First efficient CRISPR-Cas9-mediated genome editing in Leishmania parasites

Cellular Microbiology

Volumn 17, Issue 10, 2015, Pages 1405-1412

  Sollelis, Lauriane ^a,b   Ghorbal, Mehdi ^a,b   Macpherson, Cameron Ross ^c   Martins, Rafael Miyazawa ^c   Kuk, Nada ^a   Crobu, Lucien ^b   Bastien, Patrick ^a,b,d   Scherf, Artur ^c   Lopez Rubio, Jose Juan ^a,b   Sterkers, Yvon ^a,b,d  

^a UNIV MONTPELLIER   (France)

^b University de Montpellier   (France)

^c INSTITUT PASTEUR   (France)

^d MONTPELLIER UNIVERSITY HOSPITAL   (France)

Author keywords

[No Author keywords available]

Indexed keywords

DIHYDROFOLATE REDUCTASE THYMIDYLATE SYNTHASE; GUIDE RNA; THYMIDYLATE SYNTHASE; UNCLASSIFIED DRUG;

ARTICLE; CELL POPULATION; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEAT; COMPARATIVE STUDY; GENE INACTIVATION; GENETIC MANIPULATION; GENETIC TRANSFECTION; LEISHMANIA; MULTIGENE FAMILY; NONHUMAN; PLASMODIUM FALCIPARUM; PRIORITY JOURNAL; PROMOTER REGION; WESTERN BLOTTING; CRISPR CAS SYSTEM; GENE DELETION; GENE TARGETING; GENETIC RECOMBINATION; GENETICS; GENOME; MOLECULAR BIOLOGY; PARASITOLOGY; PROCEDURES;

CRISPR-CAS SYSTEMS; GENE DELETION; GENE TARGETING; GENOME, PROTOZOAN; LEISHMANIA; MOLECULAR BIOLOGY; PARASITOLOGY; RECOMBINATION, GENETIC;

EID: 84942194082     PISSN: 14625814     EISSN: 14625822     Source Type: Journal    
DOI: 10.1111/cmi.12456     Document Type: Article

References (22)

1. den Boer, M., Argaw, D., Jannin, J., and Alvar, J. (2011) Leishmaniasis impact and treatment access. Clin Microbiol Infect 17: 1471-1477.
2. Cong, L., Ran, F.A., Cox, D., Lin, S., Barretto, R., Habib, N., etal. (2013) Multiplex genome engineering using CRISPR/Cas systems. Science 339: 819-823.
3. Dubessay, P., Ravel, C., Bastien, P., Crobu, L., Dedet, J.P., Pages, M., and Blaineau, C. (2002) The switch region on Leishmania major chromosome 1 is not required for mitotic stability or gene expression, but appears to be essential. Nucleic Acids Res 30: 3692-3697.
4. Dubessay, P., Blaineau, C., Bastien, P., and Pages, M. (2004) Chromosome fragmentation in leishmania. Methods Mol Biol 270: 353-378.
5. Dubessay, P., Blaineau, C., Bastien, P., Tasse, L., Van Dijk, J., Crobu, L., and Pages, M. (2006) Cell cycle-dependent expression regulation by the proteasome pathway and characterization of the nuclear targeting signal of a Leishmania major Kin-13 kinesin. Mol Microbiol 59: 1162-1174.
6. Gaj, T., Gersbach, C.A., and Barbas, C.F., 3rd (2013) ZFN, TALEN, and CRISPR/Cas-based methods for genome engineering. Trends Biotechnol 31: 397-405.
7. Ghorbal, M., Gorman, M., Macpherson, C.R., Martins, R.M., Scherf, A., and Lopez-Rubio, J.J. (2014) Genome editing in the human malaria parasite Plasmodium falciparum using the CRISPR-Cas9 system. Nat Biotechnol 32: 819-821.
8. Gilles, A.F., and Averof, M. (2014) Functional genetics for all: engineered nucleases, CRISPR and the gene editing revolution. Evodevo 5: 43.
9. Hsu, P.D., Scott, D.A., Weinstein, J.A., Ran, F.A., Konermann, S., Agarwala, V., etal. (2013) DNA targeting specificity of RNA-guided Cas9 nucleases. Nat Biotechnol 31: 827-832.
10. Ismach, R., Cianci, C.M., Caulfield, J.P., Langer, P.J., Hein, A., and McMahon-Pratt, D. (1989) Flagellar membrane and paraxial rod proteins of Leishmania: characterization employing monoclonal antibodies. J Protozool 36: 617-624.
11. Jinek, M., Chylinski, K., Fonfara, I., Hauer, M., Doudna, J.A., and Charpentier, E. (2012) A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity. Science 337: 816-821.
12. Lachaud, L., Bourgeois, N., Kuk, N., Morelle, C., Crobu, L., Merlin, G., etal. (2014) Constitutive mosaic aneuploidy is a unique genetic feature widespread in the Leishmania genus. Microbes Infect 16: 61-66.
13. Lye, L.F., Owens, K., Shi, H., Murta, S.M., Vieira, A.C., Turco, S.J., etal. (2010) Retention and loss of RNA interference pathways in trypanosomatid protozoans. PLoS Pathog 6: e1001161.
14. MacPherson, C.R., and Scherf, A. (2015) Protospacer Workbench: flexible guide-RNA design for CRISPR applications. Nat Biotechnol. In press.
15. Maga, J.A., and LeBowitz, J.H. (1999) Unravelling the kinetoplastid paraflagellar rod. Trends Cell Biol 9: 409-413.
16. Nakaar, V., Dare, A.O., Hong, D., Ullu, E., and Tschudi, C. (1994) Upstream tRNA genes are essential for expression of small nuclear and cytoplasmic RNA genes in trypanosomes. Mol Cell Biol 14: 6736-6742.
17. Passos-Silva, D.G., Rajao, M.A., Nascimento de Aguiar, P.H., Vieira-da-Rocha, J.P., Machado, C.R., and Furtado, C. (2010) Overview of DNA repair in Trypanosoma cruzi, Trypanosoma brucei, and Leishmania major. J Nucleic Acids 2010: 840768.
18. Peng, D., Kurup, S.P., Yao, P.Y., Minning, T.A., and Tarleton, R.L. (2015) CRISPR-Cas9-mediated single-gene and gene family disruption in Trypanosoma cruzi. MBio 6: e02097-14.
19. Santrich, C., Moore, L., Sherwin, T., Bastin, P., Brokaw, C., Gull, K., and LeBowitz, J.H. (1997) A motility function for the paraflagellar rod of Leishmania parasites revealed by PFR-2 gene knockouts. Mol Biochem Parasitol 90: 95-109.
20. Shen, B., Brown, K.M., Lee, T.D., and Sibley, L.D. (2014) Efficient gene disruption in diverse strains of Toxoplasma gondii using CRISPR/CAS9. MBio 5: e01114.
21. Sterkers, Y., Lachaud, L., Crobu, L., Bastien, P., and Pagès, M. (2011) FISH analysis reveals aneuploidy and continual generation of chromosomal mosaicism in Leishmania major. Cell Microbiol 13: 274-283.
22. Wagner, J.C., Platt, R.J., Goldfless, S.J., Zhang, F., and Niles, J.C. (2014) Efficient CRISPR-Cas9-mediated genome editing in Plasmodium falciparum. Nat Methods 11: 915-918.