     PISSN: 13509047     EISSN: None     Source Type: Journal    
DOI: 10.1038/sj.cdd.4401043     Document Type: Article

References (60)

1. Dysregulation of apoptosis as a mechanism of liver disease: An overview
2. Liver regeneration versus direct hyperplasia
3. The Sirens' song
4. Liver apoptosis
5. Drug discovery opportunities from apoptosis research
6. Cultured rat hepatocytes
7. Nitric oxide inhibits apoptosis by preventing increases in caspase-3-like activity via two distinct mechanisms
8. Effects of epidermal growth factor on CYP inducibility by xenobiotics, DNA replication, and caspase activations in collagen I gel sandwich cultures of rat hepatocytes
9. Dexamethasone inhibits spontaneous apoptosis in primary cultures of human and rat hepatocytes via Bcl-2 and Bcl-xL induction
10. Apoptosis by death factor
11. A caspase-activated DNase that degrades DNA during apoptosis, and its inhibitor ICAD
12. Targeted disruption of the mouse Caspase 8 gene ablates cell death induction by the TNF receptors, Fas/Apo1, and DR3 and is lethal prenatally
13. Differential requirement for caspase 9 in apoptotic pathways in vivo
14. Cytochrome c and dATP-dependent formation of Apaf-1/caspase-9 complex initiates an apoptotic protease cascade
15. Essential role of CED-4 oligomerizationin CED-3 activation and apoptosis
16. There lease of cytochromec from mitochondria: A primary site for Bcl-2 regulation of apoptosis
17. Bcl-2 family proteins regulate the release of apoptogenic cytochrome c by the mitochondrial channel VDAC
18. A matter of life and cell death
19. High expression of the proliferation and apoptosis associated CSE1L/CAS gene in hepatitis and liver neoplasms: Correlation with tumor progression
20. Cellular apoptosis susceptibility gene expression in endometrial carcinoma: Correlation with Bcl-2, Bax and caspase-3 expression and outcome
21. TIA-1 is a translational silencer that selectively regulates the expression of TNF-alpha
22. Apoptosis in the pathogenesis and treatment of disease
23. Essential requirement for caspase-8/FLICE in the initiation of the Fas-induced apoptotic cascade
24. Reduced apoptosis and cytochrome c-mediated caspase activation in mice lacking caspase 9
25. FLICE is predominantly expressed as two functionally active isoforms, caspase-8/a and caspase-8/b
26. Human ICE/CED-3 protease nomenclature
27. Sequential activation of ICE-like and CPP32-like proteases during Fas-mediated apoptosis
28. Inhibition of ICE family proteases by baculovirus antiapoptotic protein p35
29. The RNA-binding protein TIAR is translocated from the nucleus to the cytoplasm during Fas-mediated apoptotic cell death
30. Heat shock proteins: Endogenous modulators of apoptotic cell death
31. Cytochrome c: Can't live with it-can't live without it
32. BCL-2 family regulators of cell death
33. Akt phosphorylation of BAD couples survival signals to the cell-intrinsic death machinery
34. Interleukin-3-induced phosphorylation of BAD through the protein kinase Akt
35. Serine phosphorylation of death agonist BAD in response to survival factor results in binding to 14-3-3 not BCL-X(L)
36. Mitochondria as the central control point of apoptosis
37. Transient expression of c-fos and constant expression of c-myc in freshly isolated and cultured normal adult rat hepatocytes
38. Growth factor dependence of progression through G1 and S phases of adult rat hepatocytes in vitro. Evidence of a mitogen restriction point in mid-late G1
39. Inhibition of c-Myc expression sensitizes hepatocytes to tumor necrosis factor-induced apoptosis and necrosis
40. Mitochondria: The suicide organelles
41. Mitochondrial control of cell death
42. Nitric oxide prevents tumor necrosis factor alpha-induced rat hepatocyte apoptosis by the interruption of mitochondrial apoptotic signaling through S-nitrosylation of caspase-8
43. Activation of caspase-8 intransforming growth factor-beta-induced apoptosis of human hepatoma cells
44. Differential modulation of apoptosis sensitivity in CD95 type I and type II cells
45. Serial killers: Ordering caspase activation events in apoptosis
46. Ordering the cytochrome c-initiated caspase cascade: Hierarchical activation of caspases-2, -3, -6, -7, -8, and -10 in a caspase-9-dependent manner
47. Apaf1 is required for mitochondrial pathways of apoptosis and brain development
48. The Bcl-2 protein family: Arbiters of cell survival
49. All along the watchtower: On the regulation of apoptosis regulators
50. Interference of BAD (Bcl-xL/Bcl-2-associated death promoter)-induced apoptosis in mammalian cells by 14-3-3 isoforms and P11
51. Fas-mediated apoptosis in mouse hepatocytes involves the processing and activation of caspases
52. FLICE, a novel FADD-homologous ICE/CED-3-like protease, is recruited to the CD95 (Fas/APO-1) death-inducing signaling complex
53. Regulation of CD95 (APO-1/Fas) receptor and ligand expression by lipopolysaccharide and dexamethasone in parenchymal and nonparenchymal rat liver cells
54. Lethal effect of the anti-Fas antibody in mice
55. Transcriptional regulation of the bcl-x gene encoding the anti-apoptotic Bcl-xL protein by Ets, Rel/NFkappaB, STAT and AP1 transcription factor families
56. High-yield preparation of isolated rat liver parenchymal cells: A biochemical and fine structural study
57. Hepatic biotransformation of docetaxel (Taxotere) in vitro: Involvement of the CYP3A subfamily in humans
58. Reactive oxygen species (ROS) mediates the mitochondrial-dependent apoptosis induced by transforming growth factor (beta) in fetal hepatocytes
59. Fluorometric and colorimetric detection of caspase activity associated with apoptosis
60. Measurement of protein using bicinchoninic acid