Characterization of a new GmFAD3A allele in Brazilian CS303TNKCA soybean cultivar

Theoretical and Applied Genetics

Volumn 131, Issue 5, 2018, Pages 1099-1110

  Silva, Luiz Claudio Costa ^a   Bueno, Rafael Delmond ^a   da Matta, Loreta Buuda ^b   Pereira, Pedro Henrique Scarpelli ^c   Mayrink, Danyelle Barbosa ^a   Piovesan, Newton Deniz ^a   Sediyama, Carlos Sigueyuki ^a   Fontes, Elizabeth Pacheco Batista ^a   Cardinal, Andrea J ^d,e   Dal Bianco, Maximiller ^a  

^a FEDERAL UNIVERSITY OF VIÇOSA   (Brazil)

^b Seeds Bayer Crop Science   (Brazil)

^c UNIVERSITY OF SÃO PAULO   (Brazil)

^d NORTH CAROLINA STATE UNIVERSITY   (United States)

^e SYNGENTA BIOTECHNOLOGY INC   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CULTIVATION; FORESTRY; GENES; PALMITIC ACID; POLYUNSATURATED FATTY ACIDS; SOYBEAN OIL;

BREEDING PROGRAM; ECONOMIC VALUES; ENZYMATIC ACTIVITIES; GENOTYPING ASSAYS; HETEROLOGOUS EXPRESSION; MOLECULAR MARKER; OXIDATIVE STABILITY; PHENOTYPIC VARIATIONS;

LINOLEIC ACID;

LINOLEIC ACID; SOYBEAN OIL;

ALLELE; AMINO ACID SEQUENCE; BRAZIL; CHEMISTRY; CROSS BREEDING; GENETICS; GENOTYPE; PHENOTYPE; PLANT BREEDING; PLANT GENE; POINT MUTATION; SOYBEAN; STOP CODON;

ALLELES; AMINO ACID SEQUENCE; BRAZIL; CODON, NONSENSE; CROSSES, GENETIC; GENES, PLANT; GENOTYPE; LINOLEIC ACID; PHENOTYPE; PLANT BREEDING; POINT MUTATION; SOYBEAN OIL; SOYBEANS;

EID: 85044918236     PISSN: 00405752     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00122-018-3061-2     Document Type: Article

References (70)

1. ABIOVE (2017) Estatística Mensal do Complexo Soja
2. Alt JL, Fehr WR, Welke GA, Shannon JG (2005) Transgressive segregation for oleate content in three soybean populations. Crop Sci 45:2005–2007
3. Anai T, Yamada T, Kinoshita T, Rahman SM, Takagi Y (2005) Identification of corresponding genes for three low-α-linolenic acid mutants and elucidation of their contribution to fatty acid biosynthesis in soybean seed. Plant Sci 168:1615–1623
4. Andersen CL, Jensen JL, Orntoft TF (2004) Normalization of real-time quantitative reverse transcription-PCR data: a model-based variance estimation approach to identify genes suited for normalization, applied to bladder and colon cancer data sets. Cancer Res 64:5245–5250
5. Artimo P, Jonnalagedda M, Arnold K, Baratin D, Csardi G, de Castro E, Duvaud S, Flegel V, Fortier A, Gasteiger E, Grosdidier A, Hernandez C, Ioannidis V, Kuznetsov D, Liechti R, Moretti S, Mostaguir K, Redaschi N, Rossier G, Xenarios I, Stockinger H (2012) ExPASy: SIB bioinformatics resource portal. Nucl Acids Res 40:W597–603
6. Bachleda N, Pham AT, Li Z (2016) Identifying FATB1a deletion that causes reduced palmitic acid content in soybean N87-2122-4 to develop a functional marker for marker-assisted selection. Mol Breed 36(4):45
7. Baud S, Lepiniec L (2010) Physiological and developmental regulation of seed oil production. Prog Lipid Res 49:235–249
8. Bilyeu KD, Palavalli L, Sleper DA, Beuselinck PR (2003) Three microsomal omega-3 fatty-acid dessaturase genes contribute to soybean linolenic acid levels. Crop Sci 43:1833–1838
9. Bilyeu KD, Palavalli L, Sleper DA, Beuselinck PR (2005) Mutations in soybean microsomal omega-3 fatty acid dessaturase genes reduce linolenic acid concentration in soybean seeds. Crop Sci 45(5):1830–1836
10. Bilyeu KD, Palavalli L, Sleper DA, Beuselinck PR (2006) Molecular genetic resources for development of 1% linolenic acid soybeans. Crop Sci 46:1913–1918
11. Bilyeu KD, Gillman JD, Leroy AR (2011) Novel FAD3 mutant allele combinations produce soybeans containing 1% linolenic acid in the seed oil. Crop Sci 52:259–264
12. Brazil (2017) Lei nº 13.263, de março de 2016
13. Bruner AC, Jung S, Abbott AG, Powell GL (2001) The naturally occurring high oleate oil character in some peanut varieties results from reduced oleoyl-PC desaturase activity from mutation of aspartate 150 to asparagine. This work was partially supported by grants from the South Carolina Exp. Stn., Clemson Univ., and by a grant from AgraTech Seeds Inc., Ashburn, GA. Crop Sci 41:522–526
14. Bubeck DM, Fehr WR, Hammond EG (1989) Inheritance of palmitic and stearic acid mutants of soybean. Crop Sci 29:562–656
15. Burkey KO, Booker FL, Pursley WA, Heagle AS (2007) Elevated carbon dioxide and ozone effects on peanut: II. Seed Yield and Quality. Crop Sci 47:1488–1497
16. Byrum JR, Kinney AJ, Stecca KL, Grace DJ, Diers BW (1997) Alteration of the omega-3 fatty acid desaturase gene is associated with reduced linolenic acid in the A5 soybean genotype. Theor Appl Genet 94:4
17. Cardinal A, Burton JW, Camacho-Roger AM, Whetter R, Chappell AS, Bilyeu KD, Auclair J, Dewey RE (2011) Molecular analysis of GmFAD3A in two soybean populations segregating for the fan, fap1, and fapnc loci. Crop Sci 51(5):2104–2112
18. Chapell AS, Bilyeu KD (2006) A GmFAD3A mutation in the low linolenic acid soybean line C1640. Plant Breed 125:535–536
19. CONAB (2017) Acompanhamento da Safra Brasileira. SAFRA, p 164
20. Cruz CD (2013) Genes—a software package for analysis in experimental statistics and quantitative genetics. Acta Sci Agron 35:5
21. Doyle JJ, Doyle JL (1990) Isolation of plant DNA from fresh tissue. Focus 12:13–15
22. Dunton HJ, Lancaster CR, Evans CD, Cowan JC (1951) The flavor problem of soybean oil VII. Linolenic acid. J Am Oil Chem 28:115–118
23. FDA (2015) Final determination regarding partially hydrogenated oils (removing trans fat)
24. FDA (2016) Constituent update: FDA takes step to remove artificial trans fats from processed foods
25. Fehr WR (2007) Breeding for modified fatty acid composition in soybean. Crop Sci 47:72–87
26. Fehr WR, Hammond EG (2000) Reduced linolenic acid production in soybeans. U. S. Patent 6,133,509
27. Flores T, Karpova O, Su X, Zeng P, Bilyeu K, Sleper DA, Nguyen HT, Zhang ZJ (2008) Silencing of GmFAD3 gene by siRNA leads to low alpha-linolenic acids (18:3) of fad3-mutant phenotype in soybean [Glycine max (Merr.)]. Trans Res 17(5):839–850
28. Gietz RD, Schiestl RH (2007) High-efficiency yeast transformation using the LiAc/SS carrier DNA/PEG method. Nat Protoc 2(1):31
29. Goettel W, Xia E, Upchurch R, Wang ML, Chen P, An YQ (2014) Identification and characterization of transcript polymorphisms in soybean lines varying in oil composition and content. BMC Genom 15:299
30. Goodstein DM, Shu S, Howson R, Neupane R, Hayes RD, Fazo J, Mitros T, Dirks W, Hellsten U, Putnam N, Rokhsar DS (2012) Phytozome: a comparative platform for green plant genomics. Nucl Acids Res Database Issue 40:D1178–1186
31. Hammond EG, Fehr WR (1983) Registration of A5 germplasm line of soybean (Reg. No. GP44). Crop Sci 23:192–193
32. Hellemans J, Mortier G, De Paepe A, Speleman F, Vandesompele J (2007) qBase relative quantification framework and software for management and automated analysis of real-time quantitative PCR data. Genome Biol 8:R19
33. Hu FB, Stampfer MJ, Manson JE, Rimm E, Colditz GA, Rosner BA, Hennekens CH, Willett WC (1997) Dietary fat intake and the risk of coronary heart disease in women. N Engl J Med 337:1491–1499
34. Hu R, Fan C, Li H, Zhang Q, Fu YF (2009) Evaluation of putative reference genes for gene expression normalization in soybean by quantitative real-time RT-PCR. BMC Mol Biol 10:93
35. Le DT, Aldrich DL, Valliyodan B, Watanabe Y, Ha CV, Nishiyama R, Guttikonda SK, Quach TN, Gutierrez-Gonzalez JJ, Tran LS, Nguyen HT (2012) Evaluation of candidate reference genes for normalization of quantitative RT-PCR in soybean tissues under various abiotic stress conditions. PLoS ONE 7:e46487
36. Leffel RC (1994a) Registration of BARC-12 a low linolenic acid soybean germplasm line. Crop Sci 34:1426–1427
37. Leffel RC (1994b) Registration of BARC-12 a low linolenic acid soybean germplasm line. Crop Sci 34:1426–1427
38. Li L, Wang X, Gai J, Yu D (2007) Molecular cloning and characterization of a novel microsomal oleate desaturase gene from soybean. J Plant Physiol 164:1516–1526
39. Li Q, Fan CM, Zhang XM, Fu YF (2012) Validation of reference genes for real-time quantitative PCR normalization in soybean developmental and germinating seeds. Plant Cell Rep 31:1789–1798
40. Libault M, Thibivilliers S, Bilgin DD, Radwan O, Benitez M, Clough SJ, Stacey G (2008) Identification of four soybean reference genes for gene expression normalization. Plant Genome 1:44–54
41. Lozinsky S, Yang H, Forseille L, Cook GR, Ramirez-Erosa I, Smith MA (2014) Characterization of an oleate 12-desaturase from Physaria fendleri and identification of 5′UTR introns in divergent FAD2 family genes. Plant Physiol Biochem 75:114–122
42. Miranda Vde J, Coelho RR, Viana AA, de Oliveira Neto OB, Carneiro RM, Rocha TL, de Sa MF, Fragoso RR (2013) Validation of reference genes aiming accurate normalization of qPCR data in soybean upon nematode parasitism and insect attack. BMC Res Notes 6:196
43. Mozaffarian D, Katan MB, Ascherio A, Stampfer MJ, Willett WC (2006) Trans fatty acids and cardiovascular disease. N Engl J Med 354:1601–1613
44. Pfaffl MW, Tichopad A, Prgomet C, Neuvians TP (2004) Determination of stable housekeeping genes, differentially regulated target genes and sample integrity: BestKeeper–Excel-based tool using pair-wise correlations. Biotechnol Lett 26:509–515
45. Pham AT, Lee JD, Shannon JG, Bilyeu KD (2010) Mutant alleles of FAD2-1A and FAD2-1B combine to produce soybeans with the high oleic acid seed oil trait. BMC Plant Biol 10:195
46. Pham AT, Lee JD, Shannon JG, Bilyeu KD (2011) A novel FAD2-1 A allele in a soybean plant introduction offers an alternate means to produce soybean seed oil with 85% oleic acid content. Theor Appl Genet 123:793–802
47. Pham AT, Shannon JG, Bilyeu KD (2012) Combinations of mutant FAD2 and FAD3 genes to produce high oleic acid and low linolenic acid soybean oil. Theor Appl Genet 125:503–515
48. Pham AT, Bilyeu KD, Chen P, Boerma HR, Li Z (2014) Characterization of the fan1 locus in soybean line A5 and development of molecular assays for high-throughput genotyping of FAD3 genes. Mol Breed 33:895–907
49. Pinto MO, Good-God PIV, Moreira MA, Barros EG (2013) Association of SNP markers with the linolenic acid content in soybean seeds. Pesqui Agropecu Bras 48:263–269
50. Rahman SM, Takagi Y, Kinoshita T (1996) Genetic control of high oleic acid content in the seed oil of two soybean mutants. Crop Sci 36:1125–1128
51. Reinprecht Y, Pauls KP (2016) Microsomal omega-3 fatty acid desaturase genes in low linolenic acid soybean line RG10 and validation of major linolenic acid QTL. Front Genet 7:38
52. Reinprecht Y, Luk-Labey SY, Larsen J, Poysa VW, Yu K, Rajcan I, Ablett GR, Pauls KP (2009) Molecular basis of the low linolenic acid trait in soybean EMS mutant line RG10. Plant Breed 128:253–258
53. Ross AJ, Fehr WR, Welke GA, Hammond EG, Cianzio SR (2000) Agronomic and seed traits of 1%—linolenate soybean genotypes. Crop Sci 40:383–386
54. Rozen S, Skaletsky H (2000) Primer3 on the WWW for general users and for biologist programmers. Methods Mol Biol 132:365–386
55. Schmutz J, Cannon SB, Schlueter J, Ma J, Mitros T, Nelson W, Hyten DL, Song Q, Thelen JJ, Cheng J, Xu D, Hellsten U, May GD, Yu Y, Sakurai T, Umezawa T, Bhattacharyya MK, Sandhu D, Valliyodan B, Lindquist E, Peto M, Grant D, Shu S, Goodstein D, Barry K, Futrell-Griggs M, Abernathy B, Du J, Tian Z, Zhu L, Gill N, Joshi T, Libault M, Sethuraman A, Zhang XC, Shinozaki K, Nguyen HT, Wing RA, Cregan P, Specht J, Grimwood J, Rokhsar D, Stacey G, Shoemaker RC, Jackson SA (2010) Genome sequence of the palaeopolyploid soybean. Nature 463:178–183
56. Shanklin J, Whittle E, Fox BG (1994) Eight histidine residues are catalytically essential in a membrane-associated iron enzyme, stearoyl-CoA desaturase, and are conserved in alkane hydroxylase and xylene monooxygenase. Biochemistry 33:12787–12794
57. Shi Z, Bachleda N, Pham AT, Bilyeu KD, Shannon G, Nguyen H, Li Z (2015) High-throughput and functional SNP detection assays for oleic and linolenic acids in soybean. Mol Breed 35:176
58. Stojsin D, LuzziI BM, Ablett GR, Tanner JW (1998) Inheritance of low linolenic acid level in the soybean line RG10. Crop Sci 38:1441–1444
59. Sun R, Gao L, Yu X, Zheng Y, Li D, Wang X (2016) Identification of a Delta12 fatty acid desaturase from oil palm (Elaeis guineensis Jacq.) involved in the biosynthesis of linoleic acid by heterologous expression in Saccharomyces cerevisiae. Gene 591:21–26
60. Thompson JD, Gibson TJ, Higgins DG (2002) Multiple sequence alignment using ClustalW and ClustalX. Curr Protoc Bioinform. Chapter 2: Unit 2–3
61. USDA (2017a) Foreign agricultural service: table 03: major vegetable oils: world supply and distribution (commodity view)
62. USDA (2017b) Foreign agricultural service: world agricultural production
63. van de Mortel M, Recknor JC, Graham MA, Nettleton D, Dittman JD, Nelson RT, Godoy CV, Abdelnoor RV, Almeida AM, Baum TJ, Whitham SA (2007) Distinct biphasic mRNA changes in response to Asian soybean rust infection. Mol Plant Microbe Interact 20:887–899
64. Vandesompele J, De Preter K, Pattyn F, Poppe B, Van Roy N, De Paepe A, Speleman F (2002) Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes. Genome Biol 3:RESEARCH0034
65. Waltz E (2010) Food firms test fry Pioneer’s trans fat-free soybean oil. Nat Biotechnol 28:769–770
66. Warner K, Fehr W (2008) Mid-oleic/ultra low linolenic acid soybean oil: a healthful new alternative to hydrogenated oil for frying. J Am Oil Chem Soc 95:945–951
67. White HB, Quakenbush FW, Probst AH (1961) Occurrence and inheritance of linolenic and linoleic acids in soybean seeds. J Am Oil Chem 38:113–117
68. Wilcox JR, Cavins JF, Nielsen NC (1984) Genetic alteration of soybean oil compositon by a chemical mutagen. J Am Oil Chem 61:97–100
69. Wilson RF, Burton JW, Brim CA (1981) Progress in the selection for altered fatty acid composition in soybeans. Crop Sci 21:788–791
70. Yadav NS (1996) Soybean genetics, molecular biology and biotechnology. Biotechnology in Agriculture Series. CAB International