Evaluation of high efficiency gene knockout strategies for Trypanosoma cruzi

BMC Microbiology

Volumn 9, Issue , 2009, Pages

  Xu, Dan ^a   Pérez Brandán, Cecilia ^b   Basombrío, Miguel Angel ^b   Tarleton, Rick L ^a  

^a UNIVERSITY OF GEORGIA   (United States)

^b UNIVERSIDAD NACIONAL DE SALTA   (Argentina)

Author keywords

[No Author keywords available]

Indexed keywords

DIHYDROFOLATE REDUCTASE THYMIDYLATE SYNTHASE; NUCLEOTIDE DERIVATIVE; PROTEIN ECH1; PROTEIN ECH2; PROTOZOAL PROTEIN; UNCLASSIFIED DRUG;

ALLELE; ARTICLE; CONTROLLED STUDY; GENE DELETION; GENE SEQUENCE; GENE TARGETING; NONHUMAN; POLYMERASE CHAIN REACTION; PROTEIN DOMAIN; TRYPANOSOMA CRUZI; ANIMAL; COMPARATIVE STUDY; EVALUATION; GENE INACTIVATION; GENETICS; METHODOLOGY; SENSITIVITY AND SPECIFICITY;

KINETOPLASTIDA; PROTOZOA; TRYPANOSOMA CRUZI;

ANIMALS; GENE DELETION; GENE KNOCKOUT TECHNIQUES; SENSITIVITY AND SPECIFICITY; TRYPANOSOMA CRUZI;

EID: 66949138915     PISSN: 14712180     EISSN: 14712180     Source Type: Journal    
DOI: 10.1186/1471-2180-9-90     Document Type: Article

References (38)

1. The trypanosomiases. MP Barrett RJ Burchmore A Stich JO Lazzari AC Frasch JJ Cazzulo S Krishna, Lancet 2003 362 9394 1469 1480 14602444 (Pubitemid 37352887)
2. Control of Chagas disease. World Health Organ Tech Rep Ser 2002 905 i iv 1-109, back cover 12092045
3. Reporte sobre la enfermedad de Chagas. TDR/PAHO/WHO Scientific Working Group Report, 2007 http://www.who.int/tdr/svc/publications/tdr-research- publications/reporte-enfermedad-chagas
4. The life cycle of Trypanosoma cruzi revisited. KM Tyler DM Engman, Int J Parasitol 2001 31 5-6 472 481 11334932 (Pubitemid 32382045)
5. The Genome Sequence of Trypanosoma cruzi, Etiologic Agent of Chagas Disease. NM El-Sayed PJ Myler DC Bartholomeu D Nilsson G Aggarwal A-N Tran E Ghedin EA Worthey AL Delcher G Blandin, Science 2005 309 5733 409 415 16020725
6. Functional mapping of a trypanosome centromere by chromosome fragmentation identifies a 16-kb GC-rich transcriptional "strand- switch" domain as a major feature. SO Obado MC Taylor SR Wilkinson EV Bromley JM Kelly, Genome Res 2005 15 1 36 43 15632088
7. Nucleotide sequences provide evidence of genetic exchange among distantly related lineages of Trypanosoma cruzi. CA Machado FJ Ayala, Proc Natl Acad Sci U S A 2001 98 13 7396 7401 11416213 (Pubitemid 32567960)
8. Deletion of an immunodominant Trypanosoma cruzi surface glycoprotein disrupts flagellum-cell adhesion. R Cooper AR de Jesus GA Cross, J Cell Biol 1993 122 1 149 156 8314840
9. The calmodulin-ubiquitin (CUB) genes of Trypanosoma cruzi are essential for parasite viability. J Ajioka J Swindle, Mol Biochem Parasitol 1996 78 1-2 217 225 8813691 (Pubitemid 26201619)
10. Oligopeptidase B-dependent signaling mediates host cell invasion by Trypanosoma cruzi. EV Caler S Vaena de Avalos PA Haynes NW Andrews BA Burleigh, Embo J 1998 17 17 4975 4986 9724634 (Pubitemid 28408464)
11. Intracellular growth and metacyclogenesis defects in Trypanosoma cruzi carrying a targeted deletion of a Tc52 protein-encoding allele. A Allaoui C Francois K Zemzoumi E Guilvard A Ouaissi, Mol Microbiol 1999 32 6 1273 1286 10383767 (Pubitemid 29286666)
12. LYT1 protein is required for efficient in vitro infection by Trypanosoma cruzi. R Manning-Cela A Cortes E Gonzalez-Rey WC Van Voorhis J Swindle A Gonzalez, Infect Immun 2001 69 6 3916 3923 11349059 (Pubitemid 32493313)
13. The interplay between folding-facilitating mechanisms in Trypanosoma cruzi endoplasmic reticulum. I Conte C Labriola JJ Cazzulo R Docampo AJ Parodi, Mol Biol Cell 2003 14 9 3529 3540 12972544 (Pubitemid 37151606)
14. The origin of dihydroorotate dehydrogenase genes of kinetoplastids, with special reference to their biological significance and adaptation to anaerobic, parasitic conditions. T Annoura T Nara T Makiuchi T Hashimoto T Aoki, J Mol Evol 2005 60 1 113 127 15696374 (Pubitemid 40310764)
15. The suppression of galactose metabolism in Trypanosoma cruzi epimastigotes causes changes in cell surface molecular architecture and cell morphology. JI MacRae SO Obado DC Turnock JR Roper M Kierans JM Kelly MAJ Ferguson, Mol Biochem Parasitol 2006 147 1 126 136 16569451
16. Trypanosoma cruzi: Attenuation of virulence and protective immunogenicity after monoallelic disruption of the cub gene. AB Barrio WC Van Voorhis MA Basombro, Experimental Parasitology 2007 117 4 382 389 17624327 (Pubitemid 350050953)
17. The Trypanosoma cruzi metacyclic-specific protein Met-III associates with the nucleolus and contains independent amino and carboxyl terminal targeting elements. E Gluenz MC Taylor JM Kelly, Int J Parasitol 2007 37 6 617 625 17239886 (Pubitemid 46440939)
18. A mechanism for cross-resistance to nifurtimox and benznidazole in trypanosomes. SR Wilkinson MC Taylor D Horn JM Kelly I Cheeseman, Proc Natl Acad Sci USA 2008 105 13 5022 5027 18367671
19. Genetic manipulation of kinetoplastida. CE Clayton, Parasitol Today 1999 15 9 372 378 10461166 (Pubitemid 29392918)
20. Tests of cytoplasmic RNA interference (RNAi) and construction of a tetracycline-inducible T7 promoter system in Trypanosoma cruzi. WD DaRocha K Otsu SMR Teixeira JE Donelson, Mol Biochem Parasitol 2004 133 2 175 186 14698430 (Pubitemid 38032866)
21. RNA interference as a genetic tool in trypanosomes. V Bellofatto JB Palenchar, Methods Mol Biol 2008 442 83 94 18369780
22. Cloning and expression of the dihydrofolate reductase-thymidylate synthase gene from Trypanosoma cruzi. P Reche R Arrebola A Olmo DV Santi D Gonzalez-Pacanowska LM Ruiz-Perez, Mol Biochem Parasitol 1994 65 2 247 258 7969266 (Pubitemid 24229070)
23. Expression and characterization of the Trypanosoma cruzi dihydrofolate reductase domain. P Reche R Arrebola DV Santi D Gonzalez-Pacanowska LM Ruiz-Perez, Mol Biochem Parasitol 1996 76 1-2 175 185 8920005 (Pubitemid 26122733)
24. Targeting DHFR in parasitic protozoa. AC Anderson, Drug Discov Today 2005 10 2 121 128 15718161 (Pubitemid 40247810)
25. Double targeted gene replacement for creating null mutants. A Cruz CM Coburn SM Beverley, Proc Natl Acad Sci USA 1991 88 16 7170 7174 1651496 (Pubitemid 21915111)
26. Chemical and genetic validation of dihydrofolate reductase-thymidylate synthase as a drug target in African trypanosomes. N Sienkiewicz S Jaroslawski S Wyllie AH Fairlamb, Mol Microbiol 2008 69 2 520 533 18557814
27. A transformation vector for stage-specific expression of heterologous genes in Trypanosoma cruzi epimastigotes. MC Thomas A Gonzalez, Parasitol Res 1997 83 2 151 156 9039697 (Pubitemid 27080885)
28. The Trypanosoma cruzi proteome. JA Atwood 3rd DB Weatherly TA Minning B Bundy C Cavola FR Opperdoes R Orlando RL Tarleton, Science 2005 309 5733 473 476 16020736
29. Genetic regulation of protein synthesis in trypanosomes. A Das V Bellofatto, Curr Mol Med 2004 4 6 577 584 15357209 (Pubitemid 39172364)
30. Control of gene expression and genetic manipulation in the Trypanosomatidae. SM Teixeira WD daRocha, Genet Mol Res 2003 2 1 148 158 12917811 (Pubitemid 38846558)
31. Effects of 3' untranslated and intergenic regions on gene expression in Trypanosoma cruzi. T Nozaki GA Cross, Mol Biochem Parasitol 1995 75 1 55 67 8720175
32. Parameters controlling the rate of gene targeting frequency in the protozoan parasite Leishmania. B Papadopoulou C Dumas, Nucleic Acids Res 1997 25 21 4278 4286 9336458 (Pubitemid 27485206)
33. Polymerase chain reaction-based gene disruption in Trypanosoma brucei. A Gaud M Carrington J Deshusses DR Schaller, Mol Biochem Parasitol 1997 87 1 113 115 9233680 (Pubitemid 27293341)
34. Simple one-week method to construct gene-targeting vectors: application to production of human knockout cell lines. S Iiizumi Y Nomura S So K Uegaki K Aoki K Shibahara N Adachi H Koyama, BioTechniques 2006 41 3 311 316 16989091 (Pubitemid 46534894)
35. Flagellar elongation induced by glucose limitation is preadaptive for Trypanosoma cruzi differentiation. KM Tyler DM Engman, Cell Motil Cytoskeleton 2000 46 4 269 278 10962481
36. A Shuttle Vector Which Facilitates the Expression of Transfected Genes in Trypanosoma-Cruzi and Leishmania. JM Kelly HM Ward MA Miles G Kendall, Nucleic Acids Research 1992 20 15 3963 3969 1324472
37. Integration of expression vectors into the ribosomal locus of Trypanosoma cruzi. HA Lorenzi MP Vazquez MJ Levin, Gene 2003 310 91 99 12801636 (Pubitemid 36703303)
38. Molecular Cloning. A Laboratory Manual. J Sambrook DW Russel, Cold Spring Harbor Laboratory Press 3 2001 1