Rapid, selection-free, high-efficiency genome editing in protozoan parasites using CRISPR-cas9 ribonucleoproteins

mBio

Volumn 8, Issue 6, 2017, Pages

  Medeiros, Lia Carolina Soares ^a,b   South, Lilith ^a   Peng, Duo ^a   Bustamante, Juan M ^a   Wang, Wei ^a   Bunkofske, Molly ^a   Perumal, Natasha ^a   Sanchez Valdez, Fernando ^a   Tarleton, Rick L ^a  

^a UNIVERSITY OF GEORGIA   (United States)

^b INSTITUTO CARLOS CHAGAS   (Brazil)

Author keywords

Cas9; CRISPR; Genome editing; Leishmania; Ribonucleoproteins; SaCas9; Trypanosoma; Trypanosoma brucei; Trypanosoma cruzi

Indexed keywords

RIBONUCLEOPROTEIN; RNA; SINGLE GUIDE RNA; UNCLASSIFIED DRUG;

ARTICLE; CONTROLLED STUDY; CRISPR-CAS9 SYSTEM; EPIMASTIGOTE; GENE EDITING; GENE FUNCTION; GENE INACTIVATION; GENE TARGETING; IN VITRO STUDY; KINETOPLASTID LIFE CYCLE STAGE; LEISHMANIA MAJOR; NONHUMAN; PRIORITY JOURNAL; PROTOZOON; RECOMBINATION REPAIR; REPORTER GENE; STAPHYLOCOCCUS AUREUS; STREPTOCOCCUS PYOGENES; TRYPANOSOMA BRUCEI; TRYPANOSOMA CRUZI; CRISPR CAS SYSTEM; ENZYMOLOGY; GENE; GENE KNOCKOUT; GENETICS; GROWTH, DEVELOPMENT AND AGING; LIFE CYCLE STAGE; PROCEDURES; TRYPANOSOMA BRUCEI BRUCEI; TRYPANOSOMATIDAE;

CRISPR-CAS SYSTEMS; GENE EDITING; GENE KNOCKOUT TECHNIQUES; GENES, PROTOZOAN; LEISHMANIA MAJOR; LIFE CYCLE STAGES; RIBONUCLEOPROTEINS; STAPHYLOCOCCUS AUREUS; TRYPANOSOMA BRUCEI BRUCEI; TRYPANOSOMA CRUZI; TRYPANOSOMATINA;

EID: 85036530302     PISSN: 21612129     EISSN: 21507511     Source Type: Journal    
DOI: 10.1128/mBio.01788-17     Document Type: Article

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