Pea protein isolates: Structure, extraction, and functionality

Food Reviews International

Volumn 34, Issue 2, 2018, Pages 126-147

  Lam, A C Y ^a   Can Karaca, A ^b   Tyler, R T ^a   Nickerson, M T ^a  

^a UNIVERSITY OF SASKATCHEWAN   (Canada)

^b Aromsa AS GOSB Ihsan Dede Cad   (Turkey)

Author keywords

Environment; functionality; genotype; isolate; legumin; pea protein; vicilin

Indexed keywords

EMULSIFICATION; EXTRACTION; GELATION; OILS AND FATS;

ENVIRONMENT; FUNCTIONALITY; GENOTYPE; ISOLATE; LEGUMIN; PEA PROTEINS; VICILIN;

PROTEINS;

EID: 85041439600     PISSN: 87559129     EISSN: 15256103     Source Type: Journal    
DOI: 10.1080/87559129.2016.1242135     Document Type: Review

References (96)

1. Can Karaca, A.,; Low, N.H.,; Nickerson, M.T., Emulsifying properties of chickpea, faba bean, lentil and pea proteins produced by isoelectric precipitation and salt extraction. Food Res. Int. 2011, 44, 2742–2750.
2. Mertens, C.,; Dehon, L.,; Bourgeois, A.,; Verhaeghe-Cartrysse, C.,; Blecker, C., Agronomical factors influencing the legumin/vicilin ratio in pea (Pisum sativum L.) seeds. J. Sci. Food Agric. 2012, 92, 1591–1596.
3. Toews, R.,; Wang, N., Physicochemical and functional properties of protein concentrates from pulses. Food Res. Int. 2013, 52, 445–451.
4. Shevkani, K.,; Singh, N.,; Kaur, A.,; Rana, J.C., Structural and functional characterization of kidney bean and field pea protein isolates: A comparative study. Food Hydrocolloids 2015, 43, 679–689.
5. Stone, A.K.,; Karalash, A.,; Tyler, R.T.,; Warkentin, T.D.,; Nickerson, M.T., Functional attributes of pea protein isolates prepared using different extraction methods and cultivars. Food Res. Int. 2015, 76, 31–38.
6. Boye, J.,; Zare, F.,; Pletch, A., Pulse proteins: Processing, characterization, functional properties and applications in food and feed. Food Res. Int. 2010, 43, 414–431.
7. Swanson, B.G., Pea and lentil protein extraction and functionality. J. Am. Oil Chem. Soc. 1990, 67(5), 276–280.
8. Adebiyi, A.P.,; Aluko, R.E., Functional properties of protein fractions obtained from Commercial yellow field pea (Pisum sativum L.) seed protein isolate. Food Chem 2011, 128, 902–908.
9. Olson, R.A.,; Frey, K.J., Nutritional quality of cereal grains: genetics and agronomic improvement; American Society of Agronomy, Inc.: Madison, WI, 1987; p 511.
10. Gueguen, J., Legume seed protein extraction, processing, and end product characteristics. Qualitas Plant. Plant Foods Hum. Nutr. 1983, 32, 267–303.
11. Tiwari, B.K.,; Gowen, A.,; McKenna, B., Processing, quality and nutraceutical applications; Elsevier Inc.: London, UK, 2011, p 483.
12. Hoover, R.,; Hughes, T.,; Chung, H.J.,; Liu, Q., Composition, molecular structure, properties, and modification of pulse starches: a review. Food Res Int 2010, 43, 399–413.
13. Duranti, M.,; Scarafoni, A., Modification of storage protein content and quality in legume seeds. J. New Seeds 1999, 1(1), 17–35.
14. Sikorski, Z.E., Chemical and functional properties of food proteins; CRC Press LLC: Boca Raton, FL, 2001, p 504.
15. Gatehouse, J.A.,; Lycett, G.W.,; Croy, R.R.D.,; Boulter, D., The post-translational proteolysis of the subunits of vicilin from pea (Pisum sativum L.). Biochem. J. 1982, 207, 629–632.
16. Tzitzikas, E.N.,; Vincken, J.P.,; de Groot, J.,; Gruppen, H.,; Visser, R.G.F., Genetic variation in pea seed globulin composition. J. Agric. Food Chem. 2006, 54, 425–433.
17. Mession, J.–L.,; Assifaoui, A.,; Cayot, P.,; Saurel, R., Effect of pea proteins extraction and vicilin/legumin, fractionation on the phase behavior in admixture with alginate. Food Hydrocolloids 2012, 29, 335–346.
18. Jackson, P.,; Boulter, D.,; Thurman, D.A., A comparison of some properties of vicilin and legumin isolated from seeds of Pisum sativum, Vicia faba and Cicer arietinum. New Phytol. 1969, 68, 25–33.
19. Boulter, D., Protein composition of grains of the leguminosae. Qualitas Plant. Plant Foods Hum. Nutri. 1983, 32, 247–52.
20. Vera, C.,; Warkentin, T.,; Vandenberg, B., 1999 final report to the Saskatchewan Agriculture Development Fund: Breeding field pea cultivars for Saskatchewan (Report No. 19940181); Agriculture and Agri-Food Canada, 2000.
21. Acikgoz, E.,; Ustun, A.,; Gul., I.,; Anlarsal, E.,; Tekeli, A.S.,; Nizam, I.,; Avcioglu, R.,; Geren, H.,; Cakmakci, S.,; Aydinoglu, B.,; Yucel, C.,; Avci, M.,; Acar, Z.,; Ayan, I.,; Uzun, A.,; Bilgili, U.,; Sincik, M.,; Yavuz, M., Genotype x environment interaction and stability analysis for dry matter and seed yield in field pea (Pisum sativum L.). Spanish J. Agric. Res. 2009, 7, 96–106.
22. Nikolopoulou, D.,; Grigorakis, K.,; Stasini, M.,; Alexis, M.N.,; Iliadis, K., Differences in chemical composition of field pea (Pisum sativum) cultivars: Effects of cultivation area and year. Food Chem. 2007, 103, 847–852.
23. Wang, N.,; Hatcher, D.W.,; Warkentin, T.D.,; Toews, R., Effect of cultivar and environment on physicochemical and cooking characteristics of field pea (Pisum sativum). Food Chem. 2010, 118, 109–115.
24. Al-Karaki, G.N.,; Ereifej, K.I., Relationships between seed yield and chemical composition of field peas grown under semi-arid Mediterranean conditions. J. Agron. Crop Sci., 1999, 182, 279–284.
25. McLean, L.A.,; Sosulski, F.W.,; Youngs, C.G., Effects of nitrogen and moisture on yield and protein in field peas. Can. J. Plant Sci. 1974, 54, 301–305.
26. Sosulski, F.W.,; McLean, L.A.,; Austenson, H.M., Management for yield and protein of field peas in Saskatchewan. Can. J. Plant Sci. 1974, 54, 247–251.
27. Reichert, R.D.,; MacKenzie, S.L., Composition of peas (Pisum sativum) varying widely in protein content. J. Agric. Food Chem. 1982, 30, 312–317.
28. Cousin, R., Peas (Pisum sativum L.). Field Crops Res. 1997, 53, 111–130.
29. Schroeder, H.E., Quantitative studies on the cotyledonary proteins in the genus Pisum. J. Sci. Food Agric. 1982, 33, 623–633.
30. Chandler, P.M.,; Spencer, D.,; Randall, P.J.,; Higgins, T.J., Influence of sulfur nutrition on developmental patterns of some major pea seed proteins and their mRNAs. Plant Physiol. 1984, 75, 651–657.
31. Danielsson, C.E., A contribution to the study of the synthesis of the reserve proteins in ripening pea seeds. Acta Chem. Scand. 1952, 6, 149–159.
32. Wright, D.J.,; Boulter, D., The characterisation of vicilin during seed development in Vicia faba (L.). Planta (Berl.) 1972, 105, 60–65.
33. st European Conference on Grain Legumes: Angers, France, 1992; pp 53–54.
34. Svedberg, T., The ultra-centrifuge and the study of high-molecular compounds. Nature 1937, 139(3529), 1051–1062.
35. Chambers, S.J.,; Bacon, J.R.,; Lambert, N., The quantitative analysis of seed proteins from peas using high performance liquid chromatography. Phytochem. Anal. 1992, 3, 49–54.
36. Casey, R., Immunoaffinity chromatography as a means of purifying legumin from Pisum (pea) seeds. Biochem. J. 1979, 177, 509–520.
37. Laurell, C.B., Quantitative estimation of proteins by electrophoresis in agarose gel containing antibodies. Anal. Biochem. 1966, 15, 45–52.
38. th ed.); Springer Science + Business Media, LLC: New York, NY, 2010; p 602.
39. Hoang, H.D., Evaluation of pea protein and modified pea protein as egg replacers (Doctoral dissertation); North Dakota State University: Fargo, ND, 2012.
40. Anson, M.L.,; Pader, M., U.S. Patent No. 2,785,155; U.S. Patent and Trademark Office: Washington, DC, 1957.
41. Hall, G.M., Methods of testing protein functionality; Blackie Academic & Professional: London, UK, 1996; p 265.
42. Fabian, C.,; Ju, Y.H., A review on rice bran protein: its properties and extraction methods. Critical Reviews in Food Science and Nutrition 2011, 51, 816–827.
43. Cone, C.N.,; Brown, E.D., U.S. Patent No. 1,955,375; U.S. Patent and Trademark Office: Washington, DC, 1934.
44. Owusu-Ansuh, Y.J.,; McCurdy, S.M.,; Fedec, P., Technical project report to Saskatchewan Agriculture Development Fund and AgDevCo: Pea proteins: a review on chemistry, technology of production and utilization (Report No. R-86-08-0037); POS, 1987.
45. Boye, J.I.,; Askay, S.,; Roufik, S.,; Ribéreau, S.,; Mondor, M.,; Farnworth, E.,; Rajamohamad, S.H., Comparison of the functional properties of pea, chickpea and lentil protein concentrates processed using ultrafiltration and isoelectric precipitation techniques. Food Res. Int. 2010, 43, 537–546.
46. Mondor, M.,; Tuyishime, O.,; Droplet, H., Production of pea protein concentrates by ultrafiltration: influence of hollow-fibre module. Innovative Food Sci. Emerging Technol. 2012, 14, 135–138.
47. Murray, E.D.,; Barker, L.D.,; Myers, C.D., Canada Patent No. 1,028,552; Canadian Intellectual Property Office: Gatineau, QC, 1978.
48. Gueguen, J.K.,; Barbot, J., Quantitative and qualitative variability of pea (Pisum sativum L.) protein composition. J. Sci. Food Agric. 1988, 42, 209–224.
49. th ed.); W.H. Freeman: New York, NY, 2002; p 1050.
50. Jain, S.M., U.S. Patent No. 4,321,192; U.S. Patent and Trademark Office: Washington, DC, 1892.
51. Ahmed, H., Principles and reactions of protein extraction, purification, and characterization; CRC Press LLC: Boca Raton, FL, 2005.
52. Crévieu, I.,; Bérot, S.,; Guéguen, J., Large scale procedure for fractionation of albumins and globulins from pea seeds. Nahrung 1996, 40(5), 237–244.
53. Mwasaru, M.A.,; Muhammad, K.,; Bakar, J.,; Che Man, Y.B., Effects of isolation technique and conditions on the extractability, physicochemical and functional properties of pigeonpea (Cajanus cajan) and cowpea (Vigna unguiculata) protein isolates: I. Physicochemical properties. Food Chem. 1999, 67, 435–443.
54. Smith, A.K.,; Rackis, J.J.,; Isnardi, P.,; Cartter, J.L.,; Krober, O.A., Nitrogen solubility index, isolated protein yield, and whey nitrogen content of several soybean strains. Cereal Chem. 1966, 43, 261–270.
55. th ed.); Taylor & Francis Group, LLC: Boca Raton, FL, 2008; p 1160.
56. Alzagtat, A.A.,; Alli, I., Protein-lipid interactions in food systems: A review. International J. Food Sci. Nutr. 2002, 53, 249–260.
57. Zayas, J.F., Functionality of proteins in food; Springer-Verlag Berlin Heidelberg: Germany, 1997; p 373.
58. Taherian, A.R.,; Mondor, M.,; Labranche, J.,; Drolet, H.,; Ippersiel, D.,; Lamarche, F., Comparative study of functional properties of commercial and membrane processed yellow pea protein isolates. Food Res Int 2011, 44, 2505–2514.
59. Withana-Gamage, T.S.,; Wanasundara, J.P.D.,; Pietrasik, Z.,; Shand, P.J., Physiochemical, thermal and functional characterisation of protein isolates from Kabuli and Desi chickpea (Cicer arietinum L.): a comparative study with soy (Glycine max) and pea (Pisum sativum L.). J. Sci. Food Agric. 2011, 91, 1022–1031.
60. Vose, J.R., Production and functionality of starches and protein isolates from legume seeds (field peas and horsebeans). Cereal Chem. 1980, 57(6), 406–410.
61. Walstra, P., Physical chemistry of foods; Marcel Dekker, Inc.: New York, NY, 2003; p 832.
62. Stone, A.K.,; Avramenko, N.A.,; Warkentin, T.D.,; Nickerson, M.T., Functional properties of protein isolates from different pea cultivars. Food Sci. Biotechnol. 2015, 24, 827–833.
63. Fuhrmeister, H.,; Meuser, F., Impact of processing on functional properties of protein products from wrinkled peas. J. Food Eng. 2003, 56, 119–129.
64. Lin, C.S.,; Zayas, J.F., Functionality of defatted corn germ proteins in a model system: fat binding capacity and water retention. J. Food Sci. 1987, 52(5), 1308–1311.
65. Sanjeewa, W.T., Physico-chemical properties of chickpea flour, starch and protein fractions and their utilization in low-fat pork bologna (Masters dissertation); University of Saskatchewan: Saskatoon, SK, 2008.
66. Soetrisno, U.S.S.,; Holmes, Z., Functional properties of acid and salt extracted proteins of yellow peas (Pisum sativum L. Miranda). J. Agric. Food Chem. 1992, 40, 975–980.
67. Damodaran, S., Protein stabilization of emulsions and foams. J. Food Sci. 2005, 70(3), R54–R66.
68. Dickinson, E., Flocculation of protein-stabilized oil-in-water emulsions. Colloids Surf., B 2010, 81, 130–140.
69. Lam, R.S.H.,; Nickerson, M.T., The effect of pH and heat pre-treatments on the physicochemical and emulsifying properties of β-lactoglobulin. Food Biophys. 2014, 9, 20–28.
70. McClements, D.J., Protein-stabilized emulsions. Curr. Opin. Colloid Interface Sci. 2004, 9, 305–313.
71. Liu, S.,; Elmer, C.,; Low, N.H.,; Nickerson, M.T., Effect of pH on the functional behaviour of pea protein isolate-gum Arabic complexes. Food Res. Int. 2010, 43, 489–495.
72. Barac, M.,; Cabrilo, S.,; Pesic, M.,; Stanojevic, S.,; Zilic, S.,; Macej, O.,; Ristic, N., Profile and functional properties of seed proteins from six pea (Pisum sativum) genotypes. International J. Mol. Sci. 2010, 11, 4973–4990.
73. Aluko, R.E.,; Mofolasayo, O.A.,; Watts, B.M., Emulsifying and foaming properties of commercial yellow pea (Pisum sativum L.) seed flours. J. Agric. Food Chem. 2009, 57, 9793–9800.
74. Dagorn-Scaviner, C.,; Gueguen, J.,; Lefebvre, J., Emulsifying properties of pea globulins as related to their absorption behaviours. J. Food Sci. 1987, 52, 335–341.
75. Liang, H.-N.,; Tang, C.-H., pH-dependent emulsifying properties of pea [Pisum sativum (L.)] proteins. Food Hydrocolloids 2013, 33, 309–319.
76. Gharsallaoui, A.,; Cases, E.,; Chambin, O.,; Saurel, R., Interfacial and emulsifying characteristics of acid-treated pea protein. Food Biophys. 2009, 4, 273–280.
77. Tsoukala, A.,; Papalamprou, E.,; Mari, E.,; Doxatakis, G.,; Braudo, E.E., Absorption at the air-water interface and emulsification properties of grain legume protein derivatives from pea and broad bean. Colloids Surf., B 2006, 53, 203–208.
78. Wierenga, P.A.,; Gruppen, H., New views on foams from protein solutions. Curr. Opin. Colloid Interface Sci. 2010, 15, 365–373.
79. Kinsella, J.E., Functional properties of proteins: possible relationships between structure and function in foams. Food Chem. 1981, 7, 273–288.
80. Ikeda, S.,; Nishinari, K., On solid-like rheological behaviors of globular protein solutions. Food Hydrocolloids 2001, 15, 401−406.
81. Corredig, M., Protein-Protein Interactions in Food. In Ingredient Interactions: Effects on Food Quality, Second Edition; Gaonkar, A.G., McPherson, A., Eds.; Taylor & Francis Group, LLC: Boca Raton, FL, 2006.
82. Culbertson, J.D., Food Protein Functionality. In Handbook of Food Science, Technology, and Engineering, Volume 1; Hui, Y.H., Ed.; Taylor & Francis Group, LLC: Boca Raton, FL, 2005.
83. O’Kane, F.E.,; Happe, R.P.,; Vereijken, J.M.,; Gruppen, H., van Boekel, M.A.J.S., Characterization of pea vicilin. 2. Consequences of compositional heterogeneity on heat-induced gelation behavior. J. Agric. Food Chem. 2004, 52, 3149−3154.
84. Shand, P.J.,; Ya, H.,; Pietrasik, Z.,; Wanasundara, P.K.J.P.D., Physicochemical and textural properties of heat-induced pea protein isolate gels. Food Chem. 2007, 102, 1119−1130.
85. Sun, X.D.,; Arntfield, S., Dynamic oscillatory rheological measurement and thermal properties of pea protein extracted by salt method: Effect of pH and NaCl. J. Food Eng. 2011, 105, 577−582.
86. Mession, J.,; Sok, N.,; Assifaoui, A.,; Saurel, R., Thermal denaturation of pea globulins (Pisum sativum L.)-molecular interactions leading to heat-induced protein aggregation. J. Agric. Food Chem. 2013, 61, 1196−1204.
87. Munialo, C.D.,; van der Linden, E.,; Akt, K.,; de Jongh, H.H.J., Quantitative analysis of the network structure that underlines the transitioning in mechanical responses of pea protein gels. Food Hydrocolloids 2015, 49, 104−117.
88. Mession, J.,; Blanchard, C.,; Mint-Dah, F.,; Lafarge, C.,; Assifaoui, A.,; Saurel, R., The effects of sodium alginate and calcium levels on pea proteins cold-set gelation. Food Hydrocolloids 2013, 31, 446−457.
89. Foegeding, E.A.,; Davis, J., P. Food protein functionality: a comprehensive approach. Food Hydrocolloids 2010, 25, 1853–1864.
90. Mession, J.,; Chihi, M.L.,; Sok, N., Saurel, R., Effect of globular pea proteins fractionation on their heat-induced aggregation and acid cold-set gelation. Food Hydrocolloids 2015, 46, 233−243.
91. Koyoro, H.,; Powers, J.R., Functional properties of pea globulin fractions. Cereal Chem. 1987, 64(2), 97–101.
92. Dagorn-Scaviner, C.,; Gueguen, J.,; Lefebvre, J., A comparison of interfacial behaviours of pea (Pisum sativum L.) legumin and vicilin at air/water interface. Die Nahrung. 1986, 30, 337–347.
93. Cserhalmi, Zs.; Czukor, B.,; Gajzágó-Schuster, I., Emulsifying properties, surface hydrophobicity and thermal denaturation of pea protein isolates. Acta Aliment. 1998, 27, 357–363.
94. Derbyshire, E.,; Wright, D.J.,; Boulter, D., Review: legumin and vicilin, storage proteins of legume seeds. Phytochemistry 1976, 15, 3–24.
95. Krishna, T.G.,; Croy, R.R.D.,; Boulter, D., Heterogeneity in subunit composition of the legumin of Pisum sativum. Phytochemistry 1979, 18, 1879–1880.
96. Kimura, A.,; Fukuda, T.,; Zhang, M.,; Motoyama, S.,; Maruyama, N.,; Utsumi, S., Comparison of physicochemical properties of 7S and 11S globulins from pea, fava bean, cowpea, and French bean with those of soybean–French bean 7S globulin exhibits excellent properties. Journal of Agricultural and Food Chemistry 2008, 56, 10273–10279.