Characterization of an oleate 12-desaturase from Physaria fendleri and identification of 5'UTR introns in divergent FAD2 family genes

Plant Physiology and Biochemistry

Volumn 75, Issue , 2014, Pages 114-122

  Lozinsky, Sharla ^a   Yang, Hui ^a   Forseille, Li ^a   Cook, Gillian R ^a   Ramirez Erosa, Irving ^a   Smith, Mark A ^a  

^a NATIONAL RESEARCH COUNCIL CANADA   (Canada)

Author keywords

FAD2; Fatty acid desaturase; Fatty acid hydroxylase; Lesquerella5'UTR intron; Physaria fendleri

Indexed keywords

ACYL COENZYME A DESATURASE; FATTY ACID DESATURASE 2; FATTY ACID HYDROXYLASE; HYDROXY FATTY ACID; MIXED FUNCTION OXIDASE; OLEIC ACID; ORF; OXIDOREDUCTASE; PHOSPHATIDYLCHOLINE 12 MONOOXYGENASE; PHOSPHATIDYLCHOLINE 12-MONOOXYGENASE; PHOSPHATIDYLCHOLINE DESATURASE; VEGETABLE PROTEIN;

5' UNTRANSLATED REGION; 5′UTR INTRON; AMINO ACID SEQUENCE; ARABIDOPSIS; ARTICLE; BRASSICACEAE; DNA SEQUENCE; EST; EXPRESSED SEQUENCE TAG; FAD2; FAMES; FATTY ACID METHYL ESTERS; GENETICS; HFA; INTRON; LESQUERELLA; METABOLISM; MOLECULAR GENETICS; OPEN READING FRAME; PHYSARIA FENDLERI; PLANT GENE; PLANT LEAF; PLANT SEED; RICINUS COMMUNIS; SEQUENCE HOMOLOGY; START CODON;

5′UTR INTRON; EST; EXPRESSED SEQUENCE TAG; FAD2; FAMES; FATTY ACID DESATURASE; FATTY ACID DESATURASE 2; FATTY ACID HYDROXYLASE; FATTY ACID METHYL ESTERS; HFA; HYDROXY FATTY ACID; LESQUERELLA; OPEN READING FRAME; ORF; PHYSARIA FENDLERI;

5' UNTRANSLATED REGIONS; AMINO ACID SEQUENCE; ARABIDOPSIS; BRASSICACEAE; CODON, INITIATOR; EXPRESSED SEQUENCE TAGS; FATTY ACID DESATURASES; GENES, PLANT; INTRONS; MIXED FUNCTION OXYGENASES; MOLECULAR SEQUENCE DATA; OLEIC ACID; OXIDOREDUCTASES ACTING ON CH-CH GROUP DONORS; PLANT LEAVES; PLANT PROTEINS; RICINUS; SEEDS; SEQUENCE ANALYSIS, DNA; SEQUENCE HOMOLOGY;

EID: 84892399180     PISSN: 09819428     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.plaphy.2013.12.016     Document Type: Article

References (35)

1. Al-Shehbaz I.A., O'Kane S.L. Lesquerella is United with Physaria (Brassicaceae). Novon 2002, 12:319-329.
2. Al-Shehbaz I.A., Beilstein M.A., Kellogg E.A. Systematics and phylogeny of the Brassicaceae (Cruciferae): an overview. Pl. Syst. Evol. 2006, 259:89-120.
3. Bailey C.D., Koch M.A., Mayer M., Mummenhoff K., O'Kane S.L., Warwick S.I., Windham M.D., Al-Shehbaz I.A. Towards a global phylogeny of the Brassicaceae. Mol. Biol. Evol. 2006, 23:2142-2160.
4. Broun P., Boddupalli S., Somerville C. Abifunctional oleate 12-hydroxylase: desaturase from Lesquerella fendleri. Plant J. 1998, 13:201-210.
5. Broun P., Shanklin J., Whittle E., Somerville C. Catalytic plasticity of fatty acid modification enzymes underlying chemical diversity of plant lipids. Science 1998, 282:1315-1317.
6. Brown J.W.S., Smith P., Simpson C.G. Arabidopsis consensus intron sequences. Plant Mol. Biol. 1996, 32:531-535.
7. Brown A.P., Kroon J.T.M., Swarbreck D., Febrer M., Larson T.R., Graham I.A., Caccamo M., Slabas A.R. tissue-specific whole transcriptome sequencing in castor, directed at understanding triacylglycerol lipid biosynthetic pathways. PLOS One 2012, 7:e30100.
8. Cao S., Zhou X.-J., Wood C.C., Green A.G., Singh S.P., Liu L., Liu Q. Alarge and functionally diverse family of FAD2 genes in safflower (Carthamus tinctorius L.). BMC Plant Biol. 2013, 13:5.
9. Chan A.P., Crabtree J., Zhao Q., Lorenzi H., Orvis J., Puiu D., Melake-Berhan A., Jones K.M., Redman J., Chen G., Cahoon E.B., Gedil M., Stanke M., Haas B.J., Wortman J.R., Fraser-Liggett C.M., Ravel J., Rabinowicz P.D. Draft genome sequence of the oilseed species Ricinus communis. Nat. Biotechnol. 2010, 28:951-959.
10. Chen G.Q., Lin J.-T., Lu C. Hydroxy fatty acid synthesis and lipid gene expression during seed development in Lesquerella fendleri. Ind. Crop. Prod. 2011, 34:1286-1292.
11. Christie W.W. Mass spectrometry of fatty acids with methylene-interrupted ene-yne systems. Chem. Phys. Lipids 1998, 35-41.
12. Engeseth N., Stymne S. Desaturation of oxygenated fatty acids in Lesquerella and other oil seeds. Planta 1996, 198:245-283.
13. Hayes D.G., Kleiman R., Phillips B.S. The triglyceride composition, structure, and presence of estolides in the oils of Lesquerella and related species. J.Am. Oil Chem. Soc. 1995, 5:559-569.
14. Higo K., Ugawa Y., Iwamoto M., Korenaga T. Plant cis-acting regulatory DNA elements (PLACE) dataset:1999. Nucl. Acids Res. 1999, 27:297-300.
15. Kang J., Snapp A.R., Lu C. Identification of three genes encoding microsomal oleate desaturase (FAD2) from the oilseed crop Camelina sativa. Plant Physiol. Biochem. 2011, 49:223-229.
16. Kim M.J., Kim H., Shin J.S., Chung C.-H., Ohlrogge J.B., Suh M.C. Seed-specific expression of sesame microsomal oleic acid desaturase is controlled by combinatorial properties between negative cis-regulatory elements in the SeFAD2 promoter and enhancers in the 5'-UTR intron. Mol. Gen. Genom. 2006, 276:351-368.
17. Lee K.-R., Kim S.H., Go Y.-S., Jung S.M., Roh K.H., Kim J.-B., Suh M.-C., Lee S., Kim H.U. Molecular cloning and functional analysis of two FAD2 genes from American grape (Vitis labrusca L.). Gene 2012, 509:189-194.
18. Liu Q., Brubaker C.L., Green A.G., Marshall D.R., Sharpe P.J., Singh S.P. Evolution of the FAD2-1 fatty acid desaturase 5'UTR intron and the molecular systematic of Gossypium (Malvaceae). Am. J. Bot. 2001, 88:92-102.
19. Lu C., Wallis J.G., Browse J. An analysis of expressed sequence tags of developing castor endosperm using a full-length cDNA library. BCM Plant Biol. 2007, 7:42.
20. Mayer K.M., McCorkle S.R., Shanklin J. Linking enzyme sequence to function using conserved property difference locator to identify and annotate positions likely to control specific functionality. BMC Bioinforma. 2005, 6:284.
21. McCartney A.W., Dyer J.M., Dhanoa P.K., Kim P.K., Andrews D.W., McNew J.A., Mullen R.T. Membrane-bound fatty acid desaturases are inserted co-translationally into the ER and contain different ER retrieval motifs at their carboxy termini. Plant J. 2004, 37:156-173.
22. Meisel L., Fonseca B., Gonzalez S., Baeza-Yates R., Cambiazo V., Campos R., Gonzalez M., Orellana A., Retamales J., Silva H. Arapid and efficient method for purifying high quality total RNA from peaches (Prunus persica) for functional genomic analyses. Biol. Res. 2005, 38:83-88.
23. Moon H., Smith M.A., Kunst L. Acondensing enzyme from the seeds of Lesquerella fendleri that specifically elongates hydroxy fatty acids. Plant Physiol. 2001, 127:1635-1643.
24. Mount S.M. Acatalogue of splice junction sequences. Nucl. Acids Res. 1982, 10:459-472.
25. Parra G., Bradnam K., Rose A.B., Korf I. Comparative and functional analysis of intron-mediated enhancement signals reveals conserved features among plants. Nucl. Acids Res. 2011, 39:5328-5337.
26. Prestridge D.S. SIGNAL SCAN: a computer program that scans DNA sequences for eukaryotic transcriptional elements. Comput. Appl. Biosci. 1991, 7:203-206.
27. Quinn J.M., Eriksson M., Moseley J.L., Merchant S. Oxygen deficiency responsive gene expression in Chlamydomonas reinhardtii through a copper-sensing signal transduction pathway. Plant Physiol. 2002, 128:463-471.
28. Reed D.W., Taylor D.C., Covello P.S. Metabolism of hydroxy fatty acids in developing seeds in the genera Lesquerella (Brassicaceae) and Linum (Linaceae). Plant Physiol. 1997, 114:63-68.
29. Salywon A.M., Dierig D.A., Rebman J.P., De-Rodriguez D.J. Evaluation of new Lesquerella and Physaria (Brassicaceae) oilseed germplasm. Am. J. Bot. 2005, 92:53-62.
30. Shanklin J., Cahoon E.B. Desaturation and related modification of fatty acids. Annu. Rev. Plant Physiol. Plant Mol. Biol. 1998, 49:611-641.
31. Simpson G.G., Filipowicz W. Splicing of precursors to mRNA in higher plants: mechanism, regulation and sub-nuclear organization of the spliceosomal machinery. Plant Mol. Biol. 1996, 32:1-41.
32. Sperling P., Ternes P., Zank T.K., Heinz E. The evolution of desaturases. Prostagl. Leukot. Essent. Fat. Acids 2003, 68:73-95.
33. van de Loo F.J., Broun P., Turner S., Somerville C. An oleate 12-hydroxylase from Ricinus communis L. is a fatty acyl desaturase homolog. Proc. Natl. Acad. Sci. U S A 1995, 92:6743-6747.
34. Volschenk H., Viljoen M., Grobler J., Petzold B., Bauer F., Subden R.E., Young R.A., Lonvaud A., Denayrolles M., van Vuuren H.J. Engineering pathways for malate degradation in Saccharomyces cerevisiae. Nat. Biotechnol. 1997, 15:253-257.
35. Zhang D., Pirtle I.L., Park S.J., Nampaisansuk M., Neogi P., Wanjie S.W., Pirtle R.M., Chapman K.D. Identification and expression of a new delta-12 fatty acid desaturase (FAD2-4) gene in upland cotton and its functional expression in yeast and Arabidopsis thaliana plants. Plant Physiol. Biochem. 2009, 47:462-471.